BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0189.0022.p (341 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770007.1| cellulase [Bradyrhizobium japonicum USDA 110] >... 512 e-143 ref|YP_004111047.1| glycoside hydrolase family protein [Rhodopse... 389 e-106 ref|ZP_08072865.1| glycoside hydrolase family 5 [Methylocystis s... 320 3e-85 gb|ABE60714.1| endo-1,4-beta-D-glucanase [uncultured bacterium] 285 8e-75 ref|YP_004146661.1| glycoside hydrolase family protein [Pseudoxa... 279 4e-73 >ref|NP_770007.1| cellulase [Bradyrhizobium japonicum USDA 110] dbj|BAC48632.1| blr3367 [Bradyrhizobium japonicum USDA 110] Length = 358 Score = 512 bits (1319), Expect = e-143, Method: Compositional matrix adjust. Identities = 244/341 (71%), Positives = 279/341 (81%) Query: 1 MTAFRILLQRAFVMLAAAGLAVPLIAPANAAGQGASPDAAALGRGINILGYDGIWEGYHN 60 M A R R+F + A A AN AGQ AS D LGRGINILGYDGIWEG N Sbjct: 18 MRAIRARWTRSFNLAATAVFLASFGFHANGAGQAASRDLPKLGRGINILGYDGIWEGGRN 77 Query: 61 APFRPENLAAIRRAGFSHVRINFFGFRHMDSGNILDDAVLRRLDAVIEQVLASRLVPILD 120 APFR +NL AIR+AGFSHVRINFFGF+ M GN+LD+ VLRRLDAVIE VLA L+PI+D Sbjct: 78 APFRLDNLTAIRKAGFSHVRINFFGFKFMGPGNVLDEIVLRRLDAVIEDVLARNLIPIVD 137 Query: 121 EHDTDLCQRDVAECGVKLKAFWRQIASRYAGKYPALVFEILNEPGGNMTSAEWNELLGDC 180 EHDT +CQRDV+ECG KL+AFWRQIA RYAGKYPALVFE+LNEPGG MTS +WN LL +C Sbjct: 138 EHDTHICQRDVSECGEKLRAFWRQIAERYAGKYPALVFEVLNEPGGQMTSGDWNSLLNEC 197 Query: 181 LAIIRGTNPKRNVVVAILNTEELPIDQLALPAGDRNLIVTFHYYLPLQFTHQGAPWSPTF 240 L IIR TNP R V+ A+LNT+++P+D LALPA DRNLIVTFHYY P++FTHQGAPWSPTF Sbjct: 198 LEIIRRTNPARPVIAAVLNTDDIPVDDLALPADDRNLIVTFHYYAPIRFTHQGAPWSPTF 257 Query: 241 SKIGPLRWGSAEDLAKASADFDKIRSWSEKEKRPIYLGEFGVYERAPAESRATYLSFMAR 300 S+IGPL WGS +D AKA+ADF+K+ W+EKEKRP+YLGEFGVYERAP++SRA YLSFMAR Sbjct: 258 SRIGPLDWGSPDDEAKAAADFEKMNLWAEKEKRPVYLGEFGVYERAPSDSRARYLSFMAR 317 Query: 301 RAERNGWAWAYWQFDHDFAAFDGARQRWKTDILRAIIPPVR 341 A+R GW+WAYWQFDHDFAAFD ARQ WK DILRA+IPP R Sbjct: 318 SADRLGWSWAYWQFDHDFAAFDSARQAWKPDILRALIPPQR 358 >ref|YP_004111047.1| glycoside hydrolase family protein [Rhodopseudomonas palustris DX-1] gb|ADU46314.1| glycoside hydrolase family 5 [Rhodopseudomonas palustris DX-1] Length = 344 Score = 389 bits (1000), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/330 (56%), Positives = 236/330 (71%), Gaps = 2/330 (0%) Query: 14 MLAAAGLAVPLIAPANAAGQGASPDAAA--LGRGINILGYDGIWEGYHNAPFRPENLAAI 71 ++A LA ++AP A + + A A + RG+NILGYDGIWEG +APFR ++L I Sbjct: 10 LIATMLLASAVVAPVAGATRVSDDFAMASRMARGVNILGYDGIWEGGVDAPFRLDHLDKI 69 Query: 72 RRAGFSHVRINFFGFRHMDSGNILDDAVLRRLDAVIEQVLASRLVPILDEHDTDLCQRDV 131 R+AGF HVRIN FGF+ MDS + L D L RLD VI +++ L+P++D HDT+ CQ + Sbjct: 70 RQAGFDHVRINLFGFKFMDSADRLSDVYLERLDLVIHSAISAGLIPVIDAHDTETCQIQI 129 Query: 132 AECGVKLKAFWRQIASRYAGKYPALVFEILNEPGGNMTSAEWNELLGDCLAIIRGTNPKR 191 C KL AFWRQ+A RYAG++P+LVFE+LNEPGG+MT A+WN LL + LA +R +NP+R Sbjct: 130 DVCRRKLAAFWRQVADRYAGQFPSLVFELLNEPGGHMTIAQWNSLLRELLATVRSSNPER 189 Query: 192 NVVVAILNTEELPIDQLALPAGDRNLIVTFHYYLPLQFTHQGAPWSPTFSKIGPLRWGSA 251 VVVA LNT E I+ L LPA DR +IVTFHYY P FTHQGAPWS + + WGS Sbjct: 190 IVVVAALNTPEASIESLELPATDRRIIVTFHYYEPFDFTHQGAPWSERLAGLPAREWGSE 249 Query: 252 EDLAKASADFDKIRSWSEKEKRPIYLGEFGVYERAPAESRATYLSFMARRAERNGWAWAY 311 +D AK +ADFD+++ ++ +E RP+YLGEFGVYERAP SR YL +AR AER GW WAY Sbjct: 250 QDRAKVAADFDRVQRFAAREARPVYLGEFGVYERAPRASRLRYLDDVARAAERRGWPWAY 309 Query: 312 WQFDHDFAAFDGARQRWKTDILRAIIPPVR 341 WQFDHDFA FD RQ W D+LRA+IPP R Sbjct: 310 WQFDHDFALFDTERQIWHADVLRALIPPRR 339 >ref|ZP_08072865.1| glycoside hydrolase family 5 [Methylocystis sp. ATCC 49242] gb|EFX99611.1| glycoside hydrolase family 5 [Methylocystis sp. ATCC 49242] Length = 339 Score = 320 bits (819), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 165/327 (50%), Positives = 217/327 (66%), Gaps = 4/327 (1%) Query: 12 FVMLAAAGLAVPLIAPANAAGQGASPDAAALGRGINILGYDGIWEGYHNAPFRPENLAAI 71 F+ + AA L++ A A A SP + A+ RG+N+LGYDG+W+G +APFR ++ I Sbjct: 8 FLKVLAALLSLHCAASAQPA---PSPASLAIRRGVNVLGYDGVWKGETDAPFRMDDFRLI 64 Query: 72 RRAGFSHVRINFFGFRHMDSGNILDDAVLRRLDAVIEQVLASRLVPILDEHDTDLCQRDV 131 R AGF HVRINFFG MD N LD VL RLD V++ A+ L +LD+HD LCQ+ Sbjct: 65 RAAGFDHVRINFFGLHFMDPKNRLDGEVLARLDRVLDAATAAGLSAVLDQHDNALCQQTP 124 Query: 132 AECGVKLKAFWRQIASRYAGKYPALVFEILNEPGGNMTSAEWNELLGDCLAIIRGTNPKR 191 C K+ AFWRQIA RYAG P +V+EILNEPGG+M++AEWN+ L LA IR +P R Sbjct: 125 PACKEKIVAFWRQIAKRYAGARPRVVYEILNEPGGHMSAAEWNDALAAALAEIRAADPDR 184 Query: 192 NVVVAILNTEEL-PIDQLALPAGDRNLIVTFHYYLPLQFTHQGAPWSPTFSKIGPLRWGS 250 V+VA LN ++ I++L +P DRNLIVT HYY P++FTHQGAPWS + + + WG+ Sbjct: 185 AVIVAALNAGDVHDIEKLDIPPQDRNLIVTVHYYEPMRFTHQGAPWSSELASLRDVAWGA 244 Query: 251 AEDLAKASADFDKIRSWSEKEKRPIYLGEFGVYERAPAESRATYLSFMARRAERNGWAWA 310 E DF + W+ +KRPIYLGEFGVY+ AP SRA +++ +A+ A+ +GW WA Sbjct: 245 EESRRMVDDDFAVVARWAAAQKRPIYLGEFGVYDSAPPLSRAAWMAHVAQTAQNHGWGWA 304 Query: 311 YWQFDHDFAAFDGARQRWKTDILRAII 337 YWQFDHDFA FD A WK L A++ Sbjct: 305 YWQFDHDFALFDTASHGWKQPFLDALM 331 >gb|ABE60714.1| endo-1,4-beta-D-glucanase [uncultured bacterium] Length = 506 Score = 285 bits (729), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 151/335 (45%), Positives = 204/335 (60%), Gaps = 11/335 (3%) Query: 8 LQRAFVMLAAAGLAVPLIAPANAAGQGASPDAAALGRGINILGYDGIWEGYHNAPFRPEN 67 L FVML A L +P+ A A+ RG+NI+GYD IW A F+P++ Sbjct: 7 LSGLFVMLCA--LVMPVAILAQPTSLTPEAQTQAMRRGVNIIGYDPIWSDPSKARFQPKH 64 Query: 68 LAAIRRAGFSHVRINFFGFRHMDSGNILDDAVLRRLDAVIEQVLASRLVPILDEHDTDLC 127 I+ GF VR+N F HMD N L A L+ LD V++ L + L ILD+H+ + C Sbjct: 65 FKIIKDGGFDAVRVNLHAFAHMDGNNRLKPAYLKTLDGVVKGALEAGLTVILDQHNFNEC 124 Query: 128 QRDVAECGVKLKAFWRQIASRYAGKYPALVFEILNEPGGNM-TSAEWNELLGDCLAIIRG 186 DV C VKLKAFW QI+ Y P +VFEILNEP N+ A WN+++ + L+IIR Sbjct: 125 ATDVPACRVKLKAFWTQISEIYKDAPPQVVFEILNEP--NLAVDAVWNDMVAENLSIIRV 182 Query: 187 TNPKRNVVVAIL---NTEELPIDQLALPAGDRNLIVTFHYYLPLQFTHQGAPWSPTFSKI 243 TNP RNV++ N ++LP L LP DR++IVTFHYY P++FTHQGA WSP ++++ Sbjct: 183 TNPTRNVIIGPQSWNNLDQLP--NLKLPENDRHIIVTFHYYTPMEFTHQGASWSPQYTQL 240 Query: 244 GPLRWGSAEDLAKASADFDKIRSWSEKEKRPIYLGEFGVYERAPAESRATYLSFMARRAE 303 G + WG+ D + A+FDK+ +WS+ KRPI LGEFG Y++AP SR Y +AR AE Sbjct: 241 G-ITWGTKGDRDQLEANFDKVAAWSKANKRPILLGEFGAYDKAPLASRIAYTEAVARAAE 299 Query: 304 RNGWAWAYWQFDHDFAAFDGARQRWKTDILRAIIP 338 G+AW+YWQFD +F +D +W I A+IP Sbjct: 300 ARGFAWSYWQFDSNFVVYDVPADKWNAPIHGALIP 334 >ref|YP_004146661.1| glycoside hydrolase family protein [Pseudoxanthomonas suwonensis 11-1] gb|ADV27430.1| glycoside hydrolase family 5 [Pseudoxanthomonas suwonensis 11-1] Length = 339 Score = 279 bits (714), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 151/332 (45%), Positives = 197/332 (59%), Gaps = 12/332 (3%) Query: 18 AGLAVPLIAP-ANAAGQGASPDAAA-----LGRGINILGYDGIWEGYHNAPFRPENLAAI 71 AGL + L+A NA P +AA +G G+N+LGYD W+ +R E+ A I Sbjct: 8 AGLCLGLMALLPNAMAADPRPLSAADQVARMGAGVNVLGYDPYWKAGEQGNYREEHFAKI 67 Query: 72 RRAGFSHVRINFFGFRHMDSGNILDDAVLRRLDAVIEQVLASRLVPILDEHDTDLCQRDV 131 R AGF VR+ F F H+D N LD L +LD VI + L ILD HD + C +DV Sbjct: 68 REAGFGTVRVALFTFPHLDEANRLDPQWLAKLDQVIGWGTGNGLQVILDVHDFNDCVKDV 127 Query: 132 AECGVKLKAFWRQIASRYAGKYPALVFEILNEPGGNMTSAEWNELLGDCLAIIRGTNPKR 191 C VKL+ W Q+ RYAG+ +VFE+LNEP G + + WN L L ++R TNP R Sbjct: 128 PACEVKLEQVWTQLGERYAGQPDQVVFELLNEPHGALDAKAWNGLFAKLLEVVRRTNPTR 187 Query: 192 NVVVAIL---NTEELPIDQLALPAGDRNLIVTFHYYLPLQFTHQGAPW-SPTFSKIGPLR 247 NVVV N EEL D L LP DR+LIVTFHYY P FTHQGA W P FS+ +R Sbjct: 188 NVVVGPTRWNNLEEL--DTLQLPEDDRHLIVTFHYYEPFAFTHQGASWVEPQFSEKTGVR 245 Query: 248 WGSAEDLAKASADFDKIRSWSEKEKRPIYLGEFGVYERAPAESRATYLSFMARRAERNGW 307 + + +AK +DFDK+++WS K RPI LGE+G Y++AP E R + +AR AE+ G+ Sbjct: 246 FNTPGQVAKIESDFDKVKAWSRKHDRPILLGEYGAYDKAPMEDRVYWTRTVARAAEKRGF 305 Query: 308 AWAYWQFDHDFAAFDGARQRWKTDILRAIIPP 339 A YWQF DF +D R++W +L A+IPP Sbjct: 306 AHLYWQFSSDFVLYDFEREKWVQPLLEAVIPP 337