BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0143.0042.p (262 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767479.1| hypothetical protein bll0839 [Bradyrhizobium ja... 406 e-111 gb|EGP87521.1| putative enol-lactone hydrolase [Mycosphaerella g... 303 1e-80 ref|ZP_04747306.1| hypothetical protein MkanA1_05000 [Mycobacter... 268 6e-70 ref|ZP_03570334.1| hydrolase, alpha/beta fold family [Burkholder... 156 2e-36 ref|YP_003321247.1| alpha/beta hydrolase fold protein [Sphaeroba... 152 6e-35 >ref|NP_767479.1| hypothetical protein bll0839 [Bradyrhizobium japonicum USDA 110] dbj|BAC46104.1| bll0839 [Bradyrhizobium japonicum USDA 110] Length = 254 Score = 406 bits (1044), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/252 (76%), Positives = 224/252 (88%) Query: 1 MAKLNRNGVEIYYEVHGSGPPLILTHGYSSTSAMWQGQIEALSQRHKLILWDMRGHGQSD 60 M K++R+GV IYYEVHG GPPL+LTHGYSSTSAMW+ Q++AL++ HKL+LWDMRGHGQSD Sbjct: 1 MPKIDRDGVGIYYEVHGDGPPLLLTHGYSSTSAMWREQVDALAKDHKLVLWDMRGHGQSD 60 Query: 61 YPDDPDAYSEALTVGDIAALLDAAGADKAIVGGLSLGGYMSLAFHRAHPERVRALLIIDT 120 YPDDP AYSEALTVGD+AA+LDA G+ +A++GGLSLGGYMSLAF RA+P+RVRALLIIDT Sbjct: 61 YPDDPKAYSEALTVGDMAAILDAVGSKRAVIGGLSLGGYMSLAFTRAYPQRVRALLIIDT 120 Query: 121 GPGFKKDDARDVWNKRARETGDRFDREGLEVLRSLSAERASVTHRNARGLALAARGMLAQ 180 GPGFKKDDAR+ WN RA T DR +REGL+VL++ + ERAS +HRNA GLALAARGML Q Sbjct: 121 GPGFKKDDAREAWNARALATADRLEREGLDVLKAATRERASASHRNASGLALAARGMLTQ 180 Query: 181 RDARVIESLPGIQVPALVVVGADDTPFLAASDYMAAKIPGAQKAVIPNAGHAVNIDQPQA 240 RDA+VIE LP I VP+L+VVGADDTPFLAASDYMAAKIPGAQK VIP AGHAVNIDQP+A Sbjct: 181 RDAKVIELLPDITVPSLIVVGADDTPFLAASDYMAAKIPGAQKVVIPAAGHAVNIDQPKA 240 Query: 241 FIAAVLPFLDGL 252 F+ AV+PFL L Sbjct: 241 FVDAVVPFLKNL 252 >gb|EGP87521.1| putative enol-lactone hydrolase [Mycosphaerella graminicola IPO323] Length = 280 Score = 303 bits (777), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 146/220 (66%), Positives = 178/220 (80%), Gaps = 2/220 (0%) Query: 32 SAMWQGQIEALSQRHKLILWDMRGHGQSDYPDDPDAYSEALTVGDIAALLDAAGA--DKA 89 S+MW GQIE LS+R +LI+WDMRGHG+S YP DP AYSEA TV DI +LDA KA Sbjct: 51 SSMWGGQIEPLSKRFRLIVWDMRGHGKSGYPSDPSAYSEAHTVEDIGLILDANCTPDTKA 110 Query: 90 IVGGLSLGGYMSLAFHRAHPERVRALLIIDTGPGFKKDDARDVWNKRARETGDRFDREGL 149 IVGGLSLGGYMSLAF+R++P+RV ALLIIDTGPGFKKD AR+ WN+ A +T + +++GL Sbjct: 111 IVGGLSLGGYMSLAFYRSYPKRVCALLIIDTGPGFKKDSAREEWNRTAIKTAEELEKDGL 170 Query: 150 EVLRSLSAERASVTHRNARGLALAARGMLAQRDARVIESLPGIQVPALVVVGADDTPFLA 209 + L S ER+ +HRNA GLALAARGML QRD+ +IESL ++VP+LVVVGA+D PFL Sbjct: 171 KALHRQSPERSRASHRNAEGLALAARGMLTQRDSTIIESLVRVEVPSLVVVGAEDKPFLV 230 Query: 210 ASDYMAAKIPGAQKAVIPNAGHAVNIDQPQAFIAAVLPFL 249 AS+YMA KIPGA+K V+P AGHAVNIDQP+AF+ AV+PFL Sbjct: 231 ASEYMAKKIPGAKKVVVPGAGHAVNIDQPKAFMQAVVPFL 270 >ref|ZP_04747306.1| hypothetical protein MkanA1_05000 [Mycobacterium kansasii ATCC 12478] Length = 253 Score = 268 bits (685), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 136/255 (53%), Positives = 177/255 (69%), Gaps = 5/255 (1%) Query: 1 MAKLNRNGVEIYYEVHG---SGPPLILTHGYSSTSAMWQGQIEALSQRHKLILWDMRGHG 57 M +L R+GV + VH G P++LTHG+ +T+ MW+ ++ALS +I+WD RGHG Sbjct: 1 MNRLQRDGVTLAVRVHNPLARGVPVLLTHGFGATAGMWEPNVDALSVDRPVIVWDQRGHG 60 Query: 58 QSDYPDDPDAYSEALTVGDIAALLDAAGADKAIVGGLSLGGYMSLAFHRAHPERVRALLI 117 SD PDD DAYSE L+V D+AA+LDAAG D+A++GG+SLGGY+SLAFH HP+RV AL++ Sbjct: 61 SSDAPDDIDAYSEQLSVADMAAILDAAGTDRAVLGGMSLGGYLSLAFHLVHPQRVAALVL 120 Query: 118 IDTGPGFKKDDARDVWNKRARETGDRFDREGLEVLRSLSAERASVTHRNARGLALAARGM 177 +DTGPG++ D+AR WN + +R G +S E H + GL AARG+ Sbjct: 121 VDTGPGYRNDEARAKWNAWVERRARQLERGGAPA--DVSPELTQAVHEHPEGLPRAARGV 178 Query: 178 LAQRDARVIESLPGIQVPALVVVGADDTPFLAASDYMAAKIPGAQKAVIPNAGHAVNIDQ 237 +AQ+DARVI SL I VP LV+VGA DT +LAA+DYMA KIP A+K VI NA HA N+DQ Sbjct: 179 MAQKDARVITSLDSITVPTLVIVGAQDTDYLAAADYMARKIPTARKVVIENARHAANMDQ 238 Query: 238 PQAFIAAVLPFLDGL 252 P+AF AV FLD L Sbjct: 239 PEAFNIAVREFLDHL 253 >ref|ZP_03570334.1| hydrolase, alpha/beta fold family [Burkholderia multivorans CGD2M] ref|ZP_03576978.1| hydrolase, alpha/beta fold family [Burkholderia multivorans CGD2] gb|EEE08555.1| hydrolase, alpha/beta fold family [Burkholderia multivorans CGD2] gb|EEE16241.1| hydrolase, alpha/beta fold family [Burkholderia multivorans CGD2M] Length = 274 Score = 156 bits (395), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 97/273 (35%), Positives = 143/273 (52%), Gaps = 23/273 (8%) Query: 1 MAKLNRNGVEIYYEVHGSGPPLILTHGYSSTSAMWQGQIEALSQRHKLILWDMRGHGQSD 60 M + +GV+++YEV G GP ++ H ++ W Q+ +LSQR + I ++ RG+ SD Sbjct: 1 MPFASSDGVKLFYEVTGQGPAIVFLHEFAGDYRNWTSQVRSLSQRFRCITFNARGYPPSD 60 Query: 61 YPDDPDAYSEALTVGDIAALLDAAGADKAIVGGLSLGGYMSLAFHRAHPERVRALLIIDT 120 PDD ++YS V DIA++L AAG A + GLS+GGY +L F AHPE R+++ Sbjct: 61 VPDDIESYSHVHAVEDIASVLRAAGVSNAHIVGLSMGGYAALCFAIAHPEMTRSIVAAAV 120 Query: 121 GPGFKKDDARDVWNKRARETGDRFDREGLEV---------LRSLSAER---------ASV 162 G G DA + + + R EGLEV +R E+ Sbjct: 121 GHG---SDAPEQFRAASEALSARILTEGLEVGAADYLNGPIRRKLEEKDPRGFDEFIRQF 177 Query: 163 THRNARGLALAARGMLAQRDA--RVIESLPGIQVPALVVVGADDTPFLAASDYMAAKIPG 220 +A G AL ARG +R + L ++ PALVV G DD P L S ++ IP Sbjct: 178 AEHSAIGTALTARGCQMRRPTLYELEAPLRELKAPALVVAGDDDEPCLGPSLFLKRTIPN 237 Query: 221 AQKAVIPNAGHAVNIDQPQAFIAAVLPFLDGLE 253 A+ V+PN HA+N+++P AF AVL F++ +E Sbjct: 238 ARLWVLPNTSHAINLEEPGAFNCAVLDFINDVE 270 >ref|YP_003321247.1| alpha/beta hydrolase fold protein [Sphaerobacter thermophilus DSM 20745] gb|ACZ40425.1| alpha/beta hydrolase fold protein [Sphaerobacter thermophilus DSM 20745] Length = 277 Score = 152 bits (383), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 100/267 (37%), Positives = 138/267 (51%), Gaps = 18/267 (6%) Query: 1 MAKLNRNGVEIYYEVHGSGPPLILTHGYSSTSAMWQGQIEALSQRHKLILWDMRGHGQSD 60 M + NGV ++ E G GPPL+L HG++ ++A W+ + AL + D+ GHG+SD Sbjct: 1 MGLIAVNGVRLHVERGGDGPPLLLLHGFTGSAATWEPLLPALQAHFHTVAPDLIGHGRSD 60 Query: 61 YPDDPDAYSEALTVGDIAALLDAAGADKAIVGGLSLGGYMSLAFHRAHPERVRALLIIDT 120 P DP Y+ V D+ ALLDA D+A V G S+GG +L A PERV AL++ Sbjct: 61 VPADPARYAMDRCVADLVALLDALDIDRAAVLGYSMGGRTALHLALAAPERVSALVLEGA 120 Query: 121 GPGFKKDDARDVWNKRARETGDRFDREGLEVL--------------RSLSAERASVTHR- 165 PG D R DR +REGL+ R A RA + + Sbjct: 121 SPGIADPDERAARVASDAALADRIEREGLQAFVDAWERLPLFASQQRLPEATRARLRAQR 180 Query: 166 ---NARGLALAARGMLAQRDARVIESLPGIQVPALVVVGADDTPFLAASDYMAAKIPGAQ 222 RGLA + RGM A A V + L I VP L++ G D ++ S MA +P A Sbjct: 181 LSHTPRGLANSLRGMGAGAMAPVHDRLGEIAVPVLLIAGELDGKYVEISRAMAGAMPQAT 240 Query: 223 KAVIPNAGHAVNIDQPQAFIAAVLPFL 249 +IP+AGHA +++QP+AF+ AVL FL Sbjct: 241 VRIIPDAGHAPHLEQPEAFVEAVLGFL 267