BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0205.0005.p (311 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771160.1| epoxide hydrolase [Bradyrhizobium japonicum USD... 514 e-144 ref|YP_569843.1| alpha/beta hydrolase fold protein [Rhodopseudom... 475 e-132 ref|YP_004109047.1| alpha/beta hydrolase fold protein [Rhodopseu... 473 e-131 ref|ZP_08629852.1| epoxide hydrolase [Bradyrhizobiaceae bacteriu... 468 e-130 ref|YP_001240076.1| putative epoxide hydrolase [Bradyrhizobium s... 468 e-130 >ref|NP_771160.1| epoxide hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC49785.1| epoxide hydrolase [Bradyrhizobium japonicum USDA 110] Length = 318 Score = 514 bits (1323), Expect = e-144, Method: Compositional matrix adjust. Identities = 246/313 (78%), Positives = 275/313 (87%), Gaps = 2/313 (0%) Query: 1 MSSSRTISANGIDMFVREAGQGPLVVLCHGWPELSYSWRHQIAALAAAGFRVVAPDMRGF 60 MSS+R I ANGID+F+REAGQGPLVVLCHGWPELSYSWRHQI ALAAAGF VVAPDMRG+ Sbjct: 1 MSSTRVIKANGIDLFIREAGQGPLVVLCHGWPELSYSWRHQIPALAAAGFHVVAPDMRGY 60 Query: 61 GRSGAPAEVGAYTIFDTVGDMVALVAALGETRAMIVGHDWGAPVAWHAALFRPDIFTKVA 120 G+S APA+V AY+IFDTVGD+V LV ALGE++AM+VGHDWGAPVAWHAALFRPDIFT VA Sbjct: 61 GQSAAPADVSAYSIFDTVGDIVGLVQALGESKAMVVGHDWGAPVAWHAALFRPDIFTAVA 120 Query: 121 GLSVPPPFRGRGRPLETLRDSGITNFYWQYFQPSGVAEAEFERDIPHTMRLVL-GRGVSD 179 GLSVPPPFRGRG+PL+ LR G+TNFYWQYFQ GVAE E ERD+ TMR+VL GRG++D Sbjct: 121 GLSVPPPFRGRGKPLDLLRQGGVTNFYWQYFQVPGVAEVELERDVARTMRIVLGGRGLAD 180 Query: 180 PS-SMFVDEARGFLGKLPAGMPLPAWLTEADIAEFAEGYRHSGFRGGLNWYRNLDRNWEL 238 PS +MFV E +GFLG A PLP WL+EAD+A F E +R SGFRGGLNWYRNLDRNWEL Sbjct: 181 PSAAMFVQEGKGFLGHATADEPLPDWLSEADLAYFTETFRKSGFRGGLNWYRNLDRNWEL 240 Query: 239 TAPWQDAQIHQPSLFIAGSNDSVITGLIGAKRVADMERVLPNLRQKLIIEGAGHWIQQER 298 TAPWQDAQIHQPSLFIAGS D+VITGLIGAKRV ++ERVLPNL +KLIIEGAGHW+QQER Sbjct: 241 TAPWQDAQIHQPSLFIAGSKDAVITGLIGAKRVNELERVLPNLTRKLIIEGAGHWVQQER 300 Query: 299 PDEVNKALTAFLK 311 PDEVN AL FL+ Sbjct: 301 PDEVNAALVKFLR 313 >ref|YP_569843.1| alpha/beta hydrolase fold protein [Rhodopseudomonas palustris BisB5] gb|ABE39942.1| alpha/beta hydrolase fold [Rhodopseudomonas palustris BisB5] Length = 315 Score = 475 bits (1222), Expect = e-132, Method: Compositional matrix adjust. Identities = 226/312 (72%), Positives = 264/312 (84%), Gaps = 1/312 (0%) Query: 1 MSSSRTISANGIDMFVREAGQGPLVVLCHGWPELSYSWRHQIAALAAAGFRVVAPDMRGF 60 M ++RT++ NGI++F+RE G+GPLV+LCHGWPELSYSWRHQIAALA AGFRVVAPDMRGF Sbjct: 1 METTRTVAVNGIELFLREQGEGPLVLLCHGWPELSYSWRHQIAALAEAGFRVVAPDMRGF 60 Query: 61 GRSGAPAEVGAYTIFDTVGDMVALVAALGETRAMIVGHDWGAPVAWHAALFRPDIFTKVA 120 GRS AP + AY+IFD VGDMVALVA L ETRA I+GHDWGAPVAWHAALFRPD+FT VA Sbjct: 61 GRSSAPQPIEAYSIFDLVGDMVALVAELKETRAAIIGHDWGAPVAWHAALFRPDLFTAVA 120 Query: 121 GLSVPPPFRGRGRPLETLRDSGITNFYWQYFQPSGVAEAEFERDIPHTMRLVLGRGVSDP 180 GLSVPPP+RGRGRPLETLR G+TNFYWQYFQ GVAE+E ERD+ TMR +LG G SDP Sbjct: 121 GLSVPPPWRGRGRPLETLRKGGVTNFYWQYFQAPGVAESELERDVALTMRGILGGGFSDP 180 Query: 181 -SSMFVDEARGFLGKLPAGMPLPAWLTEADIAEFAEGYRHSGFRGGLNWYRNLDRNWELT 239 S+F+ E GF+G++ + LP WL+EA++A F E Y+ SGFRGGLNWYRN+DRNW+LT Sbjct: 181 VRSLFIPEGGGFVGEIASDRSLPDWLSEAELALFVEAYKASGFRGGLNWYRNIDRNWDLT 240 Query: 240 APWQDAQIHQPSLFIAGSNDSVITGLIGAKRVADMERVLPNLRQKLIIEGAGHWIQQERP 299 APWQ AQI QPSLFIAGS D VI+ + K+VA +ERVLPN++QKLII+GAGHWIQQERP Sbjct: 241 APWQGAQIRQPSLFIAGSKDPVISDAMSGKQVAGIERVLPNIQQKLIIDGAGHWIQQERP 300 Query: 300 DEVNKALTAFLK 311 +EVN AL AFLK Sbjct: 301 NEVNAALIAFLK 312 >ref|YP_004109047.1| alpha/beta hydrolase fold protein [Rhodopseudomonas palustris DX-1] gb|ADU44314.1| alpha/beta hydrolase fold protein [Rhodopseudomonas palustris DX-1] Length = 315 Score = 473 bits (1217), Expect = e-131, Method: Compositional matrix adjust. Identities = 226/312 (72%), Positives = 262/312 (83%), Gaps = 1/312 (0%) Query: 1 MSSSRTISANGIDMFVREAGQGPLVVLCHGWPELSYSWRHQIAALAAAGFRVVAPDMRGF 60 M+S+RTI+ NGID+FVRE G GPLV+LCHGWPELSYSWRHQI ALA AG+RV APDMRGF Sbjct: 1 MASTRTIAVNGIDLFVREQGDGPLVILCHGWPELSYSWRHQICALAEAGYRVAAPDMRGF 60 Query: 61 GRSGAPAEVGAYTIFDTVGDMVALVAALGETRAMIVGHDWGAPVAWHAALFRPDIFTKVA 120 GRS AP V AY+IFD VGDMVALVA LGE+RA I+GHDWGAPVAWHAA FRPD+F VA Sbjct: 61 GRSSAPQPVEAYSIFDLVGDMVALVAELGESRAAIIGHDWGAPVAWHAAQFRPDLFAVVA 120 Query: 121 GLSVPPPFRGRGRPLETLRDSGITNFYWQYFQPSGVAEAEFERDIPHTMRLVLGRGVSDP 180 GLSVPPP+RG+GRPL+ LR +G+TNFYWQYFQ GVAEAE ERDI TMR +L G SDP Sbjct: 121 GLSVPPPWRGKGRPLDLLRAAGVTNFYWQYFQTPGVAEAELERDIAATMRGILCGGFSDP 180 Query: 181 S-SMFVDEARGFLGKLPAGMPLPAWLTEADIAEFAEGYRHSGFRGGLNWYRNLDRNWELT 239 + S+FV E RGF+G+ +PLP+WL+EA++A F E Y+ SGFRGGLNWYRN+DRNW+LT Sbjct: 181 ARSLFVPEGRGFIGRSAPTLPLPSWLSEAELAYFIEQYKKSGFRGGLNWYRNIDRNWDLT 240 Query: 240 APWQDAQIHQPSLFIAGSNDSVITGLIGAKRVADMERVLPNLRQKLIIEGAGHWIQQERP 299 APWQ AQIHQPS FIAGSND VI+ + K +A +ERVLPNL++KLII+GAGHWIQQE+P Sbjct: 241 APWQGAQIHQPSAFIAGSNDPVISDKMSGKHLAAIERVLPNLKRKLIIDGAGHWIQQEKP 300 Query: 300 DEVNKALTAFLK 311 EVN AL AFLK Sbjct: 301 AEVNAALVAFLK 312 >ref|ZP_08629852.1| epoxide hydrolase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07495.1| epoxide hydrolase [Bradyrhizobiaceae bacterium SG-6C] Length = 311 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 224/311 (72%), Positives = 261/311 (83%), Gaps = 3/311 (0%) Query: 1 MSSSRTISANGIDMFVREAGQGPLVVLCHGWPELSYSWRHQIAALAAAGFRVVAPDMRGF 60 MSSSR ISANGI++FV E G GPLV+LCHGWPELS+SWRHQ+ ALAAAG+RVVAPDMRG+ Sbjct: 1 MSSSRMISANGIEIFVTEQGAGPLVLLCHGWPELSHSWRHQLPALAAAGYRVVAPDMRGY 60 Query: 61 GRSGAPAEVGAYTIFDTVGDMVALVAALGETRAMIVGHDWGAPVAWHAALFRPDIFTKVA 120 GR+ AP ++ AY+IFD VGDMV LV ALGETRA+I+GHDWGAPVAWHAALFRPD+FT V Sbjct: 61 GRTSAPQDISAYSIFDLVGDMVGLVTALGETRAVIIGHDWGAPVAWHAALFRPDMFTAVG 120 Query: 121 GLSVPPPFRGRGRPLETLRDSGITNFYWQYFQPSGVAEAEFERDIPHTMRLVLGRGVSDP 180 GLSVPPPFRGR RPL+TL SG+TNFYWQYFQ GVAEAEFERD+ TMR V GV Sbjct: 121 GLSVPPPFRGRERPLDTLAKSGVTNFYWQYFQKPGVAEAEFERDVNFTMRAV-SFGVE-- 177 Query: 181 SSMFVDEARGFLGKLPAGMPLPAWLTEADIAEFAEGYRHSGFRGGLNWYRNLDRNWELTA 240 +S+F+ E +GFLG P W++E D+A E YR +GFRGGLNWYRN+DRNWELTA Sbjct: 178 ASLFLKEGQGFLGDPGRERARPVWISEDDLAHVVETYRRTGFRGGLNWYRNIDRNWELTA 237 Query: 241 PWQDAQIHQPSLFIAGSNDSVITGLIGAKRVADMERVLPNLRQKLIIEGAGHWIQQERPD 300 PWQ AQIHQPS+FIAG++D+V+TG+IG KRV +M++VLPNLR+KL+IEGAGHWIQQERPD Sbjct: 238 PWQGAQIHQPSIFIAGADDAVVTGIIGGKRVTEMDKVLPNLRRKLLIEGAGHWIQQERPD 297 Query: 301 EVNKALTAFLK 311 EVN AL FLK Sbjct: 298 EVNAALIEFLK 308 >ref|YP_001240076.1| putative epoxide hydrolase [Bradyrhizobium sp. BTAi1] gb|ABQ36170.1| Putative epoxide hydrolase [Bradyrhizobium sp. BTAi1] Length = 302 Score = 468 bits (1204), Expect = e-130, Method: Compositional matrix adjust. Identities = 224/296 (75%), Positives = 250/296 (84%) Query: 16 VREAGQGPLVVLCHGWPELSYSWRHQIAALAAAGFRVVAPDMRGFGRSGAPAEVGAYTIF 75 +RE G+G LV+LCHGWPELSYSWRHQI ALAAAG+RVVAPDMRGFG S APAE+GAY+IF Sbjct: 1 MREQGEGALVLLCHGWPELSYSWRHQIPALAAAGYRVVAPDMRGFGGSQAPAEIGAYSIF 60 Query: 76 DTVGDMVALVAALGETRAMIVGHDWGAPVAWHAALFRPDIFTKVAGLSVPPPFRGRGRPL 135 D VGDMVALV ALG A+I+GHDWGAPVAWHAALFRPD+F VAGLSVPPPFRGR RPL Sbjct: 61 DIVGDMVALVGALGAQDAVIIGHDWGAPVAWHAALFRPDLFKAVAGLSVPPPFRGRARPL 120 Query: 136 ETLRDSGITNFYWQYFQPSGVAEAEFERDIPHTMRLVLGRGVSDPSSMFVDEARGFLGKL 195 + LR GI FYWQYFQ GVAEAE ERDI TMR +L RG SDP S+FV +GFLG++ Sbjct: 121 DMLRAGGIETFYWQYFQAPGVAEAELERDIALTMRTMLARGFSDPQSLFVTPDKGFLGEV 180 Query: 196 PAGMPLPAWLTEADIAEFAEGYRHSGFRGGLNWYRNLDRNWELTAPWQDAQIHQPSLFIA 255 +PLPAWL+ D+A F + YR SGFRGGLNWYRN+DRNWELTAPWQDAQI QPSLFIA Sbjct: 181 DPDLPLPAWLSADDLAYFVDAYRASGFRGGLNWYRNIDRNWELTAPWQDAQIRQPSLFIA 240 Query: 256 GSNDSVITGLIGAKRVADMERVLPNLRQKLIIEGAGHWIQQERPDEVNKALTAFLK 311 G D+V+TGLIGAKRV DMERVLP+LR+KLI+ GAGHWIQQERPDEVN AL AFL+ Sbjct: 241 GGEDAVVTGLIGAKRVQDMERVLPDLRRKLIVAGAGHWIQQERPDEVNAALIAFLR 296