BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0120.0077.p (247 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_002490207.1| Enoyl-CoA hydratase/isomerase [Methylobacter... 322 4e-86 ref|ZP_02886054.1| Enoyl-CoA hydratase/isomerase [Burkholderia g... 281 9e-74 ref|YP_002498507.1| enoyl-CoA hydratase/isomerase [Methylobacter... 255 4e-66 ref|YP_001768288.1| enoyl-CoA hydratase/isomerase [Methylobacter... 250 1e-64 ref|ZP_05084827.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibri... 192 3e-47 >ref|YP_002490207.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] gb|ACL63040.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] Length = 247 Score = 322 bits (824), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 163/247 (65%), Positives = 196/247 (79%) Query: 1 MIKLDNQGPIAIVTINRAERRNALGSQLMRDLNAALADSERNDAISAIVLTGAPPAFCAG 60 M++ + +G +AIVT++RA RRNALG++ MR L+AAL ++ ++++ AIVLTGAPPAFCAG Sbjct: 1 MVRSETRGSVAIVTLDRAARRNALGTESMRALSAALRAADADESVRAIVLTGAPPAFCAG 60 Query: 61 SDLKELAGLSIADMCKHEAETATFARAIGYRAKPVIAAVEGYALGGGMILAASCDIVVAA 120 SDLKEL GLSI MC HEAETAT AR+IG+ KPV+AAVEGYALGGG ILA SCD+VV+A Sbjct: 61 SDLKELGGLSIPAMCDHEAETATVARSIGFLTKPVVAAVEGYALGGGFILAVSCDVVVSA 120 Query: 121 SNARWHLPEAPNGWLPPWGLTALLTRVGPVRARRLTWGAASIDTIEAARIGLVDDVAEPG 180 NARWHL E NGWLP WGL ALL RVGPVRAR LTWG SID EA R+G+ D AE G Sbjct: 121 ENARWHLAEVTNGWLPVWGLQALLARVGPVRARLLTWGVESIDGREALRLGVADYTAEAG 180 Query: 181 AALSLATEIAVGLSKVPPEAVASTKRFFDRFIMADAERLDEVSSREFASNCETPSSQKTF 240 AL A +A GL+K+PPEAV STKRFF+ F+MADAERLD V+SR FA +CE+ S+Q T Sbjct: 181 GALDRALAVAQGLAKLPPEAVTSTKRFFEPFVMADAERLDNVASRMFARDCESASAQATL 240 Query: 241 ERFTVKS 247 RFTV+S Sbjct: 241 ARFTVRS 247 >ref|ZP_02886054.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] gb|EDT08442.1| Enoyl-CoA hydratase/isomerase [Burkholderia graminis C4D1M] Length = 246 Score = 281 bits (718), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 137/246 (55%), Positives = 178/246 (72%) Query: 2 IKLDNQGPIAIVTINRAERRNALGSQLMRDLNAALADSERNDAISAIVLTGAPPAFCAGS 61 +++++ G + + ++R +RRNAL LM L L +E + +++AIVL+GAPPAFCAGS Sbjct: 1 MQIEDIGGVRQLVLDRPQRRNALSQALMEQLEQELRRAESDASVAAIVLSGAPPAFCAGS 60 Query: 62 DLKELAGLSIADMCKHEAETATFARAIGYRAKPVIAAVEGYALGGGMILAASCDIVVAAS 121 DLKEL GLSI MC+HE ETA AR I +KPVIAAVEG+ALGGG ILA SCD+VV+A Sbjct: 61 DLKELGGLSIDHMCEHELETARIARGIAGLSKPVIAAVEGFALGGGFILAVSCDLVVSAV 120 Query: 122 NARWHLPEAPNGWLPPWGLTALLTRVGPVRARRLTWGAASIDTIEAARIGLVDDVAEPGA 181 NARWHLPE PNGWLPPWGL AL+ RVG VRAR L WG ID +EA R+G+ D +AE G Sbjct: 121 NARWHLPEVPNGWLPPWGLQALIARVGAVRARMLVWGDEPIDGVEAHRLGVADHLAEAGH 180 Query: 182 ALSLATEIAVGLSKVPPEAVASTKRFFDRFIMADAERLDEVSSREFASNCETPSSQKTFE 241 + A +A L+++P AVAS KRFF+ FI D ERLD ++R FA +C++ ++Q+ F Sbjct: 181 VDARAIALAQRLARLPRGAVASAKRFFEPFIALDGERLDREATRIFADDCKSDAAQQIFA 240 Query: 242 RFTVKS 247 +F VKS Sbjct: 241 KFAVKS 246 >ref|YP_002498507.1| enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] gb|ACL58204.1| Enoyl-CoA hydratase/isomerase [Methylobacterium nodulans ORS 2060] Length = 250 Score = 255 bits (652), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 129/247 (52%), Positives = 166/247 (67%), Gaps = 1/247 (0%) Query: 2 IKLDNQGPIAIVTINRAERRNALGSQLMRDLNAALADSERNDAISAIVLTGAPPAFCAGS 61 I ++ +G +A++TI+R RRNA+G QL+ DL A +R+ AIVLTGA P FCAGS Sbjct: 4 IVVERKGRVAVLTIDRPRRRNAIGVQLVEDLTREFARLDRDADTHAIVLTGAAPGFCAGS 63 Query: 62 DLKELAGLSIADMCKHEAETATFARAIGYRAKPVIAAVEGYALGGGMILAASCDIVVAAS 121 DLKELA + MC HEA T AR+I + PVIAAV+G+ALGGG +LA SCD+VV + Sbjct: 64 DLKELADTDLTGMCAHEAHTGALARSIAFLDTPVIAAVDGFALGGGFVLAISCDVVVTGA 123 Query: 122 NARWHLPEAPNGWLPPWGLTALLTRVGPVRARRLTWGAASIDTIEAARIGLVDDVAEPG- 180 ARWHLPE GW+PPWGL A++ RVGPV ARRLTWGAA D EA R+G+ D + PG Sbjct: 124 EARWHLPEVTIGWIPPWGLQAVVARVGPVAARRLTWGAAPFDGREAHRLGIADTLVAPGE 183 Query: 181 AALSLATEIAVGLSKVPPEAVASTKRFFDRFIMADAERLDEVSSREFASNCETPSSQKTF 240 + L A A GL+ +PP AVASTK+F+ + +AE D ++R FA +C P +Q T Sbjct: 184 SVLDAALREAEGLAALPPPAVASTKQFYAPLVAGNAEAADAWANRLFAGDCGHPVAQATL 243 Query: 241 ERFTVKS 247 RF VK+ Sbjct: 244 RRFGVKA 250 >ref|YP_001768288.1| enoyl-CoA hydratase/isomerase [Methylobacterium sp. 4-46] gb|ACA15854.1| Enoyl-CoA hydratase/isomerase [Methylobacterium sp. 4-46] Length = 250 Score = 250 bits (639), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 133/245 (54%), Positives = 165/245 (67%), Gaps = 1/245 (0%) Query: 4 LDNQGPIAIVTINRAERRNALGSQLMRDLNAALADSERNDAISAIVLTGAPPAFCAGSDL 63 ++ +G +AI+TI+R RRNA+G QL+ DL A +R AIVLTGA P FCAGSDL Sbjct: 6 VERKGSVAILTIDRPRRRNAIGVQLVEDLTREFARLDREADTRAIVLTGAAPGFCAGSDL 65 Query: 64 KELAGLSIADMCKHEAETATFARAIGYRAKPVIAAVEGYALGGGMILAASCDIVVAASNA 123 KELA +A MC HEA TA AR+I + PVIAAV+G+ALGGG +LA SCD+VV AS A Sbjct: 66 KELAETDLAGMCAHEARTAALARSIAFLGTPVIAAVDGFALGGGFVLAISCDVVVTASEA 125 Query: 124 RWHLPEAPNGWLPPWGLTALLTRVGPVRARRLTWGAASIDTIEAARIGLVDDVAEPGAAL 183 RWHLPE GW+PPWGL A++ RVGPV ARRLTWGAA D EA R+GL D + PGA++ Sbjct: 126 RWHLPEVSIGWIPPWGLQAVVARVGPVAARRLTWGAAPFDGREAHRLGLADALVAPGASV 185 Query: 184 -SLATEIAVGLSKVPPEAVASTKRFFDRFIMADAERLDEVSSREFASNCETPSSQKTFER 242 A A L+ +PP AVASTK F+ I AE D ++R FA++C P +Q T R Sbjct: 186 LEAALREAEALAALPPPAVASTKHFYAPAIAGTAEAADAWANRLFAADCGHPVAQGTLRR 245 Query: 243 FTVKS 247 F V + Sbjct: 246 FGVTA 250 >ref|ZP_05084827.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibrio sp. JE062] gb|EEA94769.1| 3-hydroxybutyryl-CoA dehydratase [Pseudovibrio sp. JE062] Length = 271 Score = 192 bits (489), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 97/242 (40%), Positives = 144/242 (59%) Query: 2 IKLDNQGPIAIVTINRAERRNALGSQLMRDLNAALADSERNDAISAIVLTGAPPAFCAGS 61 ++ + +A + +NR ++RNA +++ L+AA+ E D I+A V+ GA P+FCAGS Sbjct: 28 VEFSVENKVAYIQLNRPKQRNAQNKEMLDLLDAAMERVETCDTINAAVIFGADPSFCAGS 87 Query: 62 DLKELAGLSIADMCKHEAETATFARAIGYRAKPVIAAVEGYALGGGMILAASCDIVVAAS 121 DL EL L + +C E A F R I KP++AAV+GYA+GGG LAA+CD+V+AA Sbjct: 88 DLTELGALDLDGICDVEKRKAAFLRRISLCPKPIVAAVQGYAVGGGAFLAAACDVVIAAE 147 Query: 122 NARWHLPEAPNGWLPPWGLTALLTRVGPVRARRLTWGAASIDTIEAARIGLVDDVAEPGA 181 N ++ E PNGW+ PWG AL R+ P A+R WG + EA R GLVD+V Sbjct: 148 NVKFKGMEVPNGWITPWGFHALNARMAPRHAQRAAWGYKFMTATEALRTGLVDEVVALDD 207 Query: 182 ALSLATEIAVGLSKVPPEAVASTKRFFDRFIMADAERLDEVSSREFASNCETPSSQKTFE 241 A IA L+ +PP +V +TKR++ +AD E D + F +C+ ++Q+TF+ Sbjct: 208 LFETARTIAEQLAALPPVSVQATKRWYLDACLADGEAEDAKLNTLFRQHCQMEAAQETFK 267 Query: 242 RF 243 +F Sbjct: 268 KF 269