BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0140.0026.p (415 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_773410.1| alpha-amylase [Bradyrhizobium japonicum USDA 11... 625 e-177 ref|YP_001207700.1| putative maltooligosyltrehalose trehalohydro... 611 e-173 ref|YP_001242149.1| putative maltooligosyltrehalose trehalohydro... 603 e-170 ref|YP_485499.1| malto-oligosyltrehalose trehalohydrolase [Rhodo... 599 e-169 ref|YP_004108055.1| malto-oligosyltrehalose trehalohydrolase [Rh... 591 e-167 >ref|NP_773410.1| alpha-amylase [Bradyrhizobium japonicum USDA 110] dbj|BAC52035.1| alpha-amylase [Bradyrhizobium japonicum USDA 110] Length = 583 Score = 625 bits (1612), Expect = e-177, Method: Compositional matrix adjust. Identities = 299/407 (73%), Positives = 339/407 (83%), Gaps = 1/407 (0%) Query: 3 RQHFGAELTPDGTRFRLWAPAAKRVGLLIDQPHRLTRDAAGWYVAEIPGARAGTRYKFRV 62 R FGA T +G FRLWAP A+RV LL+D+ H + R GWYVAEI G+ YKFR+ Sbjct: 2 RPSFGARPTKEGVSFRLWAPGARRVDLLLDRRHAMQRRQDGWYVAEIASLTTGSPYKFRI 61 Query: 63 DDEVDVPDPASAFQPDDVFGPSEVIDHAAFKWRATEWRGRPWHEAVVLEAHVGTFTPEGT 122 DDE+DVPDPASAFQP+DVFGPSEVIDH AF WRA +WRGRPW E V++E+HVGTFT EGT Sbjct: 62 DDEIDVPDPASAFQPEDVFGPSEVIDHDAFAWRARDWRGRPWEETVLIESHVGTFTAEGT 121 Query: 123 YRAMIDKLDHLVATGITALELMPLADFAGKRNWGYDGVLWYAPDSAYGRPDDLKTLIDEA 182 YRAMID+LDHL ATGITALELMPLADFAG R WGYDGVLWYAPDSAYGRP+DLKTLIDEA Sbjct: 122 YRAMIDRLDHLTATGITALELMPLADFAGYRGWGYDGVLWYAPDSAYGRPEDLKTLIDEA 181 Query: 183 HLRGLMVMLDVVYNHFGPEGNYLGRYAPSFFSDAHTPWGSAIDYRVPEVRAFAIGSVVSW 242 HLRGLMV LDVVYNHFGPEGNYLGRYAPSFF+DAHTPWGSAIDYR+ +VRAFA+ + +SW Sbjct: 182 HLRGLMVFLDVVYNHFGPEGNYLGRYAPSFFTDAHTPWGSAIDYRLSQVRAFAVENALSW 241 Query: 243 LRDYRFDGLRFDAVNTIVE-AGEISVLHDFSRAAGKLATETGRDINLVLENGDNIASLLD 301 L DYRFDGLR DA N I+ GE S+L D S AAG+LA TGR I+LVLENGDN ASLLD Sbjct: 242 LSDYRFDGLRLDAANHIMALPGEQSMLQDLSVAAGELAKATGRHIHLVLENGDNRASLLD 301 Query: 302 ATEDPPHGKFRGQWNDDYHHAWHVLLTGETQGYYGDYRAPKQDLARALSSGYIYQGEISD 361 A ++PP+GK+R QWNDDYHH WHV+LTGE GYY DY+ P+ DLARAL+SG++YQGEIS+ Sbjct: 302 AAQEPPNGKYRAQWNDDYHHVWHVMLTGELAGYYADYQTPRMDLARALASGFVYQGEISE 361 Query: 362 FWGGKARGEPSGALSPTCFINFLQNHDQIGNRPLGDRLLSIARPEAV 408 FWG K RGEPS L P F+NFLQNHDQIGNRPLG+RL S+A P + Sbjct: 362 FWGKKPRGEPSRELPPATFVNFLQNHDQIGNRPLGERLESLASPRQI 408 >ref|YP_001207700.1| putative maltooligosyltrehalose trehalohydrolase [Bradyrhizobium sp. ORS 278] emb|CAL79483.1| putative maltooligosyltrehalose trehalohydrolase [Bradyrhizobium sp. ORS 278] Length = 588 Score = 611 bits (1576), Expect = e-173, Method: Compositional matrix adjust. Identities = 295/410 (71%), Positives = 334/410 (81%), Gaps = 2/410 (0%) Query: 1 MNRQHFGAELTPDGTRFRLWAPAAKRVGLLIDQPHRLTRDAAGWYVAEIPGARAGTRYKF 60 M + FG +T +GT FRLWAPAAKRV L++D H + R GWY + G AG RYKF Sbjct: 1 MTARQFGPAITANGTTFRLWAPAAKRVDLVLDGQHEMQRGDGGWYTLHVSGVGAGARYKF 60 Query: 61 RVDDEVDVPDPASAFQPDDVFGPSEVIDHAAFKWRATEWRGRPWHEAVVLEAHVGTFTPE 120 R++DE+DVPDP S FQP+DVFGPSEVIDH + WRA +WRGRPW +AV LE+HVGTFT Sbjct: 61 RINDELDVPDPGSDFQPEDVFGPSEVIDHGRYAWRAEDWRGRPWQDAVFLESHVGTFTQH 120 Query: 121 GTYRAMIDKLDHLVATGITALELMPLADFAGKRNWGYDGVLWYAPDSAYGRPDDLKTLID 180 GTYRAMIDKLDHLV TGITALELMPLADFAG RNWGYDGVL YAPDS YGRPDDL+ LID Sbjct: 121 GTYRAMIDKLDHLVETGITALELMPLADFAGSRNWGYDGVLLYAPDSVYGRPDDLRALID 180 Query: 181 EAHLRGLMVMLDVVYNHFGPEGNYLGRYAPSFFSDAHTPWGSAIDYRVPEVRAFAIGSVV 240 EAHLRGLMV LDVVYNHFGPEGN++G YAP+FF+DAHTPWGSAIDYR PEVRAFAI + + Sbjct: 181 EAHLRGLMVFLDVVYNHFGPEGNFIGVYAPTFFTDAHTPWGSAIDYRRPEVRAFAIENAL 240 Query: 241 SWLRDYRFDGLRFDAVNTIV-EAGEISVLHDFSRAAGKLATETGRDINLVLENGDNIASL 299 WL DYRFDGLRFDAVN I+ GEI +LHD S A G+L TGR I+LVLENGDN AS+ Sbjct: 241 HWLTDYRFDGLRFDAVNHILHNEGEIPMLHDLSAAVGRLVERTGRQIHLVLENGDNRASM 300 Query: 300 LDATEDPPHGKFRGQWNDDYHHAWHVLLTGETQGYYGDY-RAPKQDLARALSSGYIYQGE 358 LDA EDPP GKFR QWNDDYHHAWHV+LTGET GYYGDY ++P +DLARAL+SG++YQGE Sbjct: 301 LDAAEDPPRGKFRAQWNDDYHHAWHVMLTGETGGYYGDYQQSPTRDLARALASGFVYQGE 360 Query: 359 ISDFWGGKARGEPSGALSPTCFINFLQNHDQIGNRPLGDRLLSIARPEAV 408 + FWGG RGEPSG L+P F+NFLQNHDQIGNR GDRL +A PE + Sbjct: 361 QATFWGGINRGEPSGHLAPGAFVNFLQNHDQIGNRVFGDRLEVLAPPEGI 410 >ref|YP_001242149.1| putative maltooligosyltrehalose trehalohydrolase [Bradyrhizobium sp. BTAi1] gb|ABQ38243.1| maltooligosyl trehalose hydrolase [Bradyrhizobium sp. BTAi1] Length = 589 Score = 603 bits (1555), Expect = e-170, Method: Compositional matrix adjust. Identities = 294/411 (71%), Positives = 336/411 (81%), Gaps = 3/411 (0%) Query: 1 MNRQHFGAELTPDGTRFRLWAPAAKRVGLLIDQPHR-LTRDAAGWYVAEIPGARAGTRYK 59 M+ + FG L+ GT FRLWAPAA+RV L++D R + R GWY + PG AGTRYK Sbjct: 1 MSGRQFGPRLSEHGTSFRLWAPAARRVDLVLDDGRREMQRRDGGWYALDAPGIGAGTRYK 60 Query: 60 FRVDDEVDVPDPASAFQPDDVFGPSEVIDHAAFKWRATEWRGRPWHEAVVLEAHVGTFTP 119 FR+DDE+DVPDP S FQP+DV GPSEVIDH A+ WRA +WRGRPW +AV LE+HVGTFT Sbjct: 61 FRIDDELDVPDPGSDFQPEDVSGPSEVIDHCAYVWRAKDWRGRPWQDAVFLESHVGTFTE 120 Query: 120 EGTYRAMIDKLDHLVATGITALELMPLADFAGKRNWGYDGVLWYAPDSAYGRPDDLKTLI 179 EGTYRAMIDKLDHLV TGITALELMPLADFAG+RNWGYDGVL YAPDS YGRPDDL+ LI Sbjct: 121 EGTYRAMIDKLDHLVETGITALELMPLADFAGRRNWGYDGVLLYAPDSVYGRPDDLRALI 180 Query: 180 DEAHLRGLMVMLDVVYNHFGPEGNYLGRYAPSFFSDAHTPWGSAIDYRVPEVRAFAIGSV 239 DEAHLRGLMV LDVVYNHFGPEGNY+ RYAP+FF+D HTPWGSAIDYR PEVRAFAI + Sbjct: 181 DEAHLRGLMVFLDVVYNHFGPEGNYIARYAPTFFTDTHTPWGSAIDYRRPEVRAFAIENA 240 Query: 240 VSWLRDYRFDGLRFDAVNTIV-EAGEISVLHDFSRAAGKLATETGRDINLVLENGDNIAS 298 + WL DYRFDGLRFDAVN I+ GEI +LH+ S A G+L +TGR I+LVLENGDN AS Sbjct: 241 LHWLTDYRFDGLRFDAVNHILSNEGEIPMLHELSAAVGRLVAQTGRHIHLVLENGDNRAS 300 Query: 299 LLDATEDPPHGKFRGQWNDDYHHAWHVLLTGETQGYYGDYR-APKQDLARALSSGYIYQG 357 +LDA E+PP GKFR QWNDDYHH WHV+LTGET GYYGDY+ P+ LARAL+SG++YQG Sbjct: 301 MLDAAEEPPRGKFRAQWNDDYHHVWHVMLTGETTGYYGDYQDNPRGGLARALASGFVYQG 360 Query: 358 EISDFWGGKARGEPSGALSPTCFINFLQNHDQIGNRPLGDRLLSIARPEAV 408 E + FWGG RGEPSG L+P F+NFLQNHDQIGNR GDRL ++A PE + Sbjct: 361 EQAAFWGGIRRGEPSGHLAPGAFVNFLQNHDQIGNRVFGDRLEALAPPEGI 411 >ref|YP_485499.1| malto-oligosyltrehalose trehalohydrolase [Rhodopseudomonas palustris HaA2] gb|ABD06588.1| Malto-oligosyltrehalose trehalohydrolase [Rhodopseudomonas palustris HaA2] Length = 587 Score = 599 bits (1545), Expect = e-169, Method: Compositional matrix adjust. Identities = 286/409 (69%), Positives = 333/409 (81%), Gaps = 1/409 (0%) Query: 1 MNRQHFGAELTPDGTRFRLWAPAAKRVGLLIDQPHRLTRDAAGWYVAEIPGARAGTRYKF 60 M + FG LT +G FRLWAP A RV +++D+PH + RD GW+ EI GAR GTRY+F Sbjct: 1 MKGRSFGPRLTENGAEFRLWAPNATRVDVVLDRPHAMQRDKDGWFKVEIDGARGGTRYRF 60 Query: 61 RVDDEVDVPDPASAFQPDDVFGPSEVIDHAAFKWRATEWRGRPWHEAVVLEAHVGTFTPE 120 R+DD DVPDPAS FQP+D+ GPSEVIDHAA+ WRA EWRGRPWHE V+LEAHVG+FTP+ Sbjct: 61 RIDDTTDVPDPASGFQPEDIQGPSEVIDHAAYPWRAGEWRGRPWHETVLLEAHVGSFTPQ 120 Query: 121 GTYRAMIDKLDHLVATGITALELMPLADFAGKRNWGYDGVLWYAPDSAYGRPDDLKTLID 180 GT+RAMID+LDHLV TG+TALELMPLADF G+RNWGYDGVLWYAPDSAYGRP+DLK LID Sbjct: 121 GTFRAMIDRLDHLVDTGVTALELMPLADFPGRRNWGYDGVLWYAPDSAYGRPEDLKALID 180 Query: 181 EAHLRGLMVMLDVVYNHFGPEGNYLGRYAPSFFSDAHTPWGSAIDYRVPEVRAFAIGSVV 240 AH RGLM+ LDVVYNHFGPEGNY+G+YAP FFSD+HTPWG+ I+Y V +VRAFAI + V Sbjct: 181 AAHERGLMMFLDVVYNHFGPEGNYIGQYAPPFFSDSHTPWGNGINYDVEQVRAFAIENAV 240 Query: 241 SWLRDYRFDGLRFDAVNTIVEAGEISVLHDFSRAAGKLATETGRDINLVLENGDNIASLL 300 WLR+Y FDGLR DAV+ I + GEI +LH+ SR GKLA ETGR I+LVLEN DNIA++L Sbjct: 241 YWLREYHFDGLRLDAVHAIPDQGEIPMLHELSREVGKLAAETGRHIHLVLENDDNIAAVL 300 Query: 301 DATEDPPHGKFRGQWNDDYHHAWHVLLTGETQGYYGDYR-APKQDLARALSSGYIYQGEI 359 D DPP G++R QWNDDYHHAWHV LTGE QGYY DY AP +ARAL SG++YQGE Sbjct: 301 DPVVDPPRGQYRAQWNDDYHHAWHVALTGEKQGYYSDYADAPLNAIARALGSGFVYQGEP 360 Query: 360 SDFWGGKARGEPSGALSPTCFINFLQNHDQIGNRPLGDRLLSIARPEAV 408 S GG+ RGEPSG L P F+NFLQNHDQIGNR LGDRL S+A+P+AV Sbjct: 361 SGHRGGQPRGEPSGKLPPLAFVNFLQNHDQIGNRALGDRLESLAKPKAV 409 >ref|YP_004108055.1| malto-oligosyltrehalose trehalohydrolase [Rhodopseudomonas palustris DX-1] gb|ADU43322.1| malto-oligosyltrehalose trehalohydrolase [Rhodopseudomonas palustris DX-1] Length = 589 Score = 591 bits (1524), Expect = e-167, Method: Compositional matrix adjust. Identities = 290/410 (70%), Positives = 330/410 (80%), Gaps = 2/410 (0%) Query: 1 MNRQHFGAELTPDGTRFRLWAPAAKRVGLLID-QPHRLTRDAAGWYVAEIPGARAGTRYK 59 M + FG LT G FRLWAP A++V +++D H + RD GW+ AE+ G G RY+ Sbjct: 1 MTGRSFGPRLTAGGVEFRLWAPNAQQVEVVLDGATHPMLRDDKGWHAAEVAGPGVGARYR 60 Query: 60 FRVDDEVDVPDPASAFQPDDVFGPSEVIDHAAFKWRATEWRGRPWHEAVVLEAHVGTFTP 119 FR+D E++VPDP S FQPDD+ GPSEVIDHAA+ WRAT+WRGRPW + V+LE HVGTFTP Sbjct: 61 FRIDGELEVPDPGSLFQPDDISGPSEVIDHAAYAWRATDWRGRPWADTVLLETHVGTFTP 120 Query: 120 EGTYRAMIDKLDHLVATGITALELMPLADFAGKRNWGYDGVLWYAPDSAYGRPDDLKTLI 179 EG+YRAMIDKLDHLVATGITALELMPLADF G+RNWGYDGVLWYAPDSAYGRP+DLK LI Sbjct: 121 EGSYRAMIDKLDHLVATGITALELMPLADFPGQRNWGYDGVLWYAPDSAYGRPEDLKALI 180 Query: 180 DEAHLRGLMVMLDVVYNHFGPEGNYLGRYAPSFFSDAHTPWGSAIDYRVPEVRAFAIGSV 239 DEAHLRGLMV LDVVYNHFGPEGNYLG YAP FFSD HTPWG+AI+YRV EVRAFAI + Sbjct: 181 DEAHLRGLMVFLDVVYNHFGPEGNYLGGYAPDFFSDKHTPWGNAINYRVGEVRAFAIENA 240 Query: 240 VSWLRDYRFDGLRFDAVNTIVEAGEISVLHDFSRAAGKLATETGRDINLVLENGDNIASL 299 + WLRDYRFDGLR DAV+ I + GEI +LH+ SR AGKLA ETGR I+LVLEN DNIA++ Sbjct: 241 LHWLRDYRFDGLRLDAVHAIPDQGEIPMLHELSREAGKLAAETGRHIHLVLENDDNIAAV 300 Query: 300 LDATEDPPHGKFRGQWNDDYHHAWHVLLTGETQGYYGDYR-APKQDLARALSSGYIYQGE 358 LD DP G++R QWNDDYHHAWHV LTGE QGYY DY AP + LARAL SG+ YQGE Sbjct: 301 LDPVTDPSRGQYRAQWNDDYHHAWHVALTGERQGYYSDYADAPLRHLARALGSGFAYQGE 360 Query: 359 ISDFWGGKARGEPSGALSPTCFINFLQNHDQIGNRPLGDRLLSIARPEAV 408 S GG RGEPSG L P F+NFLQNHDQIGNR LGDRL S+A+P A+ Sbjct: 361 PSAHRGGTPRGEPSGELPPLGFVNFLQNHDQIGNRALGDRLESLAKPAAI 410