BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0182.0060.p (497 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_425571.1| radical SAM family protein [Rhodospirillum rubr... 580 e-163 ref|YP_003023235.1| radical SAM protein [Geobacter sp. M21] >gi|... 223 7e-56 ref|YP_002137626.1| radical SAM protein [Geobacter bemidjiensis ... 220 5e-55 ref|NP_618077.1| moaA/nifB/pqqE family protein [Methanosarcina a... 217 5e-54 ref|YP_004197464.1| Radical SAM domain-containing protein [Geoba... 196 1e-47 >ref|YP_425571.1| radical SAM family protein [Rhodospirillum rubrum ATCC 11170] gb|ABC21284.1| Radical SAM [Rhodospirillum rubrum ATCC 11170] gb|AEO46962.1| radical SAM family protein [Rhodospirillum rubrum F11] Length = 500 Score = 580 bits (1494), Expect = e-163, Method: Compositional matrix adjust. Identities = 273/496 (55%), Positives = 347/496 (69%) Query: 2 LNTSGSQYPAVRCEFILKVDGARPIVYSLDYINTQIYYLAPIEGFALSLLDGQRSISELS 61 + Q+ A+R EF+ K+DG RPI+Y LDYINT I+ L PI+GF LSLLDG+ S+L Sbjct: 5 FTIAAEQFAALRREFVFKIDGDRPILYPLDYINTHIHILQPIQGFVLSLLDGRTRFSDLD 64 Query: 62 VLFKSLLPRTDKPLLDVLRSADTLVRTSPSQTGLGKDGLFDISDEPIADAQSFDPREFII 121 +F++L P + PLLD+L S DTLVR S G+G+DGL ++SD PI A FD R F++ Sbjct: 65 RMFRALFPESPLPLLDLLASVDTLVRNKVSMAGIGRDGLIEVSDAPIPHAPRFDSRHFVV 124 Query: 122 DPAHFAARKNDPKTSLRLDTPINVYTVFTHRCLTDCVYCYADRCKMTEMPISRWRQIIRE 181 F D K RL PINVYTV THRC TDC+YCYADR K+ EM +SRW++I+ E Sbjct: 125 GGDDFRTVMGDVKQRFRLRAPINVYTVTTHRCHTDCLYCYADRKKLPEMALSRWQEILAE 184 Query: 182 MRSLGIWLASPDNGDTFARRDGIDLLECLLENEMHFLLSTKAYVSKDYIKRLIDAGFTKK 241 M LG+ L SPDNGDTFAR+DG+D++E +L N+MHFLLSTK ++SK I RL++AG +K Sbjct: 185 MAGLGVRLCSPDNGDTFARKDGVDIIESMLRNDMHFLLSTKIFLSKPTIGRLMEAGLGEK 244 Query: 242 VRGVIQRQVQLSIDAVDDDVSRRVLCIKRSNVARNQETIENFLSFGIMPKIKAVITGLNH 301 V GVI R VQLSID+VD+ + R+L + + +N ET NF+SFGIMP +K V+TGLN Sbjct: 245 VNGVIDRPVQLSIDSVDEALGMRLLNVTKPRHQKNVETFNNFMSFGIMPIVKCVVTGLNA 304 Query: 302 DQPEKIVHRFYERGARVFHFVRYGRSFHRHTDQLFVDEVAIASLRDQFDRIRSRYDDADI 361 +QP IV +FY G R F FVRY RS HRHTD LF+ +L QF IR R+ D + Sbjct: 305 NQPLPIVEQFYALGGRRFSFVRYTRSLHRHTDDLFLGPQHAETLTRQFAEIRERFPDIVL 364 Query: 362 VENLTQAPPDQSELDANAARAIWDGRIGCGGGWSALGISPSGKAFLCEQMKMADPFFVGD 421 EN++ + L A + +WDGRIGCGGGW +LGI P GKAFLCEQM +P+FVGD Sbjct: 365 NENVSLSAGGPIVLTAELKQRLWDGRIGCGGGWHSLGIGPDGKAFLCEQMAYEEPYFVGD 424 Query: 422 ASYQSIEEIWHGAHLKRFIYPEQMQFAGTACESCPEFETCMWEKGRCYRDAYFSYGTVFH 481 AS Q+I EIW+ + FI+P + QFAGT CE+CP FE+CMWEKGRCYRDAY+SYGT+F Sbjct: 425 ASRQTIAEIWNSDAMMAFIHPSRDQFAGTTCETCPGFESCMWEKGRCYRDAYYSYGTIFT 484 Query: 482 PPPLCPSNYRSGLRLS 497 PPLCP+N R G+RL Sbjct: 485 APPLCPNNERPGVRLG 500 >ref|YP_003023235.1| radical SAM protein [Geobacter sp. M21] gb|ACT19477.1| Radical SAM domain protein [Geobacter sp. M21] Length = 511 Score = 223 bits (568), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 131/477 (27%), Positives = 234/477 (49%), Gaps = 38/477 (7%) Query: 40 LAPIEGFALSLLDGQRSISELSVLFKSLLPRTDKPLLDVLRSADTLVRTSPSQTGLGKDG 99 ++P+ G LSL+DG+ + L ++ + D L +A+ V T D Sbjct: 50 VSPLVGATLSLMDGRLAFRHLCLIVQYANGFED------LEAAEEFVTKVILATNKECDA 103 Query: 100 LFDISDEPIADAQSFDPREFIIDPAHFAARKNDPKTSLRLDTPINVYTVFTHRCLTDCVY 159 + +++ E +P +DP FA+ + + R P+++ +F++ C T+C Y Sbjct: 104 VVEMTPE-------LEPFVIKMDPLRFASNTSVSQEQKRPAAPLSLNLMFSNECETNCSY 156 Query: 160 CYADRCKMTE---MPISRWRQIIREMRSLGIWLASPDNGDTFARRDGIDLLECLLENEMH 216 C A R + E + RW++II E +SLGI + GD R++ + L+ L+ +M Sbjct: 157 CQAQRRYLPENTLLSAKRWKEIISEAKSLGIEQVTLSGGDPLYRKEALVLIGELIAKKML 216 Query: 217 FLLSTKAYVSKDYIKRLIDAGFTKKVRGVIQRQVQLSIDAVDDDVSRRVLCIKRSNVARN 276 F L TK ++++ RL++ G TK + + R++QL++D D++ S + K S+ +++ Sbjct: 217 FQLPTKCCITEEIADRLVEVGMTKPINHYL-REIQLTMDGPDEEPSHKSAG-KSSHFSKS 274 Query: 277 QETIENFLSFGIMPKIKAVITGLNHDQPEKIVHRFYERGARVFHFVRYGRSFHRHTDQLF 336 ++I N + G +IKA+ T +N Q + + + E G Y +++H+H L Sbjct: 275 VQSIRNLQARGFNLRIKALATSVNASQIYQWIKQLVEMGVTQITVAAYAKAYHKHNGNLS 334 Query: 337 VDEVAIASLRDQFDRIRSRYDDADIVEN----------------LTQAPPDQSELD---- 376 + S+ Q + R+ + + N + Q P + +D Sbjct: 335 LSSQDKISIAQQCQKARTDFPGISLSTNGIGPAPGAEQNTESASICQRGPIEISVDIGEE 394 Query: 377 ANAARAIWDGRIGCGGGWSALGISPSGKAFLCEQMKMADPFFVGDASYQSIEEIWHGAHL 436 ++ + W R+ C GG +++ I+P GK LC+ + +PFFVGD S QS+ E+W+ L Sbjct: 395 SSGKISYWKSRVQCAGGRTSMTITPDGKVVLCDTVPQDEPFFVGDVSTQSLLEVWNSESL 454 Query: 437 KRFIYPEQMQFAGTACESCPEFETCMWEKGRCYRDAYFSYGTVFHPPPLCPSNYRSG 493 F YP + +F G+AC C + E C + G C+RD+YF+ GT+F P CP R Sbjct: 455 LNFAYPPREKFKGSACYDCDKLEECQSKLGHCFRDSYFNLGTIFGPAAHCPMAQRQA 511 >ref|YP_002137626.1| radical SAM protein [Geobacter bemidjiensis Bem] gb|ACH37830.1| radical SAM domain iron-sulfur cluster-binding oxidoreductase [Geobacter bemidjiensis Bem] Length = 507 Score = 220 bits (560), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 129/475 (27%), Positives = 232/475 (48%), Gaps = 42/475 (8%) Query: 40 LAPIEGFALSLLDGQRSISELSVLFKSLLPRTDKPLLDVLRSADTLVRTSPSQTGLGKDG 99 ++P+ G LSL+DG+ + L ++ + + L +A V T D Sbjct: 50 VSPLVGATLSLMDGRLAFRHLCLIVQYAQG------FENLEAAKEFVTKVILATNKECDA 103 Query: 100 LFDISDEPIADAQSFDPREFIIDPAHFAARKNDPKTSLRLDTPINVYTVFTHRCLTDCVY 159 + D++ E +P +DP FA+ + P+ R P+++ +F++ C T+C Y Sbjct: 104 VVDMTPE-------LEPFAVKMDPLRFASNMSGPQEQKRPAAPLSLNLMFSNECETNCSY 156 Query: 160 CYADRCKMTE---MPISRWRQIIREMRSLGIWLASPDNGDTFARRDGIDLLECLLENEMH 216 C A R + E +P RW++II E +SLGI + GD R++ + L+ L+ +M Sbjct: 157 CQAQRRYLPENTLLPAKRWKEIIGEAKSLGIEQVTLSGGDPLYRKEALALIGELIAKDML 216 Query: 217 FLLSTKAYVSKDYIKRLIDAGFTKKVRGVIQRQVQLSIDAVDDDVSRRVLCIKRSNVARN 276 F L TK ++++ +RL++ G T + + R++QL+ID D+ + K +V+++ Sbjct: 217 FQLPTKCCITEEIAERLVEVGMTNPINQYL-REIQLTIDGPDEGPAH-----KSGHVSKS 270 Query: 277 QETIENFLSFGIMPKIKAVITGLNHDQPEKIVHRFYERGARVFHFVRYGRSFHRHTDQLF 336 ++I N + G ++KAV T +N + + + E G Y + +H+H L Sbjct: 271 VQSIRNLKARGFNFRVKAVATPVNASRIYPWIKQLVEMGVTQITVAAYAKVYHKHNGNLS 330 Query: 337 VDEVAIASLRDQFDRIRSRYDDADI--------------VENLTQAPPDQSEL------D 376 + S+ Q + RS + + + +E+ + P E+ + Sbjct: 331 LSSHDKFSIAQQCQKARSDFPEISLSTSGIGPAPGAEQCMESASVFKPGPIEISVDIGDE 390 Query: 377 ANAARAIWDGRIGCGGGWSALGISPSGKAFLCEQMKMADPFFVGDASYQSIEEIWHGAHL 436 ++ + W R C GG +++ ++P GK LC+ + + FFVGD S QS+ E+W+ L Sbjct: 391 SSGKISYWKSRTQCAGGRTSMTVTPDGKVVLCDTVPQDESFFVGDVSTQSLLEVWNSECL 450 Query: 437 KRFIYPEQMQFAGTACESCPEFETCMWEKGRCYRDAYFSYGTVFHPPPLCPSNYR 491 F YP + +F G+AC C + E C + G C+RD+YF++GT+F P CP R Sbjct: 451 LNFAYPSREKFKGSACYDCDKLEECQSKAGHCFRDSYFNFGTIFGPAANCPVAQR 505 >ref|NP_618077.1| moaA/nifB/pqqE family protein [Methanosarcina acetivorans C2A] gb|AAM06557.1| moaA/nifB/pqqE family protein [Methanosarcina acetivorans C2A] Length = 487 Score = 217 bits (552), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 121/363 (33%), Positives = 193/363 (53%), Gaps = 12/363 (3%) Query: 137 LRLDTPINVYTVFTHRCLTDCVYCYADRCKMTEMPISRWRQIIREMRSLGIWLASPDNGD 196 +RL P++V FT+RC DC+YCYA+R K E +S+W +I E++ I+L D Sbjct: 131 VRLSRPLSVSISFTNRCHYDCIYCYAERKKCEEKELSQWSRIFDELQENEIFLVDIGGTD 190 Query: 197 TFARRDGIDLLECLLENEMHFLLSTKAYVSKDYIKRL--IDAGFTKKVRGVIQRQVQLSI 254 FAR D +++L+ +++ + FLLSTK+++ ++ K L + G + + R VQLSI Sbjct: 191 IFARPDALEILQAMVDRDFVFLLSTKSFIDENTAKCLASMHIGIGDEPEHLF-RYVQLSI 249 Query: 255 DAVDDDVSRRVLCIKRSNVARNQETIENFLSFGIMPKIKAVITGLNHDQPEKIVHRFYER 314 D+VD + ++ K+ + + ++++N + GI PKIK V+T N+ + IV F Sbjct: 250 DSVDAPTAGYMV-KKKDHYEKTIQSVKNLMKVGICPKIKCVLTPFNYLEMSAIVDEFANL 308 Query: 315 GARVFHFVRYGRSFHRHTDQLFVDEVAIASLRDQFDRIRSRYD--DADIVENLTQAPPDQ 372 G R F FV+Y RS +RH + LF+ + + + + Y + + +NLT Sbjct: 309 GVRDFQFVQYSRSKYRHNNDLFLSSKHKEFISNFAEYVNQNYPLLNISVEQNLTTGG--- 365 Query: 373 SELDANAARAIWDGRIGCGGGWSALGISPSGKAFLCEQMKMADPFFVGDASYQSIEEIWH 432 + N + W R C GG S + I P+G LCEQ+ + F VG+ + I +W+ Sbjct: 366 ---NRNLSPEKWKDRAVCSGGRSNMIIQPNGDVTLCEQIPHREEFIVGNVFDEGIMGVWN 422 Query: 433 GAHLKRFIYPEQMQFAGTACESCPEFETCMWEKGRCYRDAYFSYGTVFHPPPLCPSNYRS 492 + FIYP + +F + C C EFE C KG CYRD SYGT++ P CP + Sbjct: 423 SKKITDFIYPPREKFKNSVCYDCLEFEACHRIKGYCYRDVLSSYGTIYDAQPECPVQTKL 482 Query: 493 GLR 495 +R Sbjct: 483 PVR 485 >ref|YP_004197464.1| Radical SAM domain-containing protein [Geobacter sp. M18] gb|ADW12188.1| Radical SAM domain protein [Geobacter sp. M18] Length = 506 Score = 196 bits (497), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 131/469 (27%), Positives = 225/469 (47%), Gaps = 39/469 (8%) Query: 40 LAPIEGFALSLLDGQRSISELSVLFKSLLPRTDKPLLDVLRSADTLVRTSPSQTGLGKDG 99 L+P G L+L+DG+ ++ LS++ + + +S + L R D Sbjct: 50 LSPKMGATLALMDGRMTLRHLSMIAQYAFDLESQE-----KSQEFLTRVI-MVANREDDA 103 Query: 100 LFDISDEPIADAQSFDPREFIIDPAHFAARKNDPKTSLRLDTPINVYTVFTHRCLTDCVY 159 + +++ E + DP EF++ + + A++ PI++ +F++ C T+C Y Sbjct: 104 VVNMTPELEPYVKRIDPSEFVVKSSKWKAQQMPA-------APISLNLMFSNDCHTNCAY 156 Query: 160 CYAD-RC--KMTEMPISRWRQIIREMRSLGIWLASPDNGDTFARRDGIDLLECLLENEMH 216 C+A C + ++ RW++++RE +SLGI + GD R+D + L+E L++ ++ Sbjct: 157 CFAKGHCVPESKQLSTGRWKELLREAKSLGIEQVTLSGGDPLFRKDALKLIEELIDLDLL 216 Query: 217 FLLSTKAYVSKDYIKRLIDAGFTKKVRGVIQRQVQLSIDAVDDDVSRRVLCIKRSNVARN 276 F LSTK Y++++ +L+ G T+ V + R++QL++D D+ + L R Sbjct: 217 FALSTKCYITEEIADKLVAIGMTQPVNQYV-REIQLNMDP--DEPTADKLAGSPGYYNRA 273 Query: 277 QETIENFLSFGIMPKIKAVITGLNHDQPEKIVHRFYERGARVFHFVRYGRSFHRHTDQLF 336 +I N L G ++KAV+T L + V + G R Y R+ H D LF Sbjct: 274 VVSIRNLLKRGFNVRVKAVVTALTAPYIYEWVEELQDMGVRQISVAAYNRNVHNQNDGLF 333 Query: 337 VDEVAIASLRDQFDRIRSRYDDADIVE------NLTQA------------PPDQSELDAN 378 + + S+ DQ R + + D+ N T A P + +E+ Sbjct: 334 LTKEDRGSILDQCRHARIDFPEIDLRTIGFESGNDTVAMTRMQSADPVALPDEDNEISEK 393 Query: 379 AARAIWDGRIGCGGGWSALGISPSGKAFLCEQMKMADPFFVGDASYQSIEEIWHGAHLKR 438 R +GR G S++ I+P GK L + + + + FVGD S SI ++W+ L+ Sbjct: 394 IRRWKDEGRSFVGH--SSMTITPDGKVMLYDTVPLDEALFVGDVSRSSIVDVWNSEPLQN 451 Query: 439 FIYPEQMQFAGTACESCPEFETCMWEKGRCYRDAYFSYGTVFHPPPLCP 487 + +P+ QF G AC C + C + G C+RDAYF GTVF PP CP Sbjct: 452 YAFPKTEQFKGAACYECKDLAKCQRKAGYCFRDAYFQSGTVFVPPAQCP 500