BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0008.p (224 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774662.1| DNA ligase [Bradyrhizobium japonicum USDA 110] ... 325 3e-87 ref|ZP_01047238.1| DNA ligase-like protein [Nitrobacter sp. Nb-3... 264 6e-69 ref|YP_001372844.1| DNA ligase D [Ochrobactrum anthropi ATCC 491... 170 1e-40 ref|YP_003692763.1| DNA ligase D [Starkeya novella DSM 506] >gi|... 168 5e-40 ref|YP_002822686.1| ATP-dependent DNA ligase [Sinorhizobium fred... 167 1e-39 >ref|NP_774662.1| DNA ligase [Bradyrhizobium japonicum USDA 110] dbj|BAC53287.1| blr8022 [Bradyrhizobium japonicum USDA 110] Length = 306 Score = 325 bits (834), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 152/191 (79%), Positives = 173/191 (90%) Query: 31 VAFQRQKPANIGVKAPLPGFIEPALATSIEKVPSGERWIHEIKFDGYRVQVHLANETAKI 90 +AF R+KPA +GVKAP PGF+EPALA+SIEKVPSG RWIHEIKFDGYRVQV LANE ++ Sbjct: 1 MAFHRRKPAAVGVKAPFPGFVEPALASSIEKVPSGARWIHEIKFDGYRVQVQLANEAVRV 60 Query: 91 FTRRGHDWTHRFKKVAHDAWRIKASSAVVDGEIVVPAADGTTDFSVLQNELKGKSKSIVL 150 FTRRGHDWT+RFKKVA DAW IKA SA+VDGEIVVPAADGTTDFSVLQNELKGKS SIVL Sbjct: 61 FTRRGHDWTNRFKKVADDAWHIKAGSAIVDGEIVVPAADGTTDFSVLQNELKGKSTSIVL 120 Query: 151 VAFDLLYLNGRDIRKLPLLQRKAELRKILSGTDIQFSESFEIDGREMFAHACKLGLEGVV 210 VAFDLLYLNGRD+RKLPL QRK EL+KI+ GT+IQFSESFE++G ++FAHACK+GLEG+V Sbjct: 121 VAFDLLYLNGRDLRKLPLFQRKTELKKIIDGTEIQFSESFELEGADIFAHACKIGLEGIV 180 Query: 211 SKVRGSGLSNG 221 SKVR S ++G Sbjct: 181 SKVRDSAYASG 191 >ref|ZP_01047238.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] gb|EAQ34820.1| DNA ligase-like protein [Nitrobacter sp. Nb-311A] Length = 247 Score = 264 bits (675), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 123/173 (71%), Positives = 147/173 (84%) Query: 41 IGVKAPLPGFIEPALATSIEKVPSGERWIHEIKFDGYRVQVHLANETAKIFTRRGHDWTH 100 IG +A PGFI PALA+ IE+VP G+RWIHEIKFDGYRVQVHL + K+FTRRG+DWT Sbjct: 9 IGSQANFPGFIPPALASPIERVPKGKRWIHEIKFDGYRVQVHLVEKNVKVFTRRGNDWTK 68 Query: 101 RFKKVAHDAWRIKASSAVVDGEIVVPAADGTTDFSVLQNELKGKSKSIVLVAFDLLYLNG 160 RF+K+A DA RI A +A++DGE+VVPA DGTTDFSVLQNEL+G+S++IVLVAFDLLYL+G Sbjct: 69 RFRKIADDASRIPAVTAIIDGEVVVPAEDGTTDFSVLQNELRGRSRNIVLVAFDLLYLDG 128 Query: 161 RDIRKLPLLQRKAELRKILSGTDIQFSESFEIDGREMFAHACKLGLEGVVSKV 213 D+R LPL QRK L+ ++ GT +QFSESF IDGREMF HACK+GLEGVVSKV Sbjct: 129 HDLRSLPLHQRKDALKALVGGTAVQFSESFTIDGREMFDHACKVGLEGVVSKV 181 >ref|YP_001372844.1| DNA ligase D [Ochrobactrum anthropi ATCC 49188] gb|ABS17015.1| DNA ligase D [Ochrobactrum anthropi ATCC 49188] Length = 834 Score = 170 bits (431), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 81/182 (44%), Positives = 123/182 (67%), Gaps = 4/182 (2%) Query: 44 KAPLPGFIEPALATSIEKVPSGERWIHEIKFDGYRVQVHLANETAKIFTRRGHDWTHRF- 102 K P+P F++P LAT + P G RW+HEIKFDGYR+Q H+A +++R G DWT +F Sbjct: 221 KRPMPSFVKPELATLVSVAPDGNRWLHEIKFDGYRIQAHIAGGKVALYSRGGLDWTEKFG 280 Query: 103 KKVAHDAWRIKASSAVVDGEIVVPAADGTTDFSVLQNELK-GKSKSIVLVAFDLLYLNGR 161 +++A D + A+ DGEIVV + G +DFS LQ +L+ G+S V FDLL+++G+ Sbjct: 281 QQIARDLVGTSVNEAIFDGEIVVESQSGLSDFSALQEDLREGRSDRFVYYLFDLLHVDGK 340 Query: 162 DIRKLPLLQRKAELRKILSGTD--IQFSESFEIDGREMFAHACKLGLEGVVSKVRGSGLS 219 D+R+ PL++RK L K++S + ++FSE+F+ DG + +AC LGLEG+VSK+R + Sbjct: 341 DLRQTPLVERKTLLEKLISASSSTLRFSEAFDADGAAVLKNACGLGLEGIVSKLREASYR 400 Query: 220 NG 221 +G Sbjct: 401 SG 402 >ref|YP_003692763.1| DNA ligase D [Starkeya novella DSM 506] gb|ADH88144.1| DNA ligase D [Starkeya novella DSM 506] Length = 842 Score = 168 bits (426), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 84/181 (46%), Positives = 124/181 (68%), Gaps = 4/181 (2%) Query: 45 APLPGFIEPALATSIEKVPSGERWIHEIKFDGYRVQVHLANETAKIFTRRGHDWTHRFKK 104 APLP FIEP LAT ++K PSG RW+HEIKFDGYR++V + + ++ TR G DWT RF Sbjct: 228 APLPDFIEPQLATLVDKAPSGTRWVHEIKFDGYRLEVRIEDGEVRLLTRTGLDWTARFGS 287 Query: 105 VAHDAWR-IKASSAVVDGEIVVPAADGTTDFSVLQNEL-KGKSKSIVLVAFDLLYLNGRD 162 +A++ + S+A++DGE+VV +G + FS LQ +L + ++ + AFDLL+L+G D Sbjct: 288 RLVEAFKALPLSNALIDGELVVEGGNGASHFSTLQADLSEERTDRFLFYAFDLLFLDGED 347 Query: 163 IRKLPLLQRKAELRKILSGT--DIQFSESFEIDGREMFAHACKLGLEGVVSKVRGSGLSN 220 +R PL++RKA L K+L+G +++SE F+ DG M HAC+L LEG+VSK+ + + Sbjct: 348 LRSKPLVERKAALAKLLAGVPDPLRYSEHFDEDGDVMLRHACRLSLEGLVSKLADAPYRS 407 Query: 221 G 221 G Sbjct: 408 G 408 >ref|YP_002822686.1| ATP-dependent DNA ligase [Sinorhizobium fredii NGR234] gb|AAQ87395.1| ATP-dependent DNA ligase [Sinorhizobium fredii NGR234] gb|ACP21933.1| putative ATP-dependent DNA ligase [Sinorhizobium fredii NGR234] Length = 850 Score = 167 bits (422), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 93/209 (44%), Positives = 130/209 (62%), Gaps = 12/209 (5%) Query: 28 SSSVAFQRQKPANI-------GVKA-PLPGFIEPALATSIEKVPSGERWIHEIKFDGYRV 79 ++S +RQ AN+ G KA PLP F+EPALAT + P+GERW+HEIKFDGYR+ Sbjct: 209 AASQQAERQAVANVPDPAKIKGAKAAPLPDFVEPALATLVSSAPAGERWLHEIKFDGYRL 268 Query: 80 QVHLANETAKIFTRRGHDWTHRFKKVAHDAWR-IKASSAVVDGEIVVPAADGTTDFSVLQ 138 Q + K+ TR G DWT +F K A + + +A++DGEIVV G +DFS LQ Sbjct: 269 QARIEAGRVKLLTRGGLDWTKKFGKTLVSASQALPIGTALIDGEIVVETGSGASDFSALQ 328 Query: 139 NEL-KGKSKSIVLVAFDLLYLNGRDIRKLPLLQRKAELRKILSGTD--IQFSESFEIDGR 195 +L +G+S FDLL+L+G D+R LPL +RK L +++ D I+FS FE +G+ Sbjct: 329 ADLSEGRSDRFRFYVFDLLHLDGYDLRPLPLTKRKELLGQLIGEADGIIRFSNHFEEEGK 388 Query: 196 EMFAHACKLGLEGVVSKVRGSGLSNGTHE 224 + HAC+L LEGVVSK+R + G ++ Sbjct: 389 LVLRHACRLSLEGVVSKLRDAPYRTGRNK 417