BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0028.p (256 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_996586.1| enoyl-CoA hydratase/isomerase [Verminephrobacte... 182 3e-44 ref|YP_360127.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydother... 180 1e-43 ref|YP_360429.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydother... 180 2e-43 ref|YP_003393465.1| enoyl-CoA hydratase/isomerase [Conexibacter ... 178 8e-43 ref|ZP_05092977.1| enoyl-CoA hydratase/isomerase family protein ... 177 1e-42 >ref|YP_996586.1| enoyl-CoA hydratase/isomerase [Verminephrobacter eiseniae EF01-2] gb|ABM57568.1| short chain enoyl-CoA hydratase [Verminephrobacter eiseniae EF01-2] Length = 268 Score = 182 bits (463), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 112/261 (42%), Positives = 158/261 (60%), Gaps = 14/261 (5%) Query: 4 LGSVNYDVQDNIAILTLDEPNKLNALTSGIRMGI-REGLQRADQDDAVRVSIITGAGGKA 62 L SV Y+ ++N+AI+TL+ P ++N L ++ + R + A D+ VR +ITG+G +A Sbjct: 8 LASVLYERRENVAIVTLNRPGRMNTLGGSMKPDLARAFFEYARADERVRAVLITGSGERA 67 Query: 63 FCAGADIGTFDFDPEKARAFFID---AMEVLSAPERALKPVIAAVNGIAYGGGFELAIAS 119 FCAGADI +F+ E+L E KPV+AA+NG+A GGG E+A+ Sbjct: 68 FCAGADIKERADQQTTGSDYFVAQKATHELLRNIEEFEKPVVAAINGVALGGGLEVALCC 127 Query: 120 DFILAAESARFGVPEIKLGLLPGFA-IVRLQDIVGRAKAKEMSILGDPVDAAEASRLGLV 178 D LA +SARFG+PE+KLG++P RL ++G+A+AKE+ + D +DA A R G+V Sbjct: 128 DIRLACDSARFGLPEVKLGVIPAAGGTQRLPRLIGQARAKELILTADLIDADTALRYGIV 187 Query: 179 SRVVPDERLLVDAIAFAQRIASQPRLAVQMAKSFYNRGL------GGNEMRHAIDGFPFL 232 SRV+P L+ AIAFAQRIA P LAV+ AK NRGL G R+A + Sbjct: 188 SRVLPQAELMPAAIAFAQRIAEHPPLAVRFAKRAINRGLQTDLDSGLEYERYAA---AMV 244 Query: 233 LMHADAREGINAFREKREPRF 253 + AD +EG+ AF EKR+P F Sbjct: 245 IDSADRKEGMRAFVEKRKPVF 265 >ref|YP_360127.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydothermus hydrogenoformans Z-2901] gb|ABB14662.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydothermus hydrogenoformans Z-2901] Length = 257 Score = 180 bits (457), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 107/247 (43%), Positives = 158/247 (63%), Gaps = 5/247 (2%) Query: 12 QDNIAILTLDEPNKLNALTSGIRMGIREGLQRADQDDAVRVSIITGAGGKAFCAGADIGT 71 ++ IAILT++ N LNAL + + + +++ L + +D +++V IITGAG KAF AGADI Sbjct: 9 EEGIAILTINRANVLNALNTEVLLELKKVLSQIKEDTSIKVVIITGAGEKAFVAGADISE 68 Query: 72 F-DFDPEKARAFFIDAMEVLSAPERALKPVIAAVNGIAYGGGFELAIASDFILAAESARF 130 ++ + EVL+ E +PVIAAVNG A GGG ELA+A D +A+E A+F Sbjct: 69 MVKQSVDEGYRYSRLGQEVLALIENLPQPVIAAVNGYALGGGCELAMACDMRIASEKAKF 128 Query: 131 GVPEIKLGLLPGF-AIVRLQDIVGRAKAKEMSILGDPVDAAEASRLGLVSRVVPDERLLV 189 G+PEI LG++PGF RL ++VG+AKA E+ + G+ +DAA+A RLGLV++VV ++LL Sbjct: 129 GLPEINLGIIPGFGGTKRLTELVGKAKAMELILTGEMIDAAQAERLGLVNQVVKADKLLE 188 Query: 190 DAIAFAQRIASQPRLAVQMAKSFYNRGLGGN-EMRHAIDG--FPFLLMHADAREGINAFR 246 A AQ+IAS+ ++AV+ AK N+ L + E +A + F D +EG+ AF Sbjct: 189 TAKTLAQKIASKSQIAVRAAKLAVNKSLYTDIETANACEAGLFGVCFASEDRKEGMMAFL 248 Query: 247 EKREPRF 253 EKR +F Sbjct: 249 EKRPAKF 255 >ref|YP_360429.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydothermus hydrogenoformans Z-2901] gb|ABB15921.1| 3-hydroxybutyryl-CoA dehydratase [Carboxydothermus hydrogenoformans Z-2901] Length = 260 Score = 180 bits (457), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 104/253 (41%), Positives = 156/253 (61%), Gaps = 5/253 (1%) Query: 6 SVNYDVQDNIAILTLDEPNKLNALTSGIRMGIREGLQRADQDDAVRVSIITGAGGKAFCA 65 ++ ++ +D + I+T++ P LNAL S + + L + +D+++ V I+TGAG K+F A Sbjct: 5 TILFEKKDQVGIITINRPQVLNALNSEVLEELDSLLDKIAEDESITVVILTGAGEKSFVA 64 Query: 66 GADIGTF-DFDPEKARAFFIDAMEVLSAPERALKPVIAAVNGIAYGGGFELAIASDFILA 124 GADI +F P +AR F +VLS ER +PVIAAVNG A GGG E+A+A DF +A Sbjct: 65 GADISQMRNFTPRQARYFAKLGQKVLSKLERIPQPVIAAVNGFALGGGCEIAMACDFRIA 124 Query: 125 AESARFGVPEIKLGLLPGF-AIVRLQDIVGRAKAKEMSILGDPVDAAEASRLGLVSRVVP 183 + A+FG PE+ LG+ GF RL +VG+ A E+ G+ +DA EA R+GLV+RVV Sbjct: 125 STKAKFGQPEVGLGVTAGFGGTQRLPRLVGKGMAAELLYTGEMIDAQEALRIGLVNRVVE 184 Query: 184 DERLLVDAIAFAQRIASQPRLAVQMAKSFYNRGLGGNEMR---HAIDGFPFLLMHADARE 240 E L+ A+ AQ+IA++ +LAV +K+ N GL + R + + F +D +E Sbjct: 185 PEELMPTALEIAQKIAAKAKLAVFYSKAALNEGLNMDLERALAYEAEMFALCFTTSDQKE 244 Query: 241 GINAFREKREPRF 253 G++AF KR+P F Sbjct: 245 GMDAFLNKRKPEF 257 >ref|YP_003393465.1| enoyl-CoA hydratase/isomerase [Conexibacter woesei DSM 14684] gb|ADB50090.1| Enoyl-CoA hydratase/isomerase [Conexibacter woesei DSM 14684] Length = 367 Score = 178 bits (451), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 109/251 (43%), Positives = 145/251 (57%), Gaps = 3/251 (1%) Query: 5 GSVNYDVQDNIAILTLDEPNKLNALTSGIRMGIREGLQRADQDDAVRVSIITGAGGKAFC 64 G V + +D IAI LD P N+L+ +R +R + D AVRV + A FC Sbjct: 115 GPVKLETRDAIAIAWLDNPPA-NSLSPTTIAALRSVWERVEDDPAVRVLVFASANPALFC 173 Query: 65 AGADIGTFD-FDPEKARAFFIDAMEVLSAPERALKPVIAAVNGIAYGGGFELAIASDFIL 123 AGADI F D RA +A E+L + ER+ IAAVN +A+GGG ELA+A+D + Sbjct: 174 AGADIKAFAAMDEAGGRALLDEAHELLRSWERSSTVTIAAVNALAFGGGCELAMATDIRI 233 Query: 124 AAESARFGVPEIKLGLLPGF-AIVRLQDIVGRAKAKEMSILGDPVDAAEASRLGLVSRVV 182 AA SA FG PEI LG++PGF RL +VG AKA+EM++ G+P+ A EA GLV+R+V Sbjct: 234 AAASASFGQPEINLGIIPGFGGTQRLPRLVGEAKAREMNLTGEPMSAEEAYEFGLVNRLV 293 Query: 183 PDERLLVDAIAFAQRIASQPRLAVQMAKSFYNRGLGGNEMRHAIDGFPFLLMHADAREGI 242 PD L A+A+A+++A Q LAVQ K + GF + DA+EGI Sbjct: 294 PDHELFDTALAWARKLAGQAPLAVQQIKQVSAVPDIDEGVAEEKKGFAIVFGSEDAKEGI 353 Query: 243 NAFREKREPRF 253 AF +KR PRF Sbjct: 354 GAFLQKRTPRF 364 >ref|ZP_05092977.1| enoyl-CoA hydratase/isomerase family protein [Carboxydibrachium pacificum DSM 12653] gb|EEB75156.1| enoyl-CoA hydratase/isomerase family protein [Carboxydibrachium pacificum DSM 12653] Length = 263 Score = 177 bits (450), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 105/259 (40%), Positives = 153/259 (59%), Gaps = 5/259 (1%) Query: 1 MLRLGSVNYDVQDNIAILTLDEPNKLNALTSGIRMGIREGLQRADQDDAVRVSIITGAGG 60 ++ ++ ++ IA +T++ P LNAL + ++ L+ DD V+V +ITGAG Sbjct: 3 LVEYKNIEVKIEKGIATITINRPKALNALNTETLEELKNVLEVLQNDDGVKVIVITGAGE 62 Query: 61 KAFCAGADIGTF-DFDPEKARAFFIDAMEVLSAPERALKPVIAAVNGIAYGGGFELAIAS 119 KAF AGADI D +A+ F +V E KPVIAAVNG A GGG ELA+A Sbjct: 63 KAFVAGADISEMKDMSVFEAKKFAELGQKVFRKIELMKKPVIAAVNGYALGGGCELALAC 122 Query: 120 DFILAAESARFGVPEIKLGLLPGF-AIVRLQDIVGRAKAKEMSILGDPVDAAEASRLGLV 178 D +A+ +A+FG PE+ LG++PGF RL IVG +KAKE+ GD +DA EA R+GL+ Sbjct: 123 DIRIASRNAKFGQPEVGLGIIPGFGGTQRLPRIVGVSKAKELIYTGDMIDAEEALRIGLI 182 Query: 179 SRVVPDERLLVDAIAFAQRIASQPRLAVQMAKSFYNRGLG---GNEMRHAIDGFPFLLMH 235 S+VV ++LL +A A++I S+ +AV +AK N+ L + M + + F Sbjct: 183 SKVVEQDKLLEEAYGIAKKIMSKGLVAVSLAKEAINKSLEVDIDSGMEYEANAFAMCFGT 242 Query: 236 ADAREGINAFREKREPRFD 254 D +EG+ AF EKR P+F+ Sbjct: 243 QDQKEGMVAFLEKRAPKFE 261