BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0070.p (420 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004154706.1| major facilitator superfamily protein [Vario... 520 e-145 ref|YP_002945135.1| major facilitator superfamily protein [Vario... 510 e-142 ref|ZP_02891441.1| major facilitator superfamily MFS_1 [Burkhold... 474 e-131 ref|YP_772317.1| major facilitator transporter [Burkholderia amb... 473 e-131 ref|ZP_03586296.1| major facilitator superfamily MFS_1 [Burkhold... 468 e-130 >ref|YP_004154706.1| major facilitator superfamily protein [Variovorax paradoxus EPS] gb|ADU36595.1| major facilitator superfamily MFS_1 [Variovorax paradoxus EPS] Length = 438 Score = 520 bits (1340), Expect = e-145, Method: Compositional matrix adjust. Identities = 273/408 (66%), Positives = 328/408 (80%) Query: 11 QRAKPFYGWYVVTAAFVVTLIGFGSAYTFSSFVESLQREFGASRGSVSLVFSFAGFLYFG 70 QRA+ F GW VV AF VTL+GFGSAYTFS+F+ESLQR+FGASRGSVSLVFS AGFLYFG Sbjct: 13 QRARIFRGWLVVAGAFAVTLVGFGSAYTFSAFLESLQRDFGASRGSVSLVFSLAGFLYFG 72 Query: 71 LGMISGPLADRWGARRLAGLGMVLVGLGMVLAGQAQTILQVYAAYSIGIGAGVGCAYVPT 130 LG+ISGPLADRWG+RRLA GMVLVGLG+ LAGQAQ++ Q+Y AY +G+G GVG +YVP Sbjct: 73 LGVISGPLADRWGSRRLAVAGMVLVGLGLALAGQAQSLTQIYLAYGLGVGLGVGASYVPV 132 Query: 131 LGAVQRWFVRRRGFASGLAVSGIGVGTLVMPPLATWLITSLGWRDAYVVLGVVAAVVGIG 190 LGAVQRWFV+RRGFASGLAVSGIGVGTLVMPPLA++LI +GWR AY+ LG +A VVG+G Sbjct: 133 LGAVQRWFVKRRGFASGLAVSGIGVGTLVMPPLASFLIEGIGWRHAYLALGALAVVVGVG 192 Query: 191 ASLLIADDPRRYGTGPDGDPLDTVSGAPLRQGVSIRAAVRTARFAGLYAACLVSAFGVFV 250 +L I +DPR G GPDGDP + + G ++R AV + RFAGL+AACL+S+FG+FV Sbjct: 193 MALFIENDPRERGLGPDGDPPQPQASSAKPAGATVREAVTSRRFAGLFAACLISSFGLFV 252 Query: 251 PFVHLVPFAVDHQVAPTLAVLLLGMIGVGSTAGRFFLGGIADRVGRDAFLVAMYLGMAVS 310 PFVHLVP+A+DH V T AVLLLG IGVGSTAGRFFLGG+ADR+GR L M GMA+S Sbjct: 253 PFVHLVPYALDHGVPSTSAVLLLGAIGVGSTAGRFFLGGLADRIGRQLALPLMSAGMALS 312 Query: 311 LAIWAIAGTFWSLTVFALLFGLFYGGWVAILPAVVTDHFGGRNVGGIIGILYTSVAVGTL 370 L +WA++G FW L VFA +G+FYGG+VA+LPA+V D+FGGRN+ IIG+LYTSVAVGTL Sbjct: 313 LVVWAVSGGFWGLAVFAFAYGVFYGGFVALLPALVMDYFGGRNISAIIGVLYTSVAVGTL 372 Query: 371 LGPSAAGFAYDIGHSYFIPVAISAAANLVAALIAIVTMRTASPIASVA 418 +GPSAAGFA+DI SY +P+ S ANLVAA I + R + + A Sbjct: 373 VGPSAAGFAFDISRSYLLPIVASIGANLVAAGILLAMARRDASVKPAA 420 >ref|YP_002945135.1| major facilitator superfamily protein [Variovorax paradoxus S110] gb|ACS19869.1| major facilitator superfamily MFS_1 [Variovorax paradoxus S110] Length = 430 Score = 510 bits (1314), Expect = e-142, Method: Compositional matrix adjust. Identities = 263/403 (65%), Positives = 322/403 (79%), Gaps = 2/403 (0%) Query: 12 RAKPFYGWYVVTAAFVVTLIGFGSAYTFSSFVESLQREFGASRGSVSLVFSFAGFLYFGL 71 R + F GW+VV AF VTL+GFGSAYTFS+F+ESLQR+FGASRGS SLVFS AGFLYFGL Sbjct: 10 RTRLFRGWFVVAGAFAVTLVGFGSAYTFSAFLESLQRDFGASRGSASLVFSLAGFLYFGL 69 Query: 72 GMISGPLADRWGARRLAGLGMVLVGLGMVLAGQAQTILQVYAAYSIGIGAGVGCAYVPTL 131 G++SGPLADRWGARRL+ GMVLVGLG+ AGQA+++ + AY +G+G G+G +YVP L Sbjct: 70 GVVSGPLADRWGARRLSVAGMVLVGLGLAAAGQARSLAEACLAYGLGVGLGIGASYVPVL 129 Query: 132 GAVQRWFVRRRGFASGLAVSGIGVGTLVMPPLATWLITSLGWRDAYVVLGVVAAVVGIGA 191 GAVQRWFVRRRGFASGLAVSGIGVGTL MPPLA+ LI +LGWR AY+ LGV+A VVG G Sbjct: 130 GAVQRWFVRRRGFASGLAVSGIGVGTLAMPPLASLLIEALGWRHAYLALGVLAVVVGAGM 189 Query: 192 SLLIADDPRRYGTGPDGDPLDTVSGAPLRQGVSIRAAVRTARFAGLYAACLVSAFGVFVP 251 +LLI DDPR G GPDGDP + + + G ++R A+ + RFAGLYAACL+S+FG+FVP Sbjct: 190 ALLIEDDPRHRGLGPDGDPPQPQAPSAVPAGSTLRDAISSRRFAGLYAACLISSFGLFVP 249 Query: 252 FVHLVPFAVDHQVAPTLAVLLLGMIGVGSTAGRFFLGGIADRVGRDAFLVAMYLGMAVSL 311 FVHLVP+A+DH + AVLLLG IG+GSTAGRFFLGG+ADR+GR L M GMA+SL Sbjct: 250 FVHLVPYALDHGLPAGSAVLLLGAIGIGSTAGRFFLGGLADRLGRQLALPLMSAGMALSL 309 Query: 312 AIWAIAGTFWSLTVFALLFGLFYGGWVAILPAVVTDHFGGRNVGGIIGILYTSVAVGTLL 371 +WA++ FW L FA +G+FYGG+VA+LPA+V D+FGGR++G I+G+LYTSVA GTL+ Sbjct: 310 VVWAVSSGFWGLAAFAFAYGVFYGGFVALLPALVMDYFGGRSIGAILGVLYTSVAFGTLV 369 Query: 372 GPSAAGFAYDIGHSYFIPVAISAAANLVAA--LIAIVTMRTAS 412 GPSAAGFA+DI SY +P+ S ANLVAA L+A+ R AS Sbjct: 370 GPSAAGFAFDISRSYLLPILASIGANLVAAGVLLAMSKARAAS 412 >ref|ZP_02891441.1| major facilitator superfamily MFS_1 [Burkholderia ambifaria IOP40-10] gb|EDT02967.1| major facilitator superfamily MFS_1 [Burkholderia ambifaria IOP40-10] Length = 420 Score = 474 bits (1220), Expect = e-131, Method: Compositional matrix adjust. Identities = 260/389 (66%), Positives = 307/389 (78%), Gaps = 7/389 (1%) Query: 8 AGYQRAKPFYGWYVVTAAFVVTLIGFGSAYTFSSFVESLQREFGASRGSVSLVFSFAGFL 67 +G + ++ FYGWYVV AAF VT +GFGSAYTFS+FVESLQR+F ASRG +SLVFS AGFL Sbjct: 2 SGPRASRVFYGWYVVAAAFAVTFVGFGSAYTFSAFVESLQRDFAASRGQISLVFSLAGFL 61 Query: 68 YFGLGMISGPLADRWGARRLAGLGMVLVGLGMVLAGQAQTILQVYAAYSIGIGAGVGCAY 127 YFG G++SGPLADR+G+RRLA GM+L G+ AG A T+LQVY AY +G+G GVGCAY Sbjct: 62 YFGFGIVSGPLADRFGSRRLAVAGMLLTAAGLAAAGAAHTLLQVYVAYGLGVGFGVGCAY 121 Query: 128 VPTLGAVQRWFVRRRGFASGLAVSGIGVGTLVMPPLATWLITSLGWRDAYVVLGVVAAVV 187 VP +GAVQRWFVRRRGFASGLAV+GIGVGTLVMPPLA+ LI +GWR AYV L +A VV Sbjct: 122 VPAVGAVQRWFVRRRGFASGLAVAGIGVGTLVMPPLASALIAHVGWRGAYVTLAAIAVVV 181 Query: 188 GIGASLLIADDPRRYGTGPDGDPLDTVSG-------APLRQGVSIRAAVRTARFAGLYAA 240 G G SLLI +DPR G PDGD T +G + + G ++R AV + FA LYAA Sbjct: 182 GAGMSLLIENDPRGRGLLPDGDVARTQAGDAMPGATSSVPAGATVREAVASRPFASLYAA 241 Query: 241 CLVSAFGVFVPFVHLVPFAVDHQVAPTLAVLLLGMIGVGSTAGRFFLGGIADRVGRDAFL 300 CLV +FGVFVPFVHLVP+A+DH VAP+ AVLLLG IGVGSTAGRFFLGG+ADR GR A L Sbjct: 242 CLVCSFGVFVPFVHLVPYALDHGVAPSTAVLLLGAIGVGSTAGRFFLGGLADRFGRRASL 301 Query: 301 VAMYLGMAVSLAIWAIAGTFWSLTVFALLFGLFYGGWVAILPAVVTDHFGGRNVGGIIGI 360 +AM+ GMAV+L WA AG+ +L FAL+FG+FYGGWVA+LPAVV D+FGGRNV IIG+ Sbjct: 302 LAMFAGMAVALVAWAGAGSVATLATFALVFGVFYGGWVAVLPAVVMDYFGGRNVSAIIGV 361 Query: 361 LYTSVAVGTLLGPSAAGFAYDIGHSYFIP 389 LYTSVA GTL+GP+AAGF YD G Y +P Sbjct: 362 LYTSVAFGTLIGPAAAGFIYDAGGGYLVP 390 >ref|YP_772317.1| major facilitator transporter [Burkholderia ambifaria AMMD] gb|ABI85983.1| major facilitator superfamily MFS_1 [Burkholderia ambifaria AMMD] Length = 420 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 260/389 (66%), Positives = 305/389 (78%), Gaps = 7/389 (1%) Query: 8 AGYQRAKPFYGWYVVTAAFVVTLIGFGSAYTFSSFVESLQREFGASRGSVSLVFSFAGFL 67 +G + ++ FYGWYVV AAF VT +GFGSAYTFS+FVESLQR+F ASRG +SLVFS AGFL Sbjct: 2 SGPRASRVFYGWYVVAAAFAVTFVGFGSAYTFSAFVESLQRDFAASRGQISLVFSLAGFL 61 Query: 68 YFGLGMISGPLADRWGARRLAGLGMVLVGLGMVLAGQAQTILQVYAAYSIGIGAGVGCAY 127 YFG G++SGPLADR+G+RRLA GM+L G+ AG A T+LQVY AY +G+G GVGCAY Sbjct: 62 YFGFGIVSGPLADRFGSRRLAVAGMLLTAAGLAAAGAAHTLLQVYVAYGLGVGFGVGCAY 121 Query: 128 VPTLGAVQRWFVRRRGFASGLAVSGIGVGTLVMPPLATWLITSLGWRDAYVVLGVVAAVV 187 VP +GAVQRWFVRRRGFASGLAV+GIGVGTLVMPPLA+ LI +GWR AYV L +A VV Sbjct: 122 VPAVGAVQRWFVRRRGFASGLAVAGIGVGTLVMPPLASALIAHVGWRGAYVTLAAIAVVV 181 Query: 188 GIGASLLIADDPRRYGTGPDGDPLDTVSGAPL-------RQGVSIRAAVRTARFAGLYAA 240 G G SLLI +DPR G PDGD +G L G ++R AV + FA LYAA Sbjct: 182 GAGMSLLIENDPRGRGLLPDGDVARKQAGDALPGAASAVPAGATVREAVASRPFASLYAA 241 Query: 241 CLVSAFGVFVPFVHLVPFAVDHQVAPTLAVLLLGMIGVGSTAGRFFLGGIADRVGRDAFL 300 CLV +FGVFVPFVHLVP+A+DH VAP+ AVLLLG IGVGSTAGRFFLGG+ADR GR A L Sbjct: 242 CLVCSFGVFVPFVHLVPYALDHGVAPSTAVLLLGAIGVGSTAGRFFLGGLADRFGRRASL 301 Query: 301 VAMYLGMAVSLAIWAIAGTFWSLTVFALLFGLFYGGWVAILPAVVTDHFGGRNVGGIIGI 360 +AM+ GMAV+L WA AG+ +L FAL+FG+FYGGWVA+LPAVV D+FGGRNV IIG+ Sbjct: 302 LAMFAGMAVALVAWAGAGSVATLAAFALVFGVFYGGWVAVLPAVVMDYFGGRNVSAIIGV 361 Query: 361 LYTSVAVGTLLGPSAAGFAYDIGHSYFIP 389 LYTSVA GTL+GP+AAGF YD G Y +P Sbjct: 362 LYTSVAFGTLIGPAAAGFIYDAGGGYLVP 390 >ref|ZP_03586296.1| major facilitator superfamily MFS_1 [Burkholderia multivorans CGD1] gb|EED99244.1| major facilitator superfamily MFS_1 [Burkholderia multivorans CGD1] Length = 441 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 262/406 (64%), Positives = 305/406 (75%), Gaps = 27/406 (6%) Query: 11 QRAKPFYGWYVVTAAFVVTLIGFGSAYTFSSFVESLQREFGASRGSVSLVFSFAGFLYFG 70 + ++ FYGWYVV AAF VT +GFGSAYTFS+FVE LQR+F ASRG +SLVFS AGFLYFG Sbjct: 6 RTSRVFYGWYVVAAAFAVTFVGFGSAYTFSAFVEPLQRDFAASRGQISLVFSLAGFLYFG 65 Query: 71 LGMISGPLADRWGARRLAGLGMVLVGLGMVLAGQAQTILQVYAAYSIGIGAGVGCAYVPT 130 G++SGPLADR+G+RRLA +GM+L G+ AG A+T+LQVYAAY +G+G GVGCAYVP Sbjct: 66 FGIVSGPLADRFGSRRLAVVGMLLTAAGLAAAGAARTLLQVYAAYGLGVGFGVGCAYVPA 125 Query: 131 LGAVQRWFVRRRGFASGLAVSGIGVGTLVMPPLATWLITSLGWRDAYVVLGVVAAVVGIG 190 +GAVQRWFVRRRGFASGLAV+GIGVGTLVMPPLA+ LI +GWR AY L V+A V+G G Sbjct: 126 VGAVQRWFVRRRGFASGLAVAGIGVGTLVMPPLASALIAQVGWRGAYFALAVLAVVLGAG 185 Query: 191 ASLLIADDPRRYGTGPDGDPLDTVS--------------------GAPLRQ-------GV 223 SLLI +DPR G PDGD D + AP R G Sbjct: 186 MSLLIENDPRERGLLPDGDVTDPAADGASASARDRGPCAKRAPSDAAPRRSPTDVPAAGA 245 Query: 224 SIRAAVRTARFAGLYAACLVSAFGVFVPFVHLVPFAVDHQVAPTLAVLLLGMIGVGSTAG 283 ++R AV + FA LYAACLV +FGVFVPFVHLVP+AVDH VAP AVLLLG IGVGSTAG Sbjct: 246 TVREAVMSRPFASLYAACLVCSFGVFVPFVHLVPYAVDHGVAPAAAVLLLGAIGVGSTAG 305 Query: 284 RFFLGGIADRVGRDAFLVAMYLGMAVSLAIWAIAGTFWSLTVFALLFGLFYGGWVAILPA 343 RFFLGG+ADR GR A L+AM+ GMAV+L WA AGT +L FAL+FG+FYGGWVA+LPA Sbjct: 306 RFFLGGLADRFGRRASLLAMFAGMAVALVAWAGAGTVATLAAFALVFGVFYGGWVAVLPA 365 Query: 344 VVTDHFGGRNVGGIIGILYTSVAVGTLLGPSAAGFAYDIGHSYFIP 389 VV D FGGRNV IIG+LYTSVA GTL+GP+AAGF YD G Y +P Sbjct: 366 VVMDAFGGRNVSAIIGVLYTSVAFGTLIGPAAAGFIYDAGGGYLVP 411