BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0127.0034.p (286 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002822633.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarbo... 327 2e-87 ref|YP_840535.1| 5-carboxymethyl-2-hydroxymuconate delta-isomera... 322 4e-86 ref|ZP_07029814.1| Ureidoglycolate lyase [Acidobacterium sp. MP5... 322 5e-86 ref|YP_624742.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxyl... 316 3e-84 ref|ZP_04942763.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 310 1e-82 >ref|YP_002822633.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Sinorhizobium fredii NGR234] gb|AAQ87342.1| Fumarylacetoacetate hydrolase family protein [Sinorhizobium fredii NGR234] gb|ACP21880.1| putative 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Sinorhizobium fredii NGR234] Length = 291 Score = 327 bits (837), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 166/285 (58%), Positives = 207/285 (72%), Gaps = 2/285 (0%) Query: 1 MKFLSYIKDSAHGIALSIDEQVLGLSSDDPNYPGSLDDLIGGPPTALADAAQTLIGRGEP 60 M+FL+Y + + G+A+ + + G + Y GS+ I AL A L R Sbjct: 1 MRFLAYTQGNQTGVAVLSNGEWRGAADAGTTYAGSIQAWIEQGGEALWLAGDRL-SREPI 59 Query: 61 LDLADLTLLPPC-TPGKIICVGLNYADHAAEANLQVPEHPVLFSRFASSVIGAGAPIWLP 119 +DLA++ LLPP PGKI+CVGLNYADH E+ + P+HP LF RF SS+I AG PI P Sbjct: 60 VDLANVDLLPPILRPGKIVCVGLNYADHTEESGYKQPDHPTLFPRFTSSLIAAGEPIVRP 119 Query: 120 RVSTALDFEGELAAIIGKPGRYIGEENALDHVVGYSVFNDATIRDYQLRNPQWMIGKNFD 179 VS LDFEGEL A+IG+ GR+I + +ALDHV GYS+FNDA+IR++Q R PQW +GKNFD Sbjct: 120 FVSETLDFEGELVAVIGRRGRHISKHDALDHVAGYSIFNDASIREFQHRTPQWTLGKNFD 179 Query: 180 GTGPFGPTLVTSDELPPGASGLAIETRLNGVTVQKSSTDQLIFNVGRLIASISEAITLEP 239 GTG FGP VT+DELP GA+GL IETRLNG VQ S+TDQLIF+V L+++ISEAITLEP Sbjct: 180 GTGSFGPWFVTADELPRGAAGLRIETRLNGEVVQASNTDQLIFDVATLVSTISEAITLEP 239 Query: 240 RDVIVTGTPSGVGHVRKPQLHMRSGDVVEVEIERIGLLRNPVTPE 284 D+IVTGTPSG+GH R P+L+MR+GDVVEVEI+ IG LRNPV E Sbjct: 240 GDLIVTGTPSGIGHARNPRLYMRAGDVVEVEIQSIGTLRNPVVDE 284 >ref|YP_840535.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia HI2424] gb|ABK13642.1| 5-carboxymethyl-2-hydroxymuconate delta-isomerase [Burkholderia cenocepacia HI2424] Length = 290 Score = 322 bits (826), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 161/289 (55%), Positives = 203/289 (70%), Gaps = 7/289 (2%) Query: 1 MKFLSYIKDSAHGIALSIDEQVLGLSSDDPNYPGSLDDLIGGPPTALADAAQ--TLIGRG 58 M+F Y KD GIA+ + GL+++D YPG LD L+ AD A+ L+ RG Sbjct: 1 MRFAVYKKDGHRGIAVEHGGRWFGLTAEDTAYPGDLDALLAHG----ADLAEVGALLARG 56 Query: 59 EPLDLADLTLLPPC-TPGKIICVGLNYADHAAEANLQVPEHPVLFSRFASSVIGAGAPIW 117 P+DL + LLPP GKI+CVGLNY DH+ E +P +F+RFASS+IG G PI Sbjct: 57 APVDLEAVRLLPPFGRSGKIVCVGLNYLDHSTETGFAKQAYPTVFARFASSLIGHGDPIA 116 Query: 118 LPRVSTALDFEGELAAIIGKPGRYIGEENALDHVVGYSVFNDATIRDYQLRNPQWMIGKN 177 LP S+ LD+EGEL A+IGK GR I E ALDHV GYS+FNDAT+RDYQ R PQW +GKN Sbjct: 117 LPAQSSQLDYEGELVAVIGKGGRSIARERALDHVAGYSIFNDATLRDYQFRTPQWTMGKN 176 Query: 178 FDGTGPFGPTLVTSDELPPGASGLAIETRLNGVTVQKSSTDQLIFNVGRLIASISEAITL 237 FDGTG FGP VT+DELP G GL I+TRLNG VQ++ST +IF+V L+ +S+ +TL Sbjct: 177 FDGTGAFGPWFVTADELPRGCKGLKIQTRLNGAVVQEASTSDMIFDVETLVVLLSDVMTL 236 Query: 238 EPRDVIVTGTPSGVGHVRKPQLHMRSGDVVEVEIERIGLLRNPVTPEAI 286 + DVIVTGTP+G+G R+PQL M++GDV EVEIER+G+LRNPV P A+ Sbjct: 237 QAGDVIVTGTPAGIGMAREPQLWMKAGDVCEVEIERLGVLRNPVMPAAM 285 >ref|ZP_07029814.1| Ureidoglycolate lyase [Acidobacterium sp. MP5ACTX8] gb|EFI57301.1| Ureidoglycolate lyase [Acidobacterium sp. MP5ACTX8] Length = 285 Score = 322 bits (824), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 160/286 (55%), Positives = 209/286 (73%), Gaps = 3/286 (1%) Query: 1 MKFLSYIKDSAHGIALSI-DEQVLGLSSDDPNYPGSLDDLIGGPPTALADAAQTLIGRGE 59 M+F + + G+A++ + GL S D YPGSLD L+ +AL DAA ++ +G Sbjct: 1 MRFAVFSSEGREGLAVAAPGGEFHGLFSGDAGYPGSLDSLVRKGRSAL-DAAAVVLQKGF 59 Query: 60 PLDLADLTLLPPCT-PGKIICVGLNYADHAAEANLQVPEHPVLFSRFASSVIGAGAPIWL 118 +DL ++TL PP + PGKIICVGLNY DH+ E+ VP++P +F+RF SS++GAGAPI Sbjct: 60 AVDLNEVTLHPPLSAPGKIICVGLNYVDHSIESGFVVPDYPTIFTRFTSSLLGAGAPIIR 119 Query: 119 PRVSTALDFEGELAAIIGKPGRYIGEENALDHVVGYSVFNDATIRDYQLRNPQWMIGKNF 178 P VS LD+EGE+ A+IG GR+I E++ALDHV+GYS+FNDA++RDYQ ++PQW IGKNF Sbjct: 120 PTVSIQLDYEGEMVAVIGVAGRHIAEQDALDHVIGYSIFNDASVRDYQKKSPQWTIGKNF 179 Query: 179 DGTGPFGPTLVTSDELPPGASGLAIETRLNGVTVQKSSTDQLIFNVGRLIASISEAITLE 238 D TG FGP LVT+DE+ PG GL I+TRLNG VQ +STD ++F+V RLI+ +SEAITL Sbjct: 180 DNTGAFGPYLVTADEVSPGGKGLHIQTRLNGTVVQDASTDDMVFSVARLISILSEAITLS 239 Query: 239 PRDVIVTGTPSGVGHVRKPQLHMRSGDVVEVEIERIGLLRNPVTPE 284 P D+ VTGTP+GVG RKP L M+ GD EVEIE IG+LRN V E Sbjct: 240 PGDIFVTGTPAGVGMARKPPLFMKHGDNCEVEIEGIGILRNTVQDE 285 >ref|YP_624742.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Burkholderia cenocepacia AU 1054] ref|YP_836908.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Burkholderia cenocepacia HI2424] gb|ABF79769.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Burkholderia cenocepacia AU 1054] gb|ABK10015.1| 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase [Burkholderia cenocepacia HI2424] Length = 290 Score = 316 bits (809), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 158/284 (55%), Positives = 199/284 (70%), Gaps = 7/284 (2%) Query: 1 MKFLSYIKDSAHGIALSIDEQVLGLSSDDPNYPGSLDDLIGGPPTALADAAQ--TLIGRG 58 M+F Y D GIA+ + GL+++D YPG LD L+ AD A+ L+ RG Sbjct: 1 MRFAVYKTDGHRGIAVEHGGRWFGLTAEDTAYPGDLDALLAHG----ADLAEVGALLARG 56 Query: 59 EPLDLADLTLLPPC-TPGKIICVGLNYADHAAEANLQVPEHPVLFSRFASSVIGAGAPIW 117 P+DL + LLPP GKI+CVGLNY DH+ E +P +F+RFASS+IG G PI Sbjct: 57 APVDLEAVRLLPPFGRSGKIVCVGLNYLDHSTETGFAKQAYPTVFARFASSLIGHGDPIA 116 Query: 118 LPRVSTALDFEGELAAIIGKPGRYIGEENALDHVVGYSVFNDATIRDYQLRNPQWMIGKN 177 LP S+ LD+EGEL A+IGK GR I E ALDHV GYS+FNDAT+RDYQ R PQW +GKN Sbjct: 117 LPAQSSQLDYEGELVAVIGKGGRSIARERALDHVAGYSIFNDATLRDYQFRTPQWTMGKN 176 Query: 178 FDGTGPFGPTLVTSDELPPGASGLAIETRLNGVTVQKSSTDQLIFNVGRLIASISEAITL 237 FDGTG FGP VT+DELP G GL I+TRLNG VQ++ST +IF+V L+ +S+ +TL Sbjct: 177 FDGTGAFGPWFVTADELPRGCKGLKIQTRLNGAVVQEASTSDMIFDVETLVVLLSDVMTL 236 Query: 238 EPRDVIVTGTPSGVGHVRKPQLHMRSGDVVEVEIERIGLLRNPV 281 + DVIVTGTP+G+G R+PQL M++GDV EVEIER+G+LRNPV Sbjct: 237 QAGDVIVTGTPAGIGMAREPQLWMKAGDVCEVEIERLGVLRNPV 280 >ref|ZP_04942763.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Burkholderia cenocepacia PC184] gb|EAY65934.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Burkholderia cenocepacia PC184] Length = 306 Score = 310 bits (795), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 155/287 (54%), Positives = 199/287 (69%), Gaps = 3/287 (1%) Query: 1 MKFLSYIKDSAHGIALSIDEQVLGLSSDDPNYPGSLDDLIGGPPTALADAAQTLIGRGEP 60 M+F Y K+ GIA+ Q GL+++D YPG LD L+ LA+ A L RG P Sbjct: 17 MRFAIYKKNGHRGIAVEHSGQWFGLTAEDTAYPGDLDSLLA-QGADLAEVAARL-ARGTP 74 Query: 61 LDLADLTLLPPC-TPGKIICVGLNYADHAAEANLQVPEHPVLFSRFASSVIGAGAPIWLP 119 +DL + +LPP P KI+CVGLNY DH+ E +P +F+RFASS+IG PI LP Sbjct: 75 VDLDAVRVLPPFGRPNKILCVGLNYLDHSMETGFAKQTYPTVFARFASSLIGHDQPIALP 134 Query: 120 RVSTALDFEGELAAIIGKPGRYIGEENALDHVVGYSVFNDATIRDYQLRNPQWMIGKNFD 179 S+ LD+EGEL A+IGK GR I E ALDHV GYS+FND T+RDYQ R PQW +GKNFD Sbjct: 135 AQSSQLDYEGELVAVIGKAGRGITRERALDHVAGYSLFNDVTLRDYQFRTPQWTMGKNFD 194 Query: 180 GTGPFGPTLVTSDELPPGASGLAIETRLNGVTVQKSSTDQLIFNVGRLIASISEAITLEP 239 GTG FGP VT+DELP G GL I+TRLNG VQ++ST +IF++ L+ +SE +TL+ Sbjct: 195 GTGGFGPWFVTADELPRGCKGLRIQTRLNGAVVQEASTSDMIFDIETLVVMLSEVMTLQA 254 Query: 240 RDVIVTGTPSGVGHVRKPQLHMRSGDVVEVEIERIGLLRNPVTPEAI 286 D+IVTGTP+G+G R+PQL M++GDV EVEIE++G+LRN V P A+ Sbjct: 255 GDLIVTGTPAGIGMAREPQLWMKAGDVCEVEIEQLGVLRNAVMPAAM 301