BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0181.0008.p (385 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_768838.1| hypothetical protein bll2198 [Bradyrhizobium ja... 634 e-179 ref|YP_001242364.1| putative glutathionylspermidine synthase fam... 593 e-167 ref|YP_487519.1| glutathionylspermidine synthase [Rhodopseudomon... 582 e-164 ref|YP_001203610.1| putative glutathionylspermidine synthase fam... 578 e-163 ref|YP_002965988.1| synthetase/amidase [methylobacterium extorqu... 455 e-126 >ref|NP_768838.1| hypothetical protein bll2198 [Bradyrhizobium japonicum USDA 110] dbj|BAC47463.1| bll2198 [Bradyrhizobium japonicum USDA 110] Length = 386 Score = 634 bits (1634), Expect = e-179, Method: Compositional matrix adjust. Identities = 308/386 (79%), Positives = 334/386 (86%), Gaps = 1/386 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M+RI C ERDDWR TA GF FHTIDGERYWDERAYYAFTLDEIER IE PTGEIDAMC Sbjct: 1 MRRIICLERDDWRQTAAQCGFVFHTIDGERYWDERAYYAFTLDEIERGIETPTGEIDAMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 LELAGR + DER+L+RLKIP+AFW LIA SW R + SLYGRLDL FD +GPAKLLEYNAD Sbjct: 61 LELAGRTIGDERHLQRLKIPEAFWSLIAESWERDDRSLYGRLDLKFDGKGPAKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTSIFEAAVFQWTWLEQAI+R IIP RADQFNSIH+RLIEAWK IGA +HLTG TGN Sbjct: 121 TPTSIFEAAVFQWTWLEQAIERRIIPVRADQFNSIHERLIEAWKEIGAGRHVHLTGTTGN 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDD-GQFVDLDERPIALAFKLYPWEWMFH 239 EDAGTLAYL+DTA QAGL TTLLDIE IG RD+ G FVDLD+R IALAFKLYPWEWMFH Sbjct: 181 EEDAGTLAYLEDTARQAGLSTTLLDIEAIGWRDEAGGFVDLDDRDIALAFKLYPWEWMFH 240 Query: 240 DAFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSF 299 DAFG L APT+WIEPPWKA+LSNKGILPLLWEMFPNHPNLLPA+FEDD +AA+LG+S+ Sbjct: 241 DAFGAKLEGAPTRWIEPPWKAVLSNKGILPLLWEMFPNHPNLLPAFFEDDVRAAELGSSY 300 Query: 300 VRKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 VRKP+ SREGANV LVS G L GPYG EGF+RQAL+PLPNFSG YPV+GSWLV+ Sbjct: 301 VRKPLLSREGANVTLVSGGTPLDEHAGPYGAEGFVRQALSPLPNFSGFYPVIGSWLVNHE 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAIL 385 PCGLSIREDE+PITGN SRFLPHAIL Sbjct: 361 PCGLSIREDESPITGNGSRFLPHAIL 386 >ref|YP_001242364.1| putative glutathionylspermidine synthase family protein [Bradyrhizobium sp. BTAi1] gb|ABQ38458.1| Putative Glutathionylspermidine synthase family protein [Bradyrhizobium sp. BTAi1] Length = 386 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 285/386 (73%), Positives = 318/386 (82%), Gaps = 1/386 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 MQRI CPERDDWR TA GF FH +DGERYWDERAYYAFTL EIE IE PTGEI+ MC Sbjct: 1 MQRIPCPERDDWRETAARCGFDFHELDGERYWDERAYYAFTLAEIELGIEEPTGEIEQMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDA-RGPAKLLEYNA 119 LEL R V+DE LRRLKIPD FW L+A+SW+RK+ SLYGRLDL+F R PAKLLEYN+ Sbjct: 61 LELVSRVVKDEVLLRRLKIPDRFWPLLASSWKRKDDSLYGRLDLSFQGGRAPAKLLEYNS 120 Query: 120 DTPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTG 179 DTPTSIFEAAVFQWTWLEQAI+R I+P RADQ+NSIH+RLIE W+ IG PLHL GLT Sbjct: 121 DTPTSIFEAAVFQWTWLEQAIERRILPGRADQYNSIHERLIERWRLIGKGRPLHLAGLTD 180 Query: 180 NPEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFH 239 EDA TLAYL DTA QAGL TT+LD+ +IG R+ G FVDLD + +ALAFKLYPWEWMF Sbjct: 181 AAEDAATLAYLADTATQAGLSTTILDMADIGWREAGGFVDLDNKDMALAFKLYPWEWMFQ 240 Query: 240 DAFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSF 299 DAFG L+ A TQW+EPPWKA+LSNKGILPLLW+MF HPNLLPAYFEDDP+AA LG SF Sbjct: 241 DAFGAKLAIALTQWVEPPWKAVLSNKGILPLLWQMFEGHPNLLPAYFEDDPKAASLGPSF 300 Query: 300 VRKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 VRKPIYSREGANV LV+ G+ + ++GPYG EGFIRQALAPLP+F G +PV+GSWLV Sbjct: 301 VRKPIYSREGANVSLVAEGIAVSEQEGPYGAEGFIRQALAPLPDFGGVFPVVGSWLVAHA 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAIL 385 PCGLSIRED PITGN SRFLPHAIL Sbjct: 361 PCGLSIREDVTPITGNRSRFLPHAIL 386 >ref|YP_487519.1| glutathionylspermidine synthase [Rhodopseudomonas palustris HaA2] gb|ABD08608.1| Glutathionylspermidine synthase [Rhodopseudomonas palustris HaA2] Length = 385 Score = 582 bits (1499), Expect = e-164, Method: Compositional matrix adjust. Identities = 276/385 (71%), Positives = 311/385 (80%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M+RIAC ERDDW TA GF FH+ G+RYWDER YYAFTLDEIER IEAPT E+DAMC Sbjct: 1 MRRIACAERDDWEATAAGCGFNFHSASGQRYWDERTYYAFTLDEIERQIEAPTAELDAMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 L+L RA+RDE LRRL IP+ +W IA SWRR +PSLYGRLDL FD R PAKLLEYNAD Sbjct: 61 LDLVDRAIRDEPMLRRLNIPEQYWSFIATSWRRGDPSLYGRLDLRFDGRDPAKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTS+FEAAVFQWTWLEQA R IIPKRADQFNS+H+RLI AW+R LHLTG + Sbjct: 121 TPTSLFEAAVFQWTWLEQATARQIIPKRADQFNSLHERLIAAWRRFAGGRRLHLTGTLSS 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 ED+GTL YL D A Q G++T L+DI +IG R G FVDL +R I LAFKLYPWEW+F + Sbjct: 181 AEDSGTLHYLMDVAQQGGVETVLIDIADIGWRSGGGFVDLQDRNIELAFKLYPWEWLFRE 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 AFG L+ A T W+EPPWKAILSNKGILPLLW+M P HPNLLPAYFEDDP+A QLG SFV Sbjct: 241 AFGPRLAEAQTGWMEPPWKAILSNKGILPLLWKMHPGHPNLLPAYFEDDPEAGQLGASFV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGTP 360 RKP+YSREGAN+ LV+ G AE GPYG EG+IRQA PLPNFSGQ+PVLGSWLVDG+P Sbjct: 301 RKPLYSREGANITLVNDGAIEAAEDGPYGAEGYIRQAADPLPNFSGQHPVLGSWLVDGSP 360 Query: 361 CGLSIREDENPITGNTSRFLPHAIL 385 CGLSIRED++ ITGNTSRF+PHAIL Sbjct: 361 CGLSIREDDSLITGNTSRFVPHAIL 385 >ref|YP_001203610.1| putative glutathionylspermidine synthase family protein [Bradyrhizobium sp. ORS 278] emb|CAL75373.1| Putative Glutathionylspermidine synthase family protein [Bradyrhizobium sp. ORS 278] Length = 391 Score = 578 bits (1489), Expect = e-163, Method: Compositional matrix adjust. Identities = 283/391 (72%), Positives = 312/391 (79%), Gaps = 6/391 (1%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 MQRI CPERDDWR TA+ GF F +DGERYWDERAYYAF+L EIE IE PT EI+ +C Sbjct: 1 MQRIPCPERDDWRQTAQQCGFDFCEVDGERYWDERAYYAFSLAEIEHGIEEPTAEIEQIC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDA-----RGPAKLL 115 LEL R V DE LRRL+IP A W ++ SW R++ SLYGRLDL+F R PAKLL Sbjct: 61 LELVARVVADETLLRRLQIPQAVWPFLSESWERRDGSLYGRLDLSFQGFRDGGRAPAKLL 120 Query: 116 EYNADTPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLT 175 EYNADTPTSIFEAAVFQWTWLEQAI+R I+P RADQ+NSIH+RLI W+ +G LHL Sbjct: 121 EYNADTPTSIFEAAVFQWTWLEQAIERRIVPGRADQYNSIHERLIARWRTLGEGRRLHLA 180 Query: 176 GLTGNPEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRD-DGQFVDLDERPIALAFKLYPW 234 GL + ED TLAYL DTAAQAGL T+LLDI EIG RD G FVDLD IALAFKLYPW Sbjct: 181 GLMEDAEDGATLAYLADTAAQAGLSTSLLDISEIGWRDTGGGFVDLDTNDIALAFKLYPW 240 Query: 235 EWMFHDAFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQ 294 EWMFHDAFG L+ APTQWIEPPWKAILSNKGILPLLW+MF HPNLLPAYFEDDP+AA Sbjct: 241 EWMFHDAFGAKLAGAPTQWIEPPWKAILSNKGILPLLWQMFEGHPNLLPAYFEDDPKAAS 300 Query: 295 LGTSFVRKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSW 354 LG SFVRKPIYSREGANV LVS GV++ A+ GPYG EGFIRQ LAPLP+F G +PV+GSW Sbjct: 301 LGPSFVRKPIYSREGANVSLVSDGVSVSAQDGPYGAEGFIRQGLAPLPDFGGVFPVIGSW 360 Query: 355 LVDGTPCGLSIREDENPITGNTSRFLPHAIL 385 LV PCGLSIRED +PITGN SRFLPHAIL Sbjct: 361 LVAHEPCGLSIREDASPITGNRSRFLPHAIL 391 >ref|YP_002965988.1| synthetase/amidase [methylobacterium extorquens AM1] gb|ACS42711.1| putative synthetase/amidase [Methylobacterium extorquens AM1] Length = 387 Score = 455 bits (1171), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/384 (56%), Positives = 265/384 (69%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M+R AC ER WR A +AGF FHTIDGE YWDE YAFTL EIE IE P+ E+ A+C Sbjct: 1 MRRFACGERPGWREIAGDAGFAFHTIDGEPYWDESHAYAFTLAEIEDDIEGPSAELHALC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 L+ A AV DER L L IP+ WD + ASW R++PSLYGRLD ++ GPAKLLEYNAD Sbjct: 61 LDFAAEAVADERILTSLAIPEPVWDTVRASWHRRDPSLYGRLDFSYGGTGPAKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPT+++E AVFQW WLEQ ++ +P +DQFN++H+RLI I + T Sbjct: 121 TPTALYETAVFQWLWLEQGLKEGRLPAGSDQFNALHERLIARLAEIAPPGLIAFAADTSG 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 PED GT YL+D A QAG T LLD+ +IGLR DG F DE+P A FKLYPWEW F D Sbjct: 181 PEDLGTAGYLQDCAVQAGCATILLDLAQIGLRADGAFCGPDEQPFAALFKLYPWEWAFQD 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 AFG ++++PT+++EPPWK +LSNKG+L LW P HPNLLPA+FE DP A LG +V Sbjct: 241 AFGSAVAASPTRFLEPPWKMVLSNKGLLAHLWAREPGHPNLLPAFFESDPAGASLGGDYV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGTP 360 RKP+ SREGANVE+ S G + GPYG GFIRQ LA LP F G+ P+LG+W+V P Sbjct: 301 RKPLLSREGANVEIFSDGARVAGADGPYGGNGFIRQGLAELPCFDGRRPLLGAWIVGDAP 360 Query: 361 CGLSIREDENPITGNTSRFLPHAI 384 GL IRE + PITG+T+ F+PH I Sbjct: 361 AGLCIRESDGPITGDTALFVPHLI 384