BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0129.0061.p (346 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_766750.1| gentisate 1,2-dioxygenase [Bradyrhizobium japon... 659 0.0 ref|YP_001243259.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp... 630 e-178 ref|YP_001202852.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp... 621 e-176 ref|YP_004305827.1| gentisate 1,2-dioxygenase with a Cupin, RmlC... 473 e-131 dbj|BAC98955.1| gentisate 1,2-dioxygenase [Xanthobacter polyarom... 458 e-127 >ref|NP_766750.1| gentisate 1,2-dioxygenase [Bradyrhizobium japonicum USDA 110] dbj|BAC45375.1| gentisate 1,2-dioxygenase [Bradyrhizobium japonicum USDA 110] Length = 346 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/346 (89%), Positives = 327/346 (94%) Query: 1 MEAVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGK 60 MEAV KTPEREAFYKKIDG+NL+ALW VM DLITPEPKSACRPHLWKFD IRDYM EAGK Sbjct: 1 MEAVTKTPEREAFYKKIDGENLTALWTVMGDLITPEPKSACRPHLWKFDVIRDYMREAGK 60 Query: 61 LITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKG 120 LITAKEAERRVLVLENPGLRGQSKITTSL+AGVQMVVPGD+APAHRH+QSALRFVLEGKG Sbjct: 61 LITAKEAERRVLVLENPGLRGQSKITTSLYAGVQMVVPGDVAPAHRHSQSALRFVLEGKG 120 Query: 121 AFTAVDGERTAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKE 180 A TAVDGERTAM PGDF+ITPSMTWHDHSNET+EPMFWLDGLDIP+VQFFDCSFAEG+KE Sbjct: 121 AHTAVDGERTAMEPGDFIITPSMTWHDHSNETDEPMFWLDGLDIPLVQFFDCSFAEGSKE 180 Query: 181 DQQSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWDACHG 240 DQQ I+KPAG SFARYGHNLLP+D KR+SKTSPIF+YPY YTREALEKA+ EWDACHG Sbjct: 181 DQQRITKPAGDSFARYGHNLLPVDVKRSSKTSPIFSYPYAYTREALEKARASQEWDACHG 240 Query: 241 LKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEW 300 LKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYR+TDATVF IEG+GR+R+GD FEW Sbjct: 241 LKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRSTDATVFCPIEGRGRSRIGDAVFEW 300 Query: 301 GPRDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDRGNA 346 GPRDLFVVPSWHWVTHEAD D+VLFSFSDRPVQQKLDLFREDRGNA Sbjct: 301 GPRDLFVVPSWHWVTHEADDDAVLFSFSDRPVQQKLDLFREDRGNA 346 >ref|YP_001243259.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp. BTAi1] gb|ABQ39353.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp. BTAi1] Length = 345 Score = 630 bits (1624), Expect = e-178, Method: Compositional matrix adjust. Identities = 290/344 (84%), Positives = 317/344 (92%) Query: 3 AVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKLI 62 A TPEREAFYKKIDG N++ALW VM+DLITPEPKSACRPHLW+F IRDYM EAG+LI Sbjct: 2 AAVSTPEREAFYKKIDGANMTALWTVMSDLITPEPKSACRPHLWQFAAIRDYMIEAGRLI 61 Query: 63 TAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGAF 122 TAKEAERRVL+LENPGLRGQSKITTSL+AG+QMV+PGDIAPAHRH+QSALRFVLEG+GAF Sbjct: 62 TAKEAERRVLILENPGLRGQSKITTSLYAGIQMVIPGDIAPAHRHSQSALRFVLEGQGAF 121 Query: 123 TAVDGERTAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKEDQ 182 T VDGERT M PGDFVITPSMTWHDH NET+EPMFWLDGLDIP+VQF D SFA+G+ EDQ Sbjct: 122 TTVDGERTLMQPGDFVITPSMTWHDHGNETSEPMFWLDGLDIPLVQFLDASFAQGSAEDQ 181 Query: 183 QSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWDACHGLK 242 Q +++PAG SFARYGHNLLP+D KR S+TSPIFNYPY YTREALE AK +EWDACHGLK Sbjct: 182 QKVARPAGDSFARYGHNLLPVDAKRRSRTSPIFNYPYAYTREALESAKRREEWDACHGLK 241 Query: 243 LKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWGP 302 LKFSNPETGDFAMPTIGTFIQLLPKGFKTARYR+TDATVF AIEGKGR+R+GD TFEWGP Sbjct: 242 LKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRSTDATVFTAIEGKGRSRIGDHTFEWGP 301 Query: 303 RDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDRGNA 346 RDLFVVPSW WV+HEAD D+VLFSFSDRPVQ+KLDLFREDRGNA Sbjct: 302 RDLFVVPSWQWVSHEADEDAVLFSFSDRPVQEKLDLFREDRGNA 345 >ref|YP_001202852.1| gentisate 1,2-dioxygenase [Bradyrhizobium sp. ORS 278] emb|CAL74612.1| Putative gentisate 1,2-dioxygenase with a Cupin, RmlC-type domain [Bradyrhizobium sp. ORS 278] Length = 345 Score = 621 bits (1602), Expect = e-176, Method: Compositional matrix adjust. Identities = 285/344 (82%), Positives = 314/344 (91%) Query: 3 AVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKLI 62 A TPEREAFYKKID +N++ALW VM+DLITPEPKS CRPHLW+F IRDYM EAG+LI Sbjct: 2 AAVSTPEREAFYKKIDRENMTALWTVMSDLITPEPKSGCRPHLWQFAAIRDYMIEAGRLI 61 Query: 63 TAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGAF 122 TAKEAERRVL+LENPGLRGQSKITTSL+AG+QMV+PGDIAPAHRH+QSALRFVLEG+GAF Sbjct: 62 TAKEAERRVLILENPGLRGQSKITTSLYAGIQMVIPGDIAPAHRHSQSALRFVLEGQGAF 121 Query: 123 TAVDGERTAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKEDQ 182 T VDGERT M PGDF+ITPSMTWHDH NET EPMFWLDGLDIP+VQF D SFA+G++EDQ Sbjct: 122 TTVDGERTLMQPGDFIITPSMTWHDHGNETAEPMFWLDGLDIPLVQFLDASFAQGSQEDQ 181 Query: 183 QSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWDACHGLK 242 Q +++PAG SFARYGHNLLP+D KR S+TSPIFNYPY YTREALE AK EWDACHGLK Sbjct: 182 QKVARPAGDSFARYGHNLLPVDAKRKSRTSPIFNYPYAYTREALENAKRSGEWDACHGLK 241 Query: 243 LKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWGP 302 LKFSNPETGDFAMPTIGTFIQLLPKGF+TARYR+TDATVF AIEG+GR+R+GD FEWGP Sbjct: 242 LKFSNPETGDFAMPTIGTFIQLLPKGFETARYRSTDATVFTAIEGRGRSRIGDAIFEWGP 301 Query: 303 RDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDRGNA 346 RDLFVVPSW WVTHEAD D+VLFSFSDRPVQ+KLDLFREDRGNA Sbjct: 302 RDLFVVPSWQWVTHEADQDAVLFSFSDRPVQEKLDLFREDRGNA 345 >ref|YP_004305827.1| gentisate 1,2-dioxygenase with a Cupin, RmlC-type domain [Polymorphum gilvum SL003B-26A1] gb|ADZ72521.1| Putative gentisate 1,2-dioxygenase with a Cupin, RmlC-type domain [Polymorphum gilvum SL003B-26A1] Length = 352 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 217/346 (62%), Positives = 275/346 (79%) Query: 1 MEAVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGK 60 +E +TPER FY+KID + +ALW V++D+ITPEP+S C+ HLW FD+ + Y+ EAG Sbjct: 4 IEKPVETPERREFYEKIDSSSYTALWTVLSDIITPEPRSTCQSHLWHFDEAKAYLLEAGN 63 Query: 61 LITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKG 120 LITAKEAERRVLVLENPGLRGQS+ITTSL+AG+Q+V+PG++APAHRH+QSALRFV++G G Sbjct: 64 LITAKEAERRVLVLENPGLRGQSRITTSLYAGLQIVMPGEVAPAHRHSQSALRFVMDGSG 123 Query: 121 AFTAVDGERTAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKE 180 A T+VDGERT M GDFVITP WHDH N++++PM WLDGLDIP+V FD SFAE E Sbjct: 124 AHTSVDGERTIMQFGDFVITPPGAWHDHGNDSDQPMVWLDGLDIPIVSVFDASFAEEHHE 183 Query: 181 DQQSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWDACHG 240 DQQ I++ S R+G N+LP+D +RT +SPIFNYPYE +REAL + E D CHG Sbjct: 184 DQQPITRQVEDSSWRFGANMLPVDYERTRLSSPIFNYPYERSREALAHLERQGELDPCHG 243 Query: 241 LKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEW 300 +K+++ NP G +AMPTI +QLLP+GFK+ RYR+TDATVF EG+GR+ + +T+EW Sbjct: 244 IKMRYVNPVDGGWAMPTISPCLQLLPRGFKSQRYRSTDATVFVVTEGRGRSTIAGKTYEW 303 Query: 301 GPRDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDRGNA 346 GPRD+FVVPSW VTHEAD+D+VLFS+SDR Q+KL L+REDRGNA Sbjct: 304 GPRDIFVVPSWKAVTHEADSDAVLFSYSDRVCQEKLGLWREDRGNA 349 >dbj|BAC98955.1| gentisate 1,2-dioxygenase [Xanthobacter polyaromaticivorans] Length = 350 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 213/344 (61%), Positives = 262/344 (76%) Query: 2 EAVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKL 61 +A T ER+ FYKKID Q+ +ALW V+N++ITPEP+S C PH+W+F + +DY+ EAG L Sbjct: 5 KAPMDTAERQNFYKKIDDQSYTALWTVLNNIITPEPRSNCLPHVWRFHEAKDYLLEAGGL 64 Query: 62 ITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGA 121 ITAKEAERRVLVLENPGLRGQS+ITTSL+AG+Q+V+P ++APAHRH+QSALR VL+G GA Sbjct: 65 ITAKEAERRVLVLENPGLRGQSRITTSLYAGLQIVMPDEVAPAHRHSQSALRLVLDGNGA 124 Query: 122 FTAVDGERTAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKED 181 T+VDGERT MS GDF+ITP TWHDH N T+EPM WLDGLDI +V FD SFAE D Sbjct: 125 HTSVDGERTIMSFGDFIITPPWTWHDHGNSTDEPMIWLDGLDIAVVSLFDASFAEEYGAD 184 Query: 182 QQSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWDACHGL 241 +Q I G S ARYG NL+P+D SPIFNYPYE +REALE K EWD CHGL Sbjct: 185 EQPIVHNIGDSSARYGANLMPVDFVHKGLASPIFNYPYERSREALEALKKSQEWDRCHGL 244 Query: 242 KLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWG 301 K++F NP G +AMPTI T +QLLPKGF A YR+TDA VF +EG G++++G + WG Sbjct: 245 KMRFVNPVDGGWAMPTIATGLQLLPKGFSAAPYRSTDAMVFVVLEGSGKSQIGGKEIIWG 304 Query: 302 PRDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDRGN 345 +D+FV PSWHWVTH ++V F++SDR Q+KL L+REDRGN Sbjct: 305 KKDVFVAPSWHWVTHMPLEETVFFTYSDRVAQEKLGLWREDRGN 348