BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0034.p (264 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001021848.1| 3-hydroxybutyryl-CoA dehydratase [Methylibiu... 166 3e-39 ref|ZP_01625335.1| probable enoyl-CoA hydratase/isomerase [marin... 153 3e-35 ref|ZP_02887224.1| Enoyl-CoA hydratase/isomerase [Burkholderia g... 151 8e-35 ref|ZP_04320977.1| Enoyl-CoA hydratase/isomerase [Bacillus cereu... 151 9e-35 ref|ZP_03233903.1| enoyl-CoA hydratase/isomerase family protein ... 149 4e-34 >ref|YP_001021848.1| 3-hydroxybutyryl-CoA dehydratase [Methylibium petroleiphilum PM1] gb|ABM95613.1| 3-hydroxybutyryl-CoA dehydratase [Methylibium petroleiphilum PM1] Length = 269 Score = 166 bits (420), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 106/254 (41%), Positives = 138/254 (54%), Gaps = 5/254 (1%) Query: 1 MPLVSIQDRDAVRVITYANVPFGTMTAAGAAEMFDAVAAARMAPSVRVIVITGGLPGIFI 60 M L + D V Y N P A GAAE+ V + R P+V+ +V++GG+ G FI Sbjct: 1 MSLWTCTLDDGVVTAIYRNPPMNYFCAEGAAELGALVESWR-DPAVKAVVLSGGMAGRFI 59 Query: 61 RHYDVAELSEAADAIERGASRAPSSPGPRPPGFHALTDAIAAVDKPVIAAINGLCMGGGF 120 HY V EL A+ +R A R + G+HAL + + KPVI A+NG MGGGF Sbjct: 60 THYSVEELLSYAE--DRAAMRQAGTA--LNDGYHALLLGLRDLPKPVIVAMNGDAMGGGF 115 Query: 121 ELSLACDLRIAGSSVTAIGLPETRIGIFPGGGGTQRLPRIVGEARALEMILRGLTVTGQR 180 EL LACDLRIA GLPE ++GI PGG GTQRL R++G RA+E ILRG V Sbjct: 116 ELCLACDLRIAQRGDFRYGLPEVKLGILPGGSGTQRLSRLIGAGRAIEFILRGRIVEPAV 175 Query: 181 AHEIGIVHEVVADPLARSLEIGAELSGRSAEGLAFAKRLTRSALDRPLAEGLADERKSFS 240 A E+G+VHE+ D L R+ +I E+ +A AKR D LA GL E + F Sbjct: 176 ALEMGLVHELADDALRRAQQIAHEMVLTPPFAMAMAKRAVYEGSDTSLAAGLKLEGERFL 235 Query: 241 TVMRTASAREGLRA 254 M + + +RA Sbjct: 236 ETMLSDGSVSAMRA 249 >ref|ZP_01625335.1| probable enoyl-CoA hydratase/isomerase [marine gamma proteobacterium HTCC2080] gb|EAW41988.1| probable enoyl-CoA hydratase/isomerase [marine gamma proteobacterium HTCC2080] Length = 275 Score = 153 bits (386), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 99/258 (38%), Positives = 134/258 (51%), Gaps = 9/258 (3%) Query: 14 VITYANVPFGTMTAAGAAEMFDAVAAARMAPSVRVIVITGGLPGIFIRHYDVAELSEAAD 73 T +N P T+TA G +E+ + SVRV+V TG G+FI HY+V ELS++A Sbjct: 16 TCTLSNPPTQTLTAGGVSEIHQVLDDIESDRSVRVLVFTGAGDGVFIAHYEVGELSDSAQ 75 Query: 74 AIERGASRAPSS--PGPRPPGFHALTDAIAAVDKPVIAAINGLCMGGGFELSLACDLRIA 131 SR ++ P H L + + IAA+NG GGGFEL LACD R+ Sbjct: 76 RNIETDSRTITTGESEPELSEMHQLCLRLEQISAITIAAMNGTATGGGFELCLACDFRLL 135 Query: 132 GSSVTAIGLPETRIGIFPGGGGTQRLPRIVGEARALEMILRGLTVTGQRAHEIGIVHEV- 190 +GLPET IGI PG GGTQR R++G ARAL++IL +T +A E+G+VH Sbjct: 136 ADGRYRVGLPETSIGIIPGAGGTQRYARLLGTARALDLILHAKLLTPAQALEMGLVHRTY 195 Query: 191 -VADPLARSLEIGAELSGRSAEGLAFAKRLTRSALDRPLAEGLADERKSFSTVMRTASAR 249 V L E +++GRS LA AK+ + PL E L E++ F MR+ A Sbjct: 196 PVDCFLDEVEEFSVDIAGRSPLALAAAKQAIQQGARLPLDEALLLEQRHFDRTMRSKDAA 255 Query: 250 EGLRA-----GRPPAEIQ 262 +RA + P E Q Sbjct: 256 GAMRAYLNGSTKTPYEWQ 273 >ref|ZP_02887224.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] gb|EDT07183.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] Length = 266 Score = 151 bits (382), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 102/253 (40%), Positives = 130/253 (51%), Gaps = 1/253 (0%) Query: 1 MPLVSIQDRDAVRVITYANVPFGTMTAAGAAEMFDAVAAARMAPSVRVIVITGGLPGIFI 60 M V + D+ I+ +N P G MT E+ A AA PSVR I+ TG G+FI Sbjct: 1 MSEVRTRIYDSAAAISVSNPPHGYMTHELVGELLRAFRAAERDPSVRTIIFTGANAGVFI 60 Query: 61 RHYDVAELSEAADAIERGASRAPSSPGPRPPGFHALTDAIAAVDKPVIAAINGLCMGGGF 120 +HYD+AEL+ + + R P L I KPVIAAING MG G Sbjct: 61 QHYDLAELATLSRKLNARGDRYPEHAHTPEREMDVLFRRIEDSAKPVIAAINGNAMGFGC 120 Query: 121 ELSLACDLRIAGSSVTAIGLPETRIGIFPGGGGTQRLPRIVGEARALEMILRGLTVTGQR 180 EL+LACD RIA S +G PE +G+ PG GGTQRL R+VG A ALE+++ G ++ Sbjct: 121 ELALACDFRIAESGDHMLGQPEILVGLVPGAGGTQRLARVVGTACALELVVFGKRLSPAE 180 Query: 181 AHEIGIVHEVV-ADPLARSLEIGAELSGRSAEGLAFAKRLTRSALDRPLAEGLADERKSF 239 A G+VH V L +LE + L SA +A AKRL A L EGL ER F Sbjct: 181 ALAKGLVHAVSEGSALELALEWASVLGRLSATAVAHAKRLVHEAGVTQLHEGLQKERALF 240 Query: 240 STVMRTASAREGL 252 + + A E L Sbjct: 241 VDTLASPEANECL 253 >ref|ZP_04320977.1| Enoyl-CoA hydratase/isomerase [Bacillus cereus ATCC 10876] gb|EEK47315.1| Enoyl-CoA hydratase/isomerase [Bacillus cereus ATCC 10876] Length = 263 Score = 151 bits (382), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 87/254 (34%), Positives = 137/254 (53%), Gaps = 10/254 (3%) Query: 3 LVSIQDRDAVRVITYANVPFGTMTAAGAAEMFDAVAAARMAPSVRVIVITGGLPGIFIRH 62 +V+ + ++ V+T N P T++ ++ D + + + V++ITG F+ Sbjct: 6 VVTCNKKGSIAVVTIQNPPVNTLSLEVVQQLVDVLEEIEIDNDISVVIITGSGEKAFVAG 65 Query: 63 YDVAELSEAADAIERGASRAPSSPGPRPPGFHALTDAIAAVDKPVIAAINGLCMGGGFEL 122 ++ E D + +G A + H + + KP IAA+NGL +GGG EL Sbjct: 66 GNIKEFP---DWMGKGEKYAEM----KSLELHQPLKQLENLSKPTIAAVNGLALGGGCEL 118 Query: 123 SLACDLRIAGSSVTAIGLPETRIGIFPGGGGTQRLPRIVGEARALEMILRGLTVTGQRAH 182 +LACDLRI + IGLPE +G+FPG GGTQRLPR++GE +A EM+ G ++T + A Sbjct: 119 ALACDLRIIEEQ-SLIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKSITAKEAK 177 Query: 183 EIGIVHEVV--ADPLARSLEIGAELSGRSAEGLAFAKRLTRSALDRPLAEGLADERKSFS 240 EIG+V+ + + L ++ EI ++S S LA+ K R PL EGL E + F Sbjct: 178 EIGLVNYITPRGEALNKAKEIARDISKFSLPALAYMKLAIREGAAVPLQEGLEIEARYFG 237 Query: 241 TVMRTASAREGLRA 254 V +T +EG++A Sbjct: 238 KVFQTEDVKEGVKA 251 >ref|ZP_03233903.1| enoyl-CoA hydratase/isomerase family protein [Bacillus cereus AH1134] gb|EDZ49065.1| enoyl-CoA hydratase/isomerase family protein [Bacillus cereus AH1134] Length = 263 Score = 149 bits (376), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 135/254 (53%), Gaps = 10/254 (3%) Query: 3 LVSIQDRDAVRVITYANVPFGTMTAAGAAEMFDAVAAARMAPSVRVIVITGGLPGIFIRH 62 +V+ + ++ V+T N P T++ ++ D + + + V++ITG F+ Sbjct: 6 VVTCNKKGSIAVVTIQNPPVNTLSLEVVQQLVDVLEEIEIDNDISVVIITGSGEKTFVAG 65 Query: 63 YDVAELSEAADAIERGASRAPSSPGPRPPGFHALTDAIAAVDKPVIAAINGLCMGGGFEL 122 ++ E E+ A + + + + KP IAA+NGL +GGG EL Sbjct: 66 GNIKEFPNWMGKGEKYAEM-------KSLELQQPLNQLENLSKPTIAAVNGLALGGGCEL 118 Query: 123 SLACDLRIAGSSVTAIGLPETRIGIFPGGGGTQRLPRIVGEARALEMILRGLTVTGQRAH 182 +LACDLRI + IGLPE +G+FPG GGTQRLPR++GE +A EM+ G ++T + A Sbjct: 119 ALACDLRIIEEQ-SLIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKSITAKEAK 177 Query: 183 EIGIVHEVV--ADPLARSLEIGAELSGRSAEGLAFAKRLTRSALDRPLAEGLADERKSFS 240 EIG+V+ + + L ++ EI ++S S LA+ K R PL EGL E + F Sbjct: 178 EIGLVNYITPRGEALNKAKEIARDISKFSLPALAYMKLAIREGAAVPLQEGLEIEARYFG 237 Query: 241 TVMRTASAREGLRA 254 V +T +EG++A Sbjct: 238 KVFQTEDVKEGVKA 251