BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0177.0006.p (625 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_07027317.1| diguanylate cyclase/phosphodiesterase with PA... 588 e-165 ref|YP_001925587.1| GAF sensor-containing diguanylate cyclase/ph... 340 5e-91 ref|YP_001640236.1| diguanylate cyclase [Methylobacterium extorq... 339 8e-91 ref|YP_003069034.1| diguanylate cyclase/phosphodiesterase [Methy... 338 1e-90 ref|YP_002964000.1| diguanylate cyclase (GGDEF)/phosphodiesteras... 338 2e-90 >ref|ZP_07027317.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Afipia sp. 1NLS2] gb|EFI51073.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Afipia sp. 1NLS2] Length = 958 Score = 588 bits (1515), Expect = e-165, Method: Compositional matrix adjust. Identities = 314/522 (60%), Positives = 381/522 (72%), Gaps = 18/522 (3%) Query: 29 TKGSIRSQILIFCLAMSAVAVALGGYSILGIRHAGDLVAKTFDESLMSINYARAAGADFA 88 KG+IR+QILI CL MSA+ ALG Y+ GIRHA DLVAKTFDESLMSINYARAA ADF+ Sbjct: 11 VKGTIRTQILICCLLMSAITAALGVYATSGIRHAADLVAKTFDESLMSINYARAAAADFS 70 Query: 89 TMRVASSQRLLTTDPEIRANLDGQIEKLAKTLSEDLAIAADRSQSSRAAQAAAKVQEAAD 148 MR ASS+RL++TDP RA LD +I+ L KTL EDL IAA+RSQS+ A +A KVQEAAD Sbjct: 71 NMRAASSRRLVSTDPASRAALDKEIDSLGKTLHEDLNIAAERSQSTLAVASARKVQEAAD 130 Query: 149 AWMALHRRAVGSSTEGSAAADPREPGATVGDVDRYSKIVSQQVELLVNYTAGDGFLFRQK 208 AW ALH + V D + +D Y+K+V QQV+LL+NYTAGDGFLFRQ Sbjct: 131 AWTALHHKVVLEEDATDNPTDGKGQSTQAASLDNYAKVVDQQVDLLINYTAGDGFLFRQH 190 Query: 209 ALSTIKRDLQLNVAGLTVALFLSALFSWLLARRIIGPVAIASRAARSIAGGDLDATIPKG 268 ALS I RD+ N+ L AL LS SW L+RRIIGPVA+AS+AAR+IA G LD IP G Sbjct: 191 ALSAINRDINFNLVALAAALILSGFLSWRLSRRIIGPVAVASKAARNIAEGKLDVDIPAG 250 Query: 269 GTDELGTLLTAMETMRDNIRRMVNQEVSQRRSAQARLSDALETSREGVVLLDADGQVALA 328 G DELGTL+TAM MR+NIR M+ +E++QRRSAQARL+DALETS+EGVVLL ADGQVALA Sbjct: 251 GEDELGTLITAMGVMRNNIRDMMEREIAQRRSAQARLTDALETSQEGVVLLQADGQVALA 310 Query: 329 NSRASELIRLSPQLLQSNPLD-------AARLAPADGDIDGFASEAQLSDGRWLRVSRSE 381 NSRA E + +P LL+ +P + APA + ASEA+L +GRWLRVSRS Sbjct: 311 NSRACEFLAEAPDLLRCSPFEDQWIENPLVDFAPA--ALRDSASEARLPNGRWLRVSRSM 368 Query: 382 TQEGGVVLVYSDISALKQQKAELHETNLRLDAALTHMSQGLCLYNSEARLQVANRRLQVA 441 TQE GV++V+SDI+ALK Q+ +LHETN RLDAAL +MSQGLCLY+ +A RLQV Sbjct: 369 TQERGVIVVFSDITALKMQEEQLHETNRRLDAALENMSQGLCLYDKDA-------RLQVV 421 Query: 442 NRRFCEIFDISPELVIPGMTXE-VLDLSIAAGNHGERTVADLLAERERSMAQHD-GNYLQ 499 NRRFCEIF+I V GM+ E VL LS+ AGNH + V+DLLAER+R +++ +Y Q Sbjct: 422 NRRFCEIFNIPVGSVHAGMSFESVLMLSVMAGNHAGQNVSDLLAERQRFLSREKIDSYYQ 481 Query: 500 HLSDGRIIAIAQRPTSDGGWLVTCEDVTEQQRAESQIAFMXK 541 LSD R+I + RP SDGGWL TCEDVTEQ++AES+I FM + Sbjct: 482 QLSDDRVICVRHRPASDGGWLTTCEDVTEQRQAESKITFMAR 523 >ref|YP_001925587.1| GAF sensor-containing diguanylate cyclase/phosphodiesterase [Methylobacterium populi BJ001] gb|ACB81052.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Methylobacterium populi BJ001] Length = 950 Score = 340 bits (872), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 219/522 (41%), Positives = 317/522 (60%), Gaps = 33/522 (6%) Query: 32 SIRSQILIFCLAMSAVAVALGGYSILGIRHAGDLVAKTFDESLMSINYARAAGADFATMR 91 +IR +I + L +SA+ LGGY+ LGI G+LV KT+D SLMSINYARAA +D A ++ Sbjct: 26 TIRGRIFLAFLMLSAITAGLGGYAALGIMTTGELVDKTYDRSLMSINYARAAASDLAALQ 85 Query: 92 VASSQRLLTTDPEIRANLDGQIEKLAKTLSEDLAIAADRSQSSRAAQAAAKVQEAADAWM 151 ++ L D E R L+ +IE+L ++ EDL IAADR+QS RA + AA ++A W+ Sbjct: 86 ATVARARLAGDAEERRTLETRIEQLTQSFEEDLEIAADRAQSERATREAAAAKDAVTHWL 145 Query: 152 ALHRRAVGSSTEGSAAADPREPGATVGDVDRYSKIVSQQVELLVNYTAGDGFLFRQKALS 211 A RR G R+ +D ++ + Q ++LL+N+TAGDGF +RQ A + Sbjct: 146 A-ARREFGPD---------RQTVDVWQALDAHAAVAEQHIDLLINFTAGDGFAYRQTARA 195 Query: 212 TIKRDLQLNVAGLTVALFLSALFSWLLARRIIGPVAIASRAARSIAGGDLDATIPKGGTD 271 + RD++L+V +A+ LS + S LL R+I+ PVA AS A IA G+L T+P+GG D Sbjct: 196 VVARDVRLSVFATALAIILSGIVSLLLLRQIMRPVADASTVASRIAAGELSVTVPEGGAD 255 Query: 272 ELGTLLTAMETMRDNIRRMVNQEVSQRRSAQARLSDALETSREGVVLLDADGQVALANSR 331 E G LL AM MRDNI M+ EV+QRRSAQ+ L+DA++ S EG+V++D G++ LAN R Sbjct: 256 EFGALLQAMAVMRDNIAAMMQAEVAQRRSAQSLLADAVQGSIEGIVVVDPQGRIVLANER 315 Query: 332 ASELIRLSPQLLQSNPLDAAR--------LA-PADGDIDGFASEAQLSDGRWLRVSRSET 382 A+ L+ P PL AR LA P G + +EAQ +DGRWLR+SRS T Sbjct: 316 AAALLGFDPAETGHRPLSEARGSAVADTLLAMPGSGTL---TAEAQSADGRWLRISRSPT 372 Query: 383 QEGGVVLVYSDISALKQQKAELHETNLRLDAALTHMSQGLCLYNSEARLQVANRRLQVAN 442 +EGG V V SD+S LK+Q+ +L +N +LDAAL++M QGLCLY+ A L V N Sbjct: 373 REGGFVAVCSDVSLLKEQEDQLKRSNSQLDAALSNMLQGLCLYD-------AAGGLLVYN 425 Query: 443 RRFCEIFDISPELVIPGMTX-EVLDLSIAAGNHGERTVADLLAERERSMAQH-DGNYLQH 500 RRFC++ + + GM+ EV L N G + LL E++ + + G+ Sbjct: 426 RRFCDMLGLDAGELRTGMSLREVARLVETTSNDG--SAFGLLMEQQALLHRGVSGSLCCP 483 Query: 501 LSDGRIIAIAQRPTSDGGWLVTCEDVTEQQRAESQIAFMXKK 542 + I+++ Q+PT++GGW+ T EDVT++ AE++I M ++ Sbjct: 484 IRKDCIVSLKQQPTAEGGWIATYEDVTQRYEAEARIITMARR 525 >ref|YP_001640236.1| diguanylate cyclase [Methylobacterium extorquens PA1] gb|ABY31165.1| diguanylate cyclase [Methylobacterium extorquens PA1] Length = 954 Score = 339 bits (870), Expect = 8e-91, Method: Compositional matrix adjust. Identities = 222/537 (41%), Positives = 329/537 (61%), Gaps = 29/537 (5%) Query: 16 FRFAAGCLSRLALTKG--SIRSQILIFCLAMSAVAVALGGYSILGIRHAGDLVAKTFDES 73 F F+ G + A+ +G +IR +I + L +SA+ ALGGY+ GI G LV KT+D S Sbjct: 8 FGFSLGRTAVGAVLRGGRTIRGRIFLAFLMLSAITAALGGYAAFGIMTTGALVDKTYDRS 67 Query: 74 LMSINYARAAGADFATMRVASSQRLLTTDPEIRANLDGQIEKLAKTLSEDLAIAADRSQS 133 LM+INYARAA D A ++ A ++ L D R L+ +IE L ++ EDL IAADR+QS Sbjct: 68 LMAINYARAAATDLAMLQAAVARARLAGDSAERRTLEARIEALTQSFEEDLEIAADRAQS 127 Query: 134 SRAAQAAAKVQEAADAWMALHRRAVGSSTEGSAAADPREPGATVGD-VDRYSKIVSQQVE 192 RA + AA ++A W+A RR G P + A V +D ++ + Q ++ Sbjct: 128 ERATREAAAAKDAVAHWLA-ARREFG----------PDQQTADVWQALDAHAAVAEQHID 176 Query: 193 LLVNYTAGDGFLFRQKALSTIKRDLQLNVAGLTVALFLSALFSWLLARRIIGPVAIASRA 252 LL+N+TAGDGF +RQ A + + RD+ L++ ++A+ LS + + LL R+I+ PVA AS Sbjct: 177 LLINFTAGDGFSYRQTARADVARDVGLSIFATSLAIVLSGIVALLLLRQIVRPVADASTV 236 Query: 253 ARSIAGGDLDATIPKGGTDELGTLLTAMETMRDNIRRMVNQEVSQRRSAQARLSDALETS 312 A IA G+L +P+GG DE G LL AM MRDNI M+ +EV+QRRSAQ+ L+DA++ S Sbjct: 237 ASRIAAGELTVRVPEGGDDEFGALLRAMAVMRDNIAAMMQEEVAQRRSAQSLLADAVQGS 296 Query: 313 REGVVLLDADGQVALANSRASELIRLSPQLLQSNPLDAAR---LAPADGDIDGFAS---E 366 EG+V++DA G++ LAN+RA+ L+ + PL AR +A A + G A+ E Sbjct: 297 IEGIVVVDAAGRIVLANARAAALLGIDQAEPGHRPLSDARGSAVADALLAMPGHATLTAE 356 Query: 367 AQLSDGRWLRVSRSETQEGGVVLVYSDISALKQQKAELHETNLRLDAALTHMSQGLCLYN 426 +DGRWLR+SRS T+EGG V V SD+S LK+Q+ +L +N +LDAAL++M QGLCLY+ Sbjct: 357 THTADGRWLRISRSPTREGGFVAVCSDVSLLKEQEDQLKRSNAQLDAALSNMLQGLCLYD 416 Query: 427 SEARLQVANRRLQVANRRFCEIFDISPELVIPGMTXEVLDLSIAAGNHGERTVADLLAER 486 +E L V NRRFC++F + + PGM+ + + A + R + DLL E+ Sbjct: 417 AEG-------GLLVYNRRFCDMFGVDAAALRPGMSIRDVARLVEASSDNGRAI-DLLVEQ 468 Query: 487 ERSMAQH-DGNYLQHLSDGRIIAIAQRPTSDGGWLVTCEDVTEQQRAESQIAFMXKK 542 E + + + + I+A+ Q+PT++GGW+ T EDVT++ AE++I M +K Sbjct: 469 EALLQRGLSASLCCPIRTDCIVALKQQPTAEGGWIATYEDVTQRYEAEARIITMARK 525 >ref|YP_003069034.1| diguanylate cyclase/phosphodiesterase [Methylobacterium extorquens DM4] emb|CAX25183.1| putative diguanylate cyclase (GGDEF) /phosphodiesterase (EAL), with PAS sensor, signal transduction histidine kinase and methyl-accepting chemotaxis domains; putative membrane-associated protein [Methylobacterium extorquens DM4] Length = 954 Score = 338 bits (868), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 220/536 (41%), Positives = 326/536 (60%), Gaps = 27/536 (5%) Query: 16 FRFAAGCLSRLALTKG--SIRSQILIFCLAMSAVAVALGGYSILGIRHAGDLVAKTFDES 73 F F+ G + A+ +G +IR +I + L +SA+ ALGGY+ GI G LV KT+D S Sbjct: 8 FGFSLGRTAVGAVLRGGRTIRGRIFLAFLMLSAITAALGGYAAFGIMTTGALVDKTYDRS 67 Query: 74 LMSINYARAAGADFATMRVASSQRLLTTDPEIRANLDGQIEKLAKTLSEDLAIAADRSQS 133 LM+INYARAA D A ++ A ++ L D R L+ +IE L ++ EDL IAADR+QS Sbjct: 68 LMAINYARAAATDLAMLQAAVARARLAGDSAERRTLEARIEALTQSFEEDLEIAADRAQS 127 Query: 134 SRAAQAAAKVQEAADAWMALHRRAVGSSTEGSAAADPREPGATVGDVDRYSKIVSQQVEL 193 RA + AA ++A W+A RR G + S +D ++ + Q ++L Sbjct: 128 ERATREAAAAKDAVAHWLA-ARREFGPDQQTSDVWQA---------LDAHAAVAEQHIDL 177 Query: 194 LVNYTAGDGFLFRQKALSTIKRDLQLNVAGLTVALFLSALFSWLLARRIIGPVAIASRAA 253 L+N+TAGDGF +RQ A + + RD+ L++ ++A+ LS + + LL R+I+ PVA AS A Sbjct: 178 LINFTAGDGFSYRQTARADVTRDVGLSIFATSLAIVLSGIVALLLLRQIVRPVADASTVA 237 Query: 254 RSIAGGDLDATIPKGGTDELGTLLTAMETMRDNIRRMVNQEVSQRRSAQARLSDALETSR 313 IA G+L +P+GG DE G LL AM MRDNI M+ +EV+QRRSAQ L+DA++ S Sbjct: 238 SRIAAGELTVRVPEGGDDEFGALLRAMAVMRDNIAAMMQEEVAQRRSAQGLLADAVQGSI 297 Query: 314 EGVVLLDADGQVALANSRASELIRLSPQLLQSNPLDAAR---LAPADGDIDGFAS---EA 367 EG+V++DA G++ LAN+RA+ L+ + PL AR +A A + G A+ E Sbjct: 298 EGIVVVDAAGRIVLANARAAALLGIDQAEPGHRPLSDARGSAVADALLAMPGHATLTAET 357 Query: 368 QLSDGRWLRVSRSETQEGGVVLVYSDISALKQQKAELHETNLRLDAALTHMSQGLCLYNS 427 +DGRWLR+SRS T+EGG V V SD+S LK+Q+ +L +N +LDAAL++M QGLCLY++ Sbjct: 358 HTADGRWLRISRSPTREGGFVAVCSDVSLLKEQEDQLKRSNAQLDAALSNMLQGLCLYDA 417 Query: 428 EARLQVANRRLQVANRRFCEIFDISPELVIPGMTXEVLDLSIAAGNHGERTVADLLAERE 487 E L V NRRFC++F + + PGM+ + + A + R + DLL E+E Sbjct: 418 EG-------GLLVYNRRFCDMFGVDAAALRPGMSIRDVARLVEASSDNGRAI-DLLVEQE 469 Query: 488 RSMAQH-DGNYLQHLSDGRIIAIAQRPTSDGGWLVTCEDVTEQQRAESQIAFMXKK 542 + + + + I+A+ Q+PT++GGW+ T EDVT++ AE++I M +K Sbjct: 470 ALLQRGLSASLCCPIRTDCIVALKQQPTAEGGWIATYEDVTQRYEAEARIITMARK 525 >ref|YP_002964000.1| diguanylate cyclase (GGDEF)/phosphodiesterase (EAL), with PAS sensor, signal transduction histidine kinase and methyl-accepting chemotaxis domains; membrane-associated protein [methylobacterium extorquens AM1] gb|ACS40723.1| putative diguanylate cyclase (GGDEF)/phosphodiesterase (EAL), with PAS sensor, signal transduction histidine kinase and methyl-accepting chemotaxis domains; putative membrane-associated protein [Methylobacterium extorquens AM1] Length = 954 Score = 338 bits (866), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 222/540 (41%), Positives = 327/540 (60%), Gaps = 35/540 (6%) Query: 16 FRFAAGCLSRLALTKG--SIRSQILIFCLAMSAVAVALGGYSILGIRHAGDLVAKTFDES 73 F F+ G + A+ +G +IR +I + L +SA+ ALGGY+ GI G LV KT+D S Sbjct: 8 FGFSLGRTAVGAVLRGGRTIRGRIFLAFLMLSAITAALGGYAAFGIMTTGALVDKTYDRS 67 Query: 74 LMSINYARAAGADFATMRVASSQRLLTTDPEIRANLDGQIEKLAKTLSEDLAIAADRSQS 133 LM+INYARAA D A ++ A ++ L D R L+ +IE L ++ EDL IAADR+QS Sbjct: 68 LMAINYARAAATDLAMLQAAVARARLAGDSAERRTLEARIEALTQSFEEDLEIAADRAQS 127 Query: 134 SRAAQAAAKVQEAADAWMALHRRAVGSSTEGSAAADPREPGATVGDV----DRYSKIVSQ 189 RA + AA ++A W+A RR G P DV D ++ + Q Sbjct: 128 ERATREAAAAKDAVAHWLA-ARREFG-------------PDQQTVDVWQALDAHAAVAEQ 173 Query: 190 QVELLVNYTAGDGFLFRQKALSTIKRDLQLNVAGLTVALFLSALFSWLLARRIIGPVAIA 249 ++LL+N+TAGDGF +RQ A + + RD++L++ ++A+ LS + + LL R+I+ PVA A Sbjct: 174 HIDLLINFTAGDGFSYRQTARADVARDVRLSIFATSLAIVLSGIVALLLLRQIVRPVADA 233 Query: 250 SRAARSIAGGDLDATIPKGGTDELGTLLTAMETMRDNIRRMVNQEVSQRRSAQARLSDAL 309 S A IA G+L +P+GG DE G LL AM MRDNI M+ +EV+QRRSAQ+ L+DA+ Sbjct: 234 STVASRIAAGELTVRVPEGGDDEFGALLRAMAVMRDNIAAMMQEEVAQRRSAQSLLADAV 293 Query: 310 ETSREGVVLLDADGQVALANSRASELIRLSPQLLQSNPLDAAR---LAPADGDIDGFAS- 365 + S EG+V++DA G + LAN+RA+ L+ + PL AR +A A + G A+ Sbjct: 294 QGSIEGIVVVDAGGCIVLANARAAALLGIDQAEPGHRPLSDARGSAVADALLAMPGHATL 353 Query: 366 --EAQLSDGRWLRVSRSETQEGGVVLVYSDISALKQQKAELHETNLRLDAALTHMSQGLC 423 E +DGRWLR+SRS T+EGG V V SD+S LK+Q+ +L +N +LDAAL++M QGLC Sbjct: 354 TAETHTADGRWLRISRSPTREGGFVAVCSDVSLLKEQEDQLKRSNAQLDAALSNMLQGLC 413 Query: 424 LYNSEARLQVANRRLQVANRRFCEIFDISPELVIPGMTXEVLDLSIAAGNHGERTVADLL 483 LY++E L V NRRFC++F + + PGM+ + + A + R + DLL Sbjct: 414 LYDAEG-------GLLVYNRRFCDMFGVDAAALRPGMSIRDVARLVEASSDNGRAI-DLL 465 Query: 484 AERERSMAQH-DGNYLQHLSDGRIIAIAQRPTSDGGWLVTCEDVTEQQRAESQIAFMXKK 542 E+E + + + + I+A+ Q+PT++GGW+ T EDVT++ AE++I M +K Sbjct: 466 VEQEALLQRGLSASLCCPIRTDCIVALKQQPTAEGGWIATYEDVTQRYEAEARIITMARK 525