BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0152.0066.p (365 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002498599.1| pyruvate dehydrogenase (acetyl-transferring)... 636 e-180 ref|YP_578306.1| pyruvate dehydrogenase (lipoamide) [Nitrobacter... 566 e-159 ref|YP_003609326.1| pyruvate dehydrogenase (acetyl-transferring)... 424 e-117 ref|YP_158234.1| putative pyruvate dehydrogenase E1 component (a... 424 e-116 ref|ZP_02377216.1| probable pyruvate dehydrogenase e1 component ... 419 e-115 >ref|YP_002498599.1| pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha [Methylobacterium nodulans ORS 2060] gb|ACL58296.1| pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit [Methylobacterium nodulans ORS 2060] Length = 365 Score = 636 bits (1641), Expect = e-180, Method: Compositional matrix adjust. Identities = 321/365 (87%), Positives = 340/365 (93%) Query: 1 VASKRANEELPVIARFEVRRRNYLAPDGSIERPLPAFATDAKLLVELYRSMVLLRLFDRK 60 +AS+R N++LPV+ARFEVR R YLAPDGSI RPLPAFA+DA LL+ LYR+MVLLRLFD+K Sbjct: 1 MASRRVNQDLPVVARFEVRHRKYLAPDGSIARPLPAFASDANLLIALYRAMVLLRLFDKK 60 Query: 61 AVALQRTGRLGTYAISLGQEAVSVGVASAMREDDVLLPSYRDNGALLWRGVKLEEILLFW 120 AVALQRTGRLGTYA+SLGQEAVSV +ASAMRE+DVLLPSYRDNGALLWRGVKLEEILLFW Sbjct: 61 AVALQRTGRLGTYAVSLGQEAVSVAIASAMREEDVLLPSYRDNGALLWRGVKLEEILLFW 120 Query: 121 GGDERGNDFSGPRHDFPFCVPVGSQAPHAAGVAYALKLRKEPRVAVCMFGDGATSKGDVY 180 GGDERGN FSGP HDFPFCVPVGSQAPHAAGVAYALKLRK+P VAVC+FGDGATSKGDVY Sbjct: 121 GGDERGNCFSGPVHDFPFCVPVGSQAPHAAGVAYALKLRKKPHVAVCLFGDGATSKGDVY 180 Query: 181 EAMNFAGVHKLPLVFVATNNQWAISVPLRLQTGSETLAQKAIGAGFSGEQVDGSDVVAMR 240 EAMNFAGVHKLP+VFV TNNQWAISVPLRLQT SETLAQKAI AGF+GEQVDGSDVVAMR Sbjct: 181 EAMNFAGVHKLPVVFVTTNNQWAISVPLRLQTSSETLAQKAIAAGFTGEQVDGSDVVAMR 240 Query: 241 AAAEEAIAAARDGKGPHFIEAVTYRLGDHTTSDDALRYRSAEEVQAHWKEEPIARLRSYL 300 AAAEEAIAAARDGKGP FIEAVTYRLGDHTTSDDA RYRSA+EVQA WKEEPIARLR+YL Sbjct: 241 AAAEEAIAAARDGKGPRFIEAVTYRLGDHTTSDDASRYRSADEVQARWKEEPIARLRAYL 300 Query: 301 VGQKMWTKADEERLAAECHERIEAAVERYLKTPPRRAETMFDNLYADLPEAYAAQRRELA 360 V QKMW KADEERLA ECHERIEAAVERYL T PRR ETMFD+LYADLPE YAAQRREL Sbjct: 301 VAQKMWGKADEERLATECHERIEAAVERYLATAPRRPETMFDHLYADLPEVYAAQRRELT 360 Query: 361 GEDDA 365 GE DA Sbjct: 361 GEHDA 365 >ref|YP_578306.1| pyruvate dehydrogenase (lipoamide) [Nitrobacter hamburgensis X14] gb|ABE63846.1| Pyruvate dehydrogenase (lipoamide) [Nitrobacter hamburgensis X14] Length = 363 Score = 566 bits (1458), Expect = e-159, Method: Compositional matrix adjust. Identities = 283/353 (80%), Positives = 309/353 (87%) Query: 7 NEELPVIARFEVRRRNYLAPDGSIERPLPAFATDAKLLVELYRSMVLLRLFDRKAVALQR 66 + +LPVIARFEVR RNYLAPDG+I RP PAFA+D LLV LYR+MVL+R FD KAVALQR Sbjct: 7 DNQLPVIARFEVRYRNYLAPDGAITRPPPAFASDIGLLVSLYRAMVLVRAFDLKAVALQR 66 Query: 67 TGRLGTYAISLGQEAVSVGVASAMREDDVLLPSYRDNGALLWRGVKLEEILLFWGGDERG 126 TGRLGTYA+SLGQEAV+VGVASAMR++DVLLPSYRDN A++WRGVKLEEILLFWGGDERG Sbjct: 67 TGRLGTYAVSLGQEAVAVGVASAMRDEDVLLPSYRDNAAMIWRGVKLEEILLFWGGDERG 126 Query: 127 NDFSGPRHDFPFCVPVGSQAPHAAGVAYALKLRKEPRVAVCMFGDGATSKGDVYEAMNFA 186 N SGP HDFP CVPVGSQAPHAAGVAYA KLR+EPRVAVC+FGDGATSKGDV+EAMNFA Sbjct: 127 NHSSGPVHDFPCCVPVGSQAPHAAGVAYAFKLRREPRVAVCLFGDGATSKGDVFEAMNFA 186 Query: 187 GVHKLPLVFVATNNQWAISVPLRLQTGSETLAQKAIGAGFSGEQVDGSDVVAMRAAAEEA 246 GVHKLP+VFV TNNQWAISVPLRLQT SETLAQKAI AGF GEQVDG+D VAM AAA EA Sbjct: 187 GVHKLPVVFVITNNQWAISVPLRLQTASETLAQKAIAAGFIGEQVDGNDAVAMHAAATEA 246 Query: 247 IAAARDGKGPHFIEAVTYRLGDHTTSDDALRYRSAEEVQAHWKEEPIARLRSYLVGQKMW 306 IAAARDG+GP IEAVTYRLGDHTT+DDA RYR EEVQA WK+EPI RLR+YLVG+ W Sbjct: 247 IAAARDGRGPRLIEAVTYRLGDHTTADDAARYRPPEEVQARWKDEPIGRLRAYLVGRNAW 306 Query: 307 TKADEERLAAECHERIEAAVERYLKTPPRRAETMFDNLYADLPEAYAAQRREL 359 KA EE+L AEC RI AAVERYL T PR ETMFD+LYA LP YAAQRR++ Sbjct: 307 DKAKEEQLVAECQTRIAAAVERYLATGPRAPETMFDHLYATLPPVYAAQRRDV 359 >ref|YP_003609326.1| pyruvate dehydrogenase (acetyl-transferring) E1 component, subunit alpha [Burkholderia sp. CCGE1002] gb|ADG19815.1| pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit [Burkholderia sp. CCGE1002] Length = 371 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 205/346 (59%), Positives = 248/346 (71%) Query: 10 LPVIARFEVRRRNYLAPDGSIERPLPAFATDAKLLVELYRSMVLLRLFDRKAVALQRTGR 69 + A F + YL P+ +PLPAFA + + L+ LYR+MVL R FD KAVALQRTG+ Sbjct: 1 MTTTASFHIGYTRYLGPESEPVQPLPAFAREPEALISLYRAMVLTRAFDTKAVALQRTGK 60 Query: 70 LGTYAISLGQEAVSVGVASAMREDDVLLPSYRDNGALLWRGVKLEEILLFWGGDERGNDF 129 LGT+A S+GQEA+ VGVASAM+ DDVL PSYRD+ A L RGV + E LL+WGGDERG+DF Sbjct: 61 LGTFASSVGQEAIGVGVASAMQADDVLFPSYRDHAAQLLRGVTMTESLLYWGGDERGSDF 120 Query: 130 SGPRHDFPFCVPVGSQAPHAAGVAYALKLRKEPRVAVCMFGDGATSKGDVYEAMNFAGVH 189 S PR DFP CVP+G+Q HAAG AYA KLR EPRVAV + GDG+TSKGD YEAMN AGV Sbjct: 121 SVPRFDFPNCVPIGTQVCHAAGAAYAFKLRGEPRVAVAICGDGSTSKGDFYEAMNMAGVW 180 Query: 190 KLPLVFVATNNQWAISVPLRLQTGSETLAQKAIGAGFSGEQVDGSDVVAMRAAAEEAIAA 249 + PLV V NNQWAISVP Q+ ++TLAQKAI AG G QVDG+DV+A+ A+A Sbjct: 181 QTPLVLVVNNNQWAISVPRSTQSAAQTLAQKAIAAGIDGLQVDGNDVIAVHQVMHAALAK 240 Query: 250 ARDGKGPHFIEAVTYRLGDHTTSDDALRYRSAEEVQAHWKEEPIARLRSYLVGQKMWTKA 309 AR G GP IEA++YRLGDHTT+DDA RYR ++E+Q W+ EP+ RLR YL+ W KA Sbjct: 241 ARRGDGPTLIEALSYRLGDHTTADDATRYRDSDEIQKQWEYEPLLRLRKYLMRMNFWDKA 300 Query: 310 DEERLAAECHERIEAAVERYLKTPPRRAETMFDNLYADLPEAYAAQ 355 +E+L CH ++EAAVE YL PP MFD+LY LP A Q Sbjct: 301 QDEQLGRACHAQVEAAVEAYLAVPPPDTSAMFDHLYETLPHAMREQ 346 >ref|YP_158234.1| putative pyruvate dehydrogenase E1 component (alpha subunit) oxidoreductase protein [Aromatoleum aromaticum EbN1] emb|CAI07333.1| putative pyruvate dehydrogenase E1 component (alpha subunit) oxidoreductase protein [Aromatoleum aromaticum EbN1] Length = 362 Score = 424 bits (1091), Expect = e-116, Method: Compositional matrix adjust. Identities = 207/347 (59%), Positives = 250/347 (72%) Query: 10 LPVIARFEVRRRNYLAPDGSIERPLPAFATDAKLLVELYRSMVLLRLFDRKAVALQRTGR 69 + I RF+V +L P G +PLP FA D + L+ LYR+MVL R FD KA+ALQRTG+ Sbjct: 1 MTTIGRFQVDYTRFLDPRGEATQPLPDFALDPEKLIPLYRAMVLTRTFDTKAIALQRTGK 60 Query: 70 LGTYAISLGQEAVSVGVASAMREDDVLLPSYRDNGALLWRGVKLEEILLFWGGDERGNDF 129 LGT+A +LGQEA+ VGVA+AMR +DVL+PSYRD+ A L RGV + E LL+WGGDERG+DF Sbjct: 61 LGTFASALGQEAIGVGVAAAMRAEDVLVPSYRDHPAQLLRGVTMAESLLYWGGDERGSDF 120 Query: 130 SGPRHDFPFCVPVGSQAPHAAGVAYALKLRKEPRVAVCMFGDGATSKGDVYEAMNFAGVH 189 +GPR DFP CVP+ +Q HAAG AYA +LR+E RVAVC GDG TSKGD YEA+NF V Sbjct: 121 AGPRDDFPICVPIATQVCHAAGAAYAFQLRQEARVAVCFIGDGGTSKGDFYEALNFGAVW 180 Query: 190 KLPLVFVATNNQWAISVPLRLQTGSETLAQKAIGAGFSGEQVDGSDVVAMRAAAEEAIAA 249 K PLV V NNQWAISVP +Q+ ++TLAQKAI AG G QVDG+D+VA+ AA +AI Sbjct: 181 KAPLVVVVNNNQWAISVPRSMQSAAQTLAQKAIAAGIEGCQVDGNDIVAVHHAARQAIEK 240 Query: 250 ARDGKGPHFIEAVTYRLGDHTTSDDALRYRSAEEVQAHWKEEPIARLRSYLVGQKMWTKA 309 AR G GP IEA++YRLGDHTT+DDA RYR VQ +W EP+ RLR++LV W Sbjct: 241 ARSGGGPSLIEALSYRLGDHTTADDASRYRDPAVVQENWTFEPVVRLRNHLVRLNAWDPQ 300 Query: 310 DEERLAAECHERIEAAVERYLKTPPRRAETMFDNLYADLPEAYAAQR 356 EE+L EC E + AAVERYL TPP E MFD+LY LP A QR Sbjct: 301 HEEQLVRECGEAVNAAVERYLATPPPTPEAMFDHLYRTLPAALQEQR 347 >ref|ZP_02377216.1| probable pyruvate dehydrogenase e1 component (alpha subunit) oxidoreductase protein [Burkholderia ubonensis Bu] Length = 362 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 202/342 (59%), Positives = 243/342 (71%) Query: 10 LPVIARFEVRRRNYLAPDGSIERPLPAFATDAKLLVELYRSMVLLRLFDRKAVALQRTGR 69 + +ARF V YL PDG +PLP FA D L+ LYR+MVL R FD KAVALQRTG+ Sbjct: 1 MSTVARFHVDYTQYLGPDGEPVQPLPEFAQDPAALLPLYRAMVLTRAFDAKAVALQRTGK 60 Query: 70 LGTYAISLGQEAVSVGVASAMREDDVLLPSYRDNGALLWRGVKLEEILLFWGGDERGNDF 129 LGT+A S+GQEA+ VGVASAM+ DDVL PSYRD+ A L RGV + E LL+WGGDERG+DF Sbjct: 61 LGTFASSVGQEAIGVGVASAMQTDDVLFPSYRDHAAQLLRGVTMAESLLYWGGDERGSDF 120 Query: 130 SGPRHDFPFCVPVGSQAPHAAGVAYALKLRKEPRVAVCMFGDGATSKGDVYEAMNFAGVH 189 RHDFP CVP+G+Q HAAG AYA LR+EPRVAV +FGDG TSKGD YEAMN AG Sbjct: 121 GAARHDFPNCVPIGTQVCHAAGAAYAFMLRREPRVAVTIFGDGGTSKGDFYEAMNMAGAW 180 Query: 190 KLPLVFVATNNQWAISVPLRLQTGSETLAQKAIGAGFSGEQVDGSDVVAMRAAAEEAIAA 249 + PLV V +NQWAISVP QT ++TLAQKAI AG G QVDG+DV+A+R EA+ Sbjct: 181 QAPLVLVINDNQWAISVPRTRQTAAQTLAQKAIAAGIEGRQVDGNDVIAVRQVVSEALDK 240 Query: 250 ARDGKGPHFIEAVTYRLGDHTTSDDALRYRSAEEVQAHWKEEPIARLRSYLVGQKMWTKA 309 AR G GP +EA++YRLGDHTT+DDA RYR A+ V W+ EP+ RLR+YL W KA Sbjct: 241 ARRGGGPTLVEALSYRLGDHTTADDATRYRDADTVAKQWEHEPLIRLRTYLTRMNAWDKA 300 Query: 310 DEERLAAECHERIEAAVERYLKTPPRRAETMFDNLYADLPEA 351 + L C+ +++AAV+ YL P MFD+LYA LP A Sbjct: 301 RDTELGKACYAQVDAAVQEYLAVAPPGTSAMFDHLYATLPRA 342