BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0001.p (311 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_01000427.1| Mandelate racemase/muconate lactonizing enzym... 389 e-106 ref|ZP_01747464.1| Mandelate racemase/muconate lactonizing enzym... 369 e-100 ref|ZP_05081142.1| muconate cycloisomerase 1 [Rhodobacterales ba... 353 1e-95 ref|YP_002497651.1| muconate and chloromuconate cycloisomerase [... 303 2e-80 ref|YP_001768279.1| muconate and chloromuconate cycloisomerase [... 280 2e-73 >ref|ZP_01000427.1| Mandelate racemase/muconate lactonizing enzyme [Oceanicola batsensis HTCC2597] gb|EAQ02358.1| Mandelate racemase/muconate lactonizing enzyme [Oceanicola batsensis HTCC2597] Length = 390 Score = 389 bits (998), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/310 (60%), Positives = 236/310 (76%) Query: 1 VESIKAAVTNYLAPALLGQPALAFEAHALRMSKAATRNFAAKGAIESALLDAAGKTLGLP 60 VE+I+A + Y+AP L+GQPALAFEA+A+RM KA +RN +A+ A+ESAL DAAGKTL LP Sbjct: 80 VEAIRANIDTYIAPVLIGQPALAFEANAMRMKKAVSRNSSARAAVESALYDAAGKTLDLP 139 Query: 61 ASALLGGAVRERMEVIWALASGDSGQEVEEAKEKLRRREHRQFKIKFGFNRPQADLKRLQ 120 A+A LGG VR+R EV+WALASGD+ QE+EEA+ KL REHR+FKIK GF+ P D+ RL+ Sbjct: 140 AAAFLGGQVRDRTEVLWALASGDAEQEIEEARAKLAAREHRRFKIKLGFHSPAEDMVRLR 199 Query: 121 ALRAGLGDDVRLIVDVNQGWTEAECIRFMPALEELDVALIEQPVSALQLEAMARVAARTS 180 + + + D ++VDVNQGW+EA IR++P L EL VALIEQP+ QL A AR+AAR++ Sbjct: 200 RIMSAMPDGTEMVVDVNQGWSEATAIRYLPELAELGVALIEQPLHPGQLAATARIAARSA 259 Query: 181 IPLLVDEAAFTKEDIARAGAMGCGSVYSLKLVKSGGLFEMKRAAAVAAAHGFELYGGCLL 240 IP++ DEAAFT +++ + GSVYSLKLVKSGGL E+KRAA +A A G ELYGGCLL Sbjct: 260 IPIMTDEAAFTNQELVQNATAASGSVYSLKLVKSGGLQELKRAAGIAGAFGMELYGGCLL 319 Query: 241 ESSIGAAAHLAAFATLPRLEWGTEHFGPRILVEDLVVNPIRFDEFEICAPDGPGLGVEVD 300 ES IGAAAH+A F+TLP LEWG EHFGPRIL DL + +++F I P GPGLG+ VD Sbjct: 320 ESGIGAAAHMAVFSTLPTLEWGCEHFGPRILKTDLTSAGLVYEDFHILMPSGPGLGIAVD 379 Query: 301 EDKIRAFARK 310 D +R RK Sbjct: 380 RDVLRDATRK 389 >ref|ZP_01747464.1| Mandelate racemase/muconate lactonizing enzyme [Sagittula stellata E-37] gb|EBA06886.1| Mandelate racemase/muconate lactonizing enzyme [Sagittula stellata E-37] Length = 390 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 185/310 (59%), Positives = 228/310 (73%) Query: 1 VESIKAAVTNYLAPALLGQPALAFEAHALRMSKAATRNFAAKGAIESALLDAAGKTLGLP 60 VE+IKA + YLAPALL AL FE +AL+M KA +RN +A+ A+E AL DAAGK L LP Sbjct: 80 VEAIKANIDTYLAPALLDGSALDFELNALKMKKAVSRNTSARAAVEGALYDAAGKALDLP 139 Query: 61 ASALLGGAVRERMEVIWALASGDSGQEVEEAKEKLRRREHRQFKIKFGFNRPQADLKRLQ 120 A+A LGGAVR+R+EV+WALASGD+ QE+EEAK KL R HR+FKIK GF+ P+ D+ RL+ Sbjct: 140 ATAFLGGAVRDRIEVLWALASGDAEQEIEEAKAKLDARLHRRFKIKLGFHEPREDMIRLR 199 Query: 121 ALRAGLGDDVRLIVDVNQGWTEAECIRFMPALEELDVALIEQPVSALQLEAMARVAARTS 180 + + + ++VD+NQGW+EA IR++P L E+ +ALIEQP+ L A ARVA R+ Sbjct: 200 RIMSAMPAGTEMVVDINQGWSEATFIRYVPELAEMGIALIEQPLKPGLLHATARVAQRSP 259 Query: 181 IPLLVDEAAFTKEDIARAGAMGCGSVYSLKLVKSGGLFEMKRAAAVAAAHGFELYGGCLL 240 IP++ DEAAF+ +D+ + GSVYSLKLVKSGGL EMKRAAAVA A G ELYGGCLL Sbjct: 260 IPIMTDEAAFSTQDLVQNAVAATGSVYSLKLVKSGGLQEMKRAAAVAGAFGLELYGGCLL 319 Query: 241 ESSIGAAAHLAAFATLPRLEWGTEHFGPRILVEDLVVNPIRFDEFEICAPDGPGLGVEVD 300 ES IGAAAHLA FATLP LEWG EHFGP IL DL + I + +F I P GPGLG+ VD Sbjct: 320 ESGIGAAAHLAVFATLPALEWGCEHFGPSILQRDLTASGIAYRDFHIETPKGPGLGITVD 379 Query: 301 EDKIRAFARK 310 D + A RK Sbjct: 380 TDALDAATRK 389 >ref|ZP_05081142.1| muconate cycloisomerase 1 [Rhodobacterales bacterium Y4I] gb|EDZ44658.1| muconate cycloisomerase 1 [Rhodobacterales bacterium Y4I] Length = 392 Score = 353 bits (907), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 191/305 (62%), Positives = 234/305 (76%) Query: 1 VESIKAAVTNYLAPALLGQPALAFEAHALRMSKAATRNFAAKGAIESALLDAAGKTLGLP 60 VESI++ +T YLAPAL G PA A A RM++AA RN +AKGAIE+A+ DA +TLG+P Sbjct: 82 VESIQSVITRYLAPALAGCPAARPAAVAARMARAAFRNNSAKGAIEAAVFDALARTLGVP 141 Query: 61 ASALLGGAVRERMEVIWALASGDSGQEVEEAKEKLRRREHRQFKIKFGFNRPQADLKRLQ 120 A+ GGAVR+R+ V+WALASGD+ QE EEA+ KL REHR FKIK GF+ P+ADL+RLQ Sbjct: 142 AATFFGGAVRDRVPVLWALASGDADQEAEEARAKLAAREHRDFKIKLGFDSPEADLRRLQ 201 Query: 121 ALRAGLGDDVRLIVDVNQGWTEAECIRFMPALEELDVALIEQPVSALQLEAMARVAARTS 180 LR + RLIVDVNQ W+EA+C R++PAL+EL V LIEQP+ E MARVAAR+ Sbjct: 202 KLREEVPGMRRLIVDVNQAWSEADCRRYLPALQELQVDLIEQPLPGDDFEGMARVAARSP 261 Query: 181 IPLLVDEAAFTKEDIARAGAMGCGSVYSLKLVKSGGLFEMKRAAAVAAAHGFELYGGCLL 240 IPL+VDEAAFT +I RAG MGCGSVYSLKLVKSGGL + +AA VA + G ELYGGCLL Sbjct: 262 IPLMVDEAAFTHREITRAGTMGCGSVYSLKLVKSGGLMALSQAARVAESSGMELYGGCLL 321 Query: 241 ESSIGAAAHLAAFATLPRLEWGTEHFGPRILVEDLVVNPIRFDEFEICAPDGPGLGVEVD 300 E SIGAAAHLA FA LPRL WG EHFGPR+ V ++V +P+ ++F + P GPGLGV +D Sbjct: 322 EGSIGAAAHLAVFAALPRLPWGCEHFGPRLHVSEIVRDPLVIEDFHLRVPQGPGLGVHLD 381 Query: 301 EDKIR 305 ED++R Sbjct: 382 EDRLR 386 >ref|YP_002497651.1| muconate and chloromuconate cycloisomerase [Methylobacterium nodulans ORS 2060] gb|ACL57348.1| muconate and chloromuconate cycloisomerase [Methylobacterium nodulans ORS 2060] Length = 389 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 175/311 (56%), Positives = 203/311 (65%), Gaps = 1/311 (0%) Query: 1 VESIKAAVTNYLAPALLGQPALAFEAHALRMSKAATRNFAAKGAIESALLDAAGKTLGLP 60 VE IKA + YLAPAL+GQ A F L + AA RN AAK AIE+AL DA GKTLGLP Sbjct: 79 VEGIKATIDAYLAPALIGQRADHFVTAGLVLDAAAKRNNAAKAAIETALFDAVGKTLGLP 138 Query: 61 ASALLGGAVRERMEVIWALASGDSGQEVEEAKEKLRRREHRQFKIKFGFNRPQADLKRLQ 120 A+ALLGGAVR + V+W LASGD QE+ EA KL R HR FKIK G P+ADL RL Sbjct: 139 AAALLGGAVRSSLPVLWTLASGDPDQEIAEAHAKLDARLHRTFKIKIGAQAPEADLARLT 198 Query: 121 ALRAGLGDDVRLIVDVNQGWTEAECIRFMPALEELDVALIEQPVSALQLEAMARVAAR-T 179 L A L D LIVD NQ W E RF+P L +L +AL+EQP+ A + M R+ R Sbjct: 199 RLAAALADRATLIVDANQAWDETTARRFLPRLRDLKIALVEQPLPAWNIAGMGRLRTRDD 258 Query: 180 SIPLLVDEAAFTKEDIARAGAMGCGSVYSLKLVKSGGLFEMKRAAAVAAAHGFELYGGCL 239 S PLL DE FT D+ SLKLVK GGL +++ AAVA A G LYGGCL Sbjct: 259 SPPLLADECVFTAHDMLAVAQGAAADAVSLKLVKHGGLIGLRKVAAVAEAAGIGLYGGCL 318 Query: 240 LESSIGAAAHLAAFATLPRLEWGTEHFGPRILVEDLVVNPIRFDEFEICAPDGPGLGVEV 299 LESSIGAAAHL AFA L L WG EHFGP+IL +DLV P+RF +FE+ P GPGLGV + Sbjct: 319 LESSIGAAAHLQAFAGLRDLAWGCEHFGPQILTDDLVTEPLRFHDFEVHLPAGPGLGVTL 378 Query: 300 DEDKIRAFARK 310 D DK+R +AR Sbjct: 379 DPDKLRHYARS 389 >ref|YP_001768279.1| muconate and chloromuconate cycloisomerase [Methylobacterium sp. 4-46] gb|ACA15845.1| muconate and chloromuconate cycloisomerase [Methylobacterium sp. 4-46] Length = 389 Score = 280 bits (717), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 170/311 (54%), Positives = 202/311 (64%), Gaps = 1/311 (0%) Query: 1 VESIKAAVTNYLAPALLGQPALAFEAHALRMSKAATRNFAAKGAIESALLDAAGKTLGLP 60 VE IKA + YLAP+LLG PA F A R+ AA RN AAK AIE+AL DA G+TLGLP Sbjct: 79 VEGIKATIDRYLAPSLLGHPADRFVAAGERLDAAAKRNNAAKAAIETALFDAVGRTLGLP 138 Query: 61 ASALLGGAVRERMEVIWALASGDSGQEVEEAKEKLRRREHRQFKIKFGFNRPQADLKRLQ 120 +ALLGGAVR + V+W LASGD QE+ EA+ KL R HR FK+K G P+ADL RL Sbjct: 139 VAALLGGAVRASLPVLWTLASGDPDQEIAEAEAKLDARLHRTFKVKIGAQAPEADLARLA 198 Query: 121 ALRAGLGDDVRLIVDVNQGWTEAECIRFMPALEELDVALIEQPVSALQLEAMARVAARTS 180 L L LIVD NQ W E R +P L +L +AL+EQP+ A + M R+ AR Sbjct: 199 RLARALAGRATLIVDANQAWDETTARRVLPRLADLGIALVEQPLPAWNVAGMGRLRARDD 258 Query: 181 I-PLLVDEAAFTKEDIARAGAMGCGSVYSLKLVKSGGLFEMKRAAAVAAAHGFELYGGCL 239 + PLL DE FT D+ SLKLVK GGL +++ AAVA A G LYGGCL Sbjct: 259 VPPLLADECVFTSHDMLAVAQAAAADAVSLKLVKHGGLVGLRKVAAVAEAAGIALYGGCL 318 Query: 240 LESSIGAAAHLAAFATLPRLEWGTEHFGPRILVEDLVVNPIRFDEFEICAPDGPGLGVEV 299 LESSIGAAAHL AFA L L WG EHFGP+IL +DLV P+RF +F + PDGPGLGV + Sbjct: 319 LESSIGAAAHLQAFAGLRDLAWGCEHFGPQILTDDLVTEPLRFHDFAVHLPDGPGLGVAL 378 Query: 300 DEDKIRAFARK 310 D DK+R +AR Sbjct: 379 DPDKLRHYARS 389