BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0021.p (261 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772471.1| hydratase [Bradyrhizobium japonicum USDA 110] >... 413 e-113 ref|YP_001206955.1| putative hydratase/decarboxylase 4-oxalocrot... 345 5e-93 ref|YP_001241380.1| putative hydratase/decarboxylase [Bradyrhizo... 332 2e-89 ref|ZP_02187273.1| sulfate adenylyltransferase subunit 2 [alpha ... 236 2e-60 ref|ZP_02191457.1| putative hydratase [alpha proteobacterium BAL... 145 5e-33 >ref|NP_772471.1| hydratase [Bradyrhizobium japonicum USDA 110] dbj|BAC51096.1| bll5831 [Bradyrhizobium japonicum USDA 110] Length = 259 Score = 413 bits (1062), Expect = e-113, Method: Compositional matrix adjust. Identities = 205/260 (78%), Positives = 226/260 (86%), Gaps = 2/260 (0%) Query: 1 MDRVLAAAKAIASARRSRAPLKALPKDVVPRDEAEGYQVQYALHALMQPHVGSLVGYKIG 60 MD +L AAKAIA ARR+ APL AL +V P DEAEGYQVQ ALH L+ PH G LVGYKIG Sbjct: 1 MDNILEAAKAIALARRNHAPLAAL--EVPPADEAEGYQVQRALHDLLLPHAGPLVGYKIG 58 Query: 61 CTSAVMQEYINIPHPCGGGVFEKVVHDSGVQLPAAEFVHVGVECEIAVRLARDLAPSEAP 120 CTS VMQ+YI IPHPC GGVF+K VHDSGV+L A+++V VGVECEIAVRL RDLA EAP Sbjct: 59 CTSQVMQDYIGIPHPCAGGVFQKGVHDSGVKLAASKYVRVGVECEIAVRLQRDLAAGEAP 118 Query: 121 FTAEWVAEAIDAYYPAIEIVDDRYVKWETLGAPTLIADDFFAAGCVLGEAVPRINMPDLR 180 FTAEWV EA+DAY+PAIEIVDDRYVKWET+GAPTLIADDFFAAGCVLG +VPR ++PDL+ Sbjct: 119 FTAEWVGEAVDAYHPAIEIVDDRYVKWETMGAPTLIADDFFAAGCVLGPSVPRSSVPDLK 178 Query: 181 AVAGRAIVNGKEEGRGTGADVLGHPHNALAWLANHLATEGRGLHAGQIVLTGSLVKTVWL 240 AV GRAIVNG+E G GTGADVLGHPHNALAWLANHLA EG+GLHAGQ+VLTGSLVKT+WL Sbjct: 179 AVKGRAIVNGEEVGHGTGADVLGHPHNALAWLANHLAAEGKGLHAGQLVLTGSLVKTLWL 238 Query: 241 KAGDTVVMELDGLGKVEATF 260 KAGD V MELDGLG VEA F Sbjct: 239 KAGDKVRMELDGLGVVEAEF 258 >ref|YP_001206955.1| putative hydratase/decarboxylase 4-oxalocrotonate decarboxylase [Bradyrhizobium sp. ORS 278] emb|CAL78738.1| putative hydratase/decarboxylase; putative 4-oxalocrotonate decarboxylase [Bradyrhizobium sp. ORS 278] Length = 263 Score = 345 bits (884), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 175/259 (67%), Positives = 200/259 (77%) Query: 2 DRVLAAAKAIASARRSRAPLKALPKDVVPRDEAEGYQVQYALHALMQPHVGSLVGYKIGC 61 D VLAAA++I +ARR+R PL LP + P+DEA GY++Q+ H L+ H G GYKIGC Sbjct: 4 DEVLAAAQSIVAARRARTPLAPLPAHLQPKDEAAGYEIQHEAHWLLAEHAGDFAGYKIGC 63 Query: 62 TSAVMQEYINIPHPCGGGVFEKVVHDSGVQLPAAEFVHVGVECEIAVRLARDLAPSEAPF 121 TS VMQ+YI IPHPC GGVF VH SGV L AA+FV VGVECEIAVRLA+DL P +APF Sbjct: 64 TSQVMQDYIGIPHPCAGGVFAGGVHASGVTLRAADFVRVGVECEIAVRLAKDLLPLDAPF 123 Query: 122 TAEWVAEAIDAYYPAIEIVDDRYVKWETLGAPTLIADDFFAAGCVLGEAVPRINMPDLRA 181 T V +AI + AIEIVDDRYV+WET+GAPTL+ADDFFAAGCVLG AVPR DL A Sbjct: 124 TIAHVGDAIGDCHAAIEIVDDRYVQWETMGAPTLVADDFFAAGCVLGPAVPREAAGDLAA 183 Query: 182 VAGRAIVNGKEEGRGTGADVLGHPHNALAWLANHLATEGRGLHAGQIVLTGSLVKTVWLK 241 V GRA VN G+G+GADVLGHP +ALAWLANHLA+ G GL AGQ+VLTGSLVKT+WLK Sbjct: 184 VQGRAFVNDALAGQGSGADVLGHPLHALAWLANHLASRGDGLEAGQLVLTGSLVKTLWLK 243 Query: 242 AGDTVVMELDGLGKVEATF 260 GD V MELDGLG VEA F Sbjct: 244 PGDRVRMELDGLGVVEAVF 262 >ref|YP_001241380.1| putative hydratase/decarboxylase [Bradyrhizobium sp. BTAi1] gb|ABQ37474.1| putative hydratase/decarboxylase [Bradyrhizobium sp. BTAi1] Length = 263 Score = 332 bits (852), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 167/241 (69%), Positives = 187/241 (77%) Query: 20 PLKALPKDVVPRDEAEGYQVQYALHALMQPHVGSLVGYKIGCTSAVMQEYINIPHPCGGG 79 PL ALP ++ P DEA GY VQ ALH + G+ VGYKIGCTS VMQ+Y+ IPHPC GG Sbjct: 22 PLGALPPELQPEDEAAGYAVQRALHEQVAVRQGARVGYKIGCTSKVMQDYLQIPHPCAGG 81 Query: 80 VFEKVVHDSGVQLPAAEFVHVGVECEIAVRLARDLAPSEAPFTAEWVAEAIDAYYPAIEI 139 VF VH SGV LP +V VGVECEIA RLAR+L P APFTAE VA A+ A +PAIE+ Sbjct: 82 VFATGVHSSGVSLPTTGYVRVGVECEIATRLARNLPPEAAPFTAERVAAAVGACHPAIEV 141 Query: 140 VDDRYVKWETLGAPTLIADDFFAAGCVLGEAVPRINMPDLRAVAGRAIVNGKEEGRGTGA 199 VDDRYV+WETLGAPTLIADDFFAAGCVLG AV +PDL V GRA++N + G GTGA Sbjct: 142 VDDRYVRWETLGAPTLIADDFFAAGCVLGAAVAHSALPDLLTVRGRAMINDEVAGEGTGA 201 Query: 200 DVLGHPHNALAWLANHLATEGRGLHAGQIVLTGSLVKTVWLKAGDTVVMELDGLGKVEAT 259 DVLGHP +ALAWLANHLA G+GL AG++VLTGSLVKTVWLKAGD V MELDGLG VEA Sbjct: 202 DVLGHPFHALAWLANHLAATGQGLKAGELVLTGSLVKTVWLKAGDRVRMELDGLGVVEAA 261 Query: 260 F 260 F Sbjct: 262 F 262 >ref|ZP_02187273.1| sulfate adenylyltransferase subunit 2 [alpha proteobacterium BAL199] gb|EDP65615.1| sulfate adenylyltransferase subunit 2 [alpha proteobacterium BAL199] Length = 265 Score = 236 bits (603), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 130/256 (50%), Positives = 161/256 (62%), Gaps = 2/256 (0%) Query: 7 AAKAIASARRSRAPLKALPKDVV-PRDEAEGYQVQYALHALMQPH-VGSLVGYKIGCTSA 64 AA +A AR S P +LP ++ P AEGY VQ ALHA+++ G VG+KIGCT+ Sbjct: 9 AAIFLAQARLSGPPTPSLPDNLTAPGTIAEGYAVQSALHAVLESAGFGPRVGHKIGCTTP 68 Query: 65 VMQEYINIPHPCGGGVFEKVVHDSGVQLPAAEFVHVGVECEIAVRLARDLAPSEAPFTAE 124 VMQ Y+ I HPC G VFE V D V+LP + + VGVECEIA RL RDL + E Sbjct: 69 VMQAYLGIDHPCAGEVFETTVFDRAVELPLSRYQRVGVECEIAARLGRDLPVRSGGHSRE 128 Query: 125 WVAEAIDAYYPAIEIVDDRYVKWETLGAPTLIADDFFAAGCVLGEAVPRINMPDLRAVAG 184 V+EA+D+ IE+VDDRY + L P L+ADDFF AG VLG AVP DL A+ G Sbjct: 129 SVSEAVDSLMAGIELVDDRYADYSALPPPVLVADDFFNAGVVLGTAVPDWRSLDLSAIEG 188 Query: 185 RAIVNGKEEGRGTGADVLGHPHNALAWLANHLATEGRGLHAGQIVLTGSLVKTVWLKAGD 244 V+G E GRG GAD+LGHP ALAWLA+H A G L AG+ V+ GS+VKTVW++A Sbjct: 189 VMTVDGTEVGRGFGADILGHPLAALAWLADHRAALGMPLRAGEFVMLGSVVKTVWIEAPA 248 Query: 245 TVVMELDGLGKVEATF 260 V + GLG+ TF Sbjct: 249 KVGIRFAGLGEASVTF 264 >ref|ZP_02191457.1| putative hydratase [alpha proteobacterium BAL199] gb|EDP61814.1| putative hydratase [alpha proteobacterium BAL199] Length = 268 Score = 145 bits (367), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 97/259 (37%), Positives = 136/259 (52%), Gaps = 5/259 (1%) Query: 1 MDRVLAAAKAIASARRSRAPLKALPKDVVPRDEAEGYQVQYALHALMQPHVGSLVGYKIG 60 ++RV A +A +AR PL P + DEA Q + + G + GYK+ Sbjct: 7 IERVAEALEAQHAARARFEPLTG-PLALDTLDEAYAAQ-RVLIQRWKDGARGPIAGYKVA 64 Query: 61 CTSAVMQEYINIPHPCGGGVFEKVVHDSGVQLPAAEFVHVGVECEIAVRLARDLAPSEAP 120 TS +QE + HPC G +FE V+DS ++P A+FV +G+E E+ +R+ L P Sbjct: 65 LTSKAVQELCGVDHPCVGAIFESTVYDSPARIPRADFVRLGLEFELCLRIGTSLPGGPQP 124 Query: 121 FTAEWVAEAIDAYYPAIEIVDDRYVKWETLGAPTLIADDFFAAGCVLGEAVPRINMPDLR 180 +TAE V +AI PA E+V+DR + L A +L+AD+ + G V G A DL Sbjct: 125 YTAETVRDAIAEAVPAFELVEDRASDYSKLNAISLVADNCWCGGIVTGAATGDWRALDLT 184 Query: 181 AVAGRA--IVNGKEEGR-GTGADVLGHPHNALAWLANHLATEGRGLHAGQIVLTGSLVKT 237 R GK E T VL P N LAW+AN LA +GR L AG V+TGS ++T Sbjct: 185 QTPVRLEYTSGGKAETETATTGAVLDSPLNVLAWVANVLAGQGRPLEAGMWVMTGSALRT 244 Query: 238 VWLKAGDTVVMELDGLGKV 256 + +AGD V ++GLG V Sbjct: 245 RFAEAGDRAVYTIEGLGTV 263