BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0007.p (514 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08630139.1| long-chain-fatty-acid--CoA ligase [Bradyrhizo... 701 0.0 ref|YP_003908041.1| AMP-dependent synthetase and ligase [Burkhol... 473 e-131 ref|ZP_02884670.1| AMP-dependent synthetase and ligase [Burkhold... 458 e-127 ref|ZP_03269745.1| AMP-dependent synthetase and ligase [Burkhold... 457 e-126 ref|YP_002912690.1| AMP-binding domain-containing protein [Burkh... 457 e-126 >ref|ZP_08630139.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07233.1| long-chain-fatty-acid--CoA ligase [Bradyrhizobiaceae bacterium SG-6C] Length = 524 Score = 701 bits (1809), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/501 (71%), Positives = 400/501 (79%) Query: 14 LPNRIHEVIDHHVGATPDHPALIDDKTRLTYRELDRTVGRVAEALRALGIRAGDRMMIVS 73 LP R+H+ + V ATPDHPALI+D +YR+L V + A +L ALGIR GDRMMIVS Sbjct: 23 LPERLHDTMARAVVATPDHPALIEDARTWSYRQLADNVEQTAASLTALGIRPGDRMMIVS 82 Query: 74 ENSIPLACLLLATSRLDVWSIVVNPRLSPRELDQIRDHSGARRVLLTAEVSQEAATHAAR 133 ENSI L LLLA S+++ W+IVVNPRLSPRELDQIRDHSG+RR+ TAEVS EAA HA R Sbjct: 83 ENSIALVALLLAASKINAWAIVVNPRLSPRELDQIRDHSGSRRMFFTAEVSAEAANHAQR 142 Query: 134 YEATVQDVGPLRGIAVTGLNLTTVAEPVEADGANQVAVLIYTSGTTGTPKGVMLTHRNLL 193 A VGPL GI V+ LN T EPVE+D A QVAVLIYTSGTTGTPKGVMLTH NLL Sbjct: 143 VGAQTLKVGPLEGIGVSALNEATTPEPVESDPAKQVAVLIYTSGTTGTPKGVMLTHENLL 202 Query: 194 FSARATASFRKMAADDVQYCVLPISHIVGISLLTMTLMIGATVRLVAKYDPAALVKAMTE 253 FSA+ TA RKM D Y VLPISHIVG+SL MTLM G TVRLV+KYDPAAL KA+ E Sbjct: 203 FSAKTTARLRKMDNRDKLYVVLPISHIVGVSLTLMTLMTGGTVRLVSKYDPAALAKAIAE 262 Query: 254 EGITILNGVPATYQRLLEYRRNAGLPKLDRGRLRVISVAGAPLDLDLKSRVEQELGLPLL 313 EGITILNGVPATYQRLLEY+ +G+ KL++G LR I VAGAPLD DLKSRVE ELGL LL Sbjct: 263 EGITILNGVPATYQRLLEYKTLSGVAKLNKGSLRYIGVAGAPLDPDLKSRVEAELGLSLL 322 Query: 314 NGYGITECSPGISGVRPDNPRADHAVGTVMPGLEAKLVGRDLKPVGAGEVGELHVRGPNV 373 NGYGITEC+PGISGVR + PR D AVG ++PG+EA+++ RD KP VGELHVRG NV Sbjct: 323 NGYGITECAPGISGVRIEAPRNDSAVGQIVPGVEARIIDRDGKPAANSAVGELHVRGRNV 382 Query: 374 MRGYYRAPDLTAKAIDPDGWFNTGDLARFENDALYIVGRTKEMIIRSGFNVYPAEIEAVL 433 MRGYYRAPDLT KAID DGWFNTGDLA + D ++IVGRTKEMIIRSGFNVYPAE+EAVL Sbjct: 383 MRGYYRAPDLTEKAIDRDGWFNTGDLAYVDGDCIFIVGRTKEMIIRSGFNVYPAEVEAVL 442 Query: 434 STHDAVVQCAVVGRPVEGNEEVVAFVQLIKGSTATVQDLMAHIAPQLTSYKRPSEIILMD 493 S H+ VVQ AVVGR +GNEEVVAFVQL+KGS T DLM QLTSYKRPS+II++D Sbjct: 443 SAHEDVVQSAVVGRAADGNEEVVAFVQLLKGSKVTPSDLMNFAGKQLTSYKRPSQIIVLD 502 Query: 494 ALPATSTGKLLKHKLAESLRG 514 ALPATSTGK+LKHKLAESLRG Sbjct: 503 ALPATSTGKILKHKLAESLRG 523 >ref|YP_003908041.1| AMP-dependent synthetase and ligase [Burkholderia sp. CCGE1003] gb|ADN58750.1| AMP-dependent synthetase and ligase [Burkholderia sp. CCGE1003] Length = 520 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 256/503 (50%), Positives = 330/503 (65%), Gaps = 4/503 (0%) Query: 13 GLPNRIHEVIDHHVGATPDHPALIDDKTRLTYRELDRTVGRVAEALRALGIRAGDRMMIV 72 LP+RI ++ P H ALI++ RLT EL V A LR G+R GDR+MIV Sbjct: 19 ALPDRISDLPTLAAARDPQHVALIEEARRLTNAELVNAVEAAAALLRQWGVRGGDRVMIV 78 Query: 73 SENSIPLACLLLATSRLDVWSIVVNPRLSPRELDQIRDHSGARRVLLTAEVSQEAATHAA 132 +ENSI LL AT+RLD W++V N RLS ELD IR H+ R E S++A HA Sbjct: 79 AENSIAQIVLLFATARLDAWALVSNARLSAAELDAIRAHAQPRVTAYAIESSRDAGQHAH 138 Query: 133 RY-EATVQDVGPLRGIAVTGLNLTTVAEPVEADGANQVAVLIYTSGTTGTPKGVMLTHRN 191 R+ A + P G ++ + AEPVEA Q A LIYT+GTTG PKGVML+HRN Sbjct: 139 RHGAAEAPHIAPDIGAWSYTVDASAQAEPVEAANHRQCAALIYTTGTTGAPKGVMLSHRN 198 Query: 192 LLFSARATASFRKMAADDVQYCVLPISHIVGISLLTM-TLMIGATVRLVAKYDPAALVKA 250 LLF A ++ R++ DDV Y VLPISH+ G + + + +L GAT+RL ++ P A+ +A Sbjct: 199 LLFIAAVSSRLRQVRPDDVVYAVLPISHVYGFASVCLGSLCAGATLRLAPRFAPEAVRRA 258 Query: 251 MTEEGITILNGVPATYQRLLEYRRNAGLPKLDRGRLRVISVAGAPLDLDLKSRVEQELGL 310 + +EG+TI GVPA + +LLE+ R G RLR + G+PLD LK++VE GL Sbjct: 259 LADEGVTIFQGVPAMHAKLLEHLRTQGH-AWHAPRLRFVYSGGSPLDAALKAQVEHVYGL 317 Query: 311 PLLNGYGITECSPGISGVRPDNPRADHAVGTVMPGLEAKLVGRDLKPVGAGEVGELHVRG 370 PL NGYG+TE SP +S D PR+D +VG V+PG+E + VG D GEVGEL VRG Sbjct: 318 PLHNGYGMTESSPTVSQTMLDAPRSDCSVGEVIPGVEVRFVGLDGVDAAPGEVGELWVRG 377 Query: 371 PNVMRGYYRAPDLTAKAIDPDGWFNTGDLARFEND-ALYIVGRTKEMIIRSGFNVYPAEI 429 PNVM GYYR+P+ T A+ +GW TGDLAR + D AL+IVGR+K++IIRSGFNVYPAE+ Sbjct: 378 PNVMLGYYRSPEQTRTAVTQEGWLKTGDLARQDPDGALHIVGRSKDLIIRSGFNVYPAEV 437 Query: 430 EAVLSTHDAVVQCAVVGRPVEGNEEVVAFVQLIKGSTATVQDLMAHIAPQLTSYKRPSEI 489 E VL+ H VVQ AV+GRPVEGNEEVVAFV+L +T T +L A A +L YKRP+E+ Sbjct: 438 EHVLNAHPQVVQSAVIGRPVEGNEEVVAFVELAAQATVTPAELAAWCAQRLAPYKRPAEV 497 Query: 490 ILMDALPATSTGKLLKHKLAESL 512 ++ ALPA STGK+LKH+L E L Sbjct: 498 KVLAALPAASTGKILKHRLREWL 520 >ref|ZP_02884670.1| AMP-dependent synthetase and ligase [Burkholderia graminis C4D1M] gb|EDT09835.1| AMP-dependent synthetase and ligase [Burkholderia graminis C4D1M] Length = 522 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 253/511 (49%), Positives = 329/511 (64%), Gaps = 10/511 (1%) Query: 9 GSVEGLPNRIHEVIDHHVGATPDHPALIDDKTRLTYRELDRTVGRVAEALRALGIRAGDR 68 G + LP RI ++ P H ALI+D RLT +L V V+ LR G+R GDR Sbjct: 15 GLLAALPARISDIPALAAARDPQHVALIEDARRLTNAQLMEAVDTVSALLREWGVRGGDR 74 Query: 69 MMIVSENSIPLACLLLATSRLDVWSIVVNPRLSPRELDQIRDHSGARRVLLTAEVSQEAA 128 +MIV+ENSI LL AT+RLD W++V N RLS ELD IR H+ R E S +A+ Sbjct: 75 VMIVAENSIAQIVLLFATARLDAWALVSNARLSAAELDAIRAHAKPRVTAYMVESSVDAS 134 Query: 129 THAARYEA-----TVQDVGPLRGIAVTGLNLTTVAEPVEADGANQVAVLIYTSGTTGTPK 183 HA R++A D+G +A + T AEPVEA Q A LIYT+GTTG PK Sbjct: 135 QHAERHQAREAPGMTPDIGAWSYVADD--SAQTQAEPVEAASERQCAALIYTTGTTGAPK 192 Query: 184 GVMLTHRNLLFSARATASFRKMAADDVQYCVLPISHIVGISLLTM-TLMIGATVRLVAKY 242 GVML+HRNLLF A ++ R++ DDV Y VLPISH+ G + + + +L GAT+RL ++ Sbjct: 193 GVMLSHRNLLFIATVSSRLRQVRPDDVVYAVLPISHVYGFASVCLGSLHAGATLRLAPRF 252 Query: 243 DPAALVKAMTEEGITILNGVPATYQRLLEYRRNAGLPKLDRGRLRVISVAGAPLDLDLKS 302 P A+ +A+T+E ++I GVPA + +LLE+ + G + LR + G+PLD LK+ Sbjct: 253 APEAVRRALTDERVSIFQGVPAMHAKLLEHLQTHG-HEWRAPHLRFVYSGGSPLDAALKA 311 Query: 303 RVEQELGLPLLNGYGITECSPGISGVRPDNPRADHAVGTVMPGLEAKLVGRDLKPVGAGE 362 VE GLPL NGYG+TE SP +S D PRAD +VG V+PG+E + V D G Sbjct: 312 HVESVYGLPLHNGYGMTESSPTVSQTMLDAPRADCSVGEVIPGVEVRFVELDGADAAPGA 371 Query: 363 VGELHVRGPNVMRGYYRAPDLTAKAIDPDGWFNTGDLARFEND-ALYIVGRTKEMIIRSG 421 +GEL VRGPNVM GYYR P+ T A+ DGW TGDLAR + D AL+IVGR+K++IIRSG Sbjct: 372 IGELWVRGPNVMLGYYRNPEQTRAAVTEDGWLKTGDLARQDADGALHIVGRSKDLIIRSG 431 Query: 422 FNVYPAEIEAVLSTHDAVVQCAVVGRPVEGNEEVVAFVQLIKGSTATVQDLMAHIAPQLT 481 FNVYPAE+E VL+ H VVQ AV+GR VEGNEEV+AFV+L +T T +L A A +L Sbjct: 432 FNVYPAEVEHVLNAHPQVVQSAVIGRAVEGNEEVIAFVELAAHATVTPVELAAWCAERLA 491 Query: 482 SYKRPSEIILMDALPATSTGKLLKHKLAESL 512 YKRP+E+ ++ ALPA STGK+LKH+L E L Sbjct: 492 PYKRPAEVKVLAALPAASTGKILKHRLRELL 522 >ref|ZP_03269745.1| AMP-dependent synthetase and ligase [Burkholderia sp. H160] gb|EDZ98666.1| AMP-dependent synthetase and ligase [Burkholderia sp. H160] Length = 520 Score = 457 bits (1176), Expect = e-126, Method: Compositional matrix adjust. Identities = 251/501 (50%), Positives = 328/501 (65%), Gaps = 4/501 (0%) Query: 13 GLPNRIHEVIDHHVGATPDHPALIDDKTRLTYRELDRTVGRVAEALRALGIRAGDRMMIV 72 LP RI ++ P H ALI+D RL+ +L V A LR G+R GDR+MIV Sbjct: 19 ALPARISDIPALAASRDPQHVALIEDARRLSNAQLLLAVDAAAALLRDWGVRGGDRVMIV 78 Query: 73 SENSIPLACLLLATSRLDVWSIVVNPRLSPRELDQIRDHSGARRVLLTAEVSQEAATHAA 132 +ENSI LL AT++LD W++V N RLS ELD IR H+ R V E S +A HA Sbjct: 79 AENSIAQIALLFATAKLDAWALVSNARLSAAELDSIRAHAQPRVVAYAVESSPDARQHAE 138 Query: 133 RYEATVQ-DVGPLRGIAVTGLNLTTVAEPVEADGANQVAVLIYTSGTTGTPKGVMLTHRN 191 R++AT + + P G ++ + AEPVEA Q A LIYT+GTTG PKGVML+HRN Sbjct: 139 RHQATHEPTLSPDIGAWSYAVDSSAQAEPVEAANDRQCAALIYTTGTTGAPKGVMLSHRN 198 Query: 192 LLFSARATASFRKMAADDVQYCVLPISHIVGISLLTM-TLMIGATVRLVAKYDPAALVKA 250 LL+ A ++ R++ DDV Y VLPISH+ G + + + +L GAT+RL ++ P A+ +A Sbjct: 199 LLYIAAVSSRLRQVGPDDVVYAVLPISHVYGFASVCLGSLHAGATLRLAPRFVPEAVRRA 258 Query: 251 MTEEGITILNGVPATYQRLLEYRRNAGLPKLDRGRLRVISVAGAPLDLDLKSRVEQELGL 310 + +E ++I GVPA + +LLE+ G RLR G+PLD LK++ E GL Sbjct: 259 LADERVSIFQGVPAMHAKLLEHLHTHGH-AWSAPRLRFAYSGGSPLDAALKAQAEAVYGL 317 Query: 311 PLLNGYGITECSPGISGVRPDNPRADHAVGTVMPGLEAKLVGRDLKPVGAGEVGELHVRG 370 PL NGYG+TE SP +S D PR+D +VG V+PG+E K VG D GE+GEL VRG Sbjct: 318 PLHNGYGMTESSPTVSHTMLDAPRSDCSVGEVIPGVEVKFVGLDGVEAAPGEIGELWVRG 377 Query: 371 PNVMRGYYRAPDLTAKAIDPDGWFNTGDLARFEND-ALYIVGRTKEMIIRSGFNVYPAEI 429 PNVM GYYR+P+ T A+ DGW TGDLAR + D AL+IVGR+KE+IIRSGFNVYPAE+ Sbjct: 378 PNVMLGYYRSPEQTRAALTEDGWLKTGDLARQDEDGALHIVGRSKELIIRSGFNVYPAEV 437 Query: 430 EAVLSTHDAVVQCAVVGRPVEGNEEVVAFVQLIKGSTATVQDLMAHIAPQLTSYKRPSEI 489 E VL+ H VVQ AV+GR VEGNEEV+AFV+LI G+T T +L+A +L YKRP+E+ Sbjct: 438 EHVLNAHPQVVQSAVIGRAVEGNEEVIAFVELISGATVTPAELIAWCGERLAPYKRPAEL 497 Query: 490 ILMDALPATSTGKLLKHKLAE 510 ++ ALPA STGK+LKH+L E Sbjct: 498 KVLAALPAASTGKILKHRLRE 518 >ref|YP_002912690.1| AMP-binding domain-containing protein [Burkholderia glumae BGR1] gb|ACR29986.1| AMP-binding domain-containing protein [Burkholderia glumae BGR1] Length = 523 Score = 457 bits (1176), Expect = e-126, Method: Compositional matrix adjust. Identities = 247/499 (49%), Positives = 324/499 (64%), Gaps = 4/499 (0%) Query: 15 PNRIHEVIDHHVGATPDHPALIDDKTRLTYRELDRTVGRVAEALRALGIRAGDRMMIVSE 74 P RI + PDHPAL++D RL YR+L + A LRALG+R GDR+MIV+E Sbjct: 20 PERITAIPARIAARQPDHPALVEDARRLNYRQLAAAIEATAGQLRALGVRGGDRVMIVAE 79 Query: 75 NSIPLACLLLATSRLDVWSIVVNPRLSPRELDQIRDHSGARRV-LLTAEVSQEAATHAAR 133 NSI LL AT+ LD W+ + N RLS ELD I H+ R + +TA S AA A Sbjct: 80 NSIAQIVLLFATASLDAWACLANARLSAAELDAIAAHARPRAIAFVTATSSDAAAHAARH 139 Query: 134 YEATVQDVGPLRGIAVTGLNLTTVAEPVEADGANQVAVLIYTSGTTGTPKGVMLTHRNLL 193 + P G L+ AEPVE+ A Q A LIYT+GTTG PKGVML+HRNLL Sbjct: 140 RALHAAEGEPGIGTWAVALDPDAGAEPVESGAARQCAALIYTTGTTGAPKGVMLSHRNLL 199 Query: 194 FSARATASFRKMAADDVQYCVLPISHIVGISLLTM-TLMIGATVRLVAKYDPAALVKAMT 252 F A +++ R+++ DDV Y LP+SH+ G++ + + +L GAT+RLV ++ P AL +A+ Sbjct: 200 FVAAMSSTLRRVSPDDVVYAALPVSHVYGLTSVCLGSLYAGATLRLVQRFTPEALRRALA 259 Query: 253 EEGITILNGVPATYQRLLEYRRNAGLPKLDRGRLRVISVAGAPLDLDLKSRVEQELGLPL 312 +E +TI GVPA + +LL++ + G LR + G+PLD DLK+RVE+ GLP+ Sbjct: 260 DERVTIFQGVPAMHAKLLDHLQAHGH-AWHAPHLRFVYSGGSPLDADLKARVERIYGLPI 318 Query: 313 LNGYGITECSPGISGVRPDNPRADHAVGTVMPGLEAKLVGRDLKPVGAGEVGELHVRGPN 372 NGYG+TE SP ++ D PRAD +VGT +PG+E +L G D GEVGE+ VRGPN Sbjct: 319 HNGYGMTESSPTVAQTSLDAPRADCSVGTPIPGIEVRLAGVDGCDAAPGEVGEILVRGPN 378 Query: 373 VMRGYYRAPDLTAKAIDPDGWFNTGDLARFEND-ALYIVGRTKEMIIRSGFNVYPAEIEA 431 VM GYYR P+ T A+ DGW TGDLA +D +L +VGR+KE+IIRSGFNVYPAE+E Sbjct: 379 VMLGYYRDPEATRAAVTADGWLKTGDLASAADDGSLTLVGRSKELIIRSGFNVYPAEVEH 438 Query: 432 VLSTHDAVVQCAVVGRPVEGNEEVVAFVQLIKGSTATVQDLMAHIAPQLTSYKRPSEIIL 491 L+ H AVVQ AV+GRPV GNEEV+AFV+L G+ +L A A +L YKRP+ I+ Sbjct: 439 ALNAHPAVVQSAVIGRPVAGNEEVMAFVELTPGAALASDELAAWCAARLAPYKRPARIVA 498 Query: 492 MDALPATSTGKLLKHKLAE 510 +DALPA STGK+LKH+L E Sbjct: 499 VDALPAASTGKVLKHRLRE 517