BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0033.p (347 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_773703.1| 2-ketogluconate reductase [Bradyrhizobium japon... 649 0.0 ref|YP_004545941.1| D-isomer specific 2-hydroxyacid dehydrogenas... 264 2e-68 ref|YP_001665933.1| NAD-binding D-isomer specific 2-hydroxyacid ... 251 9e-65 ref|YP_004820408.1| NAD-binding D-isomer specific 2-hydroxyacid ... 248 8e-64 ref|ZP_03147731.1| D-isomer specific 2-hydroxyacid dehydrogenase... 236 6e-60 >ref|NP_773703.1| 2-ketogluconate reductase [Bradyrhizobium japonicum USDA 110] dbj|BAC52328.1| blr7063 [Bradyrhizobium japonicum USDA 110] Length = 387 Score = 649 bits (1675), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/347 (92%), Positives = 337/347 (97%) Query: 1 MSKFRVVTPKGASFTVAGGGYDYEREALDPIGAEIVEAPANEAEFIAAARTADAIYAKGM 60 M K+RVVTPKGASFTVAG Y YEREALDPI AEI+EAPANEAEFIAAA+ ADAIYAKG+ Sbjct: 41 MPKYRVVTPKGASFTVAGSDYSYEREALDPIDAEIIEAPANEAEFIAAAKNADAIYAKGI 100 Query: 61 PITKTVIDALENCKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLAG 120 PITK++IDALE+CKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLAG Sbjct: 101 PITKSIIDALESCKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLAG 160 Query: 121 FRHLIEQDKMVRTGRWAEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRMVAY 180 FR L+EQD+MVR+GRWAEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRM+AY Sbjct: 161 FRRLVEQDRMVRSGRWAEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRMMAY 220 Query: 181 DPFIQETLMYDHGVIPSTLSEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKSAIFINT 240 DPFIQETLMYDHGVIP+TL+EVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKK +IFINT Sbjct: 221 DPFIQETLMYDHGVIPATLNEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKGSIFINT 280 Query: 241 GRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNPMLGMDNVTLTAHVASASARFDE 300 GRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNP+L M+NVTLTAHVASASARFDE Sbjct: 281 GRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNPILSMENVTLTAHVASASARFDE 340 Query: 301 ARKRRVGYELSLVLQGMWPVSCVNPSVLQDTALRRWQPVSMDRGPNS 347 ARKRRVGYELSLVLQGMWPVSCVNPSVLQ TALRRWQPVSMDRGPNS Sbjct: 341 ARKRRVGYELSLVLQGMWPVSCVNPSVLQSTALRRWQPVSMDRGPNS 387 >ref|YP_004545941.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Desulfotomaculum ruminis DSM 2154] gb|AEG60655.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Desulfotomaculum ruminis DSM 2154] Length = 331 Score = 264 bits (675), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 143/328 (43%), Positives = 194/328 (59%), Gaps = 8/328 (2%) Query: 1 MSKFRVVTPKGASFTVAGGGYDYEREALDPIGAEIVEAPAN-EAEFIAAARTADAIYAKG 59 MS +VV T+ YE E L IG +V A E E I A + AD + + Sbjct: 1 MSNLKVVVTDYEYSTL-----KYEEEVLAQIGVSLVPAQCKTEEELIEACKDADGLLNQY 55 Query: 60 MPITKTVIDALENCKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLA 119 +T VI AL+ CKVI VG+++VD+ AAT +GI V N+PD ++EV+DHAM LLLA Sbjct: 56 AQLTPRVIQALDRCKVIGRYGVGINTVDLAAATEKGICVVNVPDYCMDEVSDHAMALLLA 115 Query: 120 GFRHLIEQDKMVRTGRWAEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRMVA 179 R + + V+ G W + + RL G+ LG ISFGR+A+ +AK+A FGL ++ Sbjct: 116 CVRKTVLMNNEVKKGNW--DYKVSVPVYRLRGKNLGLISFGRIAQTLAKKAQAFGLNLLV 173 Query: 180 YDPFIQETLMYDHGVIPSTLSEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKSAIFIN 239 +DP+I + + GV +L E+L SDF+S+HAP E H+L EK F+ MKKSA IN Sbjct: 174 FDPYIPDAVAEQFGVKKVSLEELLQTSDFISVHAPLNAETEHLLGEKEFKMMKKSAFIIN 233 Query: 240 TGRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNPMLGMDNVTLTAHVASASARFD 299 TGRG +DEE+L+KALQEGWIA A LDVLE EP +P+L MDNV L HVA S Sbjct: 234 TGRGPVIDEEALVKALQEGWIAGAGLDVLEIEPVRSESPLLKMDNVILNPHVAWYSEEAG 293 Query: 300 EARKRRVGYELSLVLQGMWPVSCVNPSV 327 + + ++ VLQG +P + VN + Sbjct: 294 TDLQIKAAQGVAEVLQGYYPRNLVNKEI 321 >ref|YP_001665933.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Thermoanaerobacter pseudethanolicus ATCC 33223] ref|YP_004186918.1| D-isomer specific 2-hydroxyacid dehydrogenase protein [Thermoanaerobacter brockii subsp. finnii Ako-1] gb|ABY95597.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Thermoanaerobacter pseudethanolicus ATCC 33223] gb|ADV80535.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Thermoanaerobacter brockii subsp. finnii Ako-1] Length = 332 Score = 251 bits (642), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 140/337 (41%), Positives = 197/337 (58%), Gaps = 12/337 (3%) Query: 1 MSKFRVVTPKGASFTVAGGGYDYEREALDPIGAEIVEAPAN-EAEFIAAARTADAIYAKG 59 MS+ +VV F A YE E L G +V A E E I A + AD + + Sbjct: 1 MSQLKVVV---TDFEYAN--LKYEEEVLSKAGITLVPAQCKTEEELILACKDADGLINQY 55 Query: 60 MPITKTVIDALENCKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLA 119 IT+ VI+AL+ CKVI +GVD++D+KAATARGI V N+PD ++EV+DHA+ LLL Sbjct: 56 AKITRRVIEALDKCKVIARYGIGVDTIDIKAATARGICVVNVPDYCMDEVSDHALALLLG 115 Query: 120 GFRHLIEQDKMVRTGRW--AEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRM 177 R ++ + V+ G W +P I RL G+ LG + FGR+ R +A++A PFG + Sbjct: 116 CARKVVLMNNAVKAGTWDFKISKP----IYRLRGKVLGLVGFGRIPRTLAEKARPFGFDI 171 Query: 178 VAYDPFIQETLMYDHGVIPSTLSEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKSAIF 237 + YDP+I + + +G L E++S+SDFVS+HAP + +H++ E MK SA Sbjct: 172 LVYDPYITQEDVEPYGATLVGLEELMSKSDFVSVHAPLTEKTYHLIGETEIGLMKPSAFI 231 Query: 238 INTGRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNPMLGMDNVTLTAHVASASAR 297 INT RG +DE++LIKALQE IA A LDVLE+EP +NP+L MDNV +T HVA S Sbjct: 232 INTARGPVIDEKALIKALQEKRIAGAGLDVLEQEPTPSDNPLLKMDNVIITPHVAWYSEE 291 Query: 298 FDEARKRRVGYELSLVLQGMWPVSCVNPSVLQDTALR 334 + + + ++ VL G WP VN V + L+ Sbjct: 292 SEAELRTKAAQGVADVLLGYWPKYLVNKEVKEKLQLK 328 >ref|YP_004820408.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Thermoanaerobacter wiegelii Rt8.B1] gb|AEM79124.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Thermoanaerobacter wiegelii Rt8.B1] Length = 331 Score = 248 bits (634), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 133/319 (41%), Positives = 190/319 (59%), Gaps = 7/319 (2%) Query: 19 GGYDYEREALDPIGAEIVEAPA-NEAEFIAAARTADAIYAKGMPITKTVIDALENCKVIT 77 G YE L G ++ A +E E I+ + AD + + IT+ VI+AL+NCKVI Sbjct: 14 GDLRYEERVLKEAGITLIPAQCKSEEELISVCKDADGLINQYAKITRRVIEALDNCKVIA 73 Query: 78 LGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLAGFRHLIEQDKMVRTGRW- 136 VGVD++D+KAATARGI V N+PD ++EV+DHA+ LLL R ++ + V+ G W Sbjct: 74 RYGVGVDTIDIKAATARGICVVNVPDYCMDEVSDHALALLLGCARKVVLMNNAVKAGTWD 133 Query: 137 -AEGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRMVAYDPFIQETLMYDHGVI 195 +P I RL G+ LG + FGR+ R +A++A PFG ++ YDP+I + + +G Sbjct: 134 FKISKP----IYRLRGKVLGLVGFGRIPRTLAEKARPFGFDILVYDPYITQADVEPYGAT 189 Query: 196 PSTLSEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKSAIFINTGRGATVDEESLIKAL 255 L E++++SDFVS+HAP + +H++ E MK SA INT RG +DE++LIKAL Sbjct: 190 LVGLEELMAKSDFVSVHAPLTEKTYHLIGETEIGLMKPSAFIINTARGPVIDEKALIKAL 249 Query: 256 QEGWIAHAALDVLEKEPPSHNNPMLGMDNVTLTAHVASASARFDEARKRRVGYELSLVLQ 315 QE IA A LDVLE+EP +NP+L MDNV +T HVA S + + + ++ VL Sbjct: 250 QEKRIAGAGLDVLEQEPTPSDNPLLKMDNVIITPHVAWYSEEAEAELRTKAAQGVADVLL 309 Query: 316 GMWPVSCVNPSVLQDTALR 334 G WP VN V L+ Sbjct: 310 GYWPKYLVNKEVKDKIMLK 328 >ref|ZP_03147731.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Geobacillus sp. G11MC16] gb|EDY06021.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Geobacillus sp. G11MC16] Length = 334 Score = 236 bits (601), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 136/337 (40%), Positives = 199/337 (59%), Gaps = 12/337 (3%) Query: 1 MSKFRVVTPKGASFTVAGGGYDYEREALDPIGAEIVEAPA-NEAEFIAAARTADAIYAKG 59 M K++VV T+ E+E L P+ + V A E E IA + ADAI + Sbjct: 1 MKKYKVVVTDYEFQTLKP-----EQEVLSPLNVDFVAAQCYTEEEVIAVCKDADAIINQY 55 Query: 60 MPITKTVIDALENCKVITLGSVGVDSVDVKAATARGIPVTNIPDTFIEEVADHAMMLLLA 119 PI+ VID LE CKVI+ VGV++VDV AAT +GI V N+ D ++EV+DHA+ LLL+ Sbjct: 56 APISAKVIDQLEKCKVISRYGVGVNTVDVDAATEKGIIVANVTDYSVDEVSDHALALLLS 115 Query: 120 GFRHLIEQDKMVRTGRWA--EGRPALLKIPRLMGQTLGFISFGRVARAVAKRAAPFGLRM 177 R +++ + V++G W G+P I RL G+TLG + FGR+ +A+AK+A GL + Sbjct: 116 LARKIVKLNDEVKSGVWNFNVGKP----IYRLRGRTLGLVGFGRIPQALAKKAQSLGLYV 171 Query: 178 VAYDPFIQETLMYDHGVIPSTLSEVLSQSDFVSMHAPARPEVHHMLTEKHFRQMKKSAIF 237 +AYDP+I + V L+++ QSD++S+HAP E M++++ F KK I Sbjct: 172 IAYDPYIPAKVARQFNVHLVELNDLFRQSDYISVHAPLTKETKGMISDEQFHLAKKELII 231 Query: 238 INTGRGATVDEESLIKALQEGWIAHAALDVLEKEPPSHNNPMLGMDNVTLTAHVASASAR 297 +NT RG +DE SLI+ALQ+G I+ A LDV E EP +NP+L M+NV +T H+A S Sbjct: 232 VNTARGPVIDESSLIRALQDGKISGAGLDVTEYEPIHPDNPLLQMENVVITPHIAWYSEE 291 Query: 298 FDEARKRRVGYELSLVLQGMWPVSCVNPSVLQDTALR 334 + KR+ ++ VL G +P VNP V + L+ Sbjct: 292 SEMELKRKTAQNVADVLSGCYPRYLVNPRVKEKVRLK 328