BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0140.0041.p (337 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_773295.1| thiamine biosynthesis oxidoreductase [Bradyrhiz... 536 e-150 ref|YP_578119.1| glycine oxidase ThiO [Nitrobacter hamburgensis ... 519 e-145 ref|ZP_08630988.1| glycine oxidase ThiO [Bradyrhizobiaceae bacte... 516 e-144 ref|ZP_01046955.1| FAD dependent oxidoreductase [Nitrobacter sp.... 510 e-142 ref|YP_319069.1| FAD dependent oxidoreductase [Nitrobacter winog... 509 e-142 >ref|NP_773295.1| thiamine biosynthesis oxidoreductase [Bradyrhizobium japonicum USDA 110] dbj|BAC51920.1| thiamine biosynthesis oxidoreductase [Bradyrhizobium japonicum USDA 110] Length = 336 Score = 536 bits (1381), Expect = e-150, Method: Compositional matrix adjust. Identities = 263/336 (78%), Positives = 291/336 (86%), Gaps = 1/336 (0%) Query: 1 MYQTSRLRGDSPVTIIGAGIAGAWHALLLAEAGRAVTLHERSDAAMTESTSYFAGGMLAP 60 M QT + R DS V+IIGAGIAGAW ALL A+AG AVTLHER DAAMT+STS++AGGMLAP Sbjct: 1 MLQTIK-RADSAVSIIGAGIAGAWQALLFAQAGHAVTLHERGDAAMTDSTSHWAGGMLAP 59 Query: 61 WCEAEASEPVISRLGIRSLELWRQHVPETPFNGSLVVAHPRDRADFERFARLTSGHRRLD 120 WCEAE +EP+ISRLG RSL++WR+ +P+TPFNGSLVVAHPR+R DFERFAR+T HRRLD Sbjct: 60 WCEAEVAEPIISRLGQRSLDIWRRELPDTPFNGSLVVAHPRERNDFERFARMTEEHRRLD 119 Query: 121 AEGVRALEPSLEGRFRDALFYPDEGHVEPRRVLPRLHAAIAKAGGAIKFGSDAEATDLDG 180 A + LEPSLEGRFRDALF+P EGHVEPRRVLP+LH I AGG IKF SD A DLDG Sbjct: 120 AASLAELEPSLEGRFRDALFFPTEGHVEPRRVLPKLHERIRAAGGTIKFSSDVSAADLDG 179 Query: 181 LVIDCRGFAAHDREPSLRGVKGEMIIIETSEVELARPVRLIHPRWPLYVIPRGDGRFMLG 240 +VIDCRG +A D +P LRGVKGEMI+IETSEV+LARPVRLIHPRWPLYVIPR D FMLG Sbjct: 180 IVIDCRGLSARDEQPDLRGVKGEMILIETSEVQLARPVRLIHPRWPLYVIPREDNLFMLG 239 Query: 241 ATSIEAEDNGVSVRSALELLGAAYVVHPAFAEARIVEFGAGLRPAFPDNLPKIRIEQERI 300 ATSIEAED GVSVRSALELLGAAY VHPAF EARIVEFG+GLRPAFPDNLP+I I RI Sbjct: 240 ATSIEAEDTGVSVRSALELLGAAYTVHPAFGEARIVEFGSGLRPAFPDNLPRIGIRGGRI 299 Query: 301 TVNGLYRHGFLLAPALAELTLAYLARGEIDNEVMQC 336 TVNGLYRHGFL+APALAELTL Y+ RG+IDNEVMQC Sbjct: 300 TVNGLYRHGFLIAPALAELTLQYVQRGQIDNEVMQC 335 >ref|YP_578119.1| glycine oxidase ThiO [Nitrobacter hamburgensis X14] gb|ABE63659.1| glycine oxidase [Nitrobacter hamburgensis X14] Length = 338 Score = 519 bits (1337), Expect = e-145, Method: Compositional matrix adjust. Identities = 250/337 (74%), Positives = 291/337 (86%), Gaps = 1/337 (0%) Query: 1 MYQTSR-LRGDSPVTIIGAGIAGAWHALLLAEAGRAVTLHERSDAAMTESTSYFAGGMLA 59 M+Q +R + GDSPV +IGAGIAGAW ALL A+AG AVTLHER AMT++TS++AGGMLA Sbjct: 1 MFQNTREIWGDSPVAVIGAGIAGAWQALLFAKAGYAVTLHERGSDAMTQATSHWAGGMLA 60 Query: 60 PWCEAEASEPVISRLGIRSLELWRQHVPETPFNGSLVVAHPRDRADFERFARLTSGHRRL 119 PWCE+E SEP+++RLG+RSL LWR+HVP+TPFNGSLVVAHPRDR+DFERFAR+TSGH+R+ Sbjct: 61 PWCESEVSEPLVTRLGLRSLALWREHVPDTPFNGSLVVAHPRDRSDFERFARMTSGHQRI 120 Query: 120 DAEGVRALEPSLEGRFRDALFYPDEGHVEPRRVLPRLHAAIAKAGGAIKFGSDAEATDLD 179 + V LEPSL+GRFR+ALF+P EGHVEPRRVLP+LHA I AGG I+F S +A LD Sbjct: 121 QGDKVSELEPSLDGRFREALFFPHEGHVEPRRVLPQLHARIRAAGGKIEFDSAPDARHLD 180 Query: 180 GLVIDCRGFAAHDREPSLRGVKGEMIIIETSEVELARPVRLIHPRWPLYVIPRGDGRFML 239 G+ IDCRG AA D P LRGVKGE II+ET E+ L+RPVRL+HPRWPLY+IPR D RFM+ Sbjct: 181 GMAIDCRGLAARDTFPDLRGVKGEEIIVETDEIALSRPVRLLHPRWPLYIIPREDNRFMI 240 Query: 240 GATSIEAEDNGVSVRSALELLGAAYVVHPAFAEARIVEFGAGLRPAFPDNLPKIRIEQER 299 GATSIE ED+GVSVRSALELL AAY VHPAF EARIVE GAGLRPAFPDNLP+I I++ R Sbjct: 241 GATSIEREDDGVSVRSALELLSAAYAVHPAFGEARIVEIGAGLRPAFPDNLPRIAIDKNR 300 Query: 300 ITVNGLYRHGFLLAPALAELTLAYLARGEIDNEVMQC 336 I VNGL+RHGFLL+PALAELTLAY+ARGEIDNEVMQC Sbjct: 301 IAVNGLFRHGFLLSPALAELTLAYVARGEIDNEVMQC 337 >ref|ZP_08630988.1| glycine oxidase ThiO [Bradyrhizobiaceae bacterium SG-6C] gb|EGP06288.1| glycine oxidase ThiO [Bradyrhizobiaceae bacterium SG-6C] Length = 340 Score = 516 bits (1328), Expect = e-144, Method: Compositional matrix adjust. Identities = 252/340 (74%), Positives = 292/340 (85%), Gaps = 3/340 (0%) Query: 1 MYQ--TSRLRGDSPVTIIGAGIAGAWHALLLAEAGRAVTLHERSDAAMTESTSYFAGGML 58 MY+ +S RGDS V IIGAGIAG WHALL A AG VTLHER DAAMT++TS++AGGML Sbjct: 1 MYRDTSSSPRGDSSVNIIGAGIAGTWHALLFARAGYDVTLHERDDAAMTQATSHWAGGML 60 Query: 59 APWCEAEASEPVISRLGIRSLELWRQHVPETPFNGSLVVAHPRDRADFERFARLTSGHRR 118 APWCE EASEPVI+RLGIRSL+LWR+ VPETPFNGSLVVAHPRDR DFERFARLT+ + R Sbjct: 61 APWCEREASEPVITRLGIRSLDLWREQVPETPFNGSLVVAHPRDRTDFERFARLTTDYER 120 Query: 119 LDAEGVRALEPSLEGRFRDALFYPDEGHVEPRRVLPRLHAAIAKAGGAIKFGSDAEATDL 178 LDA G+ +EPSL+GRFR+AL +P EGHVEPR VLP+LHA +A+AG I FGSD + D+ Sbjct: 121 LDASGIANVEPSLDGRFREALLFPGEGHVEPRAVLPKLHAKLAEAGATISFGSDRKPEDI 180 Query: 179 -DGLVIDCRGFAAHDREPSLRGVKGEMIIIETSEVELARPVRLIHPRWPLYVIPRGDGRF 237 DG+VIDCRG +A DR P LRGVKGE++I+ET E++L+RPVRL+HPRWPLY+IPR RF Sbjct: 181 ADGIVIDCRGLSARDRFPELRGVKGELVIVETPEIQLSRPVRLLHPRWPLYIIPRDGNRF 240 Query: 238 MLGATSIEAEDNGVSVRSALELLGAAYVVHPAFAEARIVEFGAGLRPAFPDNLPKIRIEQ 297 M+GATSIE+ED GVSVRSALELL AAY VHPAFAEARI+E GAGLRPAFPDNLP+I I+ Sbjct: 241 MIGATSIESEDTGVSVRSALELLTAAYAVHPAFAEARILEIGAGLRPAFPDNLPRIAIDA 300 Query: 298 ERITVNGLYRHGFLLAPALAELTLAYLARGEIDNEVMQCA 337 +RI+VNGLYRHGFLLAPALAELTLAY+ RG IDNEVMQ + Sbjct: 301 KRISVNGLYRHGFLLAPALAELTLAYVERGAIDNEVMQWS 340 >ref|ZP_01046955.1| FAD dependent oxidoreductase [Nitrobacter sp. Nb-311A] gb|EAQ35057.1| FAD dependent oxidoreductase [Nitrobacter sp. Nb-311A] Length = 338 Score = 510 bits (1313), Expect = e-142, Method: Compositional matrix adjust. Identities = 247/337 (73%), Positives = 284/337 (84%), Gaps = 1/337 (0%) Query: 1 MYQTSR-LRGDSPVTIIGAGIAGAWHALLLAEAGRAVTLHERSDAAMTESTSYFAGGMLA 59 M Q +R R +P+ +IGAGIAGAW ALL A+AG AVTLHER + AM +TSY+AGGMLA Sbjct: 1 MLQKAREKRATTPIAVIGAGIAGAWQALLFAKAGHAVTLHERGNDAMASATSYWAGGMLA 60 Query: 60 PWCEAEASEPVISRLGIRSLELWRQHVPETPFNGSLVVAHPRDRADFERFARLTSGHRRL 119 PWCEAE SEP+++RLG+RSL LWR+H P+TPFNGSLVVAHPRDR+DFERFA +TSGH+RL Sbjct: 61 PWCEAEVSEPLVTRLGLRSLALWREHAPDTPFNGSLVVAHPRDRSDFERFAHMTSGHQRL 120 Query: 120 DAEGVRALEPSLEGRFRDALFYPDEGHVEPRRVLPRLHAAIAKAGGAIKFGSDAEATDLD 179 + V LEPSL+GRFR+ALF+PDEGHVEPRRVLP LHA I AGG I+FGS EA+ ++ Sbjct: 121 QCDEVSKLEPSLDGRFREALFFPDEGHVEPRRVLPELHACIRAAGGQIQFGSAPEASGIE 180 Query: 180 GLVIDCRGFAAHDREPSLRGVKGEMIIIETSEVELARPVRLIHPRWPLYVIPRGDGRFML 239 L IDCRG AA D P LRGVKGE II+ET E+ L+RPVRL+HPRWPLY+IPR RFM+ Sbjct: 181 ALAIDCRGLAARDTFPDLRGVKGEEIIVETDEIVLSRPVRLLHPRWPLYIIPREGNRFMI 240 Query: 240 GATSIEAEDNGVSVRSALELLGAAYVVHPAFAEARIVEFGAGLRPAFPDNLPKIRIEQER 299 GATSIE ED+GVSVRSALELL AAY VHPAF EARIVE G+GLRPAFPDNLP+I I +ER Sbjct: 241 GATSIEREDDGVSVRSALELLSAAYAVHPAFGEARIVEIGSGLRPAFPDNLPRIAIGKER 300 Query: 300 ITVNGLYRHGFLLAPALAELTLAYLARGEIDNEVMQC 336 I VNGL+RHGFLLAPALAELTL Y+ARGEIDNEVMQC Sbjct: 301 IAVNGLFRHGFLLAPALAELTLGYVARGEIDNEVMQC 337 >ref|YP_319069.1| FAD dependent oxidoreductase [Nitrobacter winogradskyi Nb-255] gb|ABA05717.1| glycine oxidase [Nitrobacter winogradskyi Nb-255] Length = 338 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 244/336 (72%), Positives = 285/336 (84%) Query: 1 MYQTSRLRGDSPVTIIGAGIAGAWHALLLAEAGRAVTLHERSDAAMTESTSYFAGGMLAP 60 +++T RG P+ +IGAGIAGAW ALL A+AG VTLHER + AMT++TS++AGGMLAP Sbjct: 2 LHKTWDRRGTPPIAVIGAGIAGAWQALLFAKAGHEVTLHERDNDAMTQATSHWAGGMLAP 61 Query: 61 WCEAEASEPVISRLGIRSLELWRQHVPETPFNGSLVVAHPRDRADFERFARLTSGHRRLD 120 WCE+E SEP+++RLG+RSL LWR+H P+TPFNGSLVVAHPRDR+DFERFAR+TSGH+RL Sbjct: 62 WCESEVSEPLVTRLGLRSLALWREHAPDTPFNGSLVVAHPRDRSDFERFARMTSGHQRLQ 121 Query: 121 AEGVRALEPSLEGRFRDALFYPDEGHVEPRRVLPRLHAAIAKAGGAIKFGSDAEATDLDG 180 + V LEPSL+GRFR+ALF+PDEGHVEPRRVLP LHA I AGG I+FGS E T +DG Sbjct: 122 RDDVSKLEPSLDGRFREALFFPDEGHVEPRRVLPELHARIRAAGGRIEFGSAPETTHIDG 181 Query: 181 LVIDCRGFAAHDREPSLRGVKGEMIIIETSEVELARPVRLIHPRWPLYVIPRGDGRFMLG 240 L IDCRG AA D P LRGVKGE II+ET E+ L+RP+R++HPRWPLY+IPR RFM+G Sbjct: 182 LEIDCRGLAARDAFPDLRGVKGEEIIVETDEIVLSRPIRILHPRWPLYIIPRERNRFMIG 241 Query: 241 ATSIEAEDNGVSVRSALELLGAAYVVHPAFAEARIVEFGAGLRPAFPDNLPKIRIEQERI 300 ATSIE ED+GVSVRS LELL AAY VHPAF EARIVE GAGLRPAFPDNLP+I I+++ I Sbjct: 242 ATSIEREDDGVSVRSTLELLSAAYAVHPAFGEARIVEIGAGLRPAFPDNLPRIAIDKDHI 301 Query: 301 TVNGLYRHGFLLAPALAELTLAYLARGEIDNEVMQC 336 VNGLYRHGFLLAPALAELTL Y+ARGEIDNEVMQC Sbjct: 302 AVNGLYRHGFLLAPALAELTLGYVARGEIDNEVMQC 337