BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0155.0022.p (576 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_01999595.1| asparagine synthetase (glutamine-hydrolyzing)... 642 0.0 ref|NP_681027.1| asparagine synthetase [Thermosynechococcus elon... 642 0.0 ref|YP_001865024.1| asparagine synthase (glutamine-hydrolyzing) ... 629 e-178 ref|YP_550780.1| asparagine synthase [Polaromonas sp. JS666] >gi... 627 e-177 ref|ZP_08630734.1| glutamine-hydrolyzing asparagine synthetase [... 619 e-175 >ref|ZP_01999595.1| asparagine synthetase (glutamine-hydrolyzing) [Beggiatoa sp. PS] gb|EDN70407.1| asparagine synthetase (glutamine-hydrolyzing) [Beggiatoa sp. PS] Length = 646 Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 309/581 (53%), Positives = 418/581 (71%), Gaps = 9/581 (1%) Query: 1 MHSADGRFVMVYNGEVYSHLPIRAEIEATG--HRFRGHSDTEVLLESFARYGIRATADRL 58 M S DGRFV+V+NGE+Y++L IR E+E G +RGHSDTEV+L + +++G+ R Sbjct: 67 MLSHDGRFVIVFNGEIYNYLDIRKELEKAGVTSGWRGHSDTEVMLAAISQWGLEKAVKRF 126 Query: 59 IGMFAIAIWDRQQRTLKLIRDRLGIKPIYWAKIGGLFMFGSELKALRQHPGWTPRIEPAA 118 +GMFA A+WDR ++ L L+RDR+G KP+Y+ +G F+FGSELKALR HP W I+ A Sbjct: 127 VGMFAFALWDRDEKVLYLVRDRMGEKPLYYGWMGNTFLFGSELKALRVHPAWRGEIDKRA 186 Query: 119 VASFMRHNYIPAPHTIYRDVYKLEPGTILTLE-VGREPQTEKFWDARQVALDGLSNPLTG 177 +A MR+ YIPAP++IY+D++KL PGTIL+L + Q +W A++V G++NP G Sbjct: 187 LALLMRYCYIPAPYSIYQDIFKLLPGTILSLPFTSKSYQIRPYWSAQEVVEQGINNPYLG 246 Query: 178 NEASLVEQLETLLVDAVRKRTMADVPLGAFLSGGVDSSTVVALMKAANVGPVKTFSIGFE 237 E +EQL+ LL D+++++ +ADVPLGAFLSGG+DSSTVVALM+A + PVKTFSIGF Sbjct: 247 TEKEAIEQLDVLLRDSIKQQMIADVPLGAFLSGGIDSSTVVALMQAQSERPVKTFSIGFS 306 Query: 238 QKAFNEAPHAAAIARHLGTDHTELIVTSQEALEVVPKLSEIFDEPFADSSQIPTYLVSAM 297 + +NEA HA A+A+HLGT+HTEL VT ++A+ V+P L ++DEPFADSSQIPT+L+S + Sbjct: 307 EADYNEAQHAKAVAQHLGTEHTELYVTPKQAIAVIPNLPTLYDEPFADSSQIPTFLISQL 366 Query: 298 TRKHVTVALSGDGGDELFSGYNRYQLTRRSWRMLSLVPTPVRKAVAAGLTSVSSERWNAL 357 TR+ VTV+LSGD GDELF GY RY L + W + +P +R +A LT+VS + W+++ Sbjct: 367 TRQQVTVSLSGDAGDELFGGYKRYFLGQSLWNKVGWLPQNLRGLMAKMLTTVSPQIWDSV 426 Query: 358 FD----YLPEHSYPRLPGDKIHKFANVLGLNDADELYRKLISHWDPAGIAPGVAEARGVL 413 F LP PG K+HK A L + +E+Y + ++ W A + E VL Sbjct: 427 FSMLSPCLPSKLKLSTPGHKMHKLARFLTVTKPEEMYDEFMNLW--ANLVIADNELPTVL 484 Query: 414 WDSSVRADFPNLLDRMQFLDSVTYLPDDILTKVDRASMAVALEARVPLLDHRVVEMAWRL 473 DS+ + N + M +LD ++YLPDDIL KVDRA+M V+LE+RVP LDHR+VE AWR+ Sbjct: 485 TDSNKWDNSLNFTENMMYLDMISYLPDDILVKVDRATMGVSLESRVPFLDHRIVEFAWRI 544 Query: 474 PHSVKIRRGVSKWLLRQVLYRHVPRNLIERPKMGFTVPLDQWLRGPLRQWAENLLSEKRL 533 P S+KIR KWLLRQVLY++VP+ LIERPKMGF +PLD WLRGPL++WAENLL++KRL Sbjct: 545 PLSMKIRNNQGKWLLRQVLYQYVPKALIERPKMGFGIPLDSWLRGPLKEWAENLLAKKRL 604 Query: 534 RETDFFDVALIRRHWTEHLSRERNWQYALWDVLMFEAWRER 574 +E FF LI++ W EHLS +RNWQY LW+VLMFEAW E+ Sbjct: 605 KEEGFFHTNLIQQKWEEHLSGKRNWQYYLWNVLMFEAWLEQ 645 >ref|NP_681027.1| asparagine synthetase [Thermosynechococcus elongatus BP-1] dbj|BAC07789.1| asparagine synthetase [Thermosynechococcus elongatus BP-1] Length = 660 Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/591 (54%), Positives = 415/591 (70%), Gaps = 20/591 (3%) Query: 1 MHSADGRFVMVYNGEVYSHLPIRAEIEATGHR-----FRGHSDTEVLLESFARYGIRATA 55 M SA GR+V+ +NGE+Y+HL +R E+ G +RGHSDTE LL +FA GI T Sbjct: 67 MVSATGRYVIAFNGEIYNHLELREELSKVGAGGTAPVWRGHSDTETLLAAFAALGIEKTL 126 Query: 56 DRLIGMFAIAIWDRQQRTLKLIRDRLGIKPIYWAKIGGLFMFGSELKALRQHPGWTPRIE 115 R++GMFAIA+WDR+ RTL L RDR+G KP+Y+ + +FGSELKALR +PG+ IE Sbjct: 127 KRVVGMFAIALWDRETRTLTLARDRMGEKPLYYGWVRDALVFGSELKALRAYPGFDNAIE 186 Query: 116 PAAVASFMRHNYIPAPHTIYRDVYKLEPGTILT----------LEVGREPQTEKFWDARQ 165 ++A FMR++YIPAP +IY +++KL PG + L VG + +W R Sbjct: 187 RRSLALFMRYDYIPAPWSIYENIWKLPPGCFVQFRAASHGGEPLAVGSRGNIKPYWSVRD 246 Query: 166 VALDGLSNPLTGNEASLVEQLETLLVDAVRKRTMADVPLGAFLSGGVDSSTVVALMKAAN 225 VA GL+NP TG EA VE+LE LL ++ + MADVPLGAFLSGG+DSSTVVALM+A + Sbjct: 247 VAERGLANPFTGREADAVEELERLLRQSIAGQMMADVPLGAFLSGGIDSSTVVALMQAQS 306 Query: 226 VGPVKTFSIGFEQKAFNEAPHAAAIARHLGTDHTELIVTSQEALEVVPKLSEIFDEPFAD 285 VKTF+IGF ++ +NEA HA A+A HL T+HTEL VT QEA+ V+PKL ++DEPFAD Sbjct: 307 SRAVKTFTIGFHEQDYNEAKHAKAVACHLRTNHTELYVTPQEAMAVIPKLPSLYDEPFAD 366 Query: 286 SSQIPTYLVSAMTRKHVTVALSGDGGDELFSGYNRYQLTRRSWRMLSLVPTPVRKAVAAG 345 SSQIPT+L++ + R+ VTV+LSGDGGDELF GY RY LT W+ L+ +P P+RK G Sbjct: 367 SSQIPTFLLAQLARQQVTVSLSGDGGDELFGGYTRYFLTHSLWQRLAWIPLPMRKLAEQG 426 Query: 346 LTSVSSERWNALF----DYLPEHSYPRLPGDKIHKFANVLGLNDADELYRKLISHW-DPA 400 + ++S + WN +F LPE RLPGDK+HK A + +++Y + +SHW DP Sbjct: 427 VLAISPQTWNRVFALLAPVLPERLRFRLPGDKLHKAAALFVTRRPEDIYLRFVSHWHDPT 486 Query: 401 GIAPGVAEARGVLWDSSVRADFPNLLDRMQFLDSVTYLPDDILTKVDRASMAVALEARVP 460 + G E R L + + + + RM +LDS+TYLPDDIL KVDRA+M V+LE RVP Sbjct: 487 SLVIGTEEPRTALTEPATVLEGADFEARMMYLDSITYLPDDILVKVDRAAMGVSLETRVP 546 Query: 461 LLDHRVVEMAWRLPHSVKIRRGVSKWLLRQVLYRHVPRNLIERPKMGFTVPLDQWLRGPL 520 LLDHR+VE AW+LPH KIRRG SKWLLRQVLYR+VP+ LIERPKMGF +PLDQWLRGPL Sbjct: 547 LLDHRIVEFAWQLPHYFKIRRGQSKWLLRQVLYRYVPKELIERPKMGFGIPLDQWLRGPL 606 Query: 521 RQWAENLLSEKRLRETDFFDVALIRRHWTEHLSRERNWQYALWDVLMFEAW 571 R+WAE+LLSE+RL+ F + L+R+ W EHL RNW Y LW+VLM+EAW Sbjct: 607 REWAEDLLSEERLKREAFLNPELVRQKWQEHLLGRRNWAYHLWNVLMWEAW 657 >ref|YP_001865024.1| asparagine synthase (glutamine-hydrolyzing) [Nostoc punctiforme PCC 73102] gb|ACC80081.1| asparagine synthase (glutamine-hydrolyzing) [Nostoc punctiforme PCC 73102] Length = 649 Score = 629 bits (1623), Expect = e-178, Method: Compositional matrix adjust. Identities = 311/582 (53%), Positives = 410/582 (70%), Gaps = 12/582 (2%) Query: 1 MHSADGRFVMVYNGEVYSHLPIRAEIEATGHRFRGHSDTEVLLESFARYGIRATADRLIG 60 M SA+GR+++V+NGE+Y+ L +R E+ GH FRGHSDTE++L F+ +G+ R G Sbjct: 68 MMSANGRYMVVFNGEIYNFLELRRELTQLGHNFRGHSDTEIMLAGFSEWGLDKAIRRFNG 127 Query: 61 MFAIAIWDRQQRTLKLIRDRLGIKPIYWAKIGGLFMFGSELKALRQHPGWTPRIEPAAVA 120 MFA A+WDRQ+R L L RDRLG KP+Y+ G F+F SELKAL+ HP + I A+ Sbjct: 128 MFAFALWDRQKRILHLGRDRLGEKPLYYGWQGSTFLFTSELKALKAHPNFRAEINRDALT 187 Query: 121 SFMRHNYIPAPHTIYRDVYKLEPGTILTLE-VGREPQTEKFWDARQVALDGLSNPLTGNE 179 F R++Y+PAP++IY+ +YKL PGT+L+ + P +W AR+V G++ P G+E Sbjct: 188 QFFRYSYLPAPYSIYQGIYKLPPGTLLSWNGIDAHPDPVTYWSAREVTELGIAEPFMGSE 247 Query: 180 ASLVEQLETLLVDAVRKRTMADVPLGAFLSGGVDSSTVVALMKAANVGPVKTFSIGFEQK 239 + V QLE LL +AV R +ADVPLGAF+SGG+DSST+VALM+A + PVKTF+IGF ++ Sbjct: 248 SEAVIQLEALLREAVGLRMVADVPLGAFISGGIDSSTIVALMQAQSSQPVKTFTIGFNEE 307 Query: 240 AFNEAPHAAAIARHLGTDHTELIVTSQEALEVVPKLSEIFDEPFADSSQIPTYLVSAMTR 299 A+NEA HA A+A+HLGTDHTEL V +EALEV+PKL ++DEPF+D SQIPT+LVS + + Sbjct: 308 AYNEAKHAKAVAKHLGTDHTELYVNPKEALEVIPKLPFLYDEPFSDVSQIPTFLVSQLAK 367 Query: 300 KHVTVALSGDGGDELFSGYNRYQLTRRSWRMLSLVPTPVRKAVAAGLTSVSSERWNALF- 358 + VTV+LSGDGGDELF+GYNRY R+ W+ + +PT VRK A LTSVS + WN F Sbjct: 368 QQVTVSLSGDGGDELFAGYNRYLFGRKIWQAIGWIPTTVRKNSARALTSVSPQNWNRGFA 427 Query: 359 ---DYLPEHSYPRLPGDKIHKFANVLGLNDADELYRKLISHW-DPAGIAPGVAEARGVLW 414 LP G+K+H F+ ++ L + +Y KL+SHW DP + E +L Sbjct: 428 NVNALLPSRIKQPEFGNKLHTFSEIMALPNPTAIYTKLVSHWEDPKALVIDGLEPPTILS 487 Query: 415 DSSVRADFPNLLD---RMQFLDSVTYLPDDILTKVDRASMAVALEARVPLLDHRVVEMAW 471 +S ++P+LLD M + D+VTYLPDDIL KVDRASM V+LE R+P LDHR+VE AW Sbjct: 488 NSQ---NWPHLLDFTQCMMYFDTVTYLPDDILVKVDRASMGVSLEGRIPFLDHRLVEFAW 544 Query: 472 RLPHSVKIRRGVSKWLLRQVLYRHVPRNLIERPKMGFTVPLDQWLRGPLRQWAENLLSEK 531 RLP S+KIR G KWLLRQVLY++VP L+ERPK+GF VP++ WLRGPLR WAE LL+E Sbjct: 545 RLPLSMKIRNGQGKWLLRQVLYKYVPPTLVERPKVGFGVPINSWLRGPLRDWAEELLNEN 604 Query: 532 RLRETDFFDVALIRRHWTEHLSRERNWQYALWDVLMFEAWRE 573 RL+ FF+ IR W EHLS +W+Y LWDVLMF+AW E Sbjct: 605 RLKNEGFFNPKPIREKWEEHLSGAASWEYRLWDVLMFQAWLE 646 >ref|YP_550780.1| asparagine synthase [Polaromonas sp. JS666] gb|ABE45882.1| Asparagine synthase, glutamine-hydrolyzing [Polaromonas sp. JS666] Length = 656 Score = 627 bits (1617), Expect = e-177, Method: Compositional matrix adjust. Identities = 319/585 (54%), Positives = 407/585 (69%), Gaps = 16/585 (2%) Query: 1 MHSADGRFVMVYNGEVYSHLPIRAEIEATGHR--FRGHSDTEVLLESFARYGIRATADRL 58 MH+A GR+V+ YNGE+Y+HL +RA+++ +G +RGHSDTE LL F +GI+ +R Sbjct: 68 MHAASGRYVIAYNGEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGIQGAVERT 127 Query: 59 IGMFAIAIWDRQQRTLKLIRDRLGIKPIYWAKIG----GLFMFGSELKALRQHPGWTPRI 114 +GMFA A+WDRQ+R L L RDRLG KP+Y+ G +F+FGSELKALR HP + +I Sbjct: 128 VGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQGSGSSAVFLFGSELKALRAHPAFGAQI 187 Query: 115 EPAAVASFMRHNYIPAPHTIYRDVYKLEPGTILTLEVGR-EPQTEKFWDARQVALDGLSN 173 A+ +MRHN + H+IY+ ++KL PGT+LT+ EP +W +VA G++ Sbjct: 188 NRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEVARCGVAQ 247 Query: 174 PLTGNEASLVEQLETLLVDAVRKRTMADVPLGAFLSGGVDSSTVVALMKAANVGPVKTFS 233 P G V+ LE LL DAVR++ MADVPLGAFLSGGVDSS VVALM+ ++ PVKTFS Sbjct: 248 PFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSLRPVKTFS 307 Query: 234 IGFEQKAFNEAPHAAAIARHLGTDHTELIVTSQEALEVVPKLSEIFDEPFADSSQIPTYL 293 IGF + A+NEA HA A+A+HLGT+HTEL VT ++A+ V+P+L ++DEPFADSSQIPT+L Sbjct: 308 IGFHEAAYNEAEHAKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADSSQIPTFL 367 Query: 294 VSAMTRKHVTVALSGDGGDELFSGYNRYQLTRRSWRMLSLVPTPVRKAVAAGLTSVSSER 353 VS + R+HVTV+LSGD GDELF GYNRYQLT WR LS P P+R+A A GLT S + Sbjct: 368 VSQLARQHVTVSLSGDAGDELFGGYNRYQLTASLWRKLSAAPLPLRRAAAWGLTRFSPQS 427 Query: 354 WNALFDYLPEHSYPRLPGDKIHKFANVLGLNDADELYRKLISHW-DPAGIAPGVAEARGV 412 N+L +P + GDK+HK A V+ ++LY ++S W DPA + G E + Sbjct: 428 LNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIGGREPATL 487 Query: 413 LWDSSVRADFPNLLD--RMQFLDSVTYLPDDILTKVDRASMAVALEARVPLLDHRVVEMA 470 L R D P L D RM LD ++YLPDDILTKVDRA+M V+LE RVP LDHRVVE A Sbjct: 488 L--RGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHRVVEFA 545 Query: 471 WRLPHSVKIR----RGVSKWLLRQVLYRHVPRNLIERPKMGFTVPLDQWLRGPLRQWAEN 526 WRLP K+R R +KW LRQVLYRHVP+ LI+RPKMGF VP+D+WLRGPLR WAE+ Sbjct: 546 WRLPLHYKLRQEGGRYTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLRDWAED 605 Query: 527 LLSEKRLRETDFFDVALIRRHWTEHLSRERNWQYALWDVLMFEAW 571 LLSE RL+ +F A IR+ W EHL +RNW LW LMF+AW Sbjct: 606 LLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPHLWTALMFQAW 650 >ref|ZP_08630734.1| glutamine-hydrolyzing asparagine synthetase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP06588.1| glutamine-hydrolyzing asparagine synthetase [Bradyrhizobiaceae bacterium SG-6C] Length = 644 Score = 619 bits (1596), Expect = e-175, Method: Compositional matrix adjust. Identities = 321/576 (55%), Positives = 401/576 (69%), Gaps = 3/576 (0%) Query: 1 MHSADGRFVMVYNGEVYSHLPIRAEIEATGHRFRGHSDTEVLLESFARYGIRATADRLIG 60 M S+DGR+V+ YNGE+Y+ IR E +RGHSDTE L+ES A G+ AT D L G Sbjct: 68 MVSSDGRWVICYNGEIYNSDDIRREPALHAVAWRGHSDTETLVESVAHRGLEATLDDLNG 127 Query: 61 MFAIAIWDRQQRTLKLIRDRLGIKPIYWAKIGGLFMFGSELKALRQHPGWTPRIEPAAVA 120 MFAIA+WDR++R+L LIRDRLGIKP++ A+ G F SELK+L ++ IEPA+VA Sbjct: 128 MFAIALWDRKERSLHLIRDRLGIKPLFIARQGDRIYFASELKSLHAARAFSLEIEPASVA 187 Query: 121 SFMRHNYIPAPHTIYRDVYKLEPGTILTLEVGREPQTEKFWDARQVALDGLSNPLTGNEA 180 S +R Y+PAPHTIYR V KL PG T +G + ++W A VA GL++PL ++ Sbjct: 188 SLLRFGYVPAPHTIYRGVEKLMPGEHATFSIGGIYSSRRYWSAADVAERGLASPLEISDE 247 Query: 181 SLVEQLETLLVDAVRKRTMADVPLGAFLSGGVDSSTVVALMKAANVGPVKTFSIGFEQKA 240 + QL TLL DAV ++ +ADVP+GAFLSGG+DSS V LM AA G V+TFSIGF + Sbjct: 248 EAIVQLSTLLADAVSRQMLADVPIGAFLSGGIDSSAVAGLMVAAKRGAVRTFSIGFPEFG 307 Query: 241 FNEAPHAAAIARHLGTDHTELIVTSQEALEVVPKLSEIFDEPFADSSQIPTYLVSAMTRK 300 F+E+ AAA+ARHLGT+HTEL+VT+ +AL VVP+L++++DEPFADSSQIPT+LVS +TR+ Sbjct: 308 FDESKDAAAVARHLGTEHTELVVTASDALGVVPQLAQMYDEPFADSSQIPTHLVSKLTRQ 367 Query: 301 HVTVALSGDGGDELFSGYNRYQLTRRSWRMLSLVPTPVRKAVAAGLTSVSSERWNALFDY 360 HVTVALSGDGGDELF GYNRY+L + L VP VR+ A L +V ++L Sbjct: 368 HVTVALSGDGGDELFCGYNRYRLAEGMSQNLGRVPLGVRRVAAGVLNAVPMGWVDSLASL 427 Query: 361 LPEHSYPRLPGDKIHKFANVLGLNDADELYRKLISHW-DPAGIAPGVAEARGVLWDSSVR 419 P P DK+ KFA+V+ L D + +YR+L+S +PA +AP E W + Sbjct: 428 APRGMLPSQTADKLRKFASVMTL-DPEGIYRRLVSQCENPAELAPLAGEHPLKPWSTGAV 486 Query: 420 ADFPNLLDRMQFLDSVTYLPDDILTKVDRASMAVALEARVPLLDHRVVEMAWRLPHSVKI 479 P LDRMQ LD++TYLPDDIL KVDRASMAVALE R PLLDHRVVE AWRLP K Sbjct: 487 GALPTFLDRMQLLDTMTYLPDDILQKVDRASMAVALEVRPPLLDHRVVEFAWRLPRRFKT 546 Query: 480 RRGVSKWLLRQVLYRHVPRNLIERPKMGFTVPLDQWLRGPLRQWAENLLSEKRLRETDFF 539 R G +KWLLR+V R VPR L+ERPKMGF VPL WLRGPL++WAE+LL ++L F Sbjct: 547 RNGETKWLLRRVTERMVPRQLLERPKMGFGVPLADWLRGPLKEWAEDLLDPQQL-GAGFI 605 Query: 540 DVALIRRHWTEHLSRERNWQYALWDVLMFEAWRERW 575 DVA RR W EHL+ RNW YALW +LMFEAWR RW Sbjct: 606 DVAAARRLWAEHLTGRRNWAYALWTILMFEAWRRRW 641