BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0004.p (331 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_531933.1| tRNA-dihydrouridine synthase A [Rhodopseudomona... 566 e-159 ref|NP_770374.1| tRNA-dihydrouridine synthase A [Bradyrhizobium ... 554 e-156 ref|ZP_08629349.1| tRNA dihydrouridine synthase A [Bradyrhizobia... 548 e-154 ref|YP_487006.1| tRNA-dihydrouridine synthase A [Rhodopseudomona... 541 e-152 ref|YP_001206690.1| tRNA-dihydrouridine synthase A [Bradyrhizobi... 541 e-152 >ref|YP_531933.1| tRNA-dihydrouridine synthase A [Rhodopseudomonas palustris BisB18] gb|ABD87614.1| TIM-barrel protein, yjbN family [Rhodopseudomonas palustris BisB18] Length = 330 Score = 566 bits (1459), Expect = e-159, Method: Compositional matrix adjust. Identities = 272/321 (84%), Positives = 294/321 (91%) Query: 4 QDYRFSVAPMMDWTDRHCRVFHRLMSRRARLYTEMLTTGAIIHGDRARLLGFDVSEHPVA 63 Q +RFSVAPMMDWTDRHCRVFHR +SRRARLY+EMLTTGA+IHGDR RLLGFD SEHP+A Sbjct: 5 QSFRFSVAPMMDWTDRHCRVFHRRLSRRARLYSEMLTTGAVIHGDRQRLLGFDASEHPLA 64 Query: 64 LQLGGSDPRDLATAAQIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGVAAM 123 LQLGGSD DLATAA+IGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEG+AAM Sbjct: 65 LQLGGSDSADLATAAKIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGIAAM 124 Query: 124 KRAVRIPVTVKCRIGIDDQDPEVALDTLARSVIAAGADALVVHARKAWLNGLSPKENRDI 183 KRAV +PVTVKCRIGIDDQDPE+ALD+LAR+V+AAGAD LVVHARKAWLNGLSPKENRD+ Sbjct: 125 KRAVSVPVTVKCRIGIDDQDPELALDSLARAVVAAGADLLVVHARKAWLNGLSPKENRDV 184 Query: 184 PPLDYDRVYRLKAALPDVPIIINGGITGLAEAKRHLAHVDGVMLGRAAYQEPWRLLTVDS 243 PPLDYDRVYRLKAALP VPI+INGGI G+AEAK HL HVDGVMLGRAAYQEPWRLL VD Sbjct: 185 PPLDYDRVYRLKAALPAVPIVINGGIAGIAEAKPHLGHVDGVMLGRAAYQEPWRLLAVDP 244 Query: 244 ELFGETPPHATMKDVFAAMLPYIERQLAEGTRLHSITRHFVGAFHGVPGARAFRRYLAEN 303 ELFGE P+ +M AM PYIE QLA+GTRLHSITRHFVGA+HGVPGARAFRR+LAE Sbjct: 245 ELFGEPAPYPSMTAALQAMTPYIEAQLAQGTRLHSITRHFVGAYHGVPGARAFRRHLAEQ 304 Query: 304 GVRPGADIDVLREAIARVDDR 324 GV+P A IDVLREAIARV+DR Sbjct: 305 GVKPDAGIDVLREAIARVEDR 325 >ref|NP_770374.1| tRNA-dihydrouridine synthase A [Bradyrhizobium japonicum USDA 110] dbj|BAC48999.1| blr3734 [Bradyrhizobium japonicum USDA 110] Length = 331 Score = 554 bits (1427), Expect = e-156, Method: Compositional matrix adjust. Identities = 268/325 (82%), Positives = 293/325 (90%) Query: 7 RFSVAPMMDWTDRHCRVFHRLMSRRARLYTEMLTTGAIIHGDRARLLGFDVSEHPVALQL 66 +F+VAPMMDWTDRHCRVFHR ++RRA LYTEMLTTGAIIHGDR RLLGFD E+PVALQL Sbjct: 7 KFAVAPMMDWTDRHCRVFHRQLTRRALLYTEMLTTGAIIHGDRERLLGFDACENPVALQL 66 Query: 67 GGSDPRDLATAAQIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGVAAMKRA 126 GGSDP +LA AA+IGE FGYDEINLNVGCPSDRVKDGRFGACLMAEP LVA V AMKRA Sbjct: 67 GGSDPSELAQAARIGEAFGYDEINLNVGCPSDRVKDGRFGACLMAEPELVARCVDAMKRA 126 Query: 127 VRIPVTVKCRIGIDDQDPEVALDTLARSVIAAGADALVVHARKAWLNGLSPKENRDIPPL 186 V +PVTVKCRIGIDDQDPEVALDTLAR+V+AAG DAL+VHARKAWLNGLSPKENRDIPPL Sbjct: 127 VAVPVTVKCRIGIDDQDPEVALDTLARAVVAAGCDALIVHARKAWLNGLSPKENRDIPPL 186 Query: 187 DYDRVYRLKAALPDVPIIINGGITGLAEAKRHLAHVDGVMLGRAAYQEPWRLLTVDSELF 246 DY+RVYRLK A+PDVP+IINGGI G+ EAK HL HVDGVMLGRAAYQEPWRLL+VDS++F Sbjct: 187 DYERVYRLKRAMPDVPVIINGGIPGIDEAKAHLEHVDGVMLGRAAYQEPWRLLSVDSDIF 246 Query: 247 GETPPHATMKDVFAAMLPYIERQLAEGTRLHSITRHFVGAFHGVPGARAFRRYLAENGVR 306 GET PHATM+D AM+PYIE QLA GTRLH+ITRHFVGAFH VPGARAFRR+LAE GV+ Sbjct: 247 GETAPHATMQDALEAMMPYIEAQLARGTRLHAITRHFVGAFHAVPGARAFRRHLAEQGVK 306 Query: 307 PGADIDVLREAIARVDDRAPAAVAA 331 PGA +DVLR+AIARV RAPA AA Sbjct: 307 PGAGLDVLRDAIARVGVRAPAEAAA 331 >ref|ZP_08629349.1| tRNA dihydrouridine synthase A [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07842.1| tRNA dihydrouridine synthase A [Bradyrhizobiaceae bacterium SG-6C] Length = 330 Score = 548 bits (1412), Expect = e-154, Method: Compositional matrix adjust. Identities = 269/331 (81%), Positives = 293/331 (88%), Gaps = 1/331 (0%) Query: 1 LKYQDYRFSVAPMMDWTDRHCRVFHRLMSRRARLYTEMLTTGAIIHGDRARLLGFDVSEH 60 +KY ++ FSVAPMMDWTDRHCRVFHRL+SRRARLYTEMLTTGA+IHGDR RLLGFD SEH Sbjct: 1 MKY-NHIFSVAPMMDWTDRHCRVFHRLLSRRARLYTEMLTTGAVIHGDRQRLLGFDASEH 59 Query: 61 PVALQLGGSDPRDLATAAQIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGV 120 PVALQLGGS+PRDLA +A+IGE FGYDEINLNVGCPSDRVKDGRFGACLMAEP LVA+ V Sbjct: 60 PVALQLGGSNPRDLAESARIGEGFGYDEINLNVGCPSDRVKDGRFGACLMAEPRLVADCV 119 Query: 121 AAMKRAVRIPVTVKCRIGIDDQDPEVALDTLARSVIAAGADALVVHARKAWLNGLSPKEN 180 AAMK+AV+IPVTVKCRIGIDDQDPEVALDTL R+VIAAG+DALVVHARKAWLNGLSPKEN Sbjct: 120 AAMKQAVKIPVTVKCRIGIDDQDPEVALDTLGRAVIAAGSDALVVHARKAWLNGLSPKEN 179 Query: 181 RDIPPLDYDRVYRLKAALPDVPIIINGGITGLAEAKRHLAHVDGVMLGRAAYQEPWRLLT 240 RDIPPLDYDRVYRLKAALP VP+IINGGI +A AK HLAHVDG MLGRAAY EPWRLL+ Sbjct: 180 RDIPPLDYDRVYRLKAALPHVPVIINGGIPTVAAAKEHLAHVDGAMLGRAAYHEPWRLLS 239 Query: 241 VDSELFGETPPHATMKDVFAAMLPYIERQLAEGTRLHSITRHFVGAFHGVPGARAFRRYL 300 VD ELFGE PHATM + A++PYIERQLA G RLHSITRH VGAFHGVPGARAFRRYL Sbjct: 240 VDPELFGEPAPHATMHEAIEALMPYIERQLAAGARLHSITRHLVGAFHGVPGARAFRRYL 299 Query: 301 AENGVRPGADIDVLREAIARVDDRAPAAVAA 331 AEN V+ GA + LR A+ VD A++AA Sbjct: 300 AENCVKVGAGTESLRAALDLVDPLTSASIAA 330 >ref|YP_487006.1| tRNA-dihydrouridine synthase A [Rhodopseudomonas palustris HaA2] gb|ABD08095.1| TIM-barrel protein, yjbN family [Rhodopseudomonas palustris HaA2] Length = 332 Score = 541 bits (1395), Expect = e-152, Method: Compositional matrix adjust. Identities = 257/327 (78%), Positives = 287/327 (87%) Query: 2 KYQDYRFSVAPMMDWTDRHCRVFHRLMSRRARLYTEMLTTGAIIHGDRARLLGFDVSEHP 61 + + +RF APMM+WTD CRVFHRL+++RARLYTEM+TTGA+IHGDR RLLGFD SEHP Sbjct: 5 QIRSFRFCTAPMMEWTDSRCRVFHRLLTKRARLYTEMVTTGAVIHGDRQRLLGFDASEHP 64 Query: 62 VALQLGGSDPRDLATAAQIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGVA 121 VALQLGGSDP LATAA+IGEDFGYDEINLNVGCPSDRVK+GRFGACLMAEP LVA+GVA Sbjct: 65 VALQLGGSDPAALATAAKIGEDFGYDEINLNVGCPSDRVKEGRFGACLMAEPELVADGVA 124 Query: 122 AMKRAVRIPVTVKCRIGIDDQDPEVALDTLARSVIAAGADALVVHARKAWLNGLSPKENR 181 AMKRAV IPVTVKCRIGIDDQDPE ALDTLAR V+AAG DAL+VHARKAWLNGLSPKENR Sbjct: 125 AMKRAVEIPVTVKCRIGIDDQDPEAALDTLARGVVAAGVDALIVHARKAWLNGLSPKENR 184 Query: 182 DIPPLDYDRVYRLKAALPDVPIIINGGITGLAEAKRHLAHVDGVMLGRAAYQEPWRLLTV 241 +PPLDYDRVYRLK ALPD+PI+INGGI +AEAK HLAHVDGVMLGRAAYQEPWRLL V Sbjct: 185 HVPPLDYDRVYRLKRALPDLPIVINGGIGSIAEAKLHLAHVDGVMLGRAAYQEPWRLLEV 244 Query: 242 DSELFGETPPHATMKDVFAAMLPYIERQLAEGTRLHSITRHFVGAFHGVPGARAFRRYLA 301 D ELFGE P+ATMKD A++PYIE QLA+G RLH+ITRH +GA+ GVPGARAFRRYL+ Sbjct: 245 DPELFGEAAPYATMKDALEALMPYIEDQLAQGVRLHAITRHLIGAYQGVPGARAFRRYLS 304 Query: 302 ENGVRPGADIDVLREAIARVDDRAPAA 328 E GV+P AD VLR+A+ V+ RA A Sbjct: 305 EFGVKPDADASVLRKAMVAVEQRAAEA 331 >ref|YP_001206690.1| tRNA-dihydrouridine synthase A [Bradyrhizobium sp. ORS 278] emb|CAL78473.1| tRNA-dihydrouridine synthase A [Bradyrhizobium sp. ORS 278] Length = 339 Score = 541 bits (1393), Expect = e-152, Method: Compositional matrix adjust. Identities = 263/330 (79%), Positives = 290/330 (87%) Query: 2 KYQDYRFSVAPMMDWTDRHCRVFHRLMSRRARLYTEMLTTGAIIHGDRARLLGFDVSEHP 61 K +RF+VAPMMDWTDRHCRVFHRL+SRRA LYTEM+TTGA+IHGDR RLLGFD SEHP Sbjct: 10 KSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRLLGFDRSEHP 69 Query: 62 VALQLGGSDPRDLATAAQIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAEGVA 121 VALQLGGSDP DLAT+A+IGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVA VA Sbjct: 70 VALQLGGSDPADLATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAACVA 129 Query: 122 AMKRAVRIPVTVKCRIGIDDQDPEVALDTLARSVIAAGADALVVHARKAWLNGLSPKENR 181 AMKR V IPVTVKCRIGIDDQDPEVALD LA +V+ AGADAL+VHARKAWLNGLSPKENR Sbjct: 130 AMKRVVTIPVTVKCRIGIDDQDPEVALDALADAVVDAGADALIVHARKAWLNGLSPKENR 189 Query: 182 DIPPLDYDRVYRLKAALPDVPIIINGGITGLAEAKRHLAHVDGVMLGRAAYQEPWRLLTV 241 DIPPLDYDRVYRLKA LP VP+IINGGIT + A+ HLAHVDGVMLGRAAYQEPWRLL V Sbjct: 190 DIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAYQEPWRLLDV 249 Query: 242 DSELFGETPPHATMKDVFAAMLPYIERQLAEGTRLHSITRHFVGAFHGVPGARAFRRYLA 301 D LFGE P +MKD AA+ PYIERQ+A+G RLH+ITRH VGA+H VPGARAFRR+LA Sbjct: 250 DPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPGARAFRRHLA 309 Query: 302 ENGVRPGADIDVLREAIARVDDRAPAAVAA 331 E GV+P A I+VLREA+A +++RAP+ A Sbjct: 310 ERGVKPDAGIEVLREAVAMIEERAPSVALA 339