BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0134.0002.p (423 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772552.1| serine hydroxymethyltransferase [Bradyrhizobium... 725 0.0 ref|YP_002952869.1| serine hydroxymethyltransferase [Desulfovibr... 613 e-173 ref|YP_002990906.1| glycine hydroxymethyltransferase [Desulfovib... 597 e-168 ref|YP_001411888.1| glycine hydroxymethyltransferase [Parvibacul... 590 e-166 ref|YP_003368649.1| glycine hydroxymethyltransferase [Pirellula ... 441 e-121 >ref|NP_772552.1| serine hydroxymethyltransferase [Bradyrhizobium japonicum USDA 110] dbj|BAC51177.1| serine hydroxymethyltransferase [Bradyrhizobium japonicum USDA 110] Length = 460 Score = 725 bits (1872), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/419 (84%), Positives = 385/419 (91%) Query: 1 MPSFRNELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYP 60 MPSFRNEL +ND E+A AL GEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYP Sbjct: 32 MPSFRNELSINDQEIAAALVGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYP 91 Query: 61 GRRYYGGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMD 120 GRRYYGGQ++TD+IE LAR+RAC+LFRAEHANVQPLSGSPMNQAVYLGLL+PGDTILAMD Sbjct: 92 GRRYYGGQQYTDEIERLARERACSLFRAEHANVQPLSGSPMNQAVYLGLLEPGDTILAMD 151 Query: 121 LSHGGHLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYP 180 LSHGGHLTHGAPVSHMGRLFNF RYKT PSNGGAIDFD LRA+AR RPK+VLCGYSSYP Sbjct: 152 LSHGGHLTHGAPVSHMGRLFNFIRYKTAPSNGGAIDFDELRAIAREARPKMVLCGYSSYP 211 Query: 181 RDLDYASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGG 240 RDLDYA+FKSIADEVGALTMADVSHYGGL+A N +RNPLDAGFDVMTTTSHKTLRGPRGG Sbjct: 212 RDLDYAAFKSIADEVGALTMADVSHYGGLVAANVMRNPLDAGFDVMTTTSHKTLRGPRGG 271 Query: 241 IVLCRREFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAE 300 I+LCR+E AG+IDASVFPGLQGGPHMNVVAG AVTLKKA+TSDFQ YA V+RNAKVLA Sbjct: 272 IILCRKENAGRIDASVFPGLQGGPHMNVVAGIAVTLKKAATSDFQVYARQVLRNAKVLAG 331 Query: 301 ALMERGMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKP 360 ALMERGM L+T GTDNHMMVVDT ASVGLDG AAE++LDA+ IT NKQ+IPDDPRPPL+P Sbjct: 332 ALMERGMKLVTDGTDNHMMVVDTAASVGLDGRAAEDVLDAIAITTNKQVIPDDPRPPLRP 391 Query: 361 SGIRLGTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPVPGI 419 SGIRLGTPAATTRGMGEPEMR I E IA +L++ G+ VV RLRS VEMCRAFPVPG+ Sbjct: 392 SGIRLGTPAATTRGMGEPEMRRIGEFIAAALQANGNDVVVARLRSECVEMCRAFPVPGV 450 >ref|YP_002952869.1| serine hydroxymethyltransferase [Desulfovibrio magneticus RS-1] dbj|BAH74983.1| serine hydroxymethyltransferase [Desulfovibrio magneticus RS-1] Length = 419 Score = 613 bits (1581), Expect = e-173, Method: Compositional matrix adjust. Identities = 298/419 (71%), Positives = 338/419 (80%), Gaps = 1/419 (0%) Query: 1 MPSFRNELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYP 60 M +F + L D EV L GEE RQ+ G+ELIPSENYTYPEVL LGSVFTNKYSEGYP Sbjct: 1 MRAFYDLLSATDPEVFATLAGEENRQRLGIELIPSENYTYPEVLAALGSVFTNKYSEGYP 60 Query: 61 GRRYYGGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMD 120 GRRYYGGQEFTDQIENLARQRACA+F EHANVQPLSGSPMNQAVYLGLL+PGDTILAMD Sbjct: 61 GRRYYGGQEFTDQIENLARQRACAVFGCEHANVQPLSGSPMNQAVYLGLLEPGDTILAMD 120 Query: 121 LSHGGHLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYP 180 LSHGGHLTHGAPVSHMGRLFNF RYKT P +G AIDFD +RA+AR +P+LVLCGY+SYP Sbjct: 121 LSHGGHLTHGAPVSHMGRLFNFVRYKTNPGDG-AIDFDVVRALAREHKPRLVLCGYTSYP 179 Query: 181 RDLDYASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGG 240 RDLDYA+FK+IADEVGA TM D SHY GL+AG +RNP DAGFDV+TTTSHK+LRGPRGG Sbjct: 180 RDLDYAAFKAIADEVGAYTMCDASHYAGLVAGGVMRNPFDAGFDVVTTTSHKSLRGPRGG 239 Query: 241 IVLCRREFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAE 300 ++LCR+ ID SVFPGLQGGPHMNV+AG A+TL KA +F+ YA V+ NA+ L Sbjct: 240 MILCRKALGPAIDKSVFPGLQGGPHMNVIAGIAITLGKALAPEFKVYAAQVLANARRLGA 299 Query: 301 ALMERGMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKP 360 L RG++LITGGTDNHM+V +T AS GLDG AE +LD G+T NKQIIPDDP PPL+P Sbjct: 300 ELAGRGVSLITGGTDNHMLVANTQASFGLDGRTAEELLDEAGLTTNKQIIPDDPNPPLRP 359 Query: 361 SGIRLGTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPVPGI 419 SGIRLGTPAATTRGMGE EM +A IA L S DAA +R+ ++C FPVPG+ Sbjct: 360 SGIRLGTPAATTRGMGEAEMVKLAGWIAEVLASPTDAAKRAAVRAEVAQLCGRFPVPGL 418 >ref|YP_002990906.1| glycine hydroxymethyltransferase [Desulfovibrio salexigens DSM 2638] gb|ACS79367.1| Glycine hydroxymethyltransferase [Desulfovibrio salexigens DSM 2638] Length = 418 Score = 597 bits (1538), Expect = e-168, Method: Compositional matrix adjust. Identities = 284/419 (67%), Positives = 334/419 (79%), Gaps = 1/419 (0%) Query: 1 MPSFRNELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYP 60 M +RN L ND ++ AL GEE RQ+ G+ELIPSENYTYPEVL LGSVFTNKYSEGYP Sbjct: 1 MQQYRNLLQANDPDIFNALSGEESRQRAGIELIPSENYTYPEVLCTLGSVFTNKYSEGYP 60 Query: 61 GRRYYGGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMD 120 GRRYYGGQEFTD IE++AR+RA +FR EHANVQPLSGSPMNQAVYLGLL+PGDTILAMD Sbjct: 61 GRRYYGGQEFTDTIEDIARERAKQVFRCEHANVQPLSGSPMNQAVYLGLLEPGDTILAMD 120 Query: 121 LSHGGHLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYP 180 LSHGGHLTHGAPVS MG+LFNF RYKT P +G +IDFD LR A +PK++LCGY+SYP Sbjct: 121 LSHGGHLTHGAPVSFMGKLFNFIRYKTDPVDG-SIDFDELRKTALEHKPKMILCGYTSYP 179 Query: 181 RDLDYASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGG 240 RDLDYA+FK IADEVGA+TM D SHYGGLIA + +RNP D GFDV+T+TSHK+LRGPRGG Sbjct: 180 RDLDYAAFKKIADEVGAITMTDASHYGGLIAADVIRNPFDFGFDVVTSTSHKSLRGPRGG 239 Query: 241 IVLCRREFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAE 300 ++LC++EFA +ID +VFPGLQGGPHMN +AG AVTLKKA +F+ Y V+ NAK LA+ Sbjct: 240 MILCKKEFAPRIDKAVFPGLQGGPHMNSIAGIAVTLKKALEPEFKEYGKQVLVNAKTLAD 299 Query: 301 ALMERGMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKP 360 L++ G +L+TGGTDNHMMV+DT S G++G AE +LD V IT NKQIIPDDP PPLKP Sbjct: 300 ELLKSGASLVTGGTDNHMMVLDTEKSYGINGKVAEELLDEVAITTNKQIIPDDPNPPLKP 359 Query: 361 SGIRLGTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPVPGI 419 SGIR+GTPAAT+RGM E +M +A I L++ D + RS C FPVPGI Sbjct: 360 SGIRIGTPAATSRGMKEADMVKLAGWITTILQNPEDKNLAVTTRSEIESFCSRFPVPGI 418 >ref|YP_001411888.1| glycine hydroxymethyltransferase [Parvibaculum lavamentivorans DS-1] gb|ABS62231.1| Glycine hydroxymethyltransferase [Parvibaculum lavamentivorans DS-1] Length = 420 Score = 590 bits (1522), Expect = e-166, Method: Compositional matrix adjust. Identities = 299/415 (72%), Positives = 346/415 (83%), Gaps = 1/415 (0%) Query: 5 RNELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRY 64 R L +D+E+ LAL+GEE RQ+ G+ELIPSENY +PEVL LLGS FTNKYSEGYPGRRY Sbjct: 7 RARLAQSDNEIFLALKGEEARQRAGLELIPSENYAFPEVLTLLGSAFTNKYSEGYPGRRY 66 Query: 65 YGGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHG 124 YGGQ++TD+IE LAR RA ALFRAEHANVQPLSGSPMNQAVYLGLL+PGDTILAMDLSHG Sbjct: 67 YGGQDYTDRIETLARDRAKALFRAEHANVQPLSGSPMNQAVYLGLLEPGDTILAMDLSHG 126 Query: 125 GHLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLD 184 GHLTHGAPVSHMGRLFNF RYKT P+NG AIDFD LRA+AR +P+LV+CGYSSYPRD D Sbjct: 127 GHLTHGAPVSHMGRLFNFVRYKTDPTNG-AIDFDHLRAVARETKPRLVVCGYSSYPRDYD 185 Query: 185 YASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLC 244 YA FK +ADEVGALTMADVSH GGLIA N +RNPLDAGFDVMTTT+HK+LRGPRGG++LC Sbjct: 186 YADFKRVADEVGALTMADVSHIGGLIAANVMRNPLDAGFDVMTTTTHKSLRGPRGGLILC 245 Query: 245 RREFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALME 304 + EFA KIDASVFPGLQGGPHMN VA AVT + A+ F+ YA V+ NAK LA ALME Sbjct: 246 KAEFARKIDASVFPGLQGGPHMNQVAAAAVTFRLAAMPAFRDYAEQVLANAKALAAALME 305 Query: 305 RGMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIR 364 R + L+TGGTDNH++V+DTVAS G+DG A+ LD VG+T NKQ+IPDDP PP++PSG+R Sbjct: 306 RQVKLVTGGTDNHLLVIDTVASFGIDGAVAQEALDRVGLTTNKQVIPDDPNPPMRPSGLR 365 Query: 365 LGTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPVPGI 419 LGTPAAT RGMG EM IAE+IA +L +KGDAA L + + ++ FPV G+ Sbjct: 366 LGTPAATARGMGHKEMAEIAEIIAAALNAKGDAATETALAARTHKLTSRFPVDGL 420 >ref|YP_003368649.1| glycine hydroxymethyltransferase [Pirellula staleyi DSM 6068] gb|ADB14789.1| Glycine hydroxymethyltransferase [Pirellula staleyi DSM 6068] Length = 419 Score = 441 bits (1134), Expect = e-121, Method: Compositional matrix adjust. Identities = 218/412 (52%), Positives = 287/412 (69%), Gaps = 3/412 (0%) Query: 6 NELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRYY 65 N L D +V A+ E+ RQQDG+E+I SENYT V++ +GSV TNKY+EGYPGRRYY Sbjct: 3 NVLSQQDPQVWAAIAAEQERQQDGLEMIASENYTSVAVMQAVGSVLTNKYAEGYPGRRYY 62 Query: 66 GGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHGG 125 GG E D IENLAR RA LF AEHANVQP SGS NQAVYL L+ PGDT+L +DL+HGG Sbjct: 63 GGCEHVDVIENLARDRAKQLFGAEHANVQPHSGSQANQAVYLSLINPGDTVLGLDLAHGG 122 Query: 126 HLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLDY 185 HLTHG ++ G+L+NF Y + S+ +DFD + +AR +PKL++ G S+YPR++ + Sbjct: 123 HLTHGMKLNLSGKLYNFHSYGVRQSD-HRLDFDQVARLAREHKPKLIVAGASAYPREIPH 181 Query: 186 ASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLCR 245 F IA EVGA D++HY GL+A NP+ D +TTT+HKTLRGPR G+VLC+ Sbjct: 182 GKFAEIAREVGAKLFVDMAHYAGLVAAGLHDNPVPVA-DFVTTTTHKTLRGPRAGLVLCK 240 Query: 246 REFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALMER 305 E+A +ID +VFPG+QGGP M+V+AG AV +A DF+AY ++ NAK LAE LM Sbjct: 241 AEYAKEIDKNVFPGMQGGPLMHVIAGKAVCFGEALQPDFKAYGQAILDNAKTLAETLMAG 300 Query: 306 GMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIRL 365 G++L++GGT+NH+M+VD V ++G+ G A +L GIT N +IP D R P+ PSG+R+ Sbjct: 301 GLSLVSGGTENHLMLVD-VTTLGIGGKLATEVLGHCGITVNMNMIPFDTRKPMDPSGVRI 359 Query: 366 GTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPVP 417 GTPA TTRGMG EM+ I I SL++ GDA+V +R+R +C FPVP Sbjct: 360 GTPALTTRGMGTDEMKTIGGWILESLKNCGDASVHQRIRGEVASLCSQFPVP 411