BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0182.0061.p (402 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_425570.1| radical SAM family protein [Rhodospirillum rubr... 340 2e-91 ref|ZP_05091411.1| radical SAM domain protein [Carboxydibrachium... 148 2e-33 ref|ZP_08213068.1| Radical SAM domain protein [Thermoanaerobacte... 144 3e-32 ref|ZP_07268094.1| radical SAM domain protein [Finegoldia magna ... 143 4e-32 ref|ZP_07320756.1| radical SAM domain protein [Finegoldia magna ... 143 7e-32 >ref|YP_425570.1| radical SAM family protein [Rhodospirillum rubrum ATCC 11170] gb|ABC21283.1| Radical SAM [Rhodospirillum rubrum ATCC 11170] gb|AEO46961.1| radical SAM family protein [Rhodospirillum rubrum F11] Length = 448 Score = 340 bits (872), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 168/342 (49%), Positives = 225/342 (65%), Gaps = 3/342 (0%) Query: 1 MTAAVRLKPSAFNVKLRHEGRHLAFNSIIAYSLEMSEAQARVFNDALDEVAQSGSCKDEA 60 M A KPSAFN+ L + + FNS+ ++L + E + + L + + + Sbjct: 1 MFDAREWKPSAFNLLLEGKDGQILFNSVTGHALWLDEGRRAAIDQCLTGLGGDATALPD- 59 Query: 61 LLRCLYALGFVVPADHDELAVQRAVFQATKASTETLRFTIAPTMACNLHCSYCFQQDFAP 120 L L LGF VPA DE A+++A + A + L T+APTM CN+ CSYCFQQ+ Sbjct: 60 LAEALAKLGFAVPAQADEYALEKARYLAHRDDPRRLYLTLAPTMQCNMKCSYCFQQNVEH 119 Query: 121 CKTMRPDIERGIIEFARRKLEGCKTLVVQWFGGEPLLRYKRIVAMTEAFQRVCAERGVNY 180 + M P I +G++EFARRK E + +VVQWFGGEPL+ + I M+ Q +CA RG+ Y Sbjct: 120 SRAMTPAIAKGVVEFARRKAETAQGMVVQWFGGEPLMAWTTIQTMSAQLQAICAARGIGY 179 Query: 181 YAEILTNGVLLTPEIIANLPKLAVKAIQIPLDGDVDTYARRKGVSRERAQAYHDVLVDHM 240 YAE+LTNG+L+TPEI+A LPK+AV+A+QI LDG V TYA R+ V +RA+AY+ L+ HM Sbjct: 180 YAEMLTNGLLMTPEIVAALPKMAVRALQISLDGAVTTYAERRRVPLDRAEAYYRFLMAHM 239 Query: 241 HALVDATGSVTIRINVDRENPAAGYDVVRMFKRKGCVDPRIDFRLGFLNNRDGIVDCIPH 300 AL++ TGSVTIRINVDR+N DVVRMFKR +DPR+DFRLGF+N GI+DCIPH Sbjct: 240 PALIEGTGSVTIRINVDRQNITEAKDVVRMFKRHAILDPRLDFRLGFINTSRGIIDCIPH 299 Query: 301 DCLSDVEFSDTEAEFRRFLVEQGYRVXWRARAAQISMLCTVT 342 DC S+ EF+D E +FRRFL +QGY V + ++ CT T Sbjct: 300 DCFSNSEFADEEDDFRRFLKDQGYMVYGMPQP--VNHPCTAT 339 >ref|ZP_05091411.1| radical SAM domain protein [Carboxydibrachium pacificum DSM 12653] gb|EEB76721.1| radical SAM domain protein [Carboxydibrachium pacificum DSM 12653] Length = 441 Score = 148 bits (374), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 89/321 (27%), Positives = 168/321 (52%), Gaps = 11/321 (3%) Query: 7 LKPSAFNVKL---RHEGRHLAFNSIIAYSLEMSEAQARVFNDALDEVAQSGSCKDEALLR 63 +KPS +N + +A+NS ++ + + ++ + V + S D L+ Sbjct: 1 MKPSRYNFFFDFPEEPEKIVAYNSRTG---ALALMEKKNYDKYKNYVEKGISIDDSKLIE 57 Query: 64 CLYALGFVVPADHDELAVQRAVFQATKASTETLRFTIAPTMACNLHCSYCFQQDFAPCKT 123 L F++ + DEL + R ++ + + L TIAPT+ CN C YC+++D Sbjct: 58 DLKKGQFLIDDNVDELQLLRFNLWRSRFNDKNLGLTIAPTLGCNFVCVYCYEKDSQKNVF 117 Query: 124 MRPDIERGIIEFARRKLEGCKTLVVQWFGGEPLLRYKRIVAMTEAFQRVCAERGVNYYAE 183 M +++R IIE+ +R+++ +++ + W+GGEPLL + M+E ++C E V Y A Sbjct: 118 MSEEVQRKIIEYIKRQIKYLQSVNIVWYGGEPLLASDIVKDMSEKIIKLCDENEVIYGAS 177 Query: 184 ILTNGVLLTPEIIANLPKLAVKAIQIPLDGDVDTYARRKGVSRERAQAYHDVLVDHMHAL 243 I+TNG LT E++ L + IQI LDG D + +R+ + + Q + ++ ++ Sbjct: 178 IVTNGYNLTREVVEQFKNLRLSFIQITLDGPQDVHDKRRPL--KSGQGTFEKILTNIEEN 235 Query: 244 VDATGSVTIRINVDRENPAAGYDVVRMFKRKGCVDPRIDFRLGFLNNRDGIVDCIPHDCL 303 +D ++++RINVD+EN D++ +++G + R+ LG++ + +P+ CL Sbjct: 236 IDIMPNISLRINVDKENVNRVNDILDELEKRG-LKNRLSVYLGYIEPTNACY--VPNKCL 292 Query: 304 SDVEFSDTEAEFRRFLVEQGY 324 S E+S F + L E+G+ Sbjct: 293 SMHEYSKVTYMFEKVLKERGF 313 >ref|ZP_08213068.1| Radical SAM domain protein [Thermoanaerobacter ethanolicus JW 200] gb|EGD50877.1| Radical SAM domain protein [Thermoanaerobacter ethanolicus JW 200] Length = 441 Score = 144 bits (362), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 88/321 (27%), Positives = 166/321 (51%), Gaps = 11/321 (3%) Query: 7 LKPSAFNVKL---RHEGRHLAFNSIIAYSLEMSEAQARVFNDALDEVAQSGSCKDEALLR 63 +KPS +N + +A+NS ++ + + ++ + V + S D L+ Sbjct: 1 MKPSRYNFFFDFPEEPEKIVAYNSRTG---ALALMEKKNYDKYKNYVEKGISIDDSKLIE 57 Query: 64 CLYALGFVVPADHDELAVQRAVFQATKASTETLRFTIAPTMACNLHCSYCFQQDFAPCKT 123 L F++ + DEL + R ++ + + L TIAPT+ CN C YC+++D Sbjct: 58 DLKKGQFLIDDNVDELQLLRFNLWRSRFNDKNLGLTIAPTLGCNFACVYCYEKDSQKNVF 117 Query: 124 MRPDIERGIIEFARRKLEGCKTLVVQWFGGEPLLRYKRIVAMTEAFQRVCAERGVNYYAE 183 M +++R IIE+ +R+++ +++ + W+GGEPLL + + M+E ++C E V Y A Sbjct: 118 MSEEVQRKIIEYIKRQIKYLQSVNITWYGGEPLLAFDIVKDMSEKIIKLCDENEVIYGAS 177 Query: 184 ILTNGVLLTPEIIANLPKLAVKAIQIPLDGDVDTYARRKGVSRERAQAYHDVLVDHMHAL 243 I+TNG LT E++ L + IQI LDG D + +R+ + + Q + ++ ++ Sbjct: 178 IVTNGYNLTREVVEQFKNLRLSFIQITLDGPQDVHDKRRPL--KGGQGTFEKILINIEEN 235 Query: 244 VDATGSVTIRINVDRENPAAGYDVVRMFKRKGCVDPRIDFRLGFLNNRDGIVDCIPHDCL 303 +D ++ +RINVD+EN ++ + +G + R+ LG + + + +P+ CL Sbjct: 236 IDIMPNIYLRINVDKENVTRVNKILDELEIRG-LKNRLSVYLGHVEHTND--SYVPNKCL 292 Query: 304 SDVEFSDTEAEFRRFLVEQGY 324 S E+S F + L E+G+ Sbjct: 293 SMYEYSKVTYMFEKVLKERGF 313 >ref|ZP_07268094.1| radical SAM domain protein [Finegoldia magna ACS-171-V-Col3] gb|EFK94512.1| radical SAM domain protein [Finegoldia magna ACS-171-V-Col3] Length = 427 Score = 143 bits (361), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 77/251 (30%), Positives = 130/251 (51%), Gaps = 5/251 (1%) Query: 65 LYALGFVVPADHDELAVQRAVFQATKASTETLRFTIAPTMACNLHCSYCFQQDFAPCKTM 124 L + FV+ D DEL+ + +++ + L TIAPT+ACN +CSYC++++ K+M Sbjct: 53 LKDMNFVLDDDDDELSKIKYELLSSRFNNSNLSLTIAPTLACNFNCSYCYEKNAENKKSM 112 Query: 125 RPDIERGIIEFARRKLEGCKTLVVQWFGGEPLLRYKRIVAMTEAFQRVCAERGVNYYAEI 184 E IIEF ++E TL + W+GGEPLL + I +++ ++C E V Y A I Sbjct: 113 NESTENSIIEFISEQIERLNTLSISWYGGEPLLEFDSIKRLSDKIIKMCDEYNVMYTAFI 172 Query: 185 LTNGVLLTPEIIANLPKLAVKAIQIPLDGDVDTYARRKGVSRERAQAYHDVLVDHMHALV 244 +TNG LLT EI+ L + +IQ+ LDG + + +++ + +++++ ++ Sbjct: 173 VTNGYLLTREIVEQFNSLKINSIQVTLDGSKEWHDKKRIL--HNGNGTYEIILKNLKDSY 230 Query: 245 DATGSVTIRINVDRENPAAGYDVVRMFKRKGCVDPRIDFRLGFLNNRDGIVDCIPHDCLS 304 + S IRIN+D +N + V+ K+ D + + N D DC C+ Sbjct: 231 EFLPSTHIRINLDNDNKNSYNQVIDDIKQFDKEDKMLIYIAPVENENDSYEDC---HCIQ 287 Query: 305 DVEFSDTEAEF 315 EF + EF Sbjct: 288 KSEFFEKMLEF 298 >ref|ZP_07320756.1| radical SAM domain protein [Finegoldia magna BVS033A4] gb|EFL54411.1| radical SAM domain protein [Finegoldia magna BVS033A4] Length = 427 Score = 143 bits (360), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 77/251 (30%), Positives = 130/251 (51%), Gaps = 5/251 (1%) Query: 65 LYALGFVVPADHDELAVQRAVFQATKASTETLRFTIAPTMACNLHCSYCFQQDFAPCKTM 124 L + FV+ D DEL+ + +++ + L TIAPT+ACN +CSYC++++ K+M Sbjct: 53 LKDMNFVLDDDDDELSRIKYELLSSRFNNSNLSLTIAPTLACNFNCSYCYEKNAENKKSM 112 Query: 125 RPDIERGIIEFARRKLEGCKTLVVQWFGGEPLLRYKRIVAMTEAFQRVCAERGVNYYAEI 184 E IIEF ++E TL + W+GGEPLL + I +++ ++C E V Y A I Sbjct: 113 NESTENSIIEFISEQIERLNTLSISWYGGEPLLEFDSIKRLSDKIIKMCDEYNVMYTAFI 172 Query: 185 LTNGVLLTPEIIANLPKLAVKAIQIPLDGDVDTYARRKGVSRERAQAYHDVLVDHMHALV 244 +TNG LLT EI+ L + +IQ+ LDG + + +++ + +++++ ++ Sbjct: 173 VTNGYLLTREIVEQFNSLKINSIQVTLDGSKEWHDKKRIL--HNGNGTYEIILKNLKDSY 230 Query: 245 DATGSVTIRINVDRENPAAGYDVVRMFKRKGCVDPRIDFRLGFLNNRDGIVDCIPHDCLS 304 + S IRIN+D +N + V+ K+ D + + N D DC C+ Sbjct: 231 EFLPSTHIRINLDNDNKNSYNQVIDDIKQFDKEDKMLIYIAPVENENDSYEDC---HCIQ 287 Query: 305 DVEFSDTEAEF 315 EF + EF Sbjct: 288 KSEFFEKMLEF 298