BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0022.p (333 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_01000029.1| putative acid--CoA ligase [Oceanicola batsens... 434 e-119 ref|ZP_02188801.1| citrate synthase [alpha proteobacterium BAL19... 403 e-110 ref|ZP_07375584.1| AMP-dependent synthetase and ligase [Ahrensia... 391 e-107 ref|YP_004127500.1| amp-dependent synthetase and ligase [Alicycl... 360 2e-97 ref|YP_004387539.1| o-succinylbenzoate--CoA ligase [Alicycliphil... 358 9e-97 >ref|ZP_01000029.1| putative acid--CoA ligase [Oceanicola batsensis HTCC2597] gb|EAQ02500.1| putative acid--CoA ligase [Oceanicola batsensis HTCC2597] Length = 506 Score = 434 bits (1115), Expect = e-119, Method: Compositional matrix adjust. Identities = 207/326 (63%), Positives = 255/326 (78%), Gaps = 1/326 (0%) Query: 1 VISHRAMVMRALVFSSELGIAPHDTFVAWAPLFHMASTDHALATLLRGGTVVVIDGFQLE 60 +ISHRA + R++ F+++L + P D F+AWAP+FHMASTDHALAT+LRGGTVV++DG Q Sbjct: 177 LISHRAHIARSMAFAAQLALDPGDGFIAWAPMFHMASTDHALATILRGGTVVMVDGLQPA 236 Query: 61 PLLTAVSQHDIGWLVLIPGMVEALIDGLKAQRTKVKGVRVCGAMADLVAPHAIAEVTEML 120 + A+S++ IGW V++PG ++A I +A VKG++VCGAMADLV PH IAE+T +L Sbjct: 237 VINEALSRYRIGWFVMMPGALDAFIAERRANPLPVKGIKVCGAMADLVPPHQIAELTALL 296 Query: 121 GAPYLNSFGSTETGLPPATRALIAPGDVPASLSKRQNAFCQIKLVDTSDNEVAPGEPGEL 180 PYLNSFG+TETGLPP T LIAPG P LSKR +AFC+++LVD D EV G PGE+ Sbjct: 297 DTPYLNSFGATETGLPPGTADLIAPGVTPDRLSKRISAFCEVRLVDPDDREVRDGTPGEM 356 Query: 181 AIRSPTLFSGYWRADETNARDFRGGWFHMGDVFRRNADGSLDFVDRAKYLIKSGGENIYP 240 A+R PTLFSGYW A++TNARDFR G+FHMGD+FRRNADG++DFVDRAKYLIK+GGEN+YP Sbjct: 357 AVRGPTLFSGYWNAEDTNARDFRNGFFHMGDLFRRNADGTVDFVDRAKYLIKTGGENVYP 416 Query: 241 AEIERVLLGDDRIAEVAVVRAADPRWGEAPVAFVACREGAAMTVAELTELCRRELASYKR 300 AEIERVLL + + AVVRA D +WGE+ VAFVAC +G A L LCR LA YKR Sbjct: 417 AEIERVLLSHPGVVDAAVVRAFDVKWGESSVAFVACSKGGPDAEA-LMNLCRENLAGYKR 475 Query: 301 PREFRFITFGEFPRSTSGKVQRHELE 326 PR+F FI F +FPRSTSGKVQRH LE Sbjct: 476 PRKFLFIAFEDFPRSTSGKVQRHILE 501 >ref|ZP_02188801.1| citrate synthase [alpha proteobacterium BAL199] gb|EDP64629.1| citrate synthase [alpha proteobacterium BAL199] Length = 515 Score = 403 bits (1035), Expect = e-110, Method: Compositional matrix adjust. Identities = 191/329 (58%), Positives = 239/329 (72%), Gaps = 1/329 (0%) Query: 1 VISHRAMVMRALVFSSELGIAPHDTFVAWAPLFHMASTDHALATLLRGGTVVVIDGFQLE 60 VISHRA ++R LV +E G+AP DTF AW+PL+HM + + ++ L+ GG V +DGF + Sbjct: 183 VISHRAEIVRNLVIRAEFGVAPEDTFAAWSPLYHMGAAECSIGALMTGGKVTPVDGFDQD 242 Query: 61 PLLTAVSQHDIGWLVLIPGMVEALIDGLKAQRTKVKGVRVCGAMADLVAPHAIAEVTEML 120 L ++ +GWL+L+PGMV A D L+ + +GV+VCG MADLV P IA +T +L Sbjct: 243 RLAEVIATEPLGWLLLMPGMVGAFADELERRGIVARGVKVCGVMADLVPPADIARITRLL 302 Query: 121 GAPYLNSFGSTETGLPPATRALIAPGDVPASLSKRQNAFCQIKLVDTSDNEVAPGEPGEL 180 APY N+FG+TETG PP + LI G PA LSK+Q+ FC+++LVD D+EV G GEL Sbjct: 303 DAPYANTFGATETGCPPCSSNLIPVGVAPARLSKQQSPFCEVRLVDPDDHEVPDGSAGEL 362 Query: 181 AIRSPTLFSGYWRADETNARDFRGGWFHMGDVFRRNADGSLDFVDRAKYLIKSGGENIYP 240 +R PTLFSGYWRA E NA+DFRGGWFHMGDVF RN DG+LDFVDR KYLIKSGGENIYP Sbjct: 363 CMRGPTLFSGYWRAAEVNAQDFRGGWFHMGDVFVRNPDGTLDFVDRVKYLIKSGGENIYP 422 Query: 241 AEIERVLLGDDRIAEVAVVRAADPRWGEAPVAFVACREGAAMTVAELTELCRRELASYKR 300 AEIERV+L D R+ E AVVR +DP+WGE PVAFVA R ++T +L CR ELA YK+ Sbjct: 423 AEIERVMLADPRVTEAAVVRRSDPKWGEVPVAFVA-RRDESLTADDLHRRCRDELAGYKQ 481 Query: 301 PREFRFITFGEFPRSTSGKVQRHELERLL 329 P+ FI +FPRS SGK+QRHELE+ L Sbjct: 482 PKGIHFIDHDDFPRSASGKIQRHELEKRL 510 >ref|ZP_07375584.1| AMP-dependent synthetase and ligase [Ahrensia sp. R2A130] gb|EFL89035.1| AMP-dependent synthetase and ligase [Ahrensia sp. R2A130] Length = 500 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 190/326 (58%), Positives = 234/326 (71%), Gaps = 4/326 (1%) Query: 1 VISHRAMVMRALVFSSELGIAPHDTFVAWAPLFHMASTDHALATLLRGGTVVVIDGFQLE 60 ++SHRA V RA F S+LG+ P FVAWAP +HMASTDHALATLLRGGTV V+DG+Q Sbjct: 174 LVSHRATVARAAAFVSDLGLEPGANFVAWAPFYHMASTDHALATLLRGGTVFVVDGYQPS 233 Query: 61 PLLTAVSQHDIGWLVLIPGMVEALIDGLKAQRTKVKGVRVCGAMADLVAPHAIAEVTEML 120 L+ ++Q+ IGW V++PGM+ A + KG+ GAMADLV +A++T L Sbjct: 234 LLIEILAQYKIGWFVVMPGMIADFAKQCVAAALQPKGITCIGAMADLVPRDDLAKITHAL 293 Query: 121 GAPYLNSFGSTETGLPPATRALIAPGDVPASLSKRQNAFCQIKLVDTSDNEVAPGEPGEL 180 APYLNSFG+TETGLPPATR+++A GD P L KRQ+AF +I+LVD D +V GE GEL Sbjct: 294 NAPYLNSFGATETGLPPATRSVVAIGDAPDRLPKRQSAFVEIRLVDAEDRDVPIGEAGEL 353 Query: 181 AIRSPTLFSGYWRADETNARDFRGGWFHMGDVFRRNADGSLDFVDRAKYLIKSGGENIYP 240 A+R PTLFSGYW A ETN DFRGGWFHMGDV RRN DG+L++VDR KY+IKSGGENIYP Sbjct: 354 ALRGPTLFSGYWNAKETNVYDFRGGWFHMGDVMRRNKDGTLEYVDRVKYMIKSGGENIYP 413 Query: 241 AEIERVLLGDDRIAEVAVVRAADPRWGEAPVAFVACREGAAMTVAELTELCRRELASYKR 300 AEIE+VLL + + VVR D WGE PVAFV + EL LC+ +L++YK Sbjct: 414 AEIEQVLLTHPDVEQAIVVRKVDKEWGEVPVAFVV----GIASEHELAALCKADLSTYKW 469 Query: 301 PREFRFITFGEFPRSTSGKVQRHELE 326 PR+F F+ E PRST+GK+QRH LE Sbjct: 470 PRKFIFVEDTELPRSTTGKIQRHLLE 495 >ref|YP_004127500.1| amp-dependent synthetase and ligase [Alicycliphilus denitrificans BC] gb|ADV00613.1| AMP-dependent synthetase and ligase [Alicycliphilus denitrificans BC] Length = 509 Score = 360 bits (924), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 175/331 (52%), Positives = 232/331 (70%), Gaps = 2/331 (0%) Query: 1 VISHRAMVMRALVFSSELGIAPHDTFVAWAPLFHMASTDHALATLLRGGTVVVIDGFQLE 60 VIS RA + RA+ ++ E GI D F+AW+P FHMA+TDH+LATL+ GG V+V DGF Sbjct: 179 VISQRASIARAMAYACEYGIGAEDGFIAWSPFFHMAATDHSLATLMLGGHVIVQDGFSAS 238 Query: 61 PLLTAVSQHDIGWLVLIPGMVEALIDGLK-AQRTKVKGVRVCGAMADLVAPHAIAEVTEM 119 + + + IGWL+ +PG++E LI+ LK A + + GAMADLV IAE+T + Sbjct: 239 EICKTLERERIGWLMAMPGVIEPLINALKSASPFLAPRMGMVGAMADLVPCQQIAELTSL 298 Query: 120 LGAPYLNSFGSTETGLPPATRALIAPGDVPASLSKRQNAFCQIKLVDTSDNEVAPGEPGE 179 +GAPYLNSFGSTETGL PA+ AL+ G VP SLSKR++++C ++LVD + +V G PG Sbjct: 299 VGAPYLNSFGSTETGLAPASGALLKAGQVPESLSKRESSWCHVRLVDDDNQDVPIGTPGN 358 Query: 180 LAIRSPTLFSGYWRADETNARDFRGGWFHMGDVFRRNADGSLDFVDRAKYLIKSGGENIY 239 + +R P LFSGYW + + GWFH+GDVF R+ADGSLDFVDR+KYLIK+GGEN+Y Sbjct: 359 MLVRGPGLFSGYWSNGRIESAELADGWFHLGDVFVRHADGSLDFVDRSKYLIKTGGENVY 418 Query: 240 PAEIERVLLGDDRIAEVAVVRAADPRWGEAPVAFVACREGAAMTVAELTELCRRELASYK 299 PAEIERVLL + E AVVR +D RWGE PVAFVA + G + + L CR LA YK Sbjct: 419 PAEIERVLLAHPDVIEAAVVRRSDDRWGEVPVAFVALK-GESRPLDFLQAYCREHLAGYK 477 Query: 300 RPREFRFITFGEFPRSTSGKVQRHELERLLK 330 P+EFR I+ FPR+++GK+Q+ ++E LL+ Sbjct: 478 VPKEFRVISAAAFPRNSTGKIQKRQVEALLR 508 >ref|YP_004387539.1| o-succinylbenzoate--CoA ligase [Alicycliphilus denitrificans K601] gb|AEB84023.1| o-succinylbenzoate--CoA ligase [Alicycliphilus denitrificans K601] Length = 509 Score = 358 bits (918), Expect = 9e-97, Method: Compositional matrix adjust. Identities = 174/331 (52%), Positives = 231/331 (69%), Gaps = 2/331 (0%) Query: 1 VISHRAMVMRALVFSSELGIAPHDTFVAWAPLFHMASTDHALATLLRGGTVVVIDGFQLE 60 VIS RA + RA+ ++ E I D F+AW+P FHMA+TDH+LATL+ GG V+V DGF Sbjct: 179 VISQRASIARAMAYACEYRIGAEDGFIAWSPFFHMAATDHSLATLMLGGHVIVQDGFSAS 238 Query: 61 PLLTAVSQHDIGWLVLIPGMVEALIDGLK-AQRTKVKGVRVCGAMADLVAPHAIAEVTEM 119 + + + IGWL+ +PG++E LI+ LK A + + GAMADLV IAE+T + Sbjct: 239 EICKTLERERIGWLMAMPGVIEPLINALKSASPVLAPRMGMVGAMADLVPCQQIAELTSL 298 Query: 120 LGAPYLNSFGSTETGLPPATRALIAPGDVPASLSKRQNAFCQIKLVDTSDNEVAPGEPGE 179 +GAPYLNSFGSTETGL PA+ AL+ G VP SLSKR++++C ++LVD + +V G PG Sbjct: 299 VGAPYLNSFGSTETGLAPASGALLKAGQVPESLSKRESSWCHVRLVDDDNQDVPIGTPGN 358 Query: 180 LAIRSPTLFSGYWRADETNARDFRGGWFHMGDVFRRNADGSLDFVDRAKYLIKSGGENIY 239 + +R P LFSGYW + + GWFH+GDVF R+ADGSLDFVDR+KYLIK+GGEN+Y Sbjct: 359 MLVRGPGLFSGYWSNGRIESAELADGWFHLGDVFVRHADGSLDFVDRSKYLIKTGGENVY 418 Query: 240 PAEIERVLLGDDRIAEVAVVRAADPRWGEAPVAFVACREGAAMTVAELTELCRRELASYK 299 PAEIERVLL + E AVVR +D RWGE PVAFVA + G + + L CR LA YK Sbjct: 419 PAEIERVLLAHPDVIEAAVVRRSDDRWGEVPVAFVALK-GESRPLDFLQAYCREHLAGYK 477 Query: 300 RPREFRFITFGEFPRSTSGKVQRHELERLLK 330 P+EFR I+ FPR+++GK+Q+ ++E LL+ Sbjct: 478 VPKEFRVISAAAFPRNSTGKIQKRQVEALLR 508