BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0144.0015.p (287 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767058.1| epoxide hydrolase [Bradyrhizobium japonicum USD... 473 e-131 ref|YP_001924150.1| alpha/beta hydrolase fold protein [Methyloba... 359 3e-97 ref|YP_001638925.1| alpha/beta hydrolase fold protein [Methyloba... 358 6e-97 ref|YP_002962483.1| alpha/beta hydrolase, epoxide hydrolase [met... 357 1e-96 ref|YP_003067666.1| alpha/beta hydrolase [Methylobacterium extor... 353 2e-95 >ref|NP_767058.1| epoxide hydrolase [Bradyrhizobium japonicum USDA 110] dbj|BAC45683.1| blr0418 [Bradyrhizobium japonicum USDA 110] Length = 322 Score = 473 bits (1218), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/287 (78%), Positives = 246/287 (85%) Query: 1 MEHVTIRANGAAFHVARTGAGRPLLLLHGWPEFWLTWKPVMARLADRYTLIAPDLRGFGD 60 MEH+TI+ANGA FH+ R G G+PLLLLHGWPEFWLTW+PVM RLADR+ LIAPDLRGFGD Sbjct: 36 MEHLTIKANGANFHLVRAGKGKPLLLLHGWPEFWLTWEPVMVRLADRFMLIAPDLRGFGD 95 Query: 61 SDKPHGAYGPDQHTDDMLALLDALGVDKAGVVGHDVGGAVMQPLARKAPDRIAGLFLFDF 120 SDKP G YGPD H DMLAL++ LG+++ VVGHDVGGAVMQPLAR+AP R+AGLF FDF Sbjct: 96 SDKPDGPYGPDGHAADMLALMEGLGIERFAVVGHDVGGAVMQPLARQAPGRLAGLFFFDF 155 Query: 121 VYPGIGPRMAAPDRLNHIWYQSFHQMEMAPALVGASRDSCRTYIRHFLHNWSHRKAAFDD 180 VYPGIGPRMAAPDRLNHIWYQSFHQMEMA LVGASR+SCR YI HFL W+HRK AFDD Sbjct: 156 VYPGIGPRMAAPDRLNHIWYQSFHQMEMAVRLVGASRESCRLYISHFLKGWAHRKDAFDD 215 Query: 181 VIEDFADNFCKPGNLAGGFAHYRASHAARVKMMQGEAPALPPITVPTCIRWAEHDPLFPY 240 V++ F DNF + GNLAGGFAHYRASHA RV MM+GEAP LPPI VPTC+RWAEHDPLFPY Sbjct: 216 VLDAFVDNFSRVGNLAGGFAHYRASHAGRVAMMKGEAPQLPPIDVPTCVRWAEHDPLFPY 275 Query: 241 AWTDRLGETFSDLDLGMFLDVGHFPHREDPDRAAAEIAGFFARINWK 287 AWTDRLGETFS LDL MF GHFPHRE+PD AAAEIA FF R+ W Sbjct: 276 AWTDRLGETFSALDLAMFPGAGHFPHRENPDHAAAEIAAFFQRVGWS 322 >ref|YP_001924150.1| alpha/beta hydrolase fold protein [Methylobacterium populi BJ001] gb|ACB79615.1| alpha/beta hydrolase fold [Methylobacterium populi BJ001] Length = 294 Score = 359 bits (921), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 175/277 (63%), Positives = 206/277 (74%), Gaps = 5/277 (1%) Query: 1 MEHVTIRANGAAFHVARTGAGRPLLLLHGWPEFWLTWKPVMARLADRYTLIAPDLRGFGD 60 + H T+ ANG HVAR GAGRPL+LLHGWPEFWLTW+PVMARLADR++LIAPDLRGFG Sbjct: 4 IRHETVTANGVQLHVARAGAGRPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 61 SDKPHGA----YGPDQHTDDMLALLDALGVDKAGVVGHDVGGAVMQPLARKAPDRIAGLF 116 S+KP GP+ H D++ LLDALG+++ GVV HDVG +V Q L R P+R+ GLF Sbjct: 64 SEKPDPGPSDRAGPEVHAADLVGLLDALGLERVGVVAHDVGASVGQALGRANPERLTGLF 123 Query: 117 LFDFVYPGIGPRMAAPDRLNHIWYQSFHQMEMAPALVGASRDSCRTYIRHFLHNWSH-RK 175 FD YPGIGPR+A P+ L IWYQSFH A +VGASR+SCR YI HFL +W+ Sbjct: 124 FFDCPYPGIGPRLARPEMLAEIWYQSFHLKPFAAGVVGASRESCRAYIGHFLSHWAGGNP 183 Query: 176 AAFDDVIEDFADNFCKPGNLAGGFAHYRASHAARVKMMQGEAPALPPITVPTCIRWAEHD 235 +AFDDV++ F DNF PGNL GGF Y + A R+ M++GEAPALPPI VPTCIRW D Sbjct: 184 SAFDDVLDAFTDNFLVPGNLQGGFNWYLSQQAERLAMVRGEAPALPPIGVPTCIRWGTRD 243 Query: 236 PLFPYAWTDRLGETFSDLDLGMFLDVGHFPHREDPDR 272 PLFPY WTDRLGETFSDLDL F + GHFPHRE PD+ Sbjct: 244 PLFPYEWTDRLGETFSDLDLAPFEETGHFPHRERPDQ 280 >ref|YP_001638925.1| alpha/beta hydrolase fold protein [Methylobacterium extorquens PA1] gb|ABY29854.1| alpha/beta hydrolase fold [Methylobacterium extorquens PA1] Length = 294 Score = 358 bits (919), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 182/290 (62%), Positives = 213/290 (73%), Gaps = 5/290 (1%) Query: 1 MEHVTIRANGAAFHVARTGAGRPLLLLHGWPEFWLTWKPVMARLADRYTLIAPDLRGFGD 60 + H T+ NG HVAR G G PL+LLHGWPEFWLTW+PVMARLADR++LIAPDLRGFG Sbjct: 4 IRHDTVTGNGMQLHVARAGTGSPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 61 SDKP-HG---AYGPDQHTDDMLALLDALGVDKAGVVGHDVGGAVMQPLARKAPDRIAGLF 116 S+KP HG GP+ H D++ LLDALG+ +AG+VGHDVG +V Q L R PDR+ GLF Sbjct: 64 SEKPDHGPSDKAGPEVHAADIVGLLDALGLARAGIVGHDVGASVGQALGRSHPDRLTGLF 123 Query: 117 LFDFVYPGIGPRMAAPDRLNHIWYQSFHQMEMAPALVGASRDSCRTYIRHFLHNWSH-RK 175 FD YPGIGPR+A P+ L IWYQSFH A +VGASR+SCR YI HFL +W+ Sbjct: 124 FFDCPYPGIGPRLAQPEMLAEIWYQSFHLKPFAAEVVGASRESCRAYIGHFLRHWAGGNP 183 Query: 176 AAFDDVIEDFADNFCKPGNLAGGFAHYRASHAARVKMMQGEAPALPPITVPTCIRWAEHD 235 +AFD V++ F DNF PGNL GGF Y + A R+ M++GEAPALPPI VPTCIRW D Sbjct: 184 SAFDAVLDAFTDNFLVPGNLQGGFNWYLSRQAGRLAMLRGEAPALPPIRVPTCIRWGTQD 243 Query: 236 PLFPYAWTDRLGETFSDLDLGMFLDVGHFPHREDPDRAAAEIAGFFARIN 285 PLFPYAWTDRLGETFS+LDL F GHFPHRE P+ AAA IA FF R+ Sbjct: 244 PLFPYAWTDRLGETFSNLDLAPFEGAGHFPHRERPEEAAAAIAAFFGRLE 293 >ref|YP_002962483.1| alpha/beta hydrolase, epoxide hydrolase [methylobacterium extorquens AM1] gb|ACS39206.1| putative alpha/beta hydrolase, putative epoxide hydrolase [Methylobacterium extorquens AM1] Length = 294 Score = 357 bits (916), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 181/290 (62%), Positives = 212/290 (73%), Gaps = 5/290 (1%) Query: 1 MEHVTIRANGAAFHVARTGAGRPLLLLHGWPEFWLTWKPVMARLADRYTLIAPDLRGFGD 60 + H T+ NG HVAR G G PL+LLHGWPEFWLTW+PVMARLADR++LIAPDLRGFG Sbjct: 4 IRHDTVTGNGVQLHVARAGTGSPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 61 SDKP-HG---AYGPDQHTDDMLALLDALGVDKAGVVGHDVGGAVMQPLARKAPDRIAGLF 116 S+KP HG GP+ H D++ LLDALG+ + G+VGHDVG +V Q L R PDR+ GLF Sbjct: 64 SEKPDHGPSDKAGPEVHAADIVGLLDALGLARVGIVGHDVGASVGQALGRSHPDRLTGLF 123 Query: 117 LFDFVYPGIGPRMAAPDRLNHIWYQSFHQMEMAPALVGASRDSCRTYIRHFLHNWSH-RK 175 FD YPGIGPR+A P+ L IWYQSFH A +VGASR+SCR YI HFL +W+ Sbjct: 124 FFDCPYPGIGPRLAQPEMLAEIWYQSFHLKPFAAEVVGASRESCRAYIGHFLRHWAGGNP 183 Query: 176 AAFDDVIEDFADNFCKPGNLAGGFAHYRASHAARVKMMQGEAPALPPITVPTCIRWAEHD 235 +AFD V++ F DNF PGNL GGF Y + A R+ M++GEAPALPPI VPTCIRW D Sbjct: 184 SAFDAVLDAFTDNFLVPGNLQGGFNWYLSRQAGRLAMVRGEAPALPPIRVPTCIRWGTQD 243 Query: 236 PLFPYAWTDRLGETFSDLDLGMFLDVGHFPHREDPDRAAAEIAGFFARIN 285 PLFPYAWTDRLGETFS+LDL F GHFPHRE P+ AAA IA FF R+ Sbjct: 244 PLFPYAWTDRLGETFSNLDLAPFEGAGHFPHRERPEEAAAAIAAFFGRLE 293 >ref|YP_003067666.1| alpha/beta hydrolase [Methylobacterium extorquens DM4] emb|CAX23715.1| putative alpha/beta hydrolase, putative epoxide hydrolase [Methylobacterium extorquens DM4] Length = 294 Score = 353 bits (905), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 180/290 (62%), Positives = 212/290 (73%), Gaps = 5/290 (1%) Query: 1 MEHVTIRANGAAFHVARTGAGRPLLLLHGWPEFWLTWKPVMARLADRYTLIAPDLRGFGD 60 + H T+ +NG HVAR G G PL+LLHGWPEFWLTW+PVMARLADR++LIAPDLRGFG Sbjct: 4 IRHDTVTSNGVQLHVARAGTGSPLVLLHGWPEFWLTWEPVMARLADRFSLIAPDLRGFGA 63 Query: 61 SDKP-HG---AYGPDQHTDDMLALLDALGVDKAGVVGHDVGGAVMQPLARKAPDRIAGLF 116 S+KP HG GP+ H D++ LLDALG+ + G+VGHDVG +V Q L R PDR+ GLF Sbjct: 64 SEKPDHGPSDKAGPEVHAADIVGLLDALGLARVGIVGHDVGASVGQALGRSHPDRLTGLF 123 Query: 117 LFDFVYPGIGPRMAAPDRLNHIWYQSFHQMEMAPALVGASRDSCRTYIRHFLHNWSH-RK 175 FD YPGIGPR+A P+ L IWYQSFH A +VGASR+SCR YI HFL +W+ Sbjct: 124 FFDCPYPGIGPRLAQPEMLAEIWYQSFHLKPFAAEVVGASRESCRAYIGHFLRHWAGGNP 183 Query: 176 AAFDDVIEDFADNFCKPGNLAGGFAHYRASHAARVKMMQGEAPALPPITVPTCIRWAEHD 235 +AFD V++ F DNF PGNL GGF Y + A R+ M++GEAPALPPI VPTCIR D Sbjct: 184 SAFDAVLDAFTDNFLVPGNLQGGFNWYLSRQAGRLAMVRGEAPALPPIRVPTCIRRGTQD 243 Query: 236 PLFPYAWTDRLGETFSDLDLGMFLDVGHFPHREDPDRAAAEIAGFFARIN 285 PLFPYAWTDRLGETFS+LDL F GHFPHRE P+ AAA IA FF R+ Sbjct: 244 PLFPYAWTDRLGETFSNLDLAPFEGAGHFPHRERPEEAAAAIAAFFGRLE 293