BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0012.p (410 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774671.1| DNA ligase [Bradyrhizobium japonicum USDA 110] ... 505 e-141 ref|ZP_01046721.1| DNA ligase-like protein [Nitrobacter sp. Nb-3... 484 e-134 ref|YP_679427.1| ATP-dependent DNA ligase [Cytophaga hutchinsoni... 278 1e-72 ref|YP_003019953.1| DNA ligase D [Geobacter sp. M21] >gi|2517736... 274 3e-71 ref|YP_002136955.1| DNA ligase D [Geobacter bemidjiensis Bem] >g... 271 2e-70 >ref|NP_774671.1| DNA ligase [Bradyrhizobium japonicum USDA 110] dbj|BAC53296.1| blr8031 [Bradyrhizobium japonicum USDA 110] Length = 316 Score = 505 bits (1300), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/293 (83%), Positives = 261/293 (89%) Query: 1 MSRTSTLPKRLQPMLATLTDAPFDDPGWVFEDKYDGFRMIAKIRRGKVALYSRNGKIISR 60 M RTS+LPKRLQPMLATLTD PFDDP WVFEDKYDGFRMIA+IR G+ ALYSRNGK+ISR Sbjct: 1 MGRTSSLPKRLQPMLATLTDDPFDDPAWVFEDKYDGFRMIAEIRDGRAALYSRNGKLISR 60 Query: 61 SYIEVAKALEGVKGDAVIDGELVAIGKDGVSHFQLLQNALRHEAKLLYCAFDLMFADGED 120 SY EVA ALE VKGDAVIDGELVAIGKDGVSHFQLLQNALRHEAKLLY AFDLMFA+GED Sbjct: 61 SYTEVAAALEMVKGDAVIDGELVAIGKDGVSHFQLLQNALRHEAKLLYFAFDLMFANGED 120 Query: 121 LRALPLLERKKRLKAILPRHKLVAFSNHRKGKGTKFFADAERRHLEGIMAKRADSPYASG 180 LR PLLERK+RLK ILPRH L+ FS HRKGKG KFFA+AER LEGIMAKR D YASG Sbjct: 121 LRKRPLLERKERLKTILPRHGLIGFSRHRKGKGRKFFAEAERGGLEGIMAKRIDGAYASG 180 Query: 181 RRTADWLKVKTAQRQEVVIAGFTAPRRTRPFFGALVLAVREGDAWRYIGHVGTGFSHQVL 240 RT DWLK+KTA+RQEVVI GFTAPRRTRPFFGALVLAVRE ++WRYIGHVGTGFSHQ L Sbjct: 181 SRTPDWLKIKTAKRQEVVIVGFTAPRRTRPFFGALVLAVREKESWRYIGHVGTGFSHQTL 240 Query: 241 EELHGRLMKLKTAKSPFPAKVKDEQVTTWVRPALVAEVKFAEWTSKGELRQPV 293 ELH RL +L+ AKSPFPAKVKDE VTTWVRP LVAEVKFAEWT+KGELRQPV Sbjct: 241 AELHSRLTRLQVAKSPFPAKVKDETVTTWVRPFLVAEVKFAEWTTKGELRQPV 293 >ref|ZP_01046721.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] gb|EAQ35274.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] Length = 315 Score = 484 bits (1246), Expect = e-134, Method: Compositional matrix adjust. Identities = 232/293 (79%), Positives = 258/293 (88%) Query: 1 MSRTSTLPKRLQPMLATLTDAPFDDPGWVFEDKYDGFRMIAKIRRGKVALYSRNGKIISR 60 M+R STLPK+LQPMLATLTD PFDD GW+FEDKYDGFRM+AKI GKVALYSRNGKIIS Sbjct: 1 MARVSTLPKQLQPMLATLTDGPFDDKGWIFEDKYDGFRMVAKIASGKVALYSRNGKIISH 60 Query: 61 SYIEVAKALEGVKGDAVIDGELVAIGKDGVSHFQLLQNALRHEAKLLYCAFDLMFADGED 120 SYIEVA+ALEGVKGDAVIDGELVA+ +GVSHFQLLQNALR +A+L Y AFDLMF DG+D Sbjct: 61 SYIEVARALEGVKGDAVIDGELVALDDEGVSHFQLLQNALRRQARLRYFAFDLMFQDGDD 120 Query: 121 LRALPLLERKKRLKAILPRHKLVAFSNHRKGKGTKFFADAERRHLEGIMAKRADSPYASG 180 LR LPL ERKKRLKAILP+HKL+AFS RK GTKFF +AER+ LEGIMAKRADS Y SG Sbjct: 121 LRGLPLTERKKRLKAILPKHKLIAFSRDRKTFGTKFFEEAERKGLEGIMAKRADSKYQSG 180 Query: 181 RRTADWLKVKTAQRQEVVIAGFTAPRRTRPFFGALVLAVREGDAWRYIGHVGTGFSHQVL 240 RT +WLK+KT++RQEVVIAGFTAPR+TRPFFGAL LA+R+GD WRYIGHVGTGFSH L Sbjct: 181 ARTDNWLKIKTSKRQEVVIAGFTAPRKTRPFFGALTLALRDGDNWRYIGHVGTGFSHDTL 240 Query: 241 EELHGRLMKLKTAKSPFPAKVKDEQVTTWVRPALVAEVKFAEWTSKGELRQPV 293 +ELHG+L+KLKTAKSPF KVKDE VTTWV+P LVAEVKF EWTS GE+R PV Sbjct: 241 KELHGKLIKLKTAKSPFGKKVKDEAVTTWVKPQLVAEVKFTEWTSSGEMRHPV 293 >ref|YP_679427.1| ATP-dependent DNA ligase [Cytophaga hutchinsonii ATCC 33406] gb|ABG60085.1| ATP-dependent DNA ligase LigD phosphoesterase module / ATP-dependent DNA ligase LigD polymerase module [Cytophaga hutchinsonii ATCC 33406] Length = 896 Score = 278 bits (710), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 131/288 (45%), Positives = 183/288 (63%), Gaps = 1/288 (0%) Query: 7 LPKRLQPMLATLTDAPFDDPGWVFEDKYDGFRMIAKIRRGKVALYSRNGKIISRSYIEVA 66 +P ++PMLA L DAPFD P W+FE K+DG+R IA+I +GKV LYSRN ++ Y + Sbjct: 236 MPHEVKPMLAKLKDAPFDSPEWIFEIKWDGYRAIAEIDQGKVELYSRNLISFTKKYKPIV 295 Query: 67 KALEGVKGDAVIDGELVAIGKDGVSHFQLLQNALRHEA-KLLYCAFDLMFADGEDLRALP 125 ++L+ + V+DGE++ + DG S FQ+LQ + L YC FD++F DG DLR LP Sbjct: 296 ESLQSFGHNVVLDGEVIVLNADGTSSFQMLQQYDETPSPDLCYCVFDILFLDGRDLRDLP 355 Query: 126 LLERKKRLKAILPRHKLVAFSNHRKGKGTKFFADAERRHLEGIMAKRADSPYASGRRTAD 185 LLERK LK ++P + +S+H + +G +FF A++ LEGIMAKRADS Y G R+ Sbjct: 356 LLERKAILKKVIPDLPNLKYSDHVEKEGIRFFELAKKNKLEGIMAKRADSIYRLGSRSDQ 415 Query: 186 WLKVKTAQRQEVVIAGFTAPRRTRPFFGALVLAVREGDAWRYIGHVGTGFSHQVLEELHG 245 W+K+K + QE +I GFTAPR +R FGAL+L E YIGH G GF+ +L Sbjct: 416 WIKLKIVKSQEAIICGFTAPRNSRKDFGALILGAYENGRLVYIGHTGGGFTMALLASTLK 475 Query: 246 RLMKLKTAKSPFPAKVKDEQVTTWVRPALVAEVKFAEWTSKGELRQPV 293 + L +SPF K+K + TW++P LV + F+EWTS G +RQP+ Sbjct: 476 LMKPLIQKESPFDVKIKTNEKVTWIKPKLVCAIAFSEWTSDGHMRQPI 523 >ref|YP_003019953.1| DNA ligase D [Geobacter sp. M21] gb|ACT16195.1| DNA ligase D [Geobacter sp. M21] Length = 872 Score = 274 bits (700), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 140/288 (48%), Positives = 185/288 (64%), Gaps = 1/288 (0%) Query: 7 LPKRLQPMLATLTDAPFDDPGWVFEDKYDGFRMIAKIRRGKVALYSRNGKIISRSYIEVA 66 +P RL PMLA PFDDP W+FE K DG+R IA+I G+ LYSRN ++ + + Sbjct: 219 MPHRLTPMLARSAAEPFDDPDWLFEIKLDGYRAIAEIEDGEALLYSRNNLSFNKRFPAIV 278 Query: 67 KALEGVKGDAVIDGELVAIGKDGVSHFQLLQNALRH-EAKLLYCAFDLMFADGEDLRALP 125 ++L + AV+DGE+VA+ + G S+FQLLQN R E + Y AFDL++ DGEDLRA P Sbjct: 279 QSLASLPVTAVLDGEVVALDEKGRSYFQLLQNNRRTGEGNIYYYAFDLLYVDGEDLRAEP 338 Query: 126 LLERKKRLKAILPRHKLVAFSNHRKGKGTKFFADAERRHLEGIMAKRADSPYASGRRTAD 185 L RK+RL+++LP V F +H + G +FF A +LEGI+AKRADS Y +GRR+ D Sbjct: 339 LEARKERLRSLLPELPWVRFGDHIREYGRQFFELARENNLEGILAKRADSRYLAGRRSGD 398 Query: 186 WLKVKTAQRQEVVIAGFTAPRRTRPFFGALVLAVREGDAWRYIGHVGTGFSHQVLEELHG 245 WLK+K +QE VI GFT PR +R G+LVL V E D YIG G GF L+E++ Sbjct: 399 WLKIKIRLQQEAVICGFTQPRGSRKGLGSLVLGVYENDELVYIGLTGGGFDDAGLKEMYA 458 Query: 246 RLMKLKTAKSPFPAKVKDEQVTTWVRPALVAEVKFAEWTSKGELRQPV 293 L L +SPF +VK TWV+P LV EV+FAEWT + +RQP+ Sbjct: 459 ALQLLVQPESPFRQQVKTSMPATWVKPVLVCEVEFAEWTDENVMRQPI 506 >ref|YP_002136955.1| DNA ligase D [Geobacter bemidjiensis Bem] gb|ACH37159.1| DNA ligase D, ATP-dependent [Geobacter bemidjiensis Bem] Length = 871 Score = 271 bits (692), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 140/288 (48%), Positives = 183/288 (63%), Gaps = 1/288 (0%) Query: 7 LPKRLQPMLATLTDAPFDDPGWVFEDKYDGFRMIAKIRRGKVALYSRNGKIISRSYIEVA 66 +P RL PMLA PFDDP W+FE K DG+R IA+I G+V LYSRN ++ + + Sbjct: 217 MPHRLTPMLANSAAEPFDDPDWLFEIKLDGYRAIAEIEGGEVQLYSRNNLSFNKRFPAIV 276 Query: 67 KALEGVKGDAVIDGELVAIGKDGVSHFQLLQNALRH-EAKLLYCAFDLMFADGEDLRALP 125 ++L + AV+DGE+VA+ G S FQLLQN+LR E + Y AFDL++ DGEDLRA P Sbjct: 277 QSLASLPVTAVLDGEVVALDAKGRSSFQLLQNSLRTGEKNIYYYAFDLLYLDGEDLRAEP 336 Query: 126 LLERKKRLKAILPRHKLVAFSNHRKGKGTKFFADAERRHLEGIMAKRADSPYASGRRTAD 185 L RK+RL+ +LP V FS+H + G +FF A +LEGI+AKRADS Y +GRR+ D Sbjct: 337 LQARKERLRNLLPELPWVRFSDHIREYGRQFFELARENNLEGILAKRADSRYFAGRRSGD 396 Query: 186 WLKVKTAQRQEVVIAGFTAPRRTRPFFGALVLAVREGDAWRYIGHVGTGFSHQVLEELHG 245 WLK+K +QE VI GFT PR +R G+LVL V E YIG G GF L+E++ Sbjct: 397 WLKIKIRLQQEAVICGFTQPRGSRKGLGSLVLGVYENGELVYIGLTGGGFDDVGLKEMYA 456 Query: 246 RLMKLKTAKSPFPAKVKDEQVTTWVRPALVAEVKFAEWTSKGELRQPV 293 L L +SPF +VK TW +P LV EV+FAEWT + +R P+ Sbjct: 457 ALQPLIQPESPFRQQVKTSMPVTWTKPVLVCEVEFAEWTDENVMRLPI 504