BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0203.0034.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_771188.1| hypothetical protein bll4548 [Bradyrhizobium ja... 382 e-104 ref|ZP_04943503.1| Alpha/beta hydrolase fold [Burkholderia cenoc... 258 1e-66 ref|YP_623363.1| PHB de-polymerase-like [Burkholderia cenocepaci... 257 2e-66 ref|YP_001779033.1| PHB de-polymerase domain-containing protein ... 257 2e-66 gb|EGD05883.1| PHB de-polymerase domain-containing protein [Burk... 250 2e-64 >ref|NP_771188.1| hypothetical protein bll4548 [Bradyrhizobium japonicum USDA 110] dbj|BAC49813.1| bll4548 [Bradyrhizobium japonicum USDA 110] Length = 371 Score = 382 bits (982), Expect = e-104, Method: Compositional matrix adjust. Identities = 197/342 (57%), Positives = 250/342 (73%), Gaps = 7/342 (2%) Query: 1 LALDVYSQWYPNRAPEPTDSPGEVASLPWTTAHSVALQLPSMCLLDFSRAAEGRPLLVCA 60 LA+D + W EP DS SLPWTT ++VAL+L +M L D ++ G+P LVCA Sbjct: 29 LAMDAWFGWMERGPDEPADS-----SLPWTTPNTVALELATMHLRDCTKDRSGQPALVCA 83 Query: 61 PYALHRAQIADLAPGYSLMEALQKAGVARLYLTDWRSAAPEMRYFSIDTFLSDLNVAVDT 120 PYALHRA IAD APG+S++++LQ G+ R+YLTDWRSA P+MRY SID++L+DLNVA+D Sbjct: 84 PYALHRALIADFAPGHSVVQSLQNGGIDRVYLTDWRSATPDMRYLSIDSYLADLNVAIDE 143 Query: 121 IGPPVDLAGLCQGGWLSLLYAARFPGKVRRLVLAGSPVDVSVPSELSRMVASLPQQGFEA 180 IG PVDL GLCQGGWLSLLYAARFP KVRRLVL G+PVD+S+ S LSR+ + P+ ++ Sbjct: 144 IGVPVDLVGLCQGGWLSLLYAARFPAKVRRLVLVGAPVDLSIDSSLSRLARNAPEMVYDQ 203 Query: 181 LVRQGDGIVSGKQMLKVWSIPFSLLDVEAALQRRLDGTSEDARALLDRFTHWDSDPLDLP 240 LV +G G VSG++ML+ WS S D+ AALQ L + E+ ALL RF W+ + LDLP Sbjct: 204 LVARGGGNVSGEEMLRFWSRTPSREDIAAALQVGL--SDEEGGALLARFDQWNVETLDLP 261 Query: 241 GTYYLEVTDWIFRQNRIAAGHFVALGRKIDLAAVKLPVFLLAAENDIVVPPDQAFATMRL 300 GTYYLE+ + IFR+N+IA G+FVALGR IDL VK P+FLLA +D VVP QA AT L Sbjct: 262 GTYYLEIVNRIFRENQIAGGNFVALGRAIDLKDVKAPIFLLAGLDDEVVPVTQALATAGL 321 Query: 301 LGTRPAWLQRETEPCSHLGLFMGRRALAGSWRRIARWLQSDL 342 +GT PA++ +EP +HLGLFMG R A +W RIA+WL+ DL Sbjct: 322 VGTPPAFIAAASEPSNHLGLFMGARTHAHAWPRIAQWLRDDL 363 >ref|ZP_04943503.1| Alpha/beta hydrolase fold [Burkholderia cenocepacia PC184] gb|EAY66674.1| Alpha/beta hydrolase fold [Burkholderia cenocepacia PC184] Length = 435 Score = 258 bits (659), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 144/309 (46%), Positives = 186/309 (60%), Gaps = 11/309 (3%) Query: 37 LQLPSMCLLDFSRA-AEGRPLLVCAPYALHRAQIADLAPGYSLMEALQKAGVARLYLTDW 95 L+L +M D+S A A G P +V APYA H A IAD PG SLM+ L++ GV RLYLTDW Sbjct: 127 LELRTMTFRDYSAAPAHGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVTRLYLTDW 186 Query: 96 RSAAPEMRYFSIDTFLSDLNVAVDTIGPPVDLAGLCQGGWLSLLYAARFPGKVRRLVLAG 155 RSA +M+ ID +L++LNV VD +G V+L GLCQGGW+S +YAARFP KV LVLAG Sbjct: 187 RSATEDMKDLEIDQYLAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAG 246 Query: 156 SPVDVSV-PSELSRMVASLPQQGFEALVRQGDGIVSGKQMLKVWSIPF---SLLDVEAAL 211 SP+D + +MV + P +E LV G G++ G+ ML+ W L L Sbjct: 247 SPIDTDAGDGPIKQMVHTYPTSFYEELVAMGGGLMRGRFMLRGWKNMHPDQHYLAEHVDL 306 Query: 212 QRRLDGTSEDARALLDR--FTHWDSDPLDLPGTYYLEVTDWIFRQNRIAAGHFVALGRKI 269 LD D L R F +W P+DLPG +YL+ IF++NR+A G FVALGR + Sbjct: 307 YEHLD----DPEYLRKREVFANWYESPIDLPGRWYLQAIVQIFKENRLAKGTFVALGRTL 362 Query: 270 DLAAVKLPVFLLAAENDIVVPPDQAFATMRLLGTRPAWLQRETEPCSHLGLFMGRRALAG 329 DL V P++LLA E D + P+Q F LLGT + ++ P H+GLFMG R LA Sbjct: 363 DLKDVACPIYLLAGEADDITTPEQVFGARDLLGTPASRIESRLVPGGHIGLFMGTRTLAT 422 Query: 330 SWRRIARWL 338 W IARW+ Sbjct: 423 VWPDIARWI 431 >ref|YP_623363.1| PHB de-polymerase-like [Burkholderia cenocepacia AU 1054] ref|YP_838497.1| PHB de-polymerase domain-containing protein [Burkholderia cenocepacia HI2424] gb|ABF78390.1| PHB de-polymerase-like protein [Burkholderia cenocepacia AU 1054] gb|ABK11604.1| PHB de-polymerase domain protein [Burkholderia cenocepacia HI2424] Length = 373 Score = 257 bits (657), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 141/308 (45%), Positives = 189/308 (61%), Gaps = 9/308 (2%) Query: 37 LQLPSMCLLDFSRA-AEGRPLLVCAPYALHRAQIADLAPGYSLMEALQKAGVARLYLTDW 95 L+L +M D+S A A G P +V APYA H A IAD PG SLM+ L++ GV R+YLTDW Sbjct: 65 LELRTMTFRDYSAAPAHGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVTRVYLTDW 124 Query: 96 RSAAPEMRYFSIDTFLSDLNVAVDTIGPPVDLAGLCQGGWLSLLYAARFPGKVRRLVLAG 155 RSA +M+ ID +L++LNV VD +G V+L GLCQGGW+S +YAARFP KV LVLAG Sbjct: 125 RSATEDMKDLEIDQYLAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAG 184 Query: 156 SPVDVSV-PSELSRMVASLPQQGFEALVRQGDGIVSGKQMLKVWSIPFSLLDVEAALQRR 214 SP+D + RMV + P ++ LV G G++ G+ ML+ W ++ + L Sbjct: 185 SPIDTDAGDGPIKRMVHTYPTAFYDELVAMGGGLMRGRFMLRGWK---NMHPDQHYLAEH 241 Query: 215 LDGTS--EDARALLDR--FTHWDSDPLDLPGTYYLEVTDWIFRQNRIAAGHFVALGRKID 270 +D +D L R F +W P+DLPG +YL+ IF++NR+A G FVALGR +D Sbjct: 242 IDLYEHLDDPEYLRKREVFANWYESPIDLPGRWYLQAIVQIFKENRLAKGTFVALGRTLD 301 Query: 271 LAAVKLPVFLLAAENDIVVPPDQAFATMRLLGTRPAWLQRETEPCSHLGLFMGRRALAGS 330 L V PV+LLA E D + P+Q F LLGT + ++ P H+G+FMG R LA Sbjct: 302 LKDVACPVYLLAGEADDITTPEQVFGARDLLGTPASRIESRLVPGGHIGMFMGTRTLATV 361 Query: 331 WRRIARWL 338 W IARW+ Sbjct: 362 WPDIARWI 369 >ref|YP_001779033.1| PHB de-polymerase domain-containing protein [Burkholderia cenocepacia MC0-3] gb|ACA94543.1| PHB de-polymerase domain protein [Burkholderia cenocepacia MC0-3] Length = 373 Score = 257 bits (657), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 141/308 (45%), Positives = 189/308 (61%), Gaps = 9/308 (2%) Query: 37 LQLPSMCLLDFSRA-AEGRPLLVCAPYALHRAQIADLAPGYSLMEALQKAGVARLYLTDW 95 L+L +M D+S A A G P +V APYA H A IAD PG SLM+ L++ GV R+YLTDW Sbjct: 65 LELRTMTFRDYSAAPAHGLPTIVDAPYAGHSAMIADYQPGQSLMQTLREHGVTRVYLTDW 124 Query: 96 RSAAPEMRYFSIDTFLSDLNVAVDTIGPPVDLAGLCQGGWLSLLYAARFPGKVRRLVLAG 155 RSA +M+ ID +L++LNV VD +G V+L GLCQGGW+S +YAARFP KV LVLAG Sbjct: 125 RSATEDMKDLEIDQYLAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAG 184 Query: 156 SPVDVSV-PSELSRMVASLPQQGFEALVRQGDGIVSGKQMLKVWSIPFSLLDVEAALQRR 214 SP+D + RMV + P ++ LV G G++ G+ ML+ W ++ + L Sbjct: 185 SPIDTDAGDGPIKRMVHTYPTAFYDELVAMGGGLMRGRFMLRGWK---NMHPDQHYLAEH 241 Query: 215 LDGTS--EDARALLDR--FTHWDSDPLDLPGTYYLEVTDWIFRQNRIAAGHFVALGRKID 270 +D +D L R F +W P+DLPG +YL+ IF++NR+A G FVALGR +D Sbjct: 242 IDLYEHLDDPEYLRKREVFANWYESPIDLPGRWYLQAIVQIFKENRLAKGTFVALGRTLD 301 Query: 271 LAAVKLPVFLLAAENDIVVPPDQAFATMRLLGTRPAWLQRETEPCSHLGLFMGRRALAGS 330 L V PV+LLA E D + P+Q F LLGT + ++ P H+G+FMG R LA Sbjct: 302 LKDVACPVYLLAGEADDITTPEQVFGARDLLGTPASRIESRLVPGGHIGMFMGTRTLATV 361 Query: 331 WRRIARWL 338 W IARW+ Sbjct: 362 WPDIARWI 369 >gb|EGD05883.1| PHB de-polymerase domain-containing protein [Burkholderia sp. TJI49] Length = 296 Score = 250 bits (639), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 133/293 (45%), Positives = 177/293 (60%), Gaps = 8/293 (2%) Query: 51 AEGRPLLVCAPYALHRAQIADLAPGYSLMEALQKAGVARLYLTDWRSAAPEMRYFSIDTF 110 +G P +V APYA H A IAD PG SL E L+ GV RLYLTDWRSA +M+ F ID + Sbjct: 3 GQGLPTIVDAPYAGHSAMIADYQPGQSLTETLRGNGVTRLYLTDWRSATDDMKDFEIDQY 62 Query: 111 LSDLNVAVDTIGPPVDLAGLCQGGWLSLLYAARFPGKVRRLVLAGSPVDVSV-PSELSRM 169 L++LNV VD +G V+L GLCQGGW+S +YAARFP KV LVLAG+P+D + RM Sbjct: 63 LAELNVCVDELGGRVNLVGLCQGGWMSAMYAARFPHKVASLVLAGAPIDTDAGDGPIRRM 122 Query: 170 VASLPQQGFEALVRQGDGIVSGKQMLKVWSIPFSLLDVEAALQRRLDGTSE----DARAL 225 + P +E LV G G++ G+ ML+ W ++ E +L +D + D Sbjct: 123 AHAYPLSFYEELVAMGGGLMRGRFMLRGWK---NMHPDEHSLSEHVDLYAHLDEPDYLRK 179 Query: 226 LDRFTHWDSDPLDLPGTYYLEVTDWIFRQNRIAAGHFVALGRKIDLAAVKLPVFLLAAEN 285 + F W +P+DLPG +YL+ + IF++NR+A G FVALGR +DL V+ PV+LLA E Sbjct: 180 REAFASWYENPIDLPGRWYLQAIEQIFKENRLAKGAFVALGRTLDLKNVRCPVYLLAGEA 239 Query: 286 DIVVPPDQAFATMRLLGTRPAWLQRETEPCSHLGLFMGRRALAGSWRRIARWL 338 D + P+Q F +GT A + P H+GLFMG R L W IARW+ Sbjct: 240 DDITTPEQVFNARDCVGTPAAQVTSRLVPGGHIGLFMGSRTLKAVWPEIARWI 292