BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0118.0004.p (473 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_769869.1| glutamyl-tRNA(Gln) amidotransferase subunit [Br... 759 0.0 ref|YP_726351.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A ... 711 0.0 ref|YP_004685685.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase... 707 0.0 ref|YP_583918.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase su... 698 0.0 ref|YP_004685625.1| amidase [Cupriavidus necator N-1] >gi|338166... 605 e-171 >ref|NP_769869.1| glutamyl-tRNA(Gln) amidotransferase subunit [Bradyrhizobium japonicum USDA 110] dbj|BAC48494.1| blr3229 [Bradyrhizobium japonicum USDA 110] Length = 475 Score = 759 bits (1961), Expect = 0.0, Method: Compositional matrix adjust. Identities = 389/472 (82%), Positives = 429/472 (90%) Query: 1 MSDDLCLLSAVELRGRIARKEVSPVEVTRAVLARAERLQPELNCFITLCGDAAMAQAKAA 60 M+ D CLLSA ELRG IA K +SPVE+TRAVLARAE LQP+LNCFIT+CGD AMA A+AA Sbjct: 3 MNGDPCLLSATELRGLIADKRISPVEITRAVLARAEALQPQLNCFITICGDEAMAAARAA 62 Query: 61 ERQITAGEPLGLLHGIPFTVKDIVNTKDIRTTFGAVPYRDNVPDNDAVAVARLRAQGGIL 120 ER++ AGEPLGLLHGIP+TVKDIVNT+ ++TTFGAVPY+DNVP DAVAVARLR++G IL Sbjct: 63 ERRVMAGEPLGLLHGIPYTVKDIVNTRGVKTTFGAVPYKDNVPVEDAVAVARLRSEGAIL 122 Query: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAERSSGGSSGGAAVAVSSGIAPLAVATDGGGS 180 IGKTTTPEFGSKCLTDSPLFGRTRNAW A RSSGGSSGGAAVAV+SGIAPLAVATDGGGS Sbjct: 123 IGKTTTPEFGSKCLTDSPLFGRTRNAWDACRSSGGSSGGAAVAVASGIAPLAVATDGGGS 182 Query: 181 TRIPAACNGVVGIKQSNGVIPHSQVQDAFGNQTYVTPTTRTVADTALMMQAMAGEDPSDP 240 TRIPAACNGVVG+KQSNG+IPHSQ D FGNQTYVTPTTRTVADTALMMQAMAGEDP DP Sbjct: 183 TRIPAACNGVVGLKQSNGIIPHSQALDVFGNQTYVTPTTRTVADTALMMQAMAGEDPCDP 242 Query: 241 WSIGVPAPDYLGLAAPRGDLKGRRVLFCVSPPGRPVTSDVAAAFKASLDRLASLGAELEE 300 WSIGVP D++G AAPRGDL+G R+L+C++PPGRPV+++VAA+F+ASLDRLA LGAELEE Sbjct: 243 WSIGVPVSDFVGTAAPRGDLRGLRILYCLTPPGRPVSAEVAASFRASLDRLAGLGAELEE 302 Query: 301 FSGDGFDIEPIWRAINHTVWRTRFEKLAADHQNDLSATFLKQLALAAQVSGVDYQRAMFE 360 FSG+GFDIEPIWRAINHTVWRTRF KLAA+H+++LS FLKQLALA +VSGVDYQ AMF Sbjct: 303 FSGEGFDIEPIWRAINHTVWRTRFAKLAAEHKDELSEAFLKQLALATEVSGVDYQEAMFA 362 Query: 361 RTALFRRVQSLFARGDLLAMPTLTRSALPIDQDLFGTIEIDGRSFDSVRPHWFPWTMLFN 420 RTALFRRVQSL ARG LAMPTLTRSALPI QDLFGTIEIDGR FDSVRPHWFPWTM FN Sbjct: 363 RTALFRRVQSLLARGHFLAMPTLTRSALPIKQDLFGTIEIDGRHFDSVRPHWFPWTMPFN 422 Query: 421 MTGHPAVSIPAGFGRDALPIGLHLAGRFRADAELLRVAALFEAASDLLGRWP 472 MTGHPAVS+P+GFGRD LPIGL L GRFRADAELLRV+ LFEA+ DLL RWP Sbjct: 423 MTGHPAVSLPSGFGRDGLPIGLQLVGRFRADAELLRVSTLFEASVDLLSRWP 474 >ref|YP_726351.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit or related amidase [Ralstonia eutropha H16] emb|CAJ92983.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit or related amidase [Ralstonia eutropha H16] Length = 475 Score = 711 bits (1834), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/472 (73%), Positives = 402/472 (85%) Query: 1 MSDDLCLLSAVELRGRIARKEVSPVEVTRAVLARAERLQPELNCFITLCGDAAMAQAKAA 60 MS+ LCL SA EL+ RI RKE+SPVE+T AVLARAERLQP LNCFITLC D AMA A+AA Sbjct: 1 MSEALCLASAAELQQRIRRKEISPVELTAAVLARAERLQPVLNCFITLCADEAMAAARAA 60 Query: 61 ERQITAGEPLGLLHGIPFTVKDIVNTKDIRTTFGAVPYRDNVPDNDAVAVARLRAQGGIL 120 E ++ +G PL LHGIPFT KDIVNT+ +RTTFGAVP RDNVP DAVAVARLRAQG IL Sbjct: 61 EAEVMSGRPLPPLHGIPFTAKDIVNTRGVRTTFGAVPMRDNVPAEDAVAVARLRAQGAIL 120 Query: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAERSSGGSSGGAAVAVSSGIAPLAVATDGGGS 180 +GKTTTPEFGSK LTDSPLFGRTRNAW AER+SGGSSGGAAVAV+SG+APLAVATDGGGS Sbjct: 121 VGKTTTPEFGSKSLTDSPLFGRTRNAWHAERTSGGSSGGAAVAVASGMAPLAVATDGGGS 180 Query: 181 TRIPAACNGVVGIKQSNGVIPHSQVQDAFGNQTYVTPTTRTVADTALMMQAMAGEDPSDP 240 TRIPAACNGVVGIKQS GVIPHSQ QD FGNQTYVTPTTRTVADT LM+QAMAGE P DP Sbjct: 181 TRIPAACNGVVGIKQSQGVIPHSQAQDLFGNQTYVTPTTRTVADTGLMLQAMAGEHPCDP 240 Query: 241 WSIGVPAPDYLGLAAPRGDLKGRRVLFCVSPPGRPVTSDVAAAFKASLDRLASLGAELEE 300 WSIGVPA DY+ A P+GDL+GR +L+C++PPGRPV++DVA AF+ASLD+L +LGAELE Sbjct: 241 WSIGVPAADYVAAAQPQGDLRGRCILYCLAPPGRPVSADVARAFEASLDKLRALGAELEP 300 Query: 301 FSGDGFDIEPIWRAINHTVWRTRFEKLAADHQNDLSATFLKQLALAAQVSGVDYQRAMFE 360 F G+GFD+EP+WRAINHTVWRTRF + A H+ LS TF++Q+ AA + V+YQ+AMFE Sbjct: 301 FGGEGFDVEPVWRAINHTVWRTRFAPIVAQHREALSPTFVRQVESAAAFTAVEYQQAMFE 360 Query: 361 RTALFRRVQSLFARGDLLAMPTLTRSALPIDQDLFGTIEIDGRSFDSVRPHWFPWTMLFN 420 R+ LF RVQ+L R DLLAMPT+TR+ALP++QDLFG IEIDG+ + VR +WFPWTM FN Sbjct: 361 RSQLFLRVQALLGRADLLAMPTITRTALPLEQDLFGQIEIDGQDYPDVRANWFPWTMPFN 420 Query: 421 MTGHPAVSIPAGFGRDALPIGLHLAGRFRADAELLRVAALFEAASDLLGRWP 472 MTGHPA+S+P GFG D LPIGL L G+FR DA+LLRVAAL+E+ DL G+WP Sbjct: 421 MTGHPAISLPCGFGGDGLPIGLQLVGQFRGDAQLLRVAALYESVHDLTGQWP 472 >ref|YP_004685685.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit [Cupriavidus necator N-1] gb|AEI77204.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit [Cupriavidus necator N-1] Length = 483 Score = 707 bits (1825), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/472 (73%), Positives = 399/472 (84%) Query: 1 MSDDLCLLSAVELRGRIARKEVSPVEVTRAVLARAERLQPELNCFITLCGDAAMAQAKAA 60 MS+ LCL SA EL+ RI RKE+SPVE+T AVLARAERLQP LNCFITLC D AMA A+AA Sbjct: 1 MSEALCLASAAELQQRIRRKEISPVELTAAVLARAERLQPVLNCFITLCADEAMAAARAA 60 Query: 61 ERQITAGEPLGLLHGIPFTVKDIVNTKDIRTTFGAVPYRDNVPDNDAVAVARLRAQGGIL 120 E ++ +G PL LHGIPFT KDIVNT+ +RTTFGAVP RDNVP DAVAVARLRAQG IL Sbjct: 61 EAEVMSGRPLPPLHGIPFTAKDIVNTRGVRTTFGAVPMRDNVPAEDAVAVARLRAQGAIL 120 Query: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAERSSGGSSGGAAVAVSSGIAPLAVATDGGGS 180 +GKTTTPEFGSKCLTDSPLFGRTRNAW A R+SGGSSGGAAVAV+SGIAPLAVATDGGGS Sbjct: 121 VGKTTTPEFGSKCLTDSPLFGRTRNAWHAARTSGGSSGGAAVAVASGIAPLAVATDGGGS 180 Query: 181 TRIPAACNGVVGIKQSNGVIPHSQVQDAFGNQTYVTPTTRTVADTALMMQAMAGEDPSDP 240 TRIPAACNGVVGIKQS GVIPHSQ QD FGNQTYVTPTTRTVADT LM+QAMAGE DP Sbjct: 181 TRIPAACNGVVGIKQSQGVIPHSQAQDLFGNQTYVTPTTRTVADTGLMLQAMAGEHACDP 240 Query: 241 WSIGVPAPDYLGLAAPRGDLKGRRVLFCVSPPGRPVTSDVAAAFKASLDRLASLGAELEE 300 WSIGVPA DY+ A P+GDL+GRR+L+C++ PGRPV++DVA AF+ASLD+L +LGAEL Sbjct: 241 WSIGVPAADYVAAAQPQGDLRGRRILYCLATPGRPVSADVARAFEASLDKLRALGAELVP 300 Query: 301 FSGDGFDIEPIWRAINHTVWRTRFEKLAADHQNDLSATFLKQLALAAQVSGVDYQRAMFE 360 F G+GFD+EPIWRAINHTVWRTRF + A H+ LS TF++Q+ AA + V+YQ+AMFE Sbjct: 301 FGGEGFDVEPIWRAINHTVWRTRFAPIVAQHREALSPTFVRQVESAAAFTAVEYQQAMFE 360 Query: 361 RTALFRRVQSLFARGDLLAMPTLTRSALPIDQDLFGTIEIDGRSFDSVRPHWFPWTMLFN 420 R+ LF RVQ+L R DLLAMPT+TR+ALP++QDLFG IEIDG+ + VR +WFPWTM FN Sbjct: 361 RSQLFLRVQALLGRADLLAMPTITRTALPLEQDLFGQIEIDGQDYPDVRANWFPWTMPFN 420 Query: 421 MTGHPAVSIPAGFGRDALPIGLHLAGRFRADAELLRVAALFEAASDLLGRWP 472 MTGHPA+S+P GFG D LPIGL L G+FR DA+LLR AAL+E+ DL G+WP Sbjct: 421 MTGHPAISLPCGFGSDGLPIGLQLVGQFRGDAQLLRAAALYESVHDLTGQWP 472 >ref|YP_583918.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase subunit A-like protein [Cupriavidus metallidurans CH34] gb|ABF08649.1| Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit or related amidase [Cupriavidus metallidurans CH34] Length = 475 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/474 (73%), Positives = 396/474 (83%), Gaps = 1/474 (0%) Query: 1 MSDDLCLLSAVELRGRIARKEVSPVEVTRAVLARAERLQPELNCFITLCGDAAMAQAKAA 60 MS+ LC LSA EL I K VSPV++T AVLARAERLQPELNCFIT+ + AM A+ A Sbjct: 1 MSESLCFLSATELADAIRHKRVSPVDITSAVLARAERLQPELNCFITIDAERAMESARQA 60 Query: 61 ERQITAGEPLGLLHGIPFTVKDIVNTKDIRTTFGAVPYRDNVPDNDAVAVARLRAQGGIL 120 ER + AG PLGLLHG+PFTVKDIVNT+ +RTTFGA P RDNVP DAVAVARLR QG IL Sbjct: 61 ERLVMAGGPLGLLHGVPFTVKDIVNTQGVRTTFGAAPLRDNVPQQDAVAVARLREQGAIL 120 Query: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAERSSGGSSGGAAVAVSSGIAPLAVATDGGGS 180 IGKTTTPEFGSKCLTDSPLFGRTRNAWSA+R+SGGSSGGAAVAV+SGIAPLAVATDGGGS Sbjct: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAQRTSGGSSGGAAVAVASGIAPLAVATDGGGS 180 Query: 181 TRIPAACNGVVGIKQSNGVIPHSQVQDAFGNQTYVTPTTRTVADTALMMQAMAGEDPSDP 240 TRIPAACNGVVGIKQSNGVIPHSQ QD +GNQTYVTPTTRTVADT LMM AMAGE DP Sbjct: 181 TRIPAACNGVVGIKQSNGVIPHSQAQDLYGNQTYVTPTTRTVADTGLMMAAMAGEHACDP 240 Query: 241 WSIGVPAPDYLGLAAPRGDLKGRRVLFCVSPPGRPVTSDVAAAFKASLDRLASLGAELEE 300 WSIGVP DY+ A +GDL+G+R+L+C++PPGRPV +DVA AF+ L LA LGAELE Sbjct: 241 WSIGVPKADYVDAARAQGDLRGKRILYCLAPPGRPVAADVARAFERGLAALAGLGAELEP 300 Query: 301 FSGDGF-DIEPIWRAINHTVWRTRFEKLAADHQNDLSATFLKQLALAAQVSGVDYQRAMF 359 F GDGF DIEP+WRAINHTVWRTRF ++ H+++LSATF++Q+ AAQVSGV YQ+AMF Sbjct: 301 FDGDGFNDIEPLWRAINHTVWRTRFVEIVERHRDELSATFVRQVESAAQVSGVAYQQAMF 360 Query: 360 ERTALFRRVQSLFARGDLLAMPTLTRSALPIDQDLFGTIEIDGRSFDSVRPHWFPWTMLF 419 +R+ LF+RVQ+L R DLLAMPT+ R+ALPIDQDLFGTIEIDG ++VR +WFPWTM F Sbjct: 361 DRSRLFQRVQALLERADLLAMPTIMRTALPIDQDLFGTIEIDGHVHENVRANWFPWTMPF 420 Query: 420 NMTGHPAVSIPAGFGRDALPIGLHLAGRFRADAELLRVAALFEAASDLLGRWPA 473 NMTGHPA+S+P GFG D LPIGL L GRFR D ELLR AALFE+A L RWP+ Sbjct: 421 NMTGHPAISLPCGFGSDDLPIGLQLVGRFRRDTELLRCAALFESAHGFLDRWPS 474 >ref|YP_004685625.1| amidase [Cupriavidus necator N-1] gb|AEI77144.1| amidase [Cupriavidus necator N-1] Length = 477 Score = 605 bits (1559), Expect = e-171, Method: Compositional matrix adjust. Identities = 312/472 (66%), Positives = 373/472 (79%) Query: 1 MSDDLCLLSAVELRGRIARKEVSPVEVTRAVLARAERLQPELNCFITLCGDAAMAQAKAA 60 M+++LC L AV LR I +EVSPVEVTRAVL RA RLQ LNCFITLC D AM +A+AA Sbjct: 1 MNEELCFLPAVRLREAITAREVSPVEVTRAVLDRATRLQSTLNCFITLCPDEAMREARAA 60 Query: 61 ERQITAGEPLGLLHGIPFTVKDIVNTKDIRTTFGAVPYRDNVPDNDAVAVARLRAQGGIL 120 E+ + GEP GLLHGIP+ KD+VNT+ + TTFG++ + DNVP DAVAVARLR+QG IL Sbjct: 61 EQALMRGEPAGLLHGIPYACKDLVNTRGVLTTFGSLLFADNVPTEDAVAVARLRSQGAIL 120 Query: 121 IGKTTTPEFGSKCLTDSPLFGRTRNAWSAERSSGGSSGGAAVAVSSGIAPLAVATDGGGS 180 IGKTTT EFG+KCLTD+PLFGRTRNAW +R+SGGSSGGAAVAV++G+AP+ +ATDGGGS Sbjct: 121 IGKTTTSEFGAKCLTDAPLFGRTRNAWHPDRTSGGSSGGAAVAVAAGLAPIGIATDGGGS 180 Query: 181 TRIPAACNGVVGIKQSNGVIPHSQVQDAFGNQTYVTPTTRTVADTALMMQAMAGEDPSDP 240 TRIPAACNGVVG+KQS G++PHSQV+D FGN TYVTP +RTVADTALM+QAMAGED SDP Sbjct: 181 TRIPAACNGVVGLKQSIGLVPHSQVEDVFGNLTYVTPISRTVADTALMLQAMAGEDASDP 240 Query: 241 WSIGVPAPDYLGLAAPRGDLKGRRVLFCVSPPGRPVTSDVAAAFKASLDRLASLGAELEE 300 WS GV A DYLG DL+ +R+LF PPGRP+ +DVA F ++L+RL LGAEL E Sbjct: 241 WSAGVRATDYLGAMRAVTDLRRKRILFASVPPGRPLAADVAKCFGSALERLQDLGAELAE 300 Query: 301 FSGDGFDIEPIWRAINHTVWRTRFEKLAADHQNDLSATFLKQLALAAQVSGVDYQRAMFE 360 G GF+IEP+WR INHTVWR RF ++A + LS + L+QLALA S VDYQRA FE Sbjct: 301 MPGSGFNIEPLWRTINHTVWRARFGQMAREQPERLSPSLLQQLALANDYSAVDYQRANFE 360 Query: 361 RTALFRRVQSLFARGDLLAMPTLTRSALPIDQDLFGTIEIDGRSFDSVRPHWFPWTMLFN 420 RT LFR VQ+L D +A+PT+ R+ALPIDQDLFG IEIDGR +D VR +WFPWTM FN Sbjct: 361 RTRLFRHVQALLRDHDFIAVPTIARTALPIDQDLFGQIEIDGRLYDEVRSNWFPWTMPFN 420 Query: 421 MTGHPAVSIPAGFGRDALPIGLHLAGRFRADAELLRVAALFEAASDLLGRWP 472 +TGHPA+++P GFGR LPIGL L GR R ++ELL AAL EA+ +LL RWP Sbjct: 421 LTGHPAITLPCGFGRGGLPIGLQLVGRLRGESELLGAAALLEASQNLLSRWP 472