BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0120.0085.p (226 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002490207.1| Enoyl-CoA hydratase/isomerase [Methylobacter... 150 1e-34 ref|ZP_02886054.1| Enoyl-CoA hydratase/isomerase [Burkholderia g... 149 3e-34 ref|YP_002498507.1| enoyl-CoA hydratase/isomerase [Methylobacter... 140 2e-31 ref|YP_001768288.1| enoyl-CoA hydratase/isomerase [Methylobacter... 120 1e-25 ref|ZP_05084827.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibri... 107 1e-21 >ref|YP_002490207.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] gb|ACL63040.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] Length = 247 Score = 150 bits (379), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 84/187 (44%), Positives = 118/187 (63%), Gaps = 2/187 (1%) Query: 7 DDVGAVVLAGTAPGFCAGSDLKFIGRLELEAMGRFEQECGDLGRLIGYLAKPVVAAVEKF 66 + V A+VL G P FCAGSDLK +G L + AM E E + R IG+L KPVVAAVE + Sbjct: 43 ESVRAIVLTGAPPAFCAGSDLKELGGLSIPAMCDHEAETATVARSIGFLTKPVVAAVEGY 102 Query: 67 A--GGLTLATSCDVVVAGRSSVFSLPEVPHGWLTPWGIRSLVARVGLVKARLLCFCLEAL 124 A GG LA SCDVVV+ ++ + L EV +GWL WG+++L+ARVG V+ARLL + +E++ Sbjct: 103 ALGGGFILAVSCDVVVSAENARWHLAEVTNGWLPVWGLQALLARVGPVRARLLTWGVESI 162 Query: 125 KSEDARTIGIVDYCCDDGEALTRAIAIAEKLAQLPRPAVRATXAFSRTILWKTLKRWISK 184 +A +G+ DY + G AL RA+A+A+ LA+LP AV +T F + +R + Sbjct: 163 DGREALRLGVADYTAEAGGALDRALAVAQGLAKLPPEAVTSTKRFFEPFVMADAERLDNV 222 Query: 185 QIVYFSR 191 F+R Sbjct: 223 ASRMFAR 229 >ref|ZP_02886054.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] gb|EDT08442.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] Length = 246 Score = 149 bits (377), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 78/168 (46%), Positives = 112/168 (66%), Gaps = 2/168 (1%) Query: 4 DRNDDVGAVVLAGTAPGFCAGSDLKFIGRLELEAMGRFEQECGDLGRLIGYLAKPVVAAV 63 + + V A+VL+G P FCAGSDLK +G L ++ M E E + R I L+KPV+AAV Sbjct: 39 ESDASVAAIVLSGAPPAFCAGSDLKELGGLSIDHMCEHELETARIARGIAGLSKPVIAAV 98 Query: 64 EKFA--GGLTLATSCDVVVAGRSSVFSLPEVPHGWLTPWGIRSLVARVGLVKARLLCFCL 121 E FA GG LA SCD+VV+ ++ + LPEVP+GWL PWG+++L+ARVG V+AR+L + Sbjct: 99 EGFALGGGFILAVSCDLVVSAVNARWHLPEVPNGWLPPWGLQALIARVGAVRARMLVWGD 158 Query: 122 EALKSEDARTIGIVDYCCDDGEALTRAIAIAEKLAQLPRPAVRATXAF 169 E + +A +G+ D+ + G RAIA+A++LA+LPR AV + F Sbjct: 159 EPIDGVEAHRLGVADHLAEAGHVDARAIALAQRLARLPRGAVASAKRF 206 >ref|YP_002498507.1| enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] gb|ACL58204.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] Length = 250 Score = 140 bits (352), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 82/170 (48%), Positives = 102/170 (60%), Gaps = 3/170 (1%) Query: 3 LDRNDDVGAVVLAGTAPGFCAGSDLKFIGRLELEAMGRFEQECGDLGRLIGYLAKPVVAA 62 LDR+ D A+VL G APGFCAGSDLK + +L M E G L R I +L PV+AA Sbjct: 41 LDRDADTHAIVLTGAAPGFCAGSDLKELADTDLTGMCAHEAHTGALARSIAFLDTPVIAA 100 Query: 63 VEKFA--GGLTLATSCDVVVAGRSSVFSLPEVPHGWLTPWGIRSLVARVGLVKARLLCFC 120 V+ FA GG LA SCDVVV G + + LPEV GW+ PWG++++VARVG V AR L + Sbjct: 101 VDGFALGGGFVLAISCDVVVTGAEARWHLPEVTIGWIPPWGLQAVVARVGPVAARRLTWG 160 Query: 121 LEALKSEDARTIGIVDYCCDDGEA-LTRAIAIAEKLAQLPRPAVRATXAF 169 +A +GI D GE+ L A+ AE LA LP PAV +T F Sbjct: 161 AAPFDGREAHRLGIADTLVAPGESVLDAALREAEGLAALPPPAVASTKQF 210 >ref|YP_001768288.1| enoyl-CoA hydratase/isomerase [Methylobacterium sp. 4-46] gb|ACA15854.1| Enoyl-CoA hydratase/isomerase [Methylobacterium sp. 4-46] Length = 250 Score = 120 bits (302), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 77/170 (45%), Positives = 98/170 (57%), Gaps = 3/170 (1%) Query: 3 LDRNDDVGAVVLAGTAPGFCAGSDLKFIGRLELEAMGRFEQECGDLGRLIGYLAKPVVAA 62 LDR D A+VL G APGFCAGSDLK + +L M E L R I +L PV+AA Sbjct: 41 LDREADTRAIVLTGAAPGFCAGSDLKELAETDLAGMCAHEARTAALARSIAFLGTPVIAA 100 Query: 63 VEKFA--GGLTLATSCDVVVAGRSSVFSLPEVPHGWLTPWGIRSLVARVGLVKARLLCFC 120 V+ FA GG LA SCDVVV + + LPEV GW+ PWG++++VARVG V AR L + Sbjct: 101 VDGFALGGGFVLAISCDVVVTASEARWHLPEVSIGWIPPWGLQAVVARVGPVAARRLTWG 160 Query: 121 LEALKSEDARTIGIVDYCCDDGEAL-TRAIAIAEKLAQLPRPAVRATXAF 169 +A +G+ D G ++ A+ AE LA LP PAV +T F Sbjct: 161 AAPFDGREAHRLGLADALVAPGASVLEAALREAEALAALPPPAVASTKHF 210 >ref|ZP_05084827.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibrio sp. JE062] gb|EEA94769.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibrio sp. JE062] Length = 271 Score = 107 bits (267), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 63/162 (38%), Positives = 91/162 (56%), Gaps = 2/162 (1%) Query: 7 DDVGAVVLAGTAPGFCAGSDLKFIGRLELEAMGRFEQECGDLGRLIGYLAKPVVAAVEKF 66 D + A V+ G P FCAGSDL +G L+L+ + E+ R I KP+VAAV+ + Sbjct: 69 DTINAAVIFGADPSFCAGSDLTELGALDLDGICDVEKRKAAFLRRISLCPKPIVAAVQGY 128 Query: 67 A--GGLTLATSCDVVVAGRSSVFSLPEVPHGWLTPWGIRSLVARVGLVKARLLCFCLEAL 124 A GG LA +CDVV+A + F EVP+GW+TPWG +L AR+ A+ + + + Sbjct: 129 AVGGGAFLAAACDVVIAAENVKFKGMEVPNGWITPWGFHALNARMAPRHAQRAAWGYKFM 188 Query: 125 KSEDARTIGIVDYCCDDGEALTRAIAIAEKLAQLPRPAVRAT 166 + +A G+VD + A IAE+LA LP +V+AT Sbjct: 189 TATEALRTGLVDEVVALDDLFETARTIAEQLAALPPVSVQAT 230