BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0118.0027.p (411 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001208269.1| glutamate carboxypeptidase [Bradyrhizobium s... 606 e-171 ref|YP_001237350.1| glutamate carboxypeptidase [Bradyrhizobium s... 596 e-168 ref|YP_002943477.1| glutamate carboxypeptidase [Variovorax parad... 515 e-144 ref|YP_004681265.1| carboxypeptidase G2 [Cupriavidus necator N-1... 514 e-144 ref|YP_840626.1| glutamate carboxypeptidase [Ralstonia eutropha ... 509 e-142 >ref|YP_001208269.1| glutamate carboxypeptidase [Bradyrhizobium sp. ORS 278] emb|CAL80054.1| Putative carboxypeptidase G2 precursor (Folate hydrolase G2) (Pteroylmonoglutamic acid hydrolase G2) (Glutamate carboxypeptidase); putative Peptidase family M20/M25/M40 [Bradyrhizobium sp. ORS 278] Length = 418 Score = 606 bits (1562), Expect = e-171, Method: Compositional matrix adjust. Identities = 300/416 (72%), Positives = 351/416 (84%), Gaps = 7/416 (1%) Query: 1 MKLSIKLLASAGLLALAS--SSAFAAADEKLRAX-----PAVIESLHDMVLIESGSSDVE 53 M S LA+ LLA ++ S+A+AA D+K+RA PAVI+SL +MVLIESGSSDVE Sbjct: 1 MTPSTSRLAALVLLASSATVSAAYAAPDDKIRAAAEQAKPAVIDSLREMVLIESGSSDVE 60 Query: 54 GLQKMADYTEARLKALGAKTERRKTTKGAGADMVIGSFEGTGTKKLMLIAHMDTVYEHGI 113 GL+KMAD TEARLKALGA TERRKTT+G GADMVIG+FEGTG +KLMLIAHMDTVY+ GI Sbjct: 61 GLKKMADLTEARLKALGATTERRKTTRGVGADMVIGTFEGTGRRKLMLIAHMDTVYQPGI 120 Query: 114 LDTQPYKIDGTRIYGPGIADDKGGIAVILHSLKILNDAGWRDYAKLTVSFNPDEEVGSIG 173 L T+PY ++G IYGPGIADDKGGIAVILHSL+IL D GWRDYA+LTV+FNPDEEVGSIG Sbjct: 121 LKTEPYHVEGDLIYGPGIADDKGGIAVILHSLQILKDMGWRDYARLTVAFNPDEEVGSIG 180 Query: 174 SGEIIAELADQHDVVLSFEPTVAPPVAKNESLLLGASGTATGTIDVKGKASHAGAAPQLG 233 SGE IAELADQHDVVLS EPT A P A +SLLLGASGTAT T++V G+A+HAGAAP+ G Sbjct: 181 SGEFIAELADQHDVVLSCEPTAAKPAAPVDSLLLGASGTATATMEVTGRAAHAGAAPERG 240 Query: 234 RNALIELAHQLLQTQDVAKSIPGTQLNWTTAKAGTVRNQIPDHATAGADIRLTIPDGVQK 293 RNALIEL+HQLLQT+D+AKSIPGTQLNWTTA AGTVRNQIP+ A+AG D+RLT+ DGV+K Sbjct: 241 RNALIELSHQLLQTRDIAKSIPGTQLNWTTATAGTVRNQIPEKASAGGDVRLTVADGVEK 300 Query: 294 LQAALDEKVKNRLIADTEVTVKVVAGRPPFVADDRGRALARQGQAIYAELGRTLDIAEMT 353 LQAALDE +K L+ DTEV V++ GRPPF+A +RGRAL ++ QAIYAE+ R L + +MT Sbjct: 301 LQAALDETIKTHLVPDTEVKVRIDKGRPPFIASERGRALGKEAQAIYAEMERPLALVDMT 360 Query: 354 GGATDAGYAARSGKAVVVESFGLAGWGYHARDEYIDTNSIVPRLYLMTRTLMELGK 409 GGATDAG A RSGKA+VVESFG+AG+ YHA +EYID +IVPRLYLMTR L+E GK Sbjct: 361 GGATDAGLANRSGKAIVVESFGIAGFNYHAHNEYIDATTIVPRLYLMTRMLIEQGK 416 >ref|YP_001237350.1| glutamate carboxypeptidase [Bradyrhizobium sp. BTAi1] gb|ABQ33444.1| putative carboxypeptidase G2 precursor [Bradyrhizobium sp. BTAi1] Length = 418 Score = 596 bits (1536), Expect = e-168, Method: Compositional matrix adjust. Identities = 292/396 (73%), Positives = 335/396 (84%), Gaps = 5/396 (1%) Query: 19 SSAFAAADEKLRAX-----PAVIESLHDMVLIESGSSDVEGLQKMADYTEARLKALGAKT 73 S+A A DE ++A PAVI+SL +MVLIESGSSDVEGL+KMAD TE RLKALGA T Sbjct: 21 SAAQAGPDETIKAAAEQAKPAVIDSLREMVLIESGSSDVEGLKKMADLTEGRLKALGATT 80 Query: 74 ERRKTTKGAGADMVIGSFEGTGTKKLMLIAHMDTVYEHGILDTQPYKIDGTRIYGPGIAD 133 ERRKTT+G GADMVIG+FEGTG +KLMLIAHMDTVY+ GIL T+PY ++G IYGPGIAD Sbjct: 81 ERRKTTRGVGADMVIGTFEGTGRRKLMLIAHMDTVYQPGILKTEPYHVEGDLIYGPGIAD 140 Query: 134 DKGGIAVILHSLKILNDAGWRDYAKLTVSFNPDEEVGSIGSGEIIAELADQHDVVLSFEP 193 DKGGIAVILHSL+IL D GWRDYA+LTV+FNPDEEVGSIGSGE IAELADQHDVVLS EP Sbjct: 141 DKGGIAVILHSLQILKDMGWRDYARLTVAFNPDEEVGSIGSGEFIAELADQHDVVLSCEP 200 Query: 194 TVAPPVAKNESLLLGASGTATGTIDVKGKASHAGAAPQLGRNALIELAHQLLQTQDVAKS 253 T A P A +SLLLGASGTAT T+ V G+ASHAGAAP GRNALIEL+HQLLQT+D+AKS Sbjct: 201 TAAKPAAPVDSLLLGASGTATATMQVTGRASHAGAAPDRGRNALIELSHQLLQTRDIAKS 260 Query: 254 IPGTQLNWTTAKAGTVRNQIPDHATAGADIRLTIPDGVQKLQAALDEKVKNRLIADTEVT 313 IPGTQLNWTTA AGTVRNQIP+ A+AG D+RLT+ DGV+KLQAALDE K+ L+ DTEV Sbjct: 261 IPGTQLNWTTATAGTVRNQIPEKASAGGDVRLTVADGVEKLQAALDETTKSHLVPDTEVK 320 Query: 314 VKVVAGRPPFVADDRGRALARQGQAIYAELGRTLDIAEMTGGATDAGYAARSGKAVVVES 373 V + GRPPF+A DRGRAL ++ Q IYAEL R L + +MTGGATDAG+A RSGKA+VVES Sbjct: 321 VTIEKGRPPFIASDRGRALGKEAQGIYAELERPLALVDMTGGATDAGFANRSGKAIVVES 380 Query: 374 FGLAGWGYHARDEYIDTNSIVPRLYLMTRTLMELGK 409 FG+AG+ YHA +EYID+ +IVPRLYLMTR L+E GK Sbjct: 381 FGIAGFNYHAHNEYIDSTTIVPRLYLMTRMLIEQGK 416 >ref|YP_002943477.1| glutamate carboxypeptidase [Variovorax paradoxus S110] gb|ACS18211.1| peptidase M20 [Variovorax paradoxus S110] Length = 422 Score = 515 bits (1327), Expect = e-144, Method: Compositional matrix adjust. Identities = 257/378 (67%), Positives = 304/378 (80%), Gaps = 1/378 (0%) Query: 33 PAVIESLHDMVLIESGSSDVEGLQKMADYTEARLKALGAKTERRKTTKGAGADMVIGSFE 92 PAVI++L +MVLIESGS +V+GL KMAD EARLKA G KTERRK T GAGAD+VIG+ + Sbjct: 44 PAVIDNLKEMVLIESGSLNVDGLLKMADVVEARLKAAGFKTERRKATAGAGADIVIGTLK 103 Query: 93 GTGTKKLMLIAHMDTVYEHGILDTQPYKIDGTRIYGPGIADDKGGIAVILHSLKILNDAG 152 GTG +++ML HMDTVY GIL++QPYK+DG RIYGPGIADDKGG+AV++ SLKIL DAG Sbjct: 104 GTGKRRIMLQGHMDTVYAAGILNSQPYKVDGNRIYGPGIADDKGGLAVMMASLKILADAG 163 Query: 153 WRDYAKLTVSFNPDEEVGSIGSGEIIAELADQHDVVLSFEPTVAPPVAKNESLLLGASGT 212 WRDY LTV NPDEEVGS+GSGE+IA +ADQHD VLSFEPT A VAK ESLLLGA+G Sbjct: 164 WRDYDTLTVLMNPDEEVGSVGSGELIATMADQHDTVLSFEPTAAKSVAKGESLLLGAAGI 223 Query: 213 ATGTIDVKGKASHAGAAPQLGRNALIELAHQLLQTQDVAKSIPGTQLNWTTAKAGTVRNQ 272 A T+DVKG+ASHAGAAP+LGRNAL EL++QLLQT+DVAK IPG LNWT A+A NQ Sbjct: 224 AQATLDVKGRASHAGAAPELGRNALYELSYQLLQTRDVAKDIPGVALNWTVARATGPINQ 283 Query: 273 IPDHATAGADIRLTIPDGVQKLQAALDEKVKN-RLIADTEVTVKVVAGRPPFVADDRGRA 331 I + A A DIR+T P +KL+AAL K+ + +L+ DTE T+KV GRP FVA ++GRA Sbjct: 284 ITEKAQALGDIRITQPGAEKKLEAALQAKLASGKLVPDTETTLKVEVGRPAFVAGEKGRA 343 Query: 332 LARQGQAIYAELGRTLDIAEMTGGATDAGYAARSGKAVVVESFGLAGWGYHARDEYIDTN 391 L + QAIY E+ R L + MTGG TDAGYA RSGKA VVESFGLAG+GYHARDEYI+ + Sbjct: 344 LGEKAQAIYKEIDRELALTPMTGGGTDAGYAGRSGKATVVESFGLAGFGYHARDEYIEVD 403 Query: 392 SIVPRLYLMTRTLMELGK 409 SIVPRLYL+TR L E+GK Sbjct: 404 SIVPRLYLVTRLLTEIGK 421 >ref|YP_004681265.1| carboxypeptidase G2 [Cupriavidus necator N-1] gb|AEI80033.1| carboxypeptidase G2 [Cupriavidus necator N-1] Length = 419 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 257/380 (67%), Positives = 304/380 (80%), Gaps = 3/380 (0%) Query: 33 PAVIESLHDMVLIESGSSDVEGLQKMADYTEARLKALGAKTERRKTTKGAGADMVIGSFE 92 PA+++SL +MV IESGS++ +GL +MA YTE RLKALGAK ER TKG G MV +F Sbjct: 41 PALVQSLKEMVTIESGSANAKGLAQMAGYTEKRLKALGAKVERMPVTKGPGT-MVKATFT 99 Query: 93 GTGTKKLMLIAHMDTVYEHGILDTQPYKIDGTRIYGPGIADDKGGIAVILHSLKILNDAG 152 GTG +++MLIAHMDTVY L TQP + +G R+YGPGIADDKGGIAVILHSL+IL + G Sbjct: 100 GTGKRRIMLIAHMDTVYPENTLATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQG 159 Query: 153 WRDYAKLTVSFNPDEEVGSIGSGEIIAELADQHDVVLSFEPTVAPPVAKNESLLLGASGT 212 WRDYA++TV FNPDEEVGS+GSGE IA LA QHDVVLS EPT A V K ESLLLGASGT Sbjct: 160 WRDYAQITVLFNPDEEVGSVGSGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGT 219 Query: 213 ATGTIDVKGKASHAGAAPQLGRNALIELAHQLLQTQDVAKSIPGTQLNWTTAKAGTVRNQ 272 AT T+ VKG+ASHAGAAP+ GRNALIELAHQL QT+D A +PG+QLNWT A+AGTVRNQ Sbjct: 220 ATATMQVKGRASHAGAAPERGRNALIELAHQLQQTRDTASQVPGSQLNWTQAQAGTVRNQ 279 Query: 273 IPDHATAGADIRLTIPDGVQKLQAALDEKVK-NRLIADTEVTVKVVAGRPPFVADDRGRA 331 IP+ A A D+R T +KL+ AL EK K ++L+ +T+ TV + GRPPFVAD RGRA Sbjct: 280 IPESAVAYGDVRTTAAGAAEKLKVALQEKAKSSQLVPETQTTVTMEEGRPPFVADARGRA 339 Query: 332 LARQGQAIYAEL-GRTLDIAEMTGGATDAGYAARSGKAVVVESFGLAGWGYHARDEYIDT 390 LA++ Q IYAEL GRTL +AE TGGATDA YAARSGK VVESFGLAG+GYHARDEYI+ Sbjct: 340 LAKRAQEIYAELDGRTLALAEGTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIEL 399 Query: 391 NSIVPRLYLMTRTLMELGKS 410 +SIVPRLYLMTR L ++G++ Sbjct: 400 DSIVPRLYLMTRILQDIGRN 419 >ref|YP_840626.1| glutamate carboxypeptidase [Ralstonia eutropha H16] emb|CAJ95896.1| putative peptidase, M20A subfamily [Ralstonia eutropha H16] Length = 419 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 255/380 (67%), Positives = 305/380 (80%), Gaps = 3/380 (0%) Query: 33 PAVIESLHDMVLIESGSSDVEGLQKMADYTEARLKALGAKTERRKTTKGAGADMVIGSFE 92 PA+++SL +MV IESGS++ +GL +MA YTE RL+ALGAK ER TKG G MV +F Sbjct: 41 PALVQSLKEMVSIESGSANAKGLAQMAGYTEKRLQALGAKVERMPVTKGPGT-MVKATFT 99 Query: 93 GTGTKKLMLIAHMDTVYEHGILDTQPYKIDGTRIYGPGIADDKGGIAVILHSLKILNDAG 152 GTG +++MLIAHMDTVY L TQP + +G R+YGPGIADDKGGIAVILHSL+IL + G Sbjct: 100 GTGKRRIMLIAHMDTVYPDNTLATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQG 159 Query: 153 WRDYAKLTVSFNPDEEVGSIGSGEIIAELADQHDVVLSFEPTVAPPVAKNESLLLGASGT 212 WRDYA++TV FNPDEEVGS+GSGE IA LA QHDVVLS EPT A V K ESLLLGASGT Sbjct: 160 WRDYAQITVLFNPDEEVGSVGSGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGT 219 Query: 213 ATGTIDVKGKASHAGAAPQLGRNALIELAHQLLQTQDVAKSIPGTQLNWTTAKAGTVRNQ 272 AT T+ VKG+ASHAGAAP+ GRNAL+ELAHQL QT+D A +PG+QLNWT A+AGTVRNQ Sbjct: 220 ATATMQVKGRASHAGAAPERGRNALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQ 279 Query: 273 IPDHATAGADIRLTIPDGVQKLQAALDEKVKN-RLIADTEVTVKVVAGRPPFVADDRGRA 331 IP+ A A D+R T +KL+ AL +KVK+ +L+ +T+ TV + GRPPFVAD RGRA Sbjct: 280 IPESAVAYGDVRTTAAGAAEKLKVALQDKVKSGQLVPETQTTVTMEEGRPPFVADARGRA 339 Query: 332 LARQGQAIYAEL-GRTLDIAEMTGGATDAGYAARSGKAVVVESFGLAGWGYHARDEYIDT 390 LA++ Q IYAEL GRTL +AE TGGATDA YAARSGK VVESFGLAG+GYHARDEYI+ Sbjct: 340 LAKRAQEIYAELDGRTLALAEGTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIEL 399 Query: 391 NSIVPRLYLMTRTLMELGKS 410 +SIVPRLYLMTR L ++G++ Sbjct: 400 DSIVPRLYLMTRILQDIGRN 419