BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0004.p (438 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774809.1| hypothetical protein blr8169 [Bradyrhizobium ja... 679 0.0 ref|YP_001243149.1| mechanosensitive ion channel protein [Bradyr... 637 e-180 ref|YP_530473.1| mechanosensitive ion channel MscS [Rhodopseudom... 619 e-175 ref|YP_483680.1| MscS mechanosensitive ion channel [Rhodopseudom... 610 e-172 ref|YP_001202887.1| mechanosensitive ion channel protein [Bradyr... 601 e-170 >ref|NP_774809.1| hypothetical protein blr8169 [Bradyrhizobium japonicum USDA 110] dbj|BAC53434.1| blr8169 [Bradyrhizobium japonicum USDA 110] Length = 456 Score = 679 bits (1752), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/438 (79%), Positives = 392/438 (89%) Query: 1 MDMSLKDVLESIQMAARSVGAEVASPWFYLQFGIILAAAGLAYAADTAIHARVDMSSLAM 60 MDM LKD +E +Q ARSVGAE++SPWFYLQFG+ILAAAG+AYAA+ + RVDM+SLAM Sbjct: 19 MDMDLKDFMEFVQTTARSVGAEISSPWFYLQFGLILAAAGIAYAAEAGVRNRVDMTSLAM 78 Query: 61 RWPLPLRHFARVMVKSASTAVFAVLMIISRIVMWHATWPSRSYLIAVSAKLALAWLVIRL 120 WPLPLRHF+RVMV SASTAVF +L+IISR VM+HATWPSRSYL+ V+AK+ LAWL IRL Sbjct: 79 GWPLPLRHFSRVMVSSASTAVFTLLVIISRFVMYHATWPSRSYLLMVAAKMGLAWLAIRL 138 Query: 121 VTSVIDNAFIVKLVSIAAWVVAALSIIGQLDWAAETLDSFAVVVGGLRLTPLLLIKAGAL 180 VTSV+ NAFIVKLVSI AW VAALSI+GQLD + LDSFA+V+GGLRLTPLL+IKAGAL Sbjct: 139 VTSVLRNAFIVKLVSITAWFVAALSILGQLDATIDLLDSFAIVLGGLRLTPLLVIKAGAL 198 Query: 181 LIVALWLSNIASNFIDGQITRSTDLTPSIQVLLVKIIRIGLMVVAVAIALSAVGINLSAL 240 L++ALW +NIASNF + +I +TDLTPS+QVLLVKIIRIGL+ +A+ IAL AVGI+LSAL Sbjct: 199 LLIALWATNIASNFAESRINSTTDLTPSVQVLLVKIIRIGLLAIAIVIALGAVGIDLSAL 258 Query: 241 AVLSGAVGVGIGFGLQKIVANFISGIILLVDKSVKPGDLVTIGDSQGRISAMKTRYISVA 300 AV SGAVGVGIG GLQKIVANFISGIILL DKSVKPGDLVTIGD+ GRISAMKTRYISVA Sbjct: 259 AVFSGAVGVGIGIGLQKIVANFISGIILLADKSVKPGDLVTIGDNTGRISAMKTRYISVA 318 Query: 301 AGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKIAFAVNYDADPRLVCKLAIDTAAAHARA 360 AGDGREFL+PNEDLVTQKV NWTYTDKNTLVKIAF NYDADPRLVCKLAI+TA AH RA Sbjct: 319 AGDGREFLVPNEDLVTQKVVNWTYTDKNTLVKIAFGTNYDADPRLVCKLAIETAIAHPRA 378 Query: 361 LKGKPPNCILTEFAELGMKFSLTFWIADPDGMDNVKSDVMLALWDAFKQQGIRVPYPVRE 420 K KPPNCILTEFAE GMKFSLT W+ADPDGMDNVKSDVMLALW+AFK++GIRVPYPVRE Sbjct: 379 QKAKPPNCILTEFAEAGMKFSLTLWLADPDGMDNVKSDVMLALWEAFKREGIRVPYPVRE 438 Query: 421 LRIRGGALPVESVVEVSN 438 +R+RGGALPVE+ VEV N Sbjct: 439 IRVRGGALPVETTVEVPN 456 >ref|YP_001243149.1| mechanosensitive ion channel protein [Bradyrhizobium sp. BTAi1] gb|ABQ39243.1| Putative mechanosensitive ion channel protein (small-conductance (MscS) family) [Bradyrhizobium sp. BTAi1] Length = 439 Score = 637 bits (1643), Expect = e-180, Method: Compositional matrix adjust. Identities = 314/436 (72%), Positives = 373/436 (85%) Query: 1 MDMSLKDVLESIQMAARSVGAEVASPWFYLQFGIILAAAGLAYAADTAIHARVDMSSLAM 60 MD LKDVLE +Q ARS+GAEV SPWFYLQ G++L+ AG+A+A A+ AR+D++SLA+ Sbjct: 1 MDFDLKDVLELLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFATSAAVRARIDVTSLAL 60 Query: 61 RWPLPLRHFARVMVKSASTAVFAVLMIISRIVMWHATWPSRSYLIAVSAKLALAWLVIRL 120 WP PLR RV+++S +T VF +LM I+R M TWPSRSYL+AV+ KLA+AWLVIRL Sbjct: 61 GWPGPLRQLLRVLLESVTTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLAMAWLVIRL 120 Query: 121 VTSVIDNAFIVKLVSIAAWVVAALSIIGQLDWAAETLDSFAVVVGGLRLTPLLLIKAGAL 180 +TSVI N FIV++VSI+AW+VAALSIIGQLD + LDS +VV+GGLRLTPLL+IK G L Sbjct: 121 ITSVIRNTFIVQIVSISAWLVAALSIIGQLDNVVDALDSVSVVLGGLRLTPLLVIKLGVL 180 Query: 181 LIVALWLSNIASNFIDGQITRSTDLTPSIQVLLVKIIRIGLMVVAVAIALSAVGINLSAL 240 L+ LWL+N+ASNF++ +ITRSTDLTPSIQVLL+K++R+GLM++ VAIA+SAVGINLSAL Sbjct: 181 LLGVLWLTNVASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINLSAL 240 Query: 241 AVLSGAVGVGIGFGLQKIVANFISGIILLVDKSVKPGDLVTIGDSQGRISAMKTRYISVA 300 A+ SGA GVGIG GLQ+IVANF++GIILL DKSVKPGDLVTIGDS GRISAM TRYISVA Sbjct: 241 AIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYISVA 300 Query: 301 AGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKIAFAVNYDADPRLVCKLAIDTAAAHARA 360 AGDGREFLIPNEDLVTQKVTNWTYTDKNTLVK+ F NYDADPR+VCKLAI+ A R Sbjct: 301 AGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKVNFGTNYDADPRVVCKLAIEIANETPRT 360 Query: 361 LKGKPPNCILTEFAELGMKFSLTFWIADPDGMDNVKSDVMLALWDAFKQQGIRVPYPVRE 420 LK K P+C+LTEF E GMKFSLT WIADP MD+VKS+VMLALW+AFK++GIRVPYPVRE Sbjct: 361 LKSKSPSCLLTEFTEAGMKFSLTCWIADPSYMDSVKSEVMLALWEAFKREGIRVPYPVRE 420 Query: 421 LRIRGGALPVESVVEV 436 LR+RGGALPVE+ VEV Sbjct: 421 LRVRGGALPVETTVEV 436 >ref|YP_530473.1| mechanosensitive ion channel MscS [Rhodopseudomonas palustris BisB18] gb|ABD86154.1| MscS Mechanosensitive ion channel [Rhodopseudomonas palustris BisB18] Length = 440 Score = 619 bits (1595), Expect = e-175, Method: Compositional matrix adjust. Identities = 314/437 (71%), Positives = 367/437 (83%) Query: 1 MDMSLKDVLESIQMAARSVGAEVASPWFYLQFGIILAAAGLAYAADTAIHARVDMSSLAM 60 +D+ L ++ E + A RSVGAE+ SPWFY Q G+I+A AG+AYA + ARVD+S+ + Sbjct: 3 LDVKLNEIYEMLLAATRSVGAEITSPWFYFQLGLIIAGAGIAYAIGATVRARVDLSTTST 62 Query: 61 RWPLPLRHFARVMVKSASTAVFAVLMIISRIVMWHATWPSRSYLIAVSAKLALAWLVIRL 120 WP PLR RVMV SASTA FAV+M+ +R M TWPSRSYL+AV AKLALAWLVIRL Sbjct: 63 GWPAPLRLLRRVMVGSASTAAFAVVMMAARGGMLAWTWPSRSYLLAVCAKLALAWLVIRL 122 Query: 121 VTSVIDNAFIVKLVSIAAWVVAALSIIGQLDWAAETLDSFAVVVGGLRLTPLLLIKAGAL 180 VTSVI N F+V+LVS++AW VAALSI+GQL+ E LDS +VVVGGLRLTPLLLIK L Sbjct: 123 VTSVIRNGFLVRLVSLSAWCVAALSILGQLEQTIEALDSVSVVVGGLRLTPLLLIKLVVL 182 Query: 181 LIVALWLSNIASNFIDGQITRSTDLTPSIQVLLVKIIRIGLMVVAVAIALSAVGINLSAL 240 L +ALWL+N+AS F++G+I +S DLTPSIQVLL+K++R+ LM+ AV + +SAVGINLSAL Sbjct: 183 LAIALWLTNVASKFLEGRIAQSRDLTPSIQVLLIKLVRVALMIFAVTVVMSAVGINLSAL 242 Query: 241 AVLSGAVGVGIGFGLQKIVANFISGIILLVDKSVKPGDLVTIGDSQGRISAMKTRYISVA 300 A+ SGA GVGIGFGLQKIVANFISG+ILL DKSVKPGDLVTIGDS GRISAM TRYISV+ Sbjct: 243 AIFSGAAGVGIGFGLQKIVANFISGVILLADKSVKPGDLVTIGDSFGRISAMNTRYISVS 302 Query: 301 AGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKIAFAVNYDADPRLVCKLAIDTAAAHARA 360 AGDGREFLIPNEDLVTQKV NWTYTDKNT+VK+ F NYDADPRLVCKLA D AAA RA Sbjct: 303 AGDGREFLIPNEDLVTQKVVNWTYTDKNTMVKVNFGTNYDADPRLVCKLAADVAAAVPRA 362 Query: 361 LKGKPPNCILTEFAELGMKFSLTFWIADPDGMDNVKSDVMLALWDAFKQQGIRVPYPVRE 420 +K K P+C+LTEF E GMKFSLTFWIADPDGMD V+SDVML LW+AFK++GIRVPYPVRE Sbjct: 363 IKHKTPSCLLTEFTEAGMKFSLTFWIADPDGMDRVRSDVMLTLWEAFKREGIRVPYPVRE 422 Query: 421 LRIRGGALPVESVVEVS 437 +RIRGGALPVE+VVEV+ Sbjct: 423 IRIRGGALPVETVVEVA 439 >ref|YP_483680.1| MscS mechanosensitive ion channel [Rhodopseudomonas palustris HaA2] gb|ABD04769.1| MscS Mechanosensitive ion channel [Rhodopseudomonas palustris HaA2] Length = 437 Score = 610 bits (1574), Expect = e-172, Method: Compositional matrix adjust. Identities = 305/427 (71%), Positives = 363/427 (85%) Query: 10 ESIQMAARSVGAEVASPWFYLQFGIILAAAGLAYAADTAIHARVDMSSLAMRWPLPLRHF 69 E++ AARSVGAE +PWFY Q GII+ A G+ I ARVD+++L M WP+PLR F Sbjct: 9 EALLSAARSVGAEFTAPWFYAQLGIIITALGVGLGGGALIKARVDLTTLTMGWPVPLRTF 68 Query: 70 ARVMVKSASTAVFAVLMIISRIVMWHATWPSRSYLIAVSAKLALAWLVIRLVTSVIDNAF 129 RV+V SA TA+FA+ +I++R+VM HATWPSRSYLI ++AKLALAWL+IRLVTS++ NAF Sbjct: 69 LRVVVDSAWTALFALTLILARVVMLHATWPSRSYLIGIAAKLALAWLLIRLVTSILRNAF 128 Query: 130 IVKLVSIAAWVVAALSIIGQLDWAAETLDSFAVVVGGLRLTPLLLIKAGALLIVALWLSN 189 ++++VS++AW VAALSIIGQL E LDS A+VVG LRL+PLL+IK LL +ALWL+N Sbjct: 129 LIRVVSVSAWCVAALSIIGQLFPVLEALDSVAIVVGDLRLSPLLVIKLCVLLAIALWLAN 188 Query: 190 IASNFIDGQITRSTDLTPSIQVLLVKIIRIGLMVVAVAIALSAVGINLSALAVLSGAVGV 249 IASNF +G+I R+ DLTPS+QVLL+K++RIGLMV A+A+ +SAVGINL ALAV SGAVGV Sbjct: 189 IASNFFEGRINRTADLTPSVQVLLIKLMRIGLMVFAIALTMSAVGINLQALAVFSGAVGV 248 Query: 250 GIGFGLQKIVANFISGIILLVDKSVKPGDLVTIGDSQGRISAMKTRYISVAAGDGREFLI 309 GIGFGLQKI NFISG+ILL DKSVKPGDLVTIGDS G+ISAM TRYISVAAGDGREFLI Sbjct: 249 GIGFGLQKIFGNFISGVILLADKSVKPGDLVTIGDSSGKISAMNTRYISVAAGDGREFLI 308 Query: 310 PNEDLVTQKVTNWTYTDKNTLVKIAFAVNYDADPRLVCKLAIDTAAAHARALKGKPPNCI 369 PNEDL+TQKV NWTYTD+NTLVK+AFA NYDADPRLVC+LAID A ARA K KPP CI Sbjct: 309 PNEDLITQKVVNWTYTDRNTLVKVAFATNYDADPRLVCQLAIDVATNVARASKAKPPGCI 368 Query: 370 LTEFAELGMKFSLTFWIADPDGMDNVKSDVMLALWDAFKQQGIRVPYPVRELRIRGGALP 429 LTEF + GMKF+LTFWIA+PDGMD VKS+ ML LWDAFK++GIRVPYPVRE+R+RGGALP Sbjct: 369 LTEFTDTGMKFALTFWIAEPDGMDAVKSEAMLGLWDAFKREGIRVPYPVREIRVRGGALP 428 Query: 430 VESVVEV 436 VE++VEV Sbjct: 429 VETIVEV 435 >ref|YP_001202887.1| mechanosensitive ion channel protein [Bradyrhizobium sp. ORS 278] emb|CAL74647.1| Putative mechanosensitive ion channel protein (small-conductance (MscS) family) [Bradyrhizobium sp. ORS 278] Length = 442 Score = 601 bits (1550), Expect = e-170, Method: Compositional matrix adjust. Identities = 312/427 (73%), Positives = 366/427 (85%) Query: 10 ESIQMAARSVGAEVASPWFYLQFGIILAAAGLAYAADTAIHARVDMSSLAMRWPLPLRHF 69 E +Q ARS+GAEV SPWFYLQ G++L+ AG+A+AA A+ AR+D++SLAM WP PLR Sbjct: 13 EFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTSLAMGWPGPLRQL 72 Query: 70 ARVMVKSASTAVFAVLMIISRIVMWHATWPSRSYLIAVSAKLALAWLVIRLVTSVIDNAF 129 RV+++S +T VF +LM I+R M TWPSRSYL+AV+ KLALAWLVIRL+TSVI + F Sbjct: 73 LRVLLESVTTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLVIRLITSVIRSTF 132 Query: 130 IVKLVSIAAWVVAALSIIGQLDWAAETLDSFAVVVGGLRLTPLLLIKAGALLIVALWLSN 189 IV++VSI+AW+VAALSIIGQLD + LDS +VV+GGLRLTPLL+IK G LL+ LWL+N Sbjct: 133 IVQIVSISAWLVAALSIIGQLDNVVDALDSVSVVLGGLRLTPLLVIKLGVLLLAVLWLTN 192 Query: 190 IASNFIDGQITRSTDLTPSIQVLLVKIIRIGLMVVAVAIALSAVGINLSALAVLSGAVGV 249 IASNF++ +ITRSTDLTPSIQVLL+K++R+GLM++ VAIA+SAVGINLSALA+ SGA GV Sbjct: 193 IASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINLSALAIFSGAAGV 252 Query: 250 GIGFGLQKIVANFISGIILLVDKSVKPGDLVTIGDSQGRISAMKTRYISVAAGDGREFLI 309 GIG GLQ+IVANF++GIILL DKSVKPGDLVTIGDS GRISAM TRYISVAAGDGREFLI Sbjct: 253 GIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYISVAAGDGREFLI 312 Query: 310 PNEDLVTQKVTNWTYTDKNTLVKIAFAVNYDADPRLVCKLAIDTAAAHARALKGKPPNCI 369 PNEDLVTQKVTNWTYTDKNTLVK+ F NYDADPR+V KLA D AA R LK K PNC+ Sbjct: 313 PNEDLVTQKVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEMPRTLKSKAPNCL 372 Query: 370 LTEFAELGMKFSLTFWIADPDGMDNVKSDVMLALWDAFKQQGIRVPYPVRELRIRGGALP 429 LTEF E GMKFSLT WIADP MD+VKS+VMLALWDAFK++GIRVPYPVRELR+RGGALP Sbjct: 373 LTEFTEAGMKFSLTCWIADPSYMDSVKSEVMLALWDAFKREGIRVPYPVRELRVRGGALP 432 Query: 430 VESVVEV 436 VE+ VEV Sbjct: 433 VETTVEV 439