BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0012.p (336 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08629085.1| enoyl-(acyl-carrier-protein) reductase [Brady... 602 e-170 ref|NP_769246.1| 2-nitropropane dioxygenase [Bradyrhizobium japo... 592 e-167 ref|YP_001241048.1| putative 2-nitropropane dioxygenase [Bradyrh... 573 e-161 ref|YP_576383.1| 2-nitropropane dioxygenase, NPD [Nitrobacter ha... 571 e-161 ref|YP_002947223.1| 2-nitropropane dioxygenase NPD [Variovorax p... 562 e-158 >ref|ZP_08629085.1| enoyl-(acyl-carrier-protein) reductase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08444.1| enoyl-(acyl-carrier-protein) reductase [Bradyrhizobiaceae bacterium SG-6C] Length = 337 Score = 602 bits (1552), Expect = e-170, Method: Compositional matrix adjust. Identities = 291/337 (86%), Positives = 315/337 (93%), Gaps = 1/337 (0%) Query: 1 VKTAITELFGIQHPIIQGGMHYVGFAEMAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 +KTAITELFGIQHPIIQGGMHYVGFAE+A+AVSNAGGLGIITGLTQ+TPELLAKEIA+C+ Sbjct: 1 MKTAITELFGIQHPIIQGGMHYVGFAELASAVSNAGGLGIITGLTQKTPELLAKEIAKCK 60 Query: 61 DMTDKPIGVNLTFLPSFTAPPYPEYIAVIREGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 DMTDKP GVNLTFLP+F APPYPEYI VI+E G+KAVETAGRSPEQYMPALKAAGIKVIH Sbjct: 61 DMTDKPFGVNLTFLPTFAAPPYPEYIQVIKEAGIKAVETAGRSPEQYMPALKAAGIKVIH 120 Query: 121 KCTSVRHSLKAEKIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 KCTSVRHSLKAE IGCDAVSVDGFECGGHPGEDD+PNMILLPRA +EL IPFVASGGMAD Sbjct: 121 KCTSVRHSLKAEAIGCDAVSVDGFECGGHPGEDDMPNMILLPRAEEELTIPFVASGGMAD 180 Query: 181 ARSLVAALSMGAAGMNMGTRFIATKEAPVHPNVKQALLDADELDTRLVMRALRNTERVLK 240 ARSLVAAL+MGA GMNMGTRFIATKEAPVHPNVKQALL A ELDTRLVMRALRNTERVL Sbjct: 181 ARSLVAALAMGAQGMNMGTRFIATKEAPVHPNVKQALLKATELDTRLVMRALRNTERVLT 240 Query: 241 NKGVDELLEIEREKGARLKIEDIHEQVAGVYPKVMIDGEMDAGAWSCGMVVGLINDIPTV 300 NK V++L+EIE+EKG LKIEDI +QVAGVYPKVM++G+MDAGAWSCGM+VGLI+DIPTV Sbjct: 241 NKNVEKLIEIEKEKGKSLKIEDIMDQVAGVYPKVMLEGDMDAGAWSCGMMVGLIHDIPTV 300 Query: 301 KELIDRIMAEAEAIIRQRLTGFLDGKFE-TTPARAVA 336 KELIDRIMA+AE IIR+RLTGFLDG F TP R VA Sbjct: 301 KELIDRIMADAEFIIRKRLTGFLDGTFSAATPRRKVA 337 >ref|NP_769246.1| 2-nitropropane dioxygenase [Bradyrhizobium japonicum USDA 110] dbj|BAC47871.1| 2-nitropropane dioxygenase [Bradyrhizobium japonicum USDA 110] Length = 333 Score = 592 bits (1527), Expect = e-167, Method: Compositional matrix adjust. Identities = 284/329 (86%), Positives = 308/329 (93%) Query: 1 VKTAITELFGIQHPIIQGGMHYVGFAEMAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 +KTAITELFGI+HPIIQGGMH+VGFAE+AAAVSNAGGLGIITGLTQ+TPELLAKEIARCR Sbjct: 1 MKTAITELFGIEHPIIQGGMHFVGFAELAAAVSNAGGLGIITGLTQKTPELLAKEIARCR 60 Query: 61 DMTDKPIGVNLTFLPSFTAPPYPEYIAVIREGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 DMTDKP GVNLTFLP+F+APPYPEYIA I EGGVKAVETAGRSPEQYMPALKAAGIKVIH Sbjct: 61 DMTDKPFGVNLTFLPAFSAPPYPEYIAAIVEGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 Query: 121 KCTSVRHSLKAEKIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 KCTSVRHSLKAE+IGCDAVSVDGFECGGHPGEDDIPNMILLPRAA+ELKIPFVASGGMAD Sbjct: 121 KCTSVRHSLKAERIGCDAVSVDGFECGGHPGEDDIPNMILLPRAAEELKIPFVASGGMAD 180 Query: 181 ARSLVAALSMGAAGMNMGTRFIATKEAPVHPNVKQALLDADELDTRLVMRALRNTERVLK 240 RSLVAALS+GAAGMNMGTRFIATKEAPVH NVK AL+ A ELDTRL+MR+LRNTERVLK Sbjct: 181 GRSLVAALSLGAAGMNMGTRFIATKEAPVHQNVKNALVAATELDTRLIMRSLRNTERVLK 240 Query: 241 NKGVDELLEIEREKGARLKIEDIHEQVAGVYPKVMIDGEMDAGAWSCGMVVGLINDIPTV 300 N VD LLEIEREKG +L I+DIH+QVAGVYP++M+DG+MDAGAWSCGMV GLI+DIP+ Sbjct: 241 NANVDRLLEIEREKGDKLTIDDIHDQVAGVYPRIMLDGQMDAGAWSCGMVAGLIHDIPSC 300 Query: 301 KELIDRIMAEAEAIIRQRLTGFLDGKFET 329 KEL+DRIM+EAE IIR RL GFLDG T Sbjct: 301 KELVDRIMSEAETIIRGRLMGFLDGTGAT 329 >ref|YP_001241048.1| putative 2-nitropropane dioxygenase [Bradyrhizobium sp. BTAi1] gb|ABQ37142.1| 2-nitropropane dioxygenase precursor [Bradyrhizobium sp. BTAi1] Length = 334 Score = 573 bits (1477), Expect = e-161, Method: Compositional matrix adjust. Identities = 286/336 (85%), Positives = 314/336 (93%), Gaps = 2/336 (0%) Query: 1 VKTAITELFGIQHPIIQGGMHYVGFAEMAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 +KTAITE+ GI+HPI+QGGMHYVGFAE+AAAVSNAGGLGIITGLTQRTPELLAKEIARCR Sbjct: 1 MKTAITEMLGIEHPIVQGGMHYVGFAELAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 Query: 61 DMTDKPIGVNLTFLPSFTAPPYPEYIAVIREGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 D+TDKP GVNLTFLP+FTAPPYPEYIA I+EGGV+ VETAGRSPEQYMPALKAAGIKVIH Sbjct: 61 DVTDKPFGVNLTFLPTFTAPPYPEYIAAIKEGGVRVVETAGRSPEQYMPALKAAGIKVIH 120 Query: 121 KCTSVRHSLKAEKIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 KCTSVRHSLKA++IGCDAVSVDGFECGGHPGEDD+PNMILLPRAADEL IPF+ASGGMAD Sbjct: 121 KCTSVRHSLKAQQIGCDAVSVDGFECGGHPGEDDMPNMILLPRAADELTIPFIASGGMAD 180 Query: 181 ARSLVAALSMGAAGMNMGTRFIATKEAPVHPNVKQALLDADELDTRLVMRALRNTERVLK 240 RSLVAAL++GAAG+NMGTRFIATKEAPVH NVK ALL A ELDTRLVMRALRNTERVL Sbjct: 181 GRSLVAALALGAAGVNMGTRFIATKEAPVHQNVKDALLKATELDTRLVMRALRNTERVLT 240 Query: 241 NKGVDELLEIEREKGARLKIEDIHEQVAGVYPKVMIDGEMDAGAWSCGMVVGLINDIPTV 300 NKGV+ L+EIEREKG L I+DIH++VAGVYPKVM+DG+MDAGAWSCG+VVGLI+DIPTV Sbjct: 241 NKGVERLIEIEREKGKNLTIQDIHDEVAGVYPKVMVDGDMDAGAWSCGLVVGLIHDIPTV 300 Query: 301 KELIDRIMAEAEAIIRQRLTGFLDGKFETTPARAVA 336 KELIDRIMAEAE +IR RLTGFLDG + + AR VA Sbjct: 301 KELIDRIMAEAERLIRSRLTGFLDG--DISGARKVA 334 >ref|YP_576383.1| 2-nitropropane dioxygenase, NPD [Nitrobacter hamburgensis X14] gb|ABE61923.1| 2-nitropropane dioxygenase precursor [Nitrobacter hamburgensis X14] Length = 328 Score = 571 bits (1472), Expect = e-161, Method: Compositional matrix adjust. Identities = 274/326 (84%), Positives = 301/326 (92%) Query: 1 VKTAITELFGIQHPIIQGGMHYVGFAEMAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 +KTAITELFGIQHPI+QGGMHYVGFAE+A+AVSNAGGLGIITGLTQ+TP+LLAKEIARCR Sbjct: 1 MKTAITELFGIQHPIVQGGMHYVGFAELASAVSNAGGLGIITGLTQKTPDLLAKEIARCR 60 Query: 61 DMTDKPIGVNLTFLPSFTAPPYPEYIAVIREGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 MTDKP GVNLTFLP+FT PPYPEYI I EGG+ VETAGR+PEQYMPALKAAGI VIH Sbjct: 61 GMTDKPFGVNLTFLPAFTTPPYPEYIQAIVEGGIGIVETAGRNPEQYMPALKAAGITVIH 120 Query: 121 KCTSVRHSLKAEKIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 KCTSVRH+LKAE+IGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD Sbjct: 121 KCTSVRHALKAEQIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 Query: 181 ARSLVAALSMGAAGMNMGTRFIATKEAPVHPNVKQALLDADELDTRLVMRALRNTERVLK 240 ARSLVAAL++GA+GMNMGTRFIATKEAPVH NVK+AL+ A E DTRL+MRALRNTERVL Sbjct: 181 ARSLVAALALGASGMNMGTRFIATKEAPVHDNVKEALVGASERDTRLIMRALRNTERVLN 240 Query: 241 NKGVDELLEIEREKGARLKIEDIHEQVAGVYPKVMIDGEMDAGAWSCGMVVGLINDIPTV 300 N+ V+ L+EIEREKG LKI DI E+VAGVYPKVMIDG+MDAGAWSCGM VGLI+D+P+ Sbjct: 241 NRKVERLMEIEREKGESLKIGDIIEEVAGVYPKVMIDGDMDAGAWSCGMTVGLIHDVPSC 300 Query: 301 KELIDRIMAEAEAIIRQRLTGFLDGK 326 KELIDRIM +AE IIR+RLTG LDGK Sbjct: 301 KELIDRIMVDAERIIRRRLTGLLDGK 326 >ref|YP_002947223.1| 2-nitropropane dioxygenase NPD [Variovorax paradoxus S110] gb|ACS21957.1| 2-nitropropane dioxygenase NPD [Variovorax paradoxus S110] Length = 328 Score = 562 bits (1449), Expect = e-158, Method: Compositional matrix adjust. Identities = 268/324 (82%), Positives = 298/324 (91%) Query: 1 VKTAITELFGIQHPIIQGGMHYVGFAEMAAAVSNAGGLGIITGLTQRTPELLAKEIARCR 60 +KT ITELFGI+HPIIQGGMHYVGFAE+AAAVSNAGGLG+ITGLTQRTPELLAKEIARCR Sbjct: 1 MKTRITELFGIRHPIIQGGMHYVGFAELAAAVSNAGGLGLITGLTQRTPELLAKEIARCR 60 Query: 61 DMTDKPIGVNLTFLPSFTAPPYPEYIAVIREGGVKAVETAGRSPEQYMPALKAAGIKVIH 120 +MTD+P GVNLTFLP+ ++PPYPEYIA I EGGV+ VETAGRSPE YMPALK AGIKVIH Sbjct: 61 EMTDQPFGVNLTFLPTLSSPPYPEYIAAIAEGGVRIVETAGRSPEAYMPALKKAGIKVIH 120 Query: 121 KCTSVRHSLKAEKIGCDAVSVDGFECGGHPGEDDIPNMILLPRAADELKIPFVASGGMAD 180 KCTSVRH+LKAE IGCDAVSVDGFECGGHPGEDD+PNMILLPRAA+ELKIPFVASGGMAD Sbjct: 121 KCTSVRHALKAEAIGCDAVSVDGFECGGHPGEDDLPNMILLPRAAEELKIPFVASGGMAD 180 Query: 181 ARSLVAALSMGAAGMNMGTRFIATKEAPVHPNVKQALLDADELDTRLVMRALRNTERVLK 240 ARSLVAAL++GA GMNMGTRF+AT+EAP+H NVK A++ A ELDTRLVMR LRNTERVL Sbjct: 181 ARSLVAALALGAEGMNMGTRFVATQEAPIHQNVKDAIVAATELDTRLVMRGLRNTERVLT 240 Query: 241 NKGVDELLEIEREKGARLKIEDIHEQVAGVYPKVMIDGEMDAGAWSCGMVVGLINDIPTV 300 N V++LL IE+EKGA LK EDI +QVAGVYP+VM++GEMDAGAWSCGMVVGLINDIPTV Sbjct: 241 NASVEQLLRIEKEKGADLKFEDIIDQVAGVYPRVMLEGEMDAGAWSCGMVVGLINDIPTV 300 Query: 301 KELIDRIMAEAEAIIRQRLTGFLD 324 +ELIDRIM EAE +I QRL GFL+ Sbjct: 301 QELIDRIMEEAEQLIVQRLAGFLE 324