BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0086.p (406 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769280.1| major facilitator superfamily transporter [Brad... 575 e-162 ref|YP_002280694.1| major facilitator superfamily protein [Rhizo... 509 e-142 ref|ZP_07025784.1| major facilitator superfamily MFS_1 [Afipia s... 498 e-139 ref|YP_001668104.1| major facilitator transporter [Pseudomonas p... 495 e-138 ref|YP_002290254.1| major facilitator superfamily transporter [O... 489 e-136 >ref|NP_769280.1| major facilitator superfamily transporter [Bradyrhizobium japonicum USDA 110] dbj|BAC47905.1| major facilitator superfamily transporter [Bradyrhizobium japonicum USDA 110] Length = 402 Score = 575 bits (1482), Expect = e-162, Method: Compositional matrix adjust. Identities = 306/390 (78%), Positives = 341/390 (87%), Gaps = 2/390 (0%) Query: 5 PNSPSPPARIPTGIWALGFVSMLMDISSEMIHALLPVYLVTVLGTSMVTVGFIEGIAEAT 64 PN+ A +P GIW LGFVSMLMD+SSEMIHALLPVYLVTVLG S +TVGFIEGIAEAT Sbjct: 6 PNAAR--AGLPRGIWVLGFVSMLMDVSSEMIHALLPVYLVTVLGASTLTVGFIEGIAEAT 63 Query: 65 ASITKIFSGALSDWLGKRKLLAAIGYGIAAFTKPVFPLAPTVDWLIAARFIDRVGKGIRG 124 ASITKIFSGALSDWLG+RKLLAA+GYG+AA TKP+FPLAP+V+WL+AARFIDRVGKGIRG Sbjct: 64 ASITKIFSGALSDWLGRRKLLAALGYGLAAVTKPLFPLAPSVEWLVAARFIDRVGKGIRG 123 Query: 125 APRDALVADIAPAELRGASFGLRQSLDTVGAFVGPLLAIALMWVSADNFHAVFWLAVVPA 184 APRDAL+ADIAP LRGASFGLRQSLDT+GAFVGPL AI LMW +ADNF AVFW+AV+PA Sbjct: 124 APRDALIADIAPVGLRGASFGLRQSLDTIGAFVGPLAAIGLMWWTADNFTAVFWVAVLPA 183 Query: 185 FLSLAVIVVAVEEPRRHADDGGTKNPLSLAAAKQLGESYWRVVGIGVVFTLARFSEAFLI 244 FLS +I AV EP KNPL+LAA +QLG YWRVV +G+VFTLARFSEAFLI Sbjct: 184 FLSFGLIAFAVSEPEPDPSRESAKNPLNLAAMRQLGAVYWRVVAVGIVFTLARFSEAFLI 243 Query: 245 LRAQNVGLGAMWIPAVLVLMNIVYALSAYPAGVLSDRINRTGLLALGLVCLAAADLALAT 304 LRAQN+GL AMW+PAVLVLMNIVYALSAYPAGVLSDRINRT LLALGLV LA ADLALA Sbjct: 244 LRAQNIGLNAMWVPAVLVLMNIVYALSAYPAGVLSDRINRTSLLALGLVFLAGADLALAL 303 Query: 305 LANLGGLALGVILWGLHMGLTQGLFATLIADASPASLRGTAYGYFNLSTGVAMLGSSVIA 364 L NLGGLALGV+LWGLHMGLTQGL + L+ADA+P SLRGTA+GYFNL TG+A+L +SVIA Sbjct: 304 LPNLGGLALGVVLWGLHMGLTQGLLSALVADAAPPSLRGTAFGYFNLFTGLALLAASVIA 363 Query: 365 GALWDAFGPAATFLSGLAFTLLSLAGLLAI 394 GALWDA+GPA TFL+GL F L+SL GLLA+ Sbjct: 364 GALWDAYGPAGTFLAGLGFALVSLVGLLAV 393 >ref|YP_002280694.1| major facilitator superfamily protein [Rhizobium leguminosarum bv. trifolii WSM2304] gb|ACI54468.1| major facilitator superfamily MFS_1 [Rhizobium leguminosarum bv. trifolii WSM2304] Length = 409 Score = 509 bits (1312), Expect = e-142, Method: Compositional matrix adjust. Identities = 260/389 (66%), Positives = 311/389 (79%) Query: 8 PSPPARIPTGIWALGFVSMLMDISSEMIHALLPVYLVTVLGTSMVTVGFIEGIAEATASI 67 P+ RIP GIWALGFVSMLMDISSEMIHALLPVY+V+VLG SM VG IEGIAEATASI Sbjct: 13 PATYRRIPAGIWALGFVSMLMDISSEMIHALLPVYMVSVLGISMFAVGVIEGIAEATASI 72 Query: 68 TKIFSGALSDWLGKRKLLAAIGYGIAAFTKPVFPLAPTVDWLIAARFIDRVGKGIRGAPR 127 TK+FSGALSDWLG+RK LAA+GYG+AA TKP+FPLA ++DWLIAARF+DRVGKGIRGAPR Sbjct: 73 TKVFSGALSDWLGRRKFLAALGYGLAAVTKPIFPLASSLDWLIAARFVDRVGKGIRGAPR 132 Query: 128 DALVADIAPAELRGASFGLRQSLDTVGAFVGPLLAIALMWVSADNFHAVFWLAVVPAFLS 187 DALVADIAP ELRGASFGLRQSLDTVGAFVGPLLAI LMW++AD+F V W+AV+PAFLS Sbjct: 133 DALVADIAPPELRGASFGLRQSLDTVGAFVGPLLAIGLMWLTADHFQRVLWIAVLPAFLS 192 Query: 188 LAVIVVAVEEPRRHADDGGTKNPLSLAAAKQLGESYWRVVGIGVVFTLARFSEAFLILRA 247 + V++ V+EP R + + PL +LG SYW VV + FTLARFS+AFLIL+A Sbjct: 193 VGVLLFVVKEPERPREFRHVRMPLHRDELGRLGRSYWWVVAVAAAFTLARFSDAFLILKA 252 Query: 248 QNVGLGAMWIPAVLVLMNIVYALSAYPAGVLSDRINRTGLLALGLVCLAAADLALATLAN 307 Q++GL +P LVLM++ Y+LSAYPAG+LSD+++R +LA+GLV L ADL LA + Sbjct: 253 QSIGLPIALVPLALVLMSLAYSLSAYPAGMLSDKMDRFTILAIGLVLLVCADLTLAFAQS 312 Query: 308 LGGLALGVILWGLHMGLTQGLFATLIADASPASLRGTAYGYFNLSTGVAMLGSSVIAGAL 367 + G LGV+LWGLHMG TQGL +IAD SPA LRGTA+G FNL TG+A+L +SVIAG L Sbjct: 313 VIGAGLGVLLWGLHMGFTQGLLTKVIADTSPAELRGTAFGMFNLITGLALLLASVIAGTL 372 Query: 368 WDAFGPAATFLSGLAFTLLSLAGLLAIDT 396 WD GP TFL+G F +L++ GLL + T Sbjct: 373 WDLAGPRGTFLAGAGFAMLTMIGLLVVRT 401 >ref|ZP_07025784.1| major facilitator superfamily MFS_1 [Afipia sp. 1NLS2] gb|EFI52926.1| major facilitator superfamily MFS_1 [Afipia sp. 1NLS2] Length = 412 Score = 498 bits (1282), Expect = e-139, Method: Compositional matrix adjust. Identities = 258/393 (65%), Positives = 312/393 (79%), Gaps = 5/393 (1%) Query: 6 NSPSPPA----RIPTGIWALGFVSMLMDISSEMIHALLPVYLVTVLGTSMVTVGFIEGIA 61 N PP+ +P GIW LGFVSM MDISSEMIHALLPVYLVTVL S +TVG IEG+A Sbjct: 3 NHVLPPSIGLRALPRGIWTLGFVSMFMDISSEMIHALLPVYLVTVLRASTLTVGLIEGLA 62 Query: 62 EATASITKIFSGALSDWLGKRKLLAAIGYGIAAFTKPVFPLAPTVDWLIAARFIDRVGKG 121 EATA ITK+FSGALSDWLG+RKLLAAIGYG++A TKP+FPLA T++W++AARF+DRVGKG Sbjct: 63 EATAMITKVFSGALSDWLGRRKLLAAIGYGLSAVTKPIFPLATTIEWVVAARFLDRVGKG 122 Query: 122 IRGAPRDALVADIAPAELRGASFGLRQSLDTVGAFVGPLLAIALMWVSADNFHAVFWLAV 181 +RGAPRDALVAD+APA LRGASFGLRQSLDT+GAF+GPL A+ LM ++ DN+ VFW+AV Sbjct: 123 VRGAPRDALVADLAPARLRGASFGLRQSLDTIGAFLGPLAALGLMLLTRDNYAMVFWIAV 182 Query: 182 VPAFLSLAVIVVAVEEPRRHADDGGTKNPLSLAAAKQLGESYWRVVGIGVVFTLARFSEA 241 VPAF +LA++V V EP R A P + A +L +W VVG+ VV TLARFSEA Sbjct: 183 VPAFFALALMVFGVAEPERAAATLEKPKP-RFSDAWRLSRPFWAVVGVAVVLTLARFSEA 241 Query: 242 FLILRAQNVGLGAMWIPAVLVLMNIVYALSAYPAGVLSDRINRTGLLALGLVCLAAADLA 301 FL+LRAQNVGL +PAV+V+MNIVYALSAYPAGVLSDR R G+L +G+ L ADL Sbjct: 242 FLVLRAQNVGLAIAIVPAVMVVMNIVYALSAYPAGVLSDRFGRQGVLIVGVSTLVFADLI 301 Query: 302 LATLANLGGLALGVILWGLHMGLTQGLFATLIADASPASLRGTAYGYFNLSTGVAMLGSS 361 LA ++ L +GV+ WGLHMGLTQGL ATL+AD +PA LRGTA+G FNL++G+AML +S Sbjct: 302 LAFTTSVPVLLVGVVFWGLHMGLTQGLLATLVADTAPAELRGTAFGVFNLASGIAMLIAS 361 Query: 362 VIAGALWDAFGPAATFLSGLAFTLLSLAGLLAI 394 V+AGALWD +GP ATFL+G A T ++L G LA+ Sbjct: 362 VVAGALWDIYGPVATFLAGAALTAMALIGRLAL 394 >ref|YP_001668104.1| major facilitator transporter [Pseudomonas putida GB-1] gb|ABY97768.1| major facilitator superfamily MFS_1 [Pseudomonas putida GB-1] Length = 401 Score = 495 bits (1275), Expect = e-138, Method: Compositional matrix adjust. Identities = 253/398 (63%), Positives = 312/398 (78%), Gaps = 4/398 (1%) Query: 6 NSPSPPAR----IPTGIWALGFVSMLMDISSEMIHALLPVYLVTVLGTSMVTVGFIEGIA 61 N S PAR IP +WALGFVSM MDISSEMIHALLP+Y+V+VLG S+V VGFIEGIA Sbjct: 4 NEDSSPARGLTKIPRSVWALGFVSMFMDISSEMIHALLPLYMVSVLGASVVAVGFIEGIA 63 Query: 62 EATASITKIFSGALSDWLGKRKLLAAIGYGIAAFTKPVFPLAPTVDWLIAARFIDRVGKG 121 EATASITK+FSGALSD LGKRKLL +GYG+ A TKPVFP+A ++WL AARF+DRVGKG Sbjct: 64 EATASITKVFSGALSDRLGKRKLLTVLGYGLGALTKPVFPMASGLEWLTAARFVDRVGKG 123 Query: 122 IRGAPRDALVADIAPAELRGASFGLRQSLDTVGAFVGPLLAIALMWVSADNFHAVFWLAV 181 IRGAPRDALVAD+ PAELRGA+FGLRQ+LDTVGAF+GPLLAI LMW++A +F VFW+AV Sbjct: 124 IRGAPRDALVADVTPAELRGAAFGLRQALDTVGAFLGPLLAIVLMWLTASHFQTVFWVAV 183 Query: 182 VPAFLSLAVIVVAVEEPRRHADDGGTKNPLSLAAAKQLGESYWRVVGIGVVFTLARFSEA 241 +PAF+++ +++ V EP A ++PL+L +LG YWR++G+ +FTLARFSEA Sbjct: 184 IPAFVAVYILIAFVREPETPATGRPVRSPLALHELGRLGPPYWRLIGLATLFTLARFSEA 243 Query: 242 FLILRAQNVGLGAMWIPAVLVLMNIVYALSAYPAGVLSDRINRTGLLALGLVCLAAADLA 301 FL+LRAQ++GL +W PAVLVLM + Y+LSAYPAGVLSDR+ R G+L +GL L ADL Sbjct: 244 FLLLRAQDMGLAPLWAPAVLVLMALAYSLSAYPAGVLSDRVGRRGVLMVGLGLLITADLL 303 Query: 302 LATLANLGGLALGVILWGLHMGLTQGLFATLIADASPASLRGTAYGYFNLSTGVAMLGSS 361 LA L GLALGV WGLH+G +QG+FA +IAD++PA+LRGTA+G FNL TG+A+L +S Sbjct: 304 LALLPGWAGLALGVAAWGLHLGFSQGIFAAMIADSAPANLRGTAFGLFNLLTGMALLAAS 363 Query: 362 VIAGALWDAFGPAATFLSGLAFTLLSLAGLLAIDTSGK 399 V+AG LWD G ATFL G F +LAGL + +G Sbjct: 364 VVAGLLWDGVGFQATFLVGAGFAGATLAGLTLMKVTGS 401 >ref|YP_002290254.1| major facilitator superfamily transporter [Oligotropha carboxidovorans OM5] ref|YP_004631794.1| MFS transporter protein [Oligotropha carboxidovorans OM5] gb|ACI94389.1| major facilitator superfamily transporter [Oligotropha carboxidovorans OM5] gb|AEI01977.1| MFS transporter protein [Oligotropha carboxidovorans OM4] gb|AEI05553.1| MFS transporter protein [Oligotropha carboxidovorans OM5] Length = 411 Score = 489 bits (1260), Expect = e-136, Method: Compositional matrix adjust. Identities = 254/396 (64%), Positives = 308/396 (77%), Gaps = 5/396 (1%) Query: 4 EPNSPSPPAR-----IPTGIWALGFVSMLMDISSEMIHALLPVYLVTVLGTSMVTVGFIE 58 P++ + P R IP GIWALGFVSMLMDISSEMIHALLPVYL+TVLGTS + VG IE Sbjct: 9 SPSAAALPRRTRLRDIPGGIWALGFVSMLMDISSEMIHALLPVYLMTVLGTSALAVGVIE 68 Query: 59 GIAEATASITKIFSGALSDWLGKRKLLAAIGYGIAAFTKPVFPLAPTVDWLIAARFIDRV 118 G+AEATASI K+FSGALSD LGKRK LAA GYG+AA TKP+FPLA +V WL+AARFIDR+ Sbjct: 69 GVAEATASIMKVFSGALSDRLGKRKWLAAFGYGLAALTKPIFPLASSVGWLVAARFIDRI 128 Query: 119 GKGIRGAPRDALVADIAPAELRGASFGLRQSLDTVGAFVGPLLAIALMWVSADNFHAVFW 178 GKGIRGAPRDALVADIAPA LRGASFGLRQSLDT+GAF+GP LAI LMW++A++F VFW Sbjct: 129 GKGIRGAPRDALVADIAPAHLRGASFGLRQSLDTIGAFLGPGLAIGLMWLTANHFQTVFW 188 Query: 179 LAVVPAFLSLAVIVVAVEEPRRHADDGGTKNPLSLAAAKQLGESYWRVVGIGVVFTLARF 238 +AV+PA LS+ +I+ AV +P R +PLS+ A ++LG +YW V I VFTLARF Sbjct: 189 IAVIPAVLSVLLIIFAVHDPERDGTARAVHSPLSVEAMRRLGAAYWITVAISTVFTLARF 248 Query: 239 SEAFLILRAQNVGLGAMWIPAVLVLMNIVYALSAYPAGVLSDRINRTGLLALGLVCLAAA 298 SEAFLIL+AQ VG+ +P VLVLMN+VYAL++YP GVLSDR++R LLA GLV L A Sbjct: 249 SEAFLILKAQAVGMPIFLVPLVLVLMNVVYALTSYPIGVLSDRMSRVDLLAFGLVVLVVA 308 Query: 299 DLALATLANLGGLALGVILWGLHMGLTQGLFATLIADASPASLRGTAYGYFNLSTGVAML 358 D+ L +L G+A GV LWGLHMG TQG+ A L+AD +PA LRGTA+G NL GV +L Sbjct: 309 DIVLGFTTSLIGVAFGVALWGLHMGCTQGVVAALVADTAPAELRGTAFGVVNLLGGVMLL 368 Query: 359 GSSVIAGALWDAFGPAATFLSGLAFTLLSLAGLLAI 394 +SV+AG LWD G TFL+G F++ +LAG++ + Sbjct: 369 AASVLAGLLWDQTGAQGTFLAGAGFSVAALAGIVLV 404