BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0146.0020.p (397 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_770995.1| DNA topoisomerase IV subunit B [Bradyrhizobium ... 702 0.0 ref|YP_318266.1| DNA topoisomerase IV subunit B [Nitrobacter win... 699 0.0 ref|YP_001991749.1| DNA topoisomerase IV subunit B [Rhodopseudom... 692 0.0 ref|ZP_01047276.1| gram negative topoisomerase IV, subunit B [Ni... 690 0.0 ref|YP_569615.1| DNA topoisomerase IV subunit B [Rhodopseudomona... 686 0.0 >ref|NP_770995.1| DNA topoisomerase IV subunit B [Bradyrhizobium japonicum USDA 110] dbj|BAC49620.1| DNA gyrase subunit B [Bradyrhizobium japonicum USDA 110] Length = 681 Score = 702 bits (1813), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/378 (89%), Positives = 356/378 (94%), Gaps = 1/378 (0%) Query: 5 LKSNVKSKSADDLFGAPEPKGRAPAKAASRSAGGAEAGYTAADIEVLEGLEPVRRRPGMY 64 + +K K+ DD FG EPK RA AKA+ R AGGAEA YTAADIEVLEGLEPVRRRPGMY Sbjct: 1 MSKQLKPKAKDDFFGGNEPKPRAAAKASPR-AGGAEADYTAADIEVLEGLEPVRRRPGMY 59 Query: 65 IGGTDEKALHHLFAEVIDNCMDEALAGHATFIDVELTADGFLTVTDNGRGIPVDPHPKFP 124 IGGTDEKALHHLFAEVIDN MDEALAGHATFI VEL+ADGFLTVTDNGRGIP+DPHPKFP Sbjct: 60 IGGTDEKALHHLFAEVIDNSMDEALAGHATFIGVELSADGFLTVTDNGRGIPIDPHPKFP 119 Query: 125 KKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSGQLHRMTFE 184 KKSALEVIMCTLHSGGKFDSKVYETSGGLHGVG+SVVNALSS LEVEVARS +L+RMTFE Sbjct: 120 KKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGISVVNALSSLLEVEVARSQKLYRMTFE 179 Query: 185 RGHPKGKLEDLGKVNNRRGTRIRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGVEIRW 244 RGHPKGKLEDLGK+NNRRGTR+RFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGVEIRW Sbjct: 180 RGHPKGKLEDLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGVEIRW 239 Query: 245 HCDQELLKGVEDVPAEAKFHFPGGLKDYLAAAIHADTLVHQDIFSGKSGRSGAHGACEWA 304 +C ELLKG++DVPAEA FHFPGGLKDYLAAAIHADTLVH DIFSGKSGR+GAHGACEWA Sbjct: 240 NCAPELLKGIDDVPAEATFHFPGGLKDYLAAAIHADTLVHPDIFSGKSGRNGAHGACEWA 299 Query: 305 VAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRAASITSEDVM 364 VAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAER GQGKRA+SITSEDVM Sbjct: 300 VAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERVGQGKRASSITSEDVM 359 Query: 365 VGAAVMLSVFVREPEFQG 382 VGAAVMLSVFVREPEFQG Sbjct: 360 VGAAVMLSVFVREPEFQG 377 >ref|YP_318266.1| DNA topoisomerase IV subunit B [Nitrobacter winogradskyi Nb-255] gb|ABA04914.1| DNA topoisomerase IV subunit B [Nitrobacter winogradskyi Nb-255] Length = 685 Score = 699 bits (1804), Expect = 0.0, Method: Compositional matrix adjust. Identities = 341/382 (89%), Positives = 352/382 (92%), Gaps = 1/382 (0%) Query: 1 MAKSLKSNVKSKSADDLFGAPEPKGRAPAKAASRSAGGAEAGYTAADIEVLEGLEPVRRR 60 M KSLKSN+K K ADDLF A E KGR+ A+A R A GAEA YTAADIEVLEGLEPVRRR Sbjct: 1 MVKSLKSNIKKKQADDLFAASETKGRSSARAPGR-ASGAEADYTAADIEVLEGLEPVRRR 59 Query: 61 PGMYIGGTDEKALHHLFAEVIDNCMDEALAGHATFIDVELTADGFLTVTDNGRGIPVDPH 120 PGMYIGGTDEKALHHLFAEVIDN MDEALAGHATFI VEL ADGFLTVTDNGRGIPVDPH Sbjct: 60 PGMYIGGTDEKALHHLFAEVIDNAMDEALAGHATFIVVELAADGFLTVTDNGRGIPVDPH 119 Query: 121 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSGQLHR 180 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSS LEVEVARS L+R Sbjct: 120 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSHLEVEVARSQNLYR 179 Query: 181 MTFERGHPKGKLEDLGKVNNRRGTRIRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGV 240 MTFERGHPKGKLEDLG++ NRRGT IRFKPDT+IFGAKAAFKPQRLFKMTRSKAYLFGGV Sbjct: 180 MTFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGV 239 Query: 241 EIRWHCDQELLKGVEDVPAEAKFHFPGGLKDYLAAAIHADTLVHQDIFSGKSGRSGAHGA 300 EIRW C ELLKG+ED+P EA FHFPGGLKDYLAAAIH DTLVH DIFSG+SGR+GAHGA Sbjct: 240 EIRWRCAPELLKGLEDLPPEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGRSGRNGAHGA 299 Query: 301 CEWAVAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRAASITS 360 CEWAVAWTADADGFLSSY NTVPTPDGGTHESGLRSALLRGLKDHAER GQGKRA SITS Sbjct: 300 CEWAVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERVGQGKRAGSITS 359 Query: 361 EDVMVGAAVMLSVFVREPEFQG 382 EDVMVGAAVMLSVFVREPEFQG Sbjct: 360 EDVMVGAAVMLSVFVREPEFQG 381 >ref|YP_001991749.1| DNA topoisomerase IV subunit B [Rhodopseudomonas palustris TIE-1] gb|ACF01274.1| DNA topoisomerase IV, B subunit [Rhodopseudomonas palustris TIE-1] Length = 683 Score = 692 bits (1786), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/384 (88%), Positives = 357/384 (92%), Gaps = 7/384 (1%) Query: 1 MAKSLKSNVKSKSADDLF-GAPEPKG-RAPAKAASRSAGGAEAGYTAADIEVLEGLEPVR 58 MAK+LK++ KSKS DDLF GAP+ +APAK + GAE GYTAADIEVLEGLEPVR Sbjct: 1 MAKALKASSKSKSGDDLFEGAPKSAAPKAPAKPS-----GAEDGYTAADIEVLEGLEPVR 55 Query: 59 RRPGMYIGGTDEKALHHLFAEVIDNCMDEALAGHATFIDVELTADGFLTVTDNGRGIPVD 118 RRPGMYIGGTDEKALHHLFAEVIDN MDEALAGHATFI+VELTADGFLTV+DNGRGIPVD Sbjct: 56 RRPGMYIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELTADGFLTVSDNGRGIPVD 115 Query: 119 PHPKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSGQL 178 PHPKFPKKSALEVIMCTLH+GGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVAR QL Sbjct: 116 PHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARGQQL 175 Query: 179 HRMTFERGHPKGKLEDLGKVNNRRGTRIRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFG 238 HRM FERGHPKGKLE LGKVNNRRGTR+RFKPDTDIFGAKAAFKPQRLFKM RSKAYLFG Sbjct: 176 HRMVFERGHPKGKLESLGKVNNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFG 235 Query: 239 GVEIRWHCDQELLKGVEDVPAEAKFHFPGGLKDYLAAAIHADTLVHQDIFSGKSGRSGAH 298 GV+IRW CD ELLKG+EDVPAE +FHFPGGLKD+L AAIH DTLVH DIF+GKSGR+GAH Sbjct: 236 GVKIRWKCDPELLKGIEDVPAEDEFHFPGGLKDFLGAAIHNDTLVHPDIFAGKSGRNGAH 295 Query: 299 GACEWAVAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRAASI 358 GACEWAVAWTADADGFLSSY NTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRA+SI Sbjct: 296 GACEWAVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRASSI 355 Query: 359 TSEDVMVGAAVMLSVFVREPEFQG 382 TSEDVMVGAA+MLSVFVREPEFQG Sbjct: 356 TSEDVMVGAAIMLSVFVREPEFQG 379 >ref|ZP_01047276.1| gram negative topoisomerase IV, subunit B [Nitrobacter sp. Nb-311A] gb|EAQ34704.1| gram negative topoisomerase IV, subunit B [Nitrobacter sp. Nb-311A] Length = 681 Score = 690 bits (1780), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/382 (87%), Positives = 350/382 (91%), Gaps = 5/382 (1%) Query: 1 MAKSLKSNVKSKSADDLFGAPEPKGRAPAKAASRSAGGAEAGYTAADIEVLEGLEPVRRR 60 M KSLK N K KS++DLF A + KG PA+A+ GAE GYTAADIEVLEGLEPVRRR Sbjct: 1 MPKSLKQNTKVKSSNDLFSASKAKGHPPARAS-----GAETGYTAADIEVLEGLEPVRRR 55 Query: 61 PGMYIGGTDEKALHHLFAEVIDNCMDEALAGHATFIDVELTADGFLTVTDNGRGIPVDPH 120 PGMYIGGTDEKALHHLFAEVIDN MDEALAGHATFI VEL DGFLTVTDNGRGIPVDPH Sbjct: 56 PGMYIGGTDEKALHHLFAEVIDNAMDEALAGHATFIVVELATDGFLTVTDNGRGIPVDPH 115 Query: 121 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSGQLHR 180 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARS L++ Sbjct: 116 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSQNLYK 175 Query: 181 MTFERGHPKGKLEDLGKVNNRRGTRIRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGV 240 MTFERGHPKGKLEDLG++ NRRGT IRFKPDT+IFGAKAAFKPQRLFKMTRSKAYLFGGV Sbjct: 176 MTFERGHPKGKLEDLGRIANRRGTTIRFKPDTEIFGAKAAFKPQRLFKMTRSKAYLFGGV 235 Query: 241 EIRWHCDQELLKGVEDVPAEAKFHFPGGLKDYLAAAIHADTLVHQDIFSGKSGRSGAHGA 300 EIRW C ELLKG++D+PAEA FHFPGGLKDYLAAAIH DTLVH DIFSGKSGR+GAHGA Sbjct: 236 EIRWRCAPELLKGLDDIPAEATFHFPGGLKDYLAAAIHKDTLVHPDIFSGKSGRNGAHGA 295 Query: 301 CEWAVAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRAASITS 360 CEWAVAWTADADGFLSSY NTVPTPDGGTHESGLRSALLRGLKDHAER G GKRAASITS Sbjct: 296 CEWAVAWTADADGFLSSYCNTVPTPDGGTHESGLRSALLRGLKDHAERVGYGKRAASITS 355 Query: 361 EDVMVGAAVMLSVFVREPEFQG 382 EDVMVGAAVMLSVFVREPEFQG Sbjct: 356 EDVMVGAAVMLSVFVREPEFQG 377 >ref|YP_569615.1| DNA topoisomerase IV subunit B [Rhodopseudomonas palustris BisB5] gb|ABE39714.1| DNA topoisomerase IV subunit B [Rhodopseudomonas palustris BisB5] Length = 684 Score = 686 bits (1771), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/382 (87%), Positives = 352/382 (92%), Gaps = 2/382 (0%) Query: 1 MAKSLKSNVKSKSADDLFGAPEPKGRAPAKAASRSAGGAEAGYTAADIEVLEGLEPVRRR 60 MAK+LK + KSKSA DLFGA PK AP + A++ GGAEAGYTAADIEVLEGLEPVRRR Sbjct: 1 MAKALKPSSKSKSAGDLFGAA-PKSAAP-RPAAKPTGGAEAGYTAADIEVLEGLEPVRRR 58 Query: 61 PGMYIGGTDEKALHHLFAEVIDNCMDEALAGHATFIDVELTADGFLTVTDNGRGIPVDPH 120 PGMYIGGTDEKALHHLFAEVIDN MDEALAGHATFI+VELT DGFLTV+DNGRGIPVDPH Sbjct: 59 PGMYIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELTTDGFLTVSDNGRGIPVDPH 118 Query: 121 PKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVVNALSSRLEVEVARSGQLHR 180 PKFPKKSALEVIMCTLH+GGKFDSKVYETSGGLHGVGVSVVNALSS LEVEVAR QL+R Sbjct: 119 PKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSLLEVEVARGQQLYR 178 Query: 181 MTFERGHPKGKLEDLGKVNNRRGTRIRFKPDTDIFGAKAAFKPQRLFKMTRSKAYLFGGV 240 M F RG P+GKLE LGK+NNRRGTR+RFKPD DIFGAKAAFKPQRLFKM RSKAYLFGGV Sbjct: 179 MVFARGIPQGKLESLGKINNRRGTRVRFKPDADIFGAKAAFKPQRLFKMARSKAYLFGGV 238 Query: 241 EIRWHCDQELLKGVEDVPAEAKFHFPGGLKDYLAAAIHADTLVHQDIFSGKSGRSGAHGA 300 EIRW CD ELLKG+EDVPAE FHFPGGLKDYL AAIHADTLVH DIFSGKSGR+GAHGA Sbjct: 239 EIRWKCDPELLKGLEDVPAEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGA 298 Query: 301 CEWAVAWTADADGFLSSYTNTVPTPDGGTHESGLRSALLRGLKDHAERAGQGKRAASITS 360 CEWAVAWTADADGFLSSY NTVPTPDGGTHESG+RSALLRGLKDHAER GQGKRA+SITS Sbjct: 299 CEWAVAWTADADGFLSSYCNTVPTPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITS 358 Query: 361 EDVMVGAAVMLSVFVREPEFQG 382 EDVMVGAAVMLSVFVREPEFQG Sbjct: 359 EDVMVGAAVMLSVFVREPEFQG 380