BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0129.0026.p (302 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAJ23986.1| haloalkane dehalogenase DbeA [Bradyrhizobium elk... 575 e-162 pdb|3A2L|A Chain A, Crystal Structure Of Dbja (Mutant Dbja Delta... 424 e-117 pdb|3A2M|A Chain A, Crystal Structure Of Dbja (Wild Type Type I)... 419 e-115 pdb|3AFI|E Chain E, Crystal Structure Of Dbja (His-Dbja) >gi|319... 419 e-115 ref|NP_767727.1| haloalkane dehalogenase [Bradyrhizobium japonic... 419 e-115 >dbj|BAJ23986.1| haloalkane dehalogenase DbeA [Bradyrhizobium elkanii USDA 94] Length = 310 Score = 575 bits (1481), Expect = e-162, Method: Compositional matrix adjust. Identities = 289/302 (95%), Positives = 293/302 (97%) Query: 1 MTISADISLHHRAVLGSTMAYCETGRGDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 MTISADISLHHRAVLGSTMAY ETGR DAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA Sbjct: 1 MTISADISLHHRAVLGSTMAYRETGRSDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 Query: 61 PDLIGYGQSGKPDIGYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 PDLIGYGQSGKPDI YRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV Sbjct: 61 PDLIGYGQSGKPDISYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 Query: 121 RGLAFMEFIRPMRDWSDFHQHDAARETFRKFRTPGVGEAMVLDNNAFVERVLPGSILRTL 180 RGLAFMEFIRPMRDWSDFHQHDAARETFRKFRTPGVGEAM+LDNNAFVERVLPGSILRTL Sbjct: 121 RGLAFMEFIRPMRDWSDFHQHDAARETFRKFRTPGVGEAMILDNNAFVERVLPGSILRTL 180 Query: 181 SEEEMAAYRAPFATRESRKPTLMLPRELPIAGEPADVAQALTVAHAALAASTYPKLLFVG 240 SEEEMAAYRAPFATRESR PTLMLPRELPIAGEPADV QALT AHAALAASTYPKLLFVG Sbjct: 181 SEEEMAAYRAPFATRESRMPTLMLPRELPIAGEPADVTQALTAAHAALAASTYPKLLFVG 240 Query: 241 TPGALVSPTFAAEFAKTLRHCAVIQLGAGAHYLQEDHPETIGRSVAGWIAGVEAASAQRH 300 +PGALVSP FAAEFAKTL+HCAVIQLGAG HYLQEDHPE IGRSVAGWIAG+EAASAQRH Sbjct: 241 SPGALVSPAFAAEFAKTLKHCAVIQLGAGGHYLQEDHPEAIGRSVAGWIAGIEAASAQRH 300 Query: 301 AA 302 AA Sbjct: 301 AA 302 >pdb|3A2L|A Chain A, Crystal Structure Of Dbja (Mutant Dbja Delta) pdb|3A2L|B Chain B, Crystal Structure Of Dbja (Mutant Dbja Delta) Length = 309 Score = 424 bits (1090), Expect = e-117, Method: Compositional matrix adjust. Identities = 217/300 (72%), Positives = 241/300 (80%), Gaps = 2/300 (0%) Query: 1 MTISADISLHHRAVLGSTMAYCETGRGDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 M+ +I + VLGS+MAY ETG DAP VLFLHGNPTSS+IWRNI+PLV+PV HCIA Sbjct: 1 MSKPIEIEIRRAPVLGSSMAYRETGAQDAPVVLFLHGNPTSSHIWRNILPLVSPVAHCIA 60 Query: 61 PDLIGYGQSGKPDIGYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 PDLIG+GQSGKPDI YRFFD YLDA I++ G+ SAYLVAQDWGTALAFHLAARRP V Sbjct: 61 PDLIGFGQSGKPDIAYRFFDHVRYLDAFIEQRGVTSAYLVAQDWGTALAFHLAARRPDFV 120 Query: 121 RGLAFMEFIRPMRDWSDFHQH--DAARETFRKFRTPGVGEAMVLDNNAFVERVLPGSILR 178 RGLAFMEFIRPM W DFHQ +AAR FRKFRTPG GEAM+L+ NAFVERVLPG I+R Sbjct: 121 RGLAFMEFIRPMPTWQDFHQDHAEAARAVFRKFRTPGEGEAMILEANAFVERVLPGGIVR 180 Query: 179 TLSEEEMAAYRAPFATRESRKPTLMLPRELPIAGEPADVAQALTVAHAALAASTYPKLLF 238 L +EEMA YR PF T ESR+P L PRELPIAGEPADV +AL AHAALAAS+YPKLLF Sbjct: 181 KLGDEEMAPYRTPFPTPESRRPVLAFPRELPIAGEPADVYEALQSAHAALAASSYPKLLF 240 Query: 239 VGTPGALVSPTFAAEFAKTLRHCAVIQLGAGAHYLQEDHPETIGRSVAGWIAGVEAASAQ 298 G PGALVSP FA FA +L CA+I+LGAG HYLQEDH + IGRSVAGWIAG+EA Q Sbjct: 241 TGEPGALVSPEFAERFAASLTRCALIRLGAGLHYLQEDHADAIGRSVAGWIAGIEAVRPQ 300 >pdb|3A2M|A Chain A, Crystal Structure Of Dbja (Wild Type Type I) pdb|3A2M|B Chain B, Crystal Structure Of Dbja (Wild Type Type I) pdb|3A2N|A Chain A, Crystal Structure Of Dbja (Wild Type Type Ii P21) pdb|3A2N|B Chain B, Crystal Structure Of Dbja (Wild Type Type Ii P21) pdb|3A2N|E Chain E, Crystal Structure Of Dbja (Wild Type Type Ii P21) pdb|3A2N|F Chain F, Crystal Structure Of Dbja (Wild Type Type Ii P21) Length = 312 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 216/307 (70%), Positives = 240/307 (78%), Gaps = 9/307 (2%) Query: 1 MTISADISLHHRAVLGSTMAYCETGRGDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 M+ +I + VLGS+MAY ETG DAP VLFLHGNPTSS+IWRNI+PLV+PV HCIA Sbjct: 1 MSKPIEIEIRRAPVLGSSMAYRETGAQDAPVVLFLHGNPTSSHIWRNILPLVSPVAHCIA 60 Query: 61 PDLIGYGQSGKPDIGYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 PDLIG+GQSGKPDI YRFFD YLDA I++ G+ SAYLVAQDWGTALAFHLAARRP V Sbjct: 61 PDLIGFGQSGKPDIAYRFFDHVRYLDAFIEQRGVTSAYLVAQDWGTALAFHLAARRPDFV 120 Query: 121 RGLAFMEFIRPMRDWSDFHQHD---------AARETFRKFRTPGVGEAMVLDNNAFVERV 171 RGLAFMEFIRPM W DFH + AAR FRKFRTPG GEAM+L+ NAFVERV Sbjct: 121 RGLAFMEFIRPMPTWQDFHHTEVAEEQDHAEAARAVFRKFRTPGEGEAMILEANAFVERV 180 Query: 172 LPGSILRTLSEEEMAAYRAPFATRESRKPTLMLPRELPIAGEPADVAQALTVAHAALAAS 231 LPG I+R L +EEMA YR PF T ESR+P L PRELPIAGEPADV +AL AHAALAAS Sbjct: 181 LPGGIVRKLGDEEMAPYRTPFPTPESRRPVLAFPRELPIAGEPADVYEALQSAHAALAAS 240 Query: 232 TYPKLLFVGTPGALVSPTFAAEFAKTLRHCAVIQLGAGAHYLQEDHPETIGRSVAGWIAG 291 +YPKLLF G PGALVSP FA FA +L CA+I+LGAG HYLQEDH + IGRSVAGWIAG Sbjct: 241 SYPKLLFTGEPGALVSPEFAERFAASLTRCALIRLGAGLHYLQEDHADAIGRSVAGWIAG 300 Query: 292 VEAASAQ 298 +EA Q Sbjct: 301 IEAVRPQ 307 >pdb|3AFI|E Chain E, Crystal Structure Of Dbja (His-Dbja) pdb|3AFI|A Chain A, Crystal Structure Of Dbja (His-Dbja) pdb|3AFI|B Chain B, Crystal Structure Of Dbja (His-Dbja) pdb|3AFI|F Chain F, Crystal Structure Of Dbja (His-Dbja) Length = 316 Score = 419 bits (1076), Expect = e-115, Method: Compositional matrix adjust. Identities = 216/307 (70%), Positives = 240/307 (78%), Gaps = 9/307 (2%) Query: 1 MTISADISLHHRAVLGSTMAYCETGRGDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 M+ +I + VLGS+MAY ETG DAP VLFLHGNPTSS+IWRNI+PLV+PV HCIA Sbjct: 1 MSKPIEIEIRRAPVLGSSMAYRETGAQDAPVVLFLHGNPTSSHIWRNILPLVSPVAHCIA 60 Query: 61 PDLIGYGQSGKPDIGYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 PDLIG+GQSGKPDI YRFFD YLDA I++ G+ SAYLVAQDWGTALAFHLAARRP V Sbjct: 61 PDLIGFGQSGKPDIAYRFFDHVRYLDAFIEQRGVTSAYLVAQDWGTALAFHLAARRPDFV 120 Query: 121 RGLAFMEFIRPMRDWSDFHQHD---------AARETFRKFRTPGVGEAMVLDNNAFVERV 171 RGLAFMEFIRPM W DFH + AAR FRKFRTPG GEAM+L+ NAFVERV Sbjct: 121 RGLAFMEFIRPMPTWQDFHHTEVAEEQDHAEAARAVFRKFRTPGEGEAMILEANAFVERV 180 Query: 172 LPGSILRTLSEEEMAAYRAPFATRESRKPTLMLPRELPIAGEPADVAQALTVAHAALAAS 231 LPG I+R L +EEMA YR PF T ESR+P L PRELPIAGEPADV +AL AHAALAAS Sbjct: 181 LPGGIVRKLGDEEMAPYRTPFPTPESRRPVLAFPRELPIAGEPADVYEALQSAHAALAAS 240 Query: 232 TYPKLLFVGTPGALVSPTFAAEFAKTLRHCAVIQLGAGAHYLQEDHPETIGRSVAGWIAG 291 +YPKLLF G PGALVSP FA FA +L CA+I+LGAG HYLQEDH + IGRSVAGWIAG Sbjct: 241 SYPKLLFTGEPGALVSPEFAERFAASLTRCALIRLGAGLHYLQEDHADAIGRSVAGWIAG 300 Query: 292 VEAASAQ 298 +EA Q Sbjct: 301 IEAVRPQ 307 >ref|NP_767727.1| haloalkane dehalogenase [Bradyrhizobium japonicum USDA 110] sp|P59337.1|DHAA_BRAJA RecName: Full=Haloalkane dehalogenase dbj|BAC46352.1| haloalkane dehalogenase [Bradyrhizobium japonicum USDA 110] Length = 310 Score = 419 bits (1076), Expect = e-115, Method: Compositional matrix adjust. Identities = 216/307 (70%), Positives = 240/307 (78%), Gaps = 9/307 (2%) Query: 1 MTISADISLHHRAVLGSTMAYCETGRGDAPHVLFLHGNPTSSYIWRNIMPLVAPVGHCIA 60 M+ +I + VLGS+MAY ETG DAP VLFLHGNPTSS+IWRNI+PLV+PV HCIA Sbjct: 1 MSKPIEIEIRRAPVLGSSMAYRETGAQDAPVVLFLHGNPTSSHIWRNILPLVSPVAHCIA 60 Query: 61 PDLIGYGQSGKPDIGYRFFDQADYLDALIDELGIASAYLVAQDWGTALAFHLAARRPQLV 120 PDLIG+GQSGKPDI YRFFD YLDA I++ G+ SAYLVAQDWGTALAFHLAARRP V Sbjct: 61 PDLIGFGQSGKPDIAYRFFDHVRYLDAFIEQRGVTSAYLVAQDWGTALAFHLAARRPDFV 120 Query: 121 RGLAFMEFIRPMRDWSDFHQHD---------AARETFRKFRTPGVGEAMVLDNNAFVERV 171 RGLAFMEFIRPM W DFH + AAR FRKFRTPG GEAM+L+ NAFVERV Sbjct: 121 RGLAFMEFIRPMPTWQDFHHTEVAEEQDHAEAARAVFRKFRTPGEGEAMILEANAFVERV 180 Query: 172 LPGSILRTLSEEEMAAYRAPFATRESRKPTLMLPRELPIAGEPADVAQALTVAHAALAAS 231 LPG I+R L +EEMA YR PF T ESR+P L PRELPIAGEPADV +AL AHAALAAS Sbjct: 181 LPGGIVRKLGDEEMAPYRTPFPTPESRRPVLAFPRELPIAGEPADVYEALQSAHAALAAS 240 Query: 232 TYPKLLFVGTPGALVSPTFAAEFAKTLRHCAVIQLGAGAHYLQEDHPETIGRSVAGWIAG 291 +YPKLLF G PGALVSP FA FA +L CA+I+LGAG HYLQEDH + IGRSVAGWIAG Sbjct: 241 SYPKLLFTGEPGALVSPEFAERFAASLTRCALIRLGAGLHYLQEDHADAIGRSVAGWIAG 300 Query: 292 VEAASAQ 298 +EA Q Sbjct: 301 IEAVRPQ 307