BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0177.0005.p (330 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_07027317.1| diguanylate cyclase/phosphodiesterase with PA... 471 e-131 ref|NP_768191.1| hypothetical protein blr1551 [Bradyrhizobium ja... 428 e-118 ref|YP_001755360.1| PAS/PAC sensor-containing diguanylate cyclas... 427 e-117 ref|YP_002419756.1| diguanylate cyclase/phosphodiesterase [Methy... 411 e-113 ref|YP_003066729.1| diguanylate cyclase/phosphodiesterase [Methy... 411 e-113 >ref|ZP_07027317.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Afipia sp. 1NLS2] gb|EFI51073.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Afipia sp. 1NLS2] Length = 958 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 215/320 (67%), Positives = 261/320 (81%) Query: 1 VGAPYELNGHRVVVGCSVGISMSPGDGTTGEKLLKNADVALYRAKMEGRGTWRFFEPAMD 60 +G PYE +G R+V+GCSVGIS+SPGDGT+GEKLLKNADVALYRAK EGRG WRFFE MD Sbjct: 628 LGEPYEFDGQRIVIGCSVGISLSPGDGTSGEKLLKNADVALYRAKAEGRGIWRFFETEMD 687 Query: 61 ASLQRRRALELDLREAMAKDEFSLYYQPLYDLHLDRICGFEALLRWHHPKRGMVSPDQFI 120 SLQ+RRALELDLREAM K +F L+YQPLYD+ LD++CGFEALLRW HP G+V+P+QFI Sbjct: 688 ESLQKRRALELDLREAMDKKQFELFYQPLYDIDLDKVCGFEALLRWRHPVHGVVTPEQFI 747 Query: 121 PIAEEIGLIGPLGEWVLNRACEQATTWPGEMKLAVNVSAVQFRDADFIDVVVNALAASKL 180 +AEEIGLI PLGEWV+ +AC +A WP ++K+AVNVS+VQFR ++ +ALA+SKL Sbjct: 748 SVAEEIGLIIPLGEWVIQQACREAALWPDDLKVAVNVSSVQFRSPRLLEAFSSALASSKL 807 Query: 181 SPRRLELEITESVFLANSNETLATLHKLKAQGLRIALDDFGTGYSSLSYLRSFPFDKLKI 240 P+RLELEITESV L N+ ET TLHKL+A GLRIALDDFGTGYSSLSYLRSFPFDKLKI Sbjct: 808 PPKRLELEITESVLLTNNGETTETLHKLRALGLRIALDDFGTGYSSLSYLRSFPFDKLKI 867 Query: 241 DKSFVRDATATHGSKSIVRAVISLGRSLGMTTIAEGIETVEQLDHMRAEGCNEAQGFLLS 300 D+SFVRD T GS+ I+RA++SLG+SLGM T AEG++ + QL+++ AEGCNE QG+ S Sbjct: 868 DQSFVRDLTDAEGSRVIIRAIVSLGKSLGMRTTAEGVQNIAQLNYIAAEGCNEVQGYFFS 927 Query: 301 HPVPVTEIAAKILELRNGFK 320 PV +E+ + I RN K Sbjct: 928 KPVAASEVPSVIRTCRNRLK 947 >ref|NP_768191.1| hypothetical protein blr1551 [Bradyrhizobium japonicum USDA 110] dbj|BAC46816.1| blr1551 [Bradyrhizobium japonicum USDA 110] Length = 566 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 204/259 (78%), Positives = 224/259 (86%) Query: 61 ASLQRRRALELDLREAMAKDEFSLYYQPLYDLHLDRICGFEALLRWHHPKRGMVSPDQFI 120 ASLQ RRALELDLREAM KDEF+L+YQP+YDL +DRI GFEALLRW HPKRG+V PDQFI Sbjct: 297 ASLQSRRALELDLREAMDKDEFTLFYQPIYDLRMDRISGFEALLRWPHPKRGLVPPDQFI 356 Query: 121 PIAEEIGLIGPLGEWVLNRACEQATTWPGEMKLAVNVSAVQFRDADFIDVVVNALAASKL 180 PIAE+IGLI PLGEWVLNRACEQA WP E+K+AVNVSA Q RD F +V+ NAL AS L Sbjct: 357 PIAEDIGLITPLGEWVLNRACEQAAGWPSELKIAVNVSATQIRDPRFAEVIANALDASTL 416 Query: 181 SPRRLELEITESVFLANSNETLATLHKLKAQGLRIALDDFGTGYSSLSYLRSFPFDKLKI 240 +P RLELEITESV L NS ET+ATLHKLKAQGL IALDDFGTGYSSLSYLRSFPFDKLKI Sbjct: 417 APHRLELEITESVLLGNSAETVATLHKLKAQGLSIALDDFGTGYSSLSYLRSFPFDKLKI 476 Query: 241 DKSFVRDATATHGSKSIVRAVISLGRSLGMTTIAEGIETVEQLDHMRAEGCNEAQGFLLS 300 D+SFVRDATAT+GSK IVRA+ SLGRSLG+T AEG+ET+EQLD +RAEGCNEAQGFL S Sbjct: 477 DQSFVRDATATNGSKLIVRAITSLGRSLGITITAEGVETIEQLDQIRAEGCNEAQGFLFS 536 Query: 301 HPVPVTEIAAKILELRNGF 319 PVP TEIA+ IL LR G Sbjct: 537 RPVPATEIASTILNLRGGL 555 >ref|YP_001755360.1| PAS/PAC sensor-containing diguanylate cyclase/phosphodiesterase [Methylobacterium radiotolerans JCM 2831] gb|ACB24677.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) [Methylobacterium radiotolerans JCM 2831] Length = 960 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 201/312 (64%), Positives = 242/312 (77%) Query: 1 VGAPYELNGHRVVVGCSVGISMSPGDGTTGEKLLKNADVALYRAKMEGRGTWRFFEPAMD 60 VGAPY L H + VG ++GI+++P DG+ +KLLKNA+VAL R K E RG +RFFEPAMD Sbjct: 638 VGAPYSLTHHTISVGLTIGITLAPSDGSNADKLLKNAEVALDRGKTEARGAFRFFEPAMD 697 Query: 61 ASLQRRRALELDLREAMAKDEFSLYYQPLYDLHLDRICGFEALLRWHHPKRGMVSPDQFI 120 A L RR +E DLR+A+A++ F +YYQP+Y L DRICGFEALLRW+HP RG VSP +FI Sbjct: 698 ARLIARRIMERDLRDALAREAFEVYYQPIYSLETDRICGFEALLRWNHPVRGFVSPAEFI 757 Query: 121 PIAEEIGLIGPLGEWVLNRACEQATTWPGEMKLAVNVSAVQFRDADFIDVVVNALAASKL 180 PIAEE+GLI PLGEWVL RACE+A WP E+K+AVNVSAVQF A + V AL + L Sbjct: 758 PIAEELGLIVPLGEWVLRRACEEAARWPDELKVAVNVSAVQFTSASLVTAVREALRRTGL 817 Query: 181 SPRRLELEITESVFLANSNETLATLHKLKAQGLRIALDDFGTGYSSLSYLRSFPFDKLKI 240 RRLELEITE+V +AN T A LH L+A G+R+A+DDFGTGYSSLSYLRSFPFDK+KI Sbjct: 818 PGRRLELEITETVLVANPGATTAILHSLRALGVRVAMDDFGTGYSSLSYLRSFPFDKIKI 877 Query: 241 DKSFVRDATATHGSKSIVRAVISLGRSLGMTTIAEGIETVEQLDHMRAEGCNEAQGFLLS 300 D+SFVRD G+ IVRAVI LG SLGMTT AEG+ET QL H+RAEGC+E QG+L S Sbjct: 878 DQSFVRDLCVKDGTDFIVRAVIGLGASLGMTTTAEGVETEAQLAHLRAEGCDEVQGYLFS 937 Query: 301 HPVPVTEIAAKI 312 PVPV+E+A+ I Sbjct: 938 RPVPVSEVASVI 949 >ref|YP_002419756.1| diguanylate cyclase/phosphodiesterase [Methylobacterium chloromethanicum CM4] gb|ACK81828.1| diguanylate cyclase/phosphodiesterase [Methylobacterium chloromethanicum CM4] Length = 714 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/320 (63%), Positives = 241/320 (75%), Gaps = 1/320 (0%) Query: 1 VGAPYELNGHRVVVGCSVGISMSPGDGTTGEKLLKNADVALYRAKMEGRGTWRFFEPAMD 60 V APY L H V+VG S+GI+++PGDG E+L++ ADVALYRAK +GRG +RFFE MD Sbjct: 392 VSAPYALTHHSVIVGVSIGIALAPGDGLEPERLMQCADVALYRAKDDGRGVFRFFEAQMD 451 Query: 61 ASLQRRRALELDLREAMAKDEFSLYYQPLYDLHLDRICGFEALLRWHHPKRGMVSPDQFI 120 A LQ RRALELDLR A + F L+YQP+YDL +RI GFEALLRW HP RG VSP +F+ Sbjct: 452 ARLQARRALELDLRAAFEAEAFDLHYQPIYDLAEERISGFEALLRWTHPTRGRVSPAEFV 511 Query: 121 PIAEEIGLIGPLGEWVLNRACEQATTWPGEMKLAVNVSAVQFRDADFIDVVVNALAASKL 180 P+AEEIGLI PLGEWVL RAC +A+ WP +K+AVNVSA QF I V ALA S L Sbjct: 512 PLAEEIGLIVPLGEWVLRRACREASNWPDGLKVAVNVSAAQFTSIALIGTVQAALAESGL 571 Query: 181 SPRRLELEITESVFLANSNETLATLHKLKAQGLRIALDDFGTGYSSLSYLRSFPFDKLKI 240 S RRLELEITESV L N + T+A LH L+ G RIA+DDFGTGYSSLSYLRSFPFDK+K+ Sbjct: 572 SARRLELEITESVLLVNGSATVAILHGLRDLGARIAMDDFGTGYSSLSYLRSFPFDKMKV 631 Query: 241 DKSFVRDATATHGSKSIVRAVISLGRSLGMTTIAEGIETVEQLDHMRAEGCNEAQGFLLS 300 D+SF RD T GS IVRAV+SL SLGMTT AEG+ET EQL +R EGC+E QG+L S Sbjct: 632 DQSFTRDLTVEQGSGFIVRAVVSLASSLGMTTTAEGVETPEQLAWLREEGCDEVQGYLFS 691 Query: 301 HPVPVTEIAAKILELRNGFK 320 PVP +E+ A +L++ NG K Sbjct: 692 PPVPASELPA-LLDIWNGRK 710 >ref|YP_003066729.1| diguanylate cyclase/phosphodiesterase [Methylobacterium extorquens DM4] emb|CAX22711.1| putative diguanylate cyclase (GGDEF) /phosphodiesterase (EAL) with PAS sensor; putative membrane-associated protein precursor [Methylobacterium extorquens DM4] Length = 714 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 204/320 (63%), Positives = 241/320 (75%), Gaps = 1/320 (0%) Query: 1 VGAPYELNGHRVVVGCSVGISMSPGDGTTGEKLLKNADVALYRAKMEGRGTWRFFEPAMD 60 V APY L H V+VG S+GI+++PGDG E+L++ ADVALYRAK +GRG +RFFE MD Sbjct: 392 VSAPYALAHHSVIVGVSIGIALAPGDGLEPERLMQCADVALYRAKDDGRGVFRFFEAQMD 451 Query: 61 ASLQRRRALELDLREAMAKDEFSLYYQPLYDLHLDRICGFEALLRWHHPKRGMVSPDQFI 120 A LQ RRALELDLR A + F L+YQP+YDL +RI GFEALLRW HP RG VSP +F+ Sbjct: 452 ARLQARRALELDLRAAFEAEAFDLHYQPIYDLAEERISGFEALLRWTHPTRGRVSPAEFV 511 Query: 121 PIAEEIGLIGPLGEWVLNRACEQATTWPGEMKLAVNVSAVQFRDADFIDVVVNALAASKL 180 P+AEEIGLI PLGEWVL RAC +A+ WP +K+AVNVSA QF I V ALA S L Sbjct: 512 PLAEEIGLIVPLGEWVLRRACREASGWPDGLKVAVNVSAAQFTSIALIGTVQAALAESGL 571 Query: 181 SPRRLELEITESVFLANSNETLATLHKLKAQGLRIALDDFGTGYSSLSYLRSFPFDKLKI 240 S RRLELEITESV L N + T+A LH L+ G RIA+DDFGTGYSSLSYLRSFPFDK+K+ Sbjct: 572 SARRLELEITESVLLVNGSATVAILHGLRDLGARIAMDDFGTGYSSLSYLRSFPFDKMKV 631 Query: 241 DKSFVRDATATHGSKSIVRAVISLGRSLGMTTIAEGIETVEQLDHMRAEGCNEAQGFLLS 300 D+SF RD T GS IVRAV+SL SLGMTT AEG+ET EQL +R EGC+E QG+L S Sbjct: 632 DQSFTRDLTVEQGSGFIVRAVVSLASSLGMTTTAEGVETPEQLAWLREEGCDEVQGYLFS 691 Query: 301 HPVPVTEIAAKILELRNGFK 320 PVP +E+ A +LE+ NG K Sbjct: 692 PPVPASELPA-LLEIWNGRK 710