BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0206.0013.p (360 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770596.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhizobi... 572 e-161 ref|YP_577799.1| enoyl-CoA hydratase [Nitrobacter hamburgensis X... 543 e-152 ref|YP_001205172.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhiz... 527 e-147 ref|YP_001239576.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhiz... 525 e-147 ref|ZP_01046631.1| enoyl-CoA hydratase [Nitrobacter sp. Nb-311A]... 520 e-145 >ref|NP_770596.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC49221.1| enoyl-CoA hydratase [Bradyrhizobium japonicum USDA 110] Length = 355 Score = 572 bits (1473), Expect = e-161, Method: Compositional matrix adjust. Identities = 286/352 (81%), Positives = 310/352 (88%) Query: 5 AAAEGDLIARKEGSAGIIRLNRPKAINAVTLEMFHDIDRALDLFEADGDVAVVVLEGAGD 64 AA EGDL+AR EGSAG+IRLNRPKAINAVTLEMF DI+ ALD FE D VAV+VLEGAG+ Sbjct: 3 AAEEGDLVARVEGSAGVIRLNRPKAINAVTLEMFRDIETALDRFETDAAVAVIVLEGAGE 62 Query: 65 RGLCAGGDIRALWESSKVKGDLGKILWRDEYILNARIKKFPKPYVAFMDGIVMGGGVGLS 124 RGLCAGGDIR LWESSKV GDLGKILWR+EYILNARIK+FPKPYVAFMDGIVMGGGVGLS Sbjct: 63 RGLCAGGDIRTLWESSKVHGDLGKILWREEYILNARIKRFPKPYVAFMDGIVMGGGVGLS 122 Query: 125 AHSRHRVVTERTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGTTMNGPDAIYAK 184 AH+ HRVVT+RTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTG TMNGPDAI+AK Sbjct: 123 AHASHRVVTDRTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGQTMNGPDAIHAK 182 Query: 185 FADAVVPSAKLPALCEALTKVAPGTGSAEIDRLIGGFATGEKSGPVAAMQAKIDGWFARG 244 FAD VVP+AK P L +ALTK+ G +AE+ +LI GFATGE +GPVAA + ID F Sbjct: 183 FADWVVPAAKWPELRDALTKLRSGATAAEVGKLINGFATGETAGPVAAKEPMIDALFGFD 242 Query: 245 RMEDVVAALTADGSDMARDTLKTLGEKSPRGMVVTLKLLRLARATATLEECLVREYRAAL 304 RMED+VAAL DGS+ A TLKTLGEKSPRGMVVTLKLLRLARA ++LEECLVREYRAAL Sbjct: 243 RMEDIVAALKRDGSEFALATLKTLGEKSPRGMVVTLKLLRLARAASSLEECLVREYRAAL 302 Query: 305 EVFASEDFREGVRAAVIDKDRNPTWSPARIEQVTPEMLAPYFAEIGADELKF 356 EVF S+DFREGVRAAVIDKDRNP WSP RIE VTPE+LAPY AEIGADELKF Sbjct: 303 EVFRSDDFREGVRAAVIDKDRNPIWSPPRIEDVTPELLAPYLAEIGADELKF 354 >ref|YP_577799.1| enoyl-CoA hydratase [Nitrobacter hamburgensis X14] gb|ABE63339.1| Enoyl-CoA hydratase/isomerase [Nitrobacter hamburgensis X14] Length = 357 Score = 543 bits (1398), Expect = e-152, Method: Compositional matrix adjust. Identities = 270/356 (75%), Positives = 301/356 (84%) Query: 1 MNDAAAAEGDLIARKEGSAGIIRLNRPKAINAVTLEMFHDIDRALDLFEADGDVAVVVLE 60 M A E DLIAR+EGSAGIIRLNRPKAINAVTLEMF +I ALD FEAD V +++LE Sbjct: 1 MKVIAGDETDLIARREGSAGIIRLNRPKAINAVTLEMFREIGSALDEFEADSAVGLILLE 60 Query: 61 GAGDRGLCAGGDIRALWESSKVKGDLGKILWRDEYILNARIKKFPKPYVAFMDGIVMGGG 120 GAG+RGLCAGGDIRALWES+K GDLGK+LWR+EYILNARI KFPKPYVAFMDGIVMGGG Sbjct: 61 GAGERGLCAGGDIRALWESAKEGGDLGKVLWREEYILNARIAKFPKPYVAFMDGIVMGGG 120 Query: 121 VGLSAHSRHRVVTERTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGTTMNGPDA 180 VGLSAH HRVVTERT+LAMPEVGLGFFPDVGGT+LLS +PGE GTYFGLTG MNGPDA Sbjct: 121 VGLSAHGGHRVVTERTRLAMPEVGLGFFPDVGGTWLLSHAPGETGTYFGLTGQPMNGPDA 180 Query: 181 IYAKFADAVVPSAKLPALCEALTKVAPGTGSAEIDRLIGGFATGEKSGPVAAMQAKIDGW 240 IYA FAD VPS KL AL EAL + ++ + I GFA+GE +GPVAA++ +ID W Sbjct: 181 IYAGFADVGVPSRKLAALREALVDLGTRADASRVKATIAGFASGETAGPVAAIRPRIDAW 240 Query: 241 FARGRMEDVVAALTADGSDMARDTLKTLGEKSPRGMVVTLKLLRLARATATLEECLVREY 300 FA GRM+D++AAL DGS++AR TLKTL EKSPRGMVVTLKLLRLARA+ATLEECL REY Sbjct: 241 FAHGRMDDIIAALNRDGSELARSTLKTLNEKSPRGMVVTLKLLRLARASATLEECLAREY 300 Query: 301 RAALEVFASEDFREGVRAAVIDKDRNPTWSPARIEQVTPEMLAPYFAEIGADELKF 356 RAAL VFAS+DFREGVRAAVIDKDR P WSP RI++VTPEM+APYFA IGADEL F Sbjct: 301 RAALAVFASDDFREGVRAAVIDKDREPKWSPPRIDEVTPEMVAPYFANIGADELVF 356 >ref|YP_001205172.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhizobium sp. ORS 278] emb|CAL76937.1| enoyl-CoA hydratase/isomerase [Bradyrhizobium sp. ORS 278] Length = 350 Score = 527 bits (1358), Expect = e-147, Method: Compositional matrix adjust. Identities = 269/350 (76%), Positives = 299/350 (85%) Query: 11 LIARKEGSAGIIRLNRPKAINAVTLEMFHDIDRALDLFEADGDVAVVVLEGAGDRGLCAG 70 +I R+EGSAGIIRLNRPKAINA+TLEM ID ALD FEAD +AVV+LEGAG+RGLCAG Sbjct: 1 MIVRREGSAGIIRLNRPKAINAMTLEMSIGIDAALDQFEADPAIAVVLLEGAGERGLCAG 60 Query: 71 GDIRALWESSKVKGDLGKILWRDEYILNARIKKFPKPYVAFMDGIVMGGGVGLSAHSRHR 130 GDIR L+ESSKV GDLGK+ WR EYI+NARI FPKPYVA+MDG+VMGGGVGLSAH RHR Sbjct: 61 GDIRGLYESSKVNGDLGKVFWRQEYIMNARIAAFPKPYVAYMDGLVMGGGVGLSAHGRHR 120 Query: 131 VVTERTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGTTMNGPDAIYAKFADAVV 190 +VTE+T+LAMPEVGLGFFPDVGGT+LLSR+PGE+G YFGLTG TMNGPDAI+A+FADAVV Sbjct: 121 IVTEKTRLAMPEVGLGFFPDVGGTWLLSRAPGELGAYFGLTGQTMNGPDAIHARFADAVV 180 Query: 191 PSAKLPALCEALTKVAPGTGSAEIDRLIGGFATGEKSGPVAAMQAKIDGWFARGRMEDVV 250 ++ PAL E LTKV PGT SAEIDRLI GFATGE GPVAA QAKID WFA M DV Sbjct: 181 ATSNWPALREVLTKVRPGTLSAEIDRLIAGFATGETEGPVAAHQAKIDAWFAHDSMHDVF 240 Query: 251 AALTADGSDMARDTLKTLGEKSPRGMVVTLKLLRLARATATLEECLVREYRAALEVFASE 310 AAL AD SD+A TLKTL EKSPRGMVVTLKLLRLAR +L+ECLVREYRAALEVF S+ Sbjct: 241 AALEADDSDLAASTLKTLKEKSPRGMVVTLKLLRLARGATSLQECLVREYRAALEVFRSD 300 Query: 311 DFREGVRAAVIDKDRNPTWSPARIEQVTPEMLAPYFAEIGADELKFPDSK 360 DFREGVRAA+IDKDRNP WSP ++QVTPEMLAPY AE G DEL FP ++ Sbjct: 301 DFREGVRAAIIDKDRNPKWSPPDVDQVTPEMLAPYLAENGPDELTFPQAR 350 >ref|YP_001239576.1| 3-hydroxyisobutyryl-CoA hydrolase [Bradyrhizobium sp. BTAi1] gb|ABQ35670.1| enoyl-CoA hydratase/isomerase [Bradyrhizobium sp. BTAi1] Length = 350 Score = 525 bits (1352), Expect = e-147, Method: Compositional matrix adjust. Identities = 266/350 (76%), Positives = 300/350 (85%) Query: 11 LIARKEGSAGIIRLNRPKAINAVTLEMFHDIDRALDLFEADGDVAVVVLEGAGDRGLCAG 70 +I R+EGSAGIIRLNRPKAINA+TLEM ID ALD FEAD VAVVVLEGAG+RGLCAG Sbjct: 1 MIVRREGSAGIIRLNRPKAINAMTLEMSMGIDAALDRFEADPAVAVVVLEGAGERGLCAG 60 Query: 71 GDIRALWESSKVKGDLGKILWRDEYILNARIKKFPKPYVAFMDGIVMGGGVGLSAHSRHR 130 GDIR L+ESSKV GDLGK+ WR EYI+NARI F KPY+A+MDG+VMGGGVGLSAH RHR Sbjct: 61 GDIRGLYESSKVNGDLGKVFWRQEYIMNARIAAFGKPYIAYMDGLVMGGGVGLSAHGRHR 120 Query: 131 VVTERTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGTTMNGPDAIYAKFADAVV 190 +VTE+T+LAMPEVGLGFFPDVGGT+LLSR+PGE+G YFGLTG TMNGPDAI+A+FADAVV Sbjct: 121 IVTEKTRLAMPEVGLGFFPDVGGTWLLSRAPGELGAYFGLTGQTMNGPDAIHARFADAVV 180 Query: 191 PSAKLPALCEALTKVAPGTGSAEIDRLIGGFATGEKSGPVAAMQAKIDGWFARGRMEDVV 250 P+ + PAL E LTKV PG S EID+LI GFATGE +GPVAA QAKID WFA RM D+ Sbjct: 181 PTERWPALREVLTKVRPGAVSLEIDQLIAGFATGETAGPVAAQQAKIDAWFAHDRMHDIF 240 Query: 251 AALTADGSDMARDTLKTLGEKSPRGMVVTLKLLRLARATATLEECLVREYRAALEVFASE 310 AL ADGS++A TLKTL EKSPRGMVVTLKLLRLAR +L+ECLVREYRAALEVF S+ Sbjct: 241 VALEADGSELALATLKTLKEKSPRGMVVTLKLLRLARKATSLQECLVREYRAALEVFRSD 300 Query: 311 DFREGVRAAVIDKDRNPTWSPARIEQVTPEMLAPYFAEIGADELKFPDSK 360 DFREGVRAA+IDKDRNP+WSP ++QVTPEML Y AEIGADEL FP ++ Sbjct: 301 DFREGVRAAIIDKDRNPSWSPPNVDQVTPEMLERYLAEIGADELTFPQTR 350 >ref|ZP_01046631.1| enoyl-CoA hydratase [Nitrobacter sp. Nb-311A] gb|EAQ35467.1| enoyl-CoA hydratase [Nitrobacter sp. Nb-311A] Length = 356 Score = 520 bits (1338), Expect = e-145, Method: Compositional matrix adjust. Identities = 255/354 (72%), Positives = 291/354 (82%) Query: 3 DAAAAEGDLIARKEGSAGIIRLNRPKAINAVTLEMFHDIDRALDLFEADGDVAVVVLEGA 62 + AA E DLIAR+EGSAGI+RLNRPKAINAVTL+MF I RALD FEAD V +++LEGA Sbjct: 2 NVAAHEPDLIARREGSAGIVRLNRPKAINAVTLDMFRGIHRALDQFEADPAVGLILLEGA 61 Query: 63 GDRGLCAGGDIRALWESSKVKGDLGKILWRDEYILNARIKKFPKPYVAFMDGIVMGGGVG 122 G+RGLCAGGDIR+LWES+ GDLGK++WR+EY LNARI KFPKPYVAFMDGIVMGGGVG Sbjct: 62 GERGLCAGGDIRSLWESANAGGDLGKVMWREEYTLNARIAKFPKPYVAFMDGIVMGGGVG 121 Query: 123 LSAHSRHRVVTERTKLAMPEVGLGFFPDVGGTYLLSRSPGEIGTYFGLTGTTMNGPDAIY 182 LSAH HRVVTERT+LAMPE GLGFFPDVGGT+LLS +PGEIGT FGLTG MNG DAIY Sbjct: 122 LSAHGAHRVVTERTRLAMPEAGLGFFPDVGGTWLLSHAPGEIGTCFGLTGQNMNGADAIY 181 Query: 183 AKFADAVVPSAKLPALCEALTKVAPGTGSAEIDRLIGGFATGEKSGPVAAMQAKIDGWFA 242 A FAD +VPS KL L EAL + SA + +I GFA G+ VAA++ +ID WFA Sbjct: 182 AGFADVIVPSRKLAVLREALIDLGARADSARVRAVIAGFANGDADSSVAAIRPRIDAWFA 241 Query: 243 RGRMEDVVAALTADGSDMARDTLKTLGEKSPRGMVVTLKLLRLARATATLEECLVREYRA 302 GRM+D++ AL DGS++AR LK L EKSPRG+VVTLKLLRLARA+ATLEECL REYRA Sbjct: 242 HGRMDDILTALQRDGSELARSILKILNEKSPRGLVVTLKLLRLARASATLEECLAREYRA 301 Query: 303 ALEVFASEDFREGVRAAVIDKDRNPTWSPARIEQVTPEMLAPYFAEIGADELKF 356 AL VFAS+DFREGVRAA+IDKDRNP WSP+ IE VTPE++APYFA GADEL F Sbjct: 302 ALAVFASDDFREGVRAAIIDKDRNPKWSPSAIEDVTPEIIAPYFANAGADELVF 355