BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0003.p (384 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003693838.1| FMN-dependent alpha-hydroxy acid dehydrogena... 489 e-136 ref|YP_003908791.1| FMN-dependent alpha-hydroxy acid dehydrogena... 468 e-129 ref|YP_001756071.1| L-lactate dehydrogenase (cytochrome) [Methyl... 465 e-129 ref|YP_003741615.1| L-lactate dehydrogenase [Erwinia billingiae ... 464 e-128 ref|YP_003979323.1| FMN-dependent dehydrogenase family protein 2... 459 e-127 >ref|YP_003693838.1| FMN-dependent alpha-hydroxy acid dehydrogenase [Starkeya novella DSM 506] gb|ADH89219.1| FMN-dependent alpha-hydroxy acid dehydrogenase [Starkeya novella DSM 506] Length = 421 Score = 489 bits (1259), Expect = e-136, Method: Compositional matrix adjust. Identities = 238/382 (62%), Positives = 289/382 (75%) Query: 1 VPRHLRRYLTLDDFEPQARRLLPKFLYGYISGGAETDMARTDNRRAFEEYGFVPRVLQDV 60 VPR R YL LDDFE ARR LP+ +YGY+SG ET + R+A+ + VPR L DV Sbjct: 34 VPRRFRNYLALDDFERAARRHLPRMIYGYVSGAVETGSGMREARQAYADLALVPRTLMDV 93 Query: 61 SGRDQTTTLFGKSYASPFGIPPMGSSALCAYRGDIVLTRAASAANIPMILSASSLITLED 120 + R Q LFG SYASPFGI P+G +A+ AYR DI L AA AA +PMILSASSLI LED Sbjct: 94 AARHQARGLFGPSYASPFGIAPLGGAAMVAYRADIELAAAARAAQVPMILSASSLIKLED 153 Query: 121 VRAANPSAWYQAYLAGLPERIEPLVDRVAAAGYDTFVVTADVPVPPNRENNIRNGFQVPL 180 V NP AW+QAYLAG RI+ +VDRVA+AG+ T VVTAD PVP NRENN R+GF +PL Sbjct: 154 VHRENPDAWFQAYLAGHQPRIDAMVDRVASAGFGTLVVTADTPVPGNRENNTRSGFSMPL 213 Query: 181 EITPRVFWDTITHPDWLLNTWARTVFNHGMPHFENMDAKQGPPVLSKNLMRNIGSRDQLA 240 ITP+V D++ HP WL+ T RT HG+PHFENMDA QGPP+ S++ +RN+ SRDQLA Sbjct: 214 RITPKVALDSVLHPRWLIGTVGRTFLRHGVPHFENMDAAQGPPMFSRDFVRNMNSRDQLA 273 Query: 241 WKHVELIRKRWRGKLVVKGLIAPEDARLAREHGCDGVMVSNHGGRQLDYTVSGLRTLPEI 300 W HVE IR+RW+GKLVVKGL+A D R AR+ G DGV++SNHGGRQLDY ++ +R LPE+ Sbjct: 274 WCHVEAIRRRWKGKLVVKGLLAAADVRKARQIGVDGVIISNHGGRQLDYALAPIRALPEL 333 Query: 301 AAQKGNMTVMVDGGIRRGTDVIKALALGADFVWVGRPFLYAAVVAGEAGVARAISLLHAE 360 A+ MTVM+DGGIRRGTDV+KALALGADFV++GRPFLYAA + G GV AI LL E Sbjct: 334 RAEAQEMTVMLDGGIRRGTDVLKALALGADFVFLGRPFLYAASLGGTEGVLHAIRLLSEE 393 Query: 361 IDRDLALLGIRSLKEITPDLVR 382 I RD+AL+G+R+L E+TPD+VR Sbjct: 394 IHRDMALMGLRTLDELTPDMVR 415 >ref|YP_003908791.1| FMN-dependent alpha-hydroxy acid dehydrogenase [Burkholderia sp. CCGE1003] gb|ADN59500.1| FMN-dependent alpha-hydroxy acid dehydrogenase [Burkholderia sp. CCGE1003] Length = 410 Score = 468 bits (1203), Expect = e-129, Method: Compositional matrix adjust. Identities = 231/382 (60%), Positives = 284/382 (74%), Gaps = 1/382 (0%) Query: 1 VPRHLRRYLTLDDFEPQARRLLPKFLYGYISGGAETDMARTDNRRAFEEYGFVPRVLQDV 60 VP LRRYL+LDDFE ARR LP+ ++GYISG AE + + DN+RA+ EY FV RVL+DV Sbjct: 14 VPNALRRYLSLDDFEIAARRHLPRPIFGYISGAAERNASLDDNQRAYSEYRFVTRVLRDV 73 Query: 61 SGRDQTTTLFGKSYASPFGIPPMGSSALCAYRGDIVLTRAASAANIPMILSASSLITLED 120 S R Q TTL GK+Y +PFGI PMG SAL AYRGD+V +AA A IPMI+S SSLI LE Sbjct: 74 SRRTQATTLLGKTYRAPFGIAPMGISALSAYRGDLVQAQAAREAAIPMIMSGSSLIPLET 133 Query: 121 VRAANPSAWYQAYLAGLPERIEPLVDRVAAAGYDTFVVTADVPVPPNRENNIRNGFQVPL 180 V P W+QAYL G PE+I L++RV AGYDT VVT D V NRENNIR GF PL Sbjct: 134 VAKEAPDTWFQAYLPGEPEKIRALIERVERAGYDTLVVTVDTAVLANRENNIRTGFSTPL 193 Query: 181 EITPRVFWDTITHPDWLLNTWARTVFNHGMPHFENMDAKQGPPVLSKNLMRNIGSRDQLA 240 + + R+ WD +T P WL+ T RT+ HGMPHFEN A +G P+ S ++R+ G++D L Sbjct: 194 KPSIRLAWDGVTRPRWLVGTALRTLVKHGMPHFENSYATRGAPIFSGRVVRDFGAKDHLN 253 Query: 241 WKHVELIRKRWRGKLVVKGLIAPEDARLAREHGCDGVMVSNHGGRQLDYTVSGLRTLPEI 300 W HV IR +W+GKLVVKG++A EDA AR+HG DG++VSNHGGRQLD T + LR LP I Sbjct: 254 WTHVRQIRSQWKGKLVVKGIMAAEDALAARDHGVDGIIVSNHGGRQLDGTAAPLRVLPRI 313 Query: 301 AAQKG-NMTVMVDGGIRRGTDVIKALALGADFVWVGRPFLYAAVVAGEAGVARAISLLHA 359 A G +M VM+DGGIRRGTDV+KALALGADFV+VGRPF YAA VAG+AGVA AI +LHA Sbjct: 314 ADAVGRDMAVMIDGGIRRGTDVLKALALGADFVFVGRPFNYAASVAGKAGVAHAIGILHA 373 Query: 360 EIDRDLALLGIRSLKEITPDLV 381 E+ R+L LLG+ S+ E++PD++ Sbjct: 374 EVQRNLGLLGLNSIDELSPDVL 395 >ref|YP_001756071.1| L-lactate dehydrogenase (cytochrome) [Methylobacterium radiotolerans JCM 2831] gb|ACB25388.1| L-lactate dehydrogenase (cytochrome) [Methylobacterium radiotolerans JCM 2831] Length = 435 Score = 465 bits (1196), Expect = e-129, Method: Compositional matrix adjust. Identities = 217/383 (56%), Positives = 284/383 (74%) Query: 1 VPRHLRRYLTLDDFEPQARRLLPKFLYGYISGGAETDMARTDNRRAFEEYGFVPRVLQDV 60 P R L LDDFE AR+LLP ++ Y+SGG ET A +R A+ +Y VPR+++D Sbjct: 48 TPYRFRDLLALDDFERHARKLLPPMIFQYVSGGVETGSALAQSRGAYADYALVPRLMRDT 107 Query: 61 SGRDQTTTLFGKSYASPFGIPPMGSSALCAYRGDIVLTRAASAANIPMILSASSLITLED 120 S RD TTLFG++YA+PFG+ P+G +A AYRGD+VL AA N+PM LSASSLI LED Sbjct: 108 SARDTATTLFGQTYAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSASSLIRLED 167 Query: 121 VRAANPSAWYQAYLAGLPERIEPLVDRVAAAGYDTFVVTADVPVPPNRENNIRNGFQVPL 180 V A NP AW+Q YL G RI+ L+DRV A GY TFVVTAD P NRE+NIR+GF +P+ Sbjct: 168 VHAQNPQAWFQGYLPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPI 227 Query: 181 EITPRVFWDTITHPDWLLNTWARTVFNHGMPHFENMDAKQGPPVLSKNLMRNIGSRDQLA 240 ++TP+V W ++THP WLL T ART HG PHFEN +A++GPP++S+ +RN +RDQL+ Sbjct: 228 KVTPKVAWQSVTHPRWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLS 287 Query: 241 WKHVELIRKRWRGKLVVKGLIAPEDARLAREHGCDGVMVSNHGGRQLDYTVSGLRTLPEI 300 WK++E IRKRW G L+VKGL+APED +AR G DGV++S HGGRQLDY V+ L LPEI Sbjct: 288 WKNLEAIRKRWSGNLLVKGLLAPEDVDIARACGADGVILSTHGGRQLDYAVAPLDVLPEI 347 Query: 301 AAQKGNMTVMVDGGIRRGTDVIKALALGADFVWVGRPFLYAAVVAGEAGVARAISLLHAE 360 AA+KG + ++VD G+RRGTDV+KALALGADFV +GRPF++AA + G GV A+ +L E Sbjct: 348 AARKGGLKIIVDSGVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHAMRILKEE 407 Query: 361 IDRDLALLGIRSLKEITPDLVRK 383 ++RD+AL+G+ L E+ PD +R+ Sbjct: 408 LNRDMALIGVNRLSELNPDFLRR 430 >ref|YP_003741615.1| L-lactate dehydrogenase [Erwinia billingiae Eb661] emb|CAX59768.1| L-lactate dehydrogenase (Cytochrome) [Erwinia billingiae Eb661] Length = 413 Score = 464 bits (1193), Expect = e-128, Method: Compositional matrix adjust. Identities = 221/384 (57%), Positives = 286/384 (74%), Gaps = 1/384 (0%) Query: 1 VPRHLRRYLTLDDFEPQARRLLPKFLYGYISGGAETDMARTDNRRAFEEYGFVPRVLQDV 60 +PRH R L LDDFE ARR LP +Y Y++GG ET N A++ Y F+PR+ +DV Sbjct: 30 LPRHFRDLLALDDFERHARRRLPNMIYQYVAGGVETGRGIAGNFEAYQRYAFLPRMFRDV 89 Query: 61 SGRDQTTTLFGKSYASPFGIPPMGSSALCAYRGDIVLTRAASAANIPMILSASSLITLED 120 SGRDQ TTLFG +Y PFG+ P+G ++ AY+ D+ L +AA N+PMILSASSL+ LED Sbjct: 90 SGRDQRTTLFGHTYQHPFGVAPLGGASFVAYQADVALAKAAREMNVPMILSASSLVKLED 149 Query: 121 VRAANPSAWYQAYLAGLPERIEPLVDRVAAAGYDTFVVTADVPVPPNRENNIRNGFQVPL 180 V AANP AW+QAYLAG RI+ LVDRVAAAGY T VVT D P+ NRE+N R+GF +P+ Sbjct: 150 VHAANPDAWFQAYLAGDQPRIDRLVDRVAAAGYKTLVVTGDTPMLGNREHNTRSGFSMPI 209 Query: 181 EITPRVFWDTITHPDWLLNTWARTVFNHGMPHFENMDAKQGPPVLSKNLMRNIGSRDQLA 240 +ITP+V +++ P WLL T A+T HG PHFEN DA++GPP++S + RN +RD+L Sbjct: 210 KITPKVAFESAMSPRWLLGTVAQTFLRHGAPHFENTDAERGPPMMSSKV-RNTQARDKLN 268 Query: 241 WKHVELIRKRWRGKLVVKGLIAPEDARLAREHGCDGVMVSNHGGRQLDYTVSGLRTLPEI 300 WK+VE IRK+WRG LVVKGL++PEDA +AR+ G D V++SNHGGRQLDYT L +L EI Sbjct: 269 WKNVEAIRKKWRGNLVVKGLMSPEDAFIARDLGADAVILSNHGGRQLDYTFPPLYSLEEI 328 Query: 301 AAQKGNMTVMVDGGIRRGTDVIKALALGADFVWVGRPFLYAAVVAGEAGVARAISLLHAE 360 AA+KG M V++D GIRRGTDV+KA+ALGADFV++GRPFLY AV+ G+A V A+ +L E Sbjct: 329 AAKKGAMKVIIDSGIRRGTDVMKAMALGADFVFLGRPFLYGAVIGGQACVEHAMHILRDE 388 Query: 361 IDRDLALLGIRSLKEITPDLVRKF 384 IDRDLAL+G+R+ E+ +R+ Sbjct: 389 IDRDLALIGVRTPGELDSSYLRRM 412 >ref|YP_003979323.1| FMN-dependent dehydrogenase family protein 2 [Achromobacter xylosoxidans A8] gb|ADP16608.1| FMN-dependent dehydrogenase family protein 2 [Achromobacter xylosoxidans A8] Length = 405 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 219/383 (57%), Positives = 283/383 (73%) Query: 1 VPRHLRRYLTLDDFEPQARRLLPKFLYGYISGGAETDMARTDNRRAFEEYGFVPRVLQDV 60 +PR L R L+LDDFE A+R LP+ ++GY++G AE + + NR AF +Y F PRVL DV Sbjct: 20 LPRALARMLSLDDFEAAAKRRLPRPIFGYVAGAAEDNQSLRGNRSAFAQYSFSPRVLVDV 79 Query: 61 SGRDQTTTLFGKSYASPFGIPPMGSSALCAYRGDIVLTRAASAANIPMILSASSLITLED 120 S R Q T +FG+ YASPFGI PMG SAL AYRGDIVL RAA IP ILS +SLI +E+ Sbjct: 80 SRRTQQTEIFGRRYASPFGIAPMGISALSAYRGDIVLARAAREQGIPAILSGTSLIPMEE 139 Query: 121 VRAANPSAWYQAYLAGLPERIEPLVDRVAAAGYDTFVVTADVPVPPNRENNIRNGFQVPL 180 V A P W+QAYL G P+RI+ LV+R AGY+T V+T D+PV NRENN+R GF PL Sbjct: 140 VIRAAPGTWFQAYLPGDPQRIDALVERARRAGYETLVLTVDIPVSANRENNVRTGFSTPL 199 Query: 181 EITPRVFWDTITHPDWLLNTWARTVFNHGMPHFENMDAKQGPPVLSKNLMRNIGSRDQLA 240 + + R+ WD +T P WL T+ RT+ HGMPHFEN A +G P++S +++R+ +RD L+ Sbjct: 200 KPSLRLAWDGLTRPRWLAGTFMRTLLAHGMPHFENSFATRGAPIVSASVLRDFSARDHLS 259 Query: 241 WKHVELIRKRWRGKLVVKGLIAPEDARLAREHGCDGVMVSNHGGRQLDYTVSGLRTLPEI 300 W+HV IR +W G L++KG++ P+DA LAR+HG DG++VSNHGGRQLD VS LR LP + Sbjct: 260 WEHVARIRSQWPGTLIIKGILHPQDAALARQHGADGIIVSNHGGRQLDGAVSPLRALPGV 319 Query: 301 AAQKGNMTVMVDGGIRRGTDVIKALALGADFVWVGRPFLYAAVVAGEAGVARAISLLHAE 360 A G MTVM+D G+RRG+DV+KALALGA FV+VGRPF YAA V GEAGV+ AI LL AE Sbjct: 320 VAAAGGMTVMMDSGVRRGSDVLKALALGASFVFVGRPFNYAAAVGGEAGVSHAIGLLRAE 379 Query: 361 IDRDLALLGIRSLKEITPDLVRK 383 IDR++A+LGI +L+E+ DL+ + Sbjct: 380 IDRNMAMLGINNLREMQADLLMR 402