BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0117.0036.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774120.1| riboflavin biosynthesis bifunctional protein [B... 550 e-154 ref|YP_319146.1| riboflavin kinase / FAD synthetase [Nitrobacter... 538 e-151 ref|YP_578356.1| riboflavin biosynthesis protein RibF [Nitrobact... 533 e-149 ref|YP_001237832.1| FMN adenylyltransferase / riboflavin kinase ... 516 e-144 ref|YP_001207934.1| riboflavin biosynthesis protein ribF/bifunct... 498 e-139 >ref|NP_774120.1| riboflavin biosynthesis bifunctional protein [Bradyrhizobium japonicum USDA 110] dbj|BAC52745.1| riboflavin biosynthesis bifunctional protein [Bradyrhizobium japonicum USDA 110] Length = 380 Score = 550 bits (1416), Expect = e-154, Method: Compositional matrix adjust. Identities = 276/322 (85%), Positives = 299/322 (92%) Query: 1 MSTGFTVIRDNTPASAIPKGAVVAMGNFDGVHLGHRAVIGAALAMGRAHGVPALAVTFEP 60 M+ FTVIRD TP SAIP+GAVVAMGNFDGVHLGHRAVI AAL MGRAHG PALA+TFEP Sbjct: 58 MAPHFTVIRDTTPDSAIPRGAVVAMGNFDGVHLGHRAVIAAALEMGRAHGRPALALTFEP 117 Query: 61 HPRSFFSPNTPQFRLTDETNKLRLLAATGLAGAVVMTFDKARAGTSAQDFIHHDLIGRLG 120 HPR FFSPNTPQFRLTDE KLRLLA TGL+GAVVMTFDKARAGTSAQDFIHHDLIGRLG Sbjct: 118 HPRRFFSPNTPQFRLTDERAKLRLLAGTGLSGAVVMTFDKARAGTSAQDFIHHDLIGRLG 177 Query: 121 ISGIAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHVDIEERPVSSSAIRMALAEGE 180 +S IAVGYDFHFGKGRVGSPSLLV+EAPRLGIEVDVQ HVD +ERPVSSSAIR+ALAEG+ Sbjct: 178 VSAIAVGYDFHFGKGRVGSPSLLVNEAPRLGIEVDVQPHVDFDERPVSSSAIRIALAEGQ 237 Query: 181 IADATTMLGGPWFVTGKVIHGEKRGRDLGYPTANIRLDKHCGLKHGIYAVRVGKGSQRFD 240 + +ATTMLG PWF+TG+VIHGEKRGRDLGYPTANIRLD +CGLKHGIYAVRVG+G++R D Sbjct: 238 LDEATTMLGAPWFITGEVIHGEKRGRDLGYPTANIRLDANCGLKHGIYAVRVGRGAERLD 297 Query: 241 GVASFGRRPTFDNGAPLLEAFLFDFKGDLYDAVLDVAFIGFIREELKFDTIEALIRQMDD 300 GVASFGRRPTFDNGAPLLE FLFDFKGDLY LD AF+GFIREELKFD++EALIRQMDD Sbjct: 298 GVASFGRRPTFDNGAPLLEIFLFDFKGDLYGQALDCAFVGFIREELKFDSLEALIRQMDD 357 Query: 301 DSAKARAVLAAAPDAFPQLGVI 322 DSA+ARA+LAAAPDAFP+LGVI Sbjct: 358 DSARARAMLAAAPDAFPRLGVI 379 >ref|YP_319146.1| riboflavin kinase / FAD synthetase [Nitrobacter winogradskyi Nb-255] gb|ABA05794.1| FMN adenylyltransferase [Nitrobacter winogradskyi Nb-255] Length = 327 Score = 538 bits (1386), Expect = e-151, Method: Compositional matrix adjust. Identities = 267/327 (81%), Positives = 287/327 (87%), Gaps = 4/327 (1%) Query: 1 MSTGFTVIRDNTPASAIPKGAVVAMGNFDGVHLGHRAVIGAALAMGRAHGVPALAVTFEP 60 M++GFT+IRD PA IP+G VVAMGNFDGVHLGHRAVI AAL M R PALA+TFEP Sbjct: 1 MTSGFTIIRDTAPADVIPRGGVVAMGNFDGVHLGHRAVIAAALRMARLRAKPALAMTFEP 60 Query: 61 HPRSFFSPNTPQFRLTDETNKLRLLAATGLAGAVVMTFDKARAGTSAQDFIHHDLIGRLG 120 HPR FFSPNTPQFRLTDET KLRLLA TGLAGAVVMTFD RAGT+AQDFIHHDLI RLG Sbjct: 61 HPRKFFSPNTPQFRLTDETGKLRLLAGTGLAGAVVMTFDATRAGTTAQDFIHHDLIERLG 120 Query: 121 ISGIAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHVDIEERPVSSSAIRMALAEGE 180 +S +AVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQ HVD EERPVSSSAIRMALAE + Sbjct: 121 VSAVAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQPHVDFEERPVSSSAIRMALAEAQ 180 Query: 181 IADATTMLGGPWFVTGKVIHGEKRGRDLGYPTANIRLDKHCGLKHGIYAVRVGKGS---- 236 IA+AT MLGGPWFVTG+VIHGEKRGR LGYPTANI LD+ CGL+HGIYAVRVG G Sbjct: 181 IANATAMLGGPWFVTGEVIHGEKRGRGLGYPTANIHLDRDCGLRHGIYAVRVGHGKGKDH 240 Query: 237 QRFDGVASFGRRPTFDNGAPLLEAFLFDFKGDLYDAVLDVAFIGFIREELKFDTIEALIR 296 +RFDGVASFGRRPTFDNGAPLLE FLFDF+GDLY A+LDVAFIGFIR+ELKFD EAL+R Sbjct: 241 RRFDGVASFGRRPTFDNGAPLLEVFLFDFQGDLYGAILDVAFIGFIRDELKFDGAEALVR 300 Query: 297 QMDDDSAKARAVLAAAPDAFPQLGVIG 323 QMDDD+A AR LAAAPDAFP+LG IG Sbjct: 301 QMDDDAACARIQLAAAPDAFPKLGEIG 327 >ref|YP_578356.1| riboflavin biosynthesis protein RibF [Nitrobacter hamburgensis X14] gb|ABE63896.1| FMN adenylyltransferase [Nitrobacter hamburgensis X14] Length = 327 Score = 533 bits (1373), Expect = e-149, Method: Compositional matrix adjust. Identities = 276/326 (84%), Positives = 293/326 (89%), Gaps = 4/326 (1%) Query: 1 MSTGFTVIRDNTPASAIPKGAVVAMGNFDGVHLGHRAVIGAALAMGRAHGVPALAVTFEP 60 M FT+IRD TPA+AIPKG+VVAMGNFDGVHLGHRAVI AALAM R+ G PA AVTFEP Sbjct: 1 MPPRFTIIRDTTPAAAIPKGSVVAMGNFDGVHLGHRAVIEAALAMSRSRGKPAFAVTFEP 60 Query: 61 HPRSFFSPNTPQFRLTDETNKLRLLAATGLAGAVVMTFDKARAGTSAQDFIHHDLIGRLG 120 HPR FFSPNTPQFRLTDET+KLRLLA GLAGAVVMTFD ARAGT+AQDFIHHDLI RL Sbjct: 61 HPRKFFSPNTPQFRLTDETSKLRLLAGAGLAGAVVMTFDTARAGTTAQDFIHHDLIARLD 120 Query: 121 ISGIAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHVDIEERPVSSSAIRMALAEGE 180 +SGIAVGYDFHFGKGRVGSPSLL+SEA RLGIEVDV+ VDI+ERPVSSSAIRMALAEG+ Sbjct: 121 VSGIAVGYDFHFGKGRVGSPSLLLSEATRLGIEVDVRPPVDIDERPVSSSAIRMALAEGQ 180 Query: 181 IADATTMLGGPWFVTGKVIHGEKRGRDLGYPTANIRLDKHCGLKHGIYAVRVGKGS---- 236 IADAT MLGGPWFVTG+VIHGEKRGR LGYPTANIRLDK CGLKHGIYAVRVG G Sbjct: 181 IADATAMLGGPWFVTGEVIHGEKRGRHLGYPTANIRLDKDCGLKHGIYAVRVGLGQGKDH 240 Query: 237 QRFDGVASFGRRPTFDNGAPLLEAFLFDFKGDLYDAVLDVAFIGFIREELKFDTIEALIR 296 +RFDGVASFGRRPTFDNGAPLLE FLFDFKGDLY VLDVAFIGFIR+ELKFD+I+ALIR Sbjct: 241 RRFDGVASFGRRPTFDNGAPLLEVFLFDFKGDLYGTVLDVAFIGFIRDELKFDSIDALIR 300 Query: 297 QMDDDSAKARAVLAAAPDAFPQLGVI 322 QMDDDSA+AR LAAAPDAFP LGVI Sbjct: 301 QMDDDSARARTQLAAAPDAFPNLGVI 326 >ref|YP_001237832.1| FMN adenylyltransferase / riboflavin kinase [Bradyrhizobium sp. BTAi1] gb|ABQ33926.1| FMN adenylyltransferase [Bradyrhizobium sp. BTAi1] Length = 326 Score = 516 bits (1329), Expect = e-144, Method: Compositional matrix adjust. Identities = 256/322 (79%), Positives = 283/322 (87%), Gaps = 4/322 (1%) Query: 5 FTVIRDNTPASAIPKGAVVAMGNFDGVHLGHRAVIGAALAMGRAHGVPALAVTFEPHPRS 64 FT+IRD TP + I KGAVVAMGNFDGVHLGHRAVI AA+ MG++HG PALA+TFEPHPR Sbjct: 4 FTIIRDTTPPADIIKGAVVAMGNFDGVHLGHRAVIEAAVEMGKSHGRPALALTFEPHPRR 63 Query: 65 FFSPNTPQFRLTDETNKLRLLAATGLAGAVVMTFDKARAGTSAQDFIHHDLIGRLGISGI 124 FFSPNTPQFRLTDE KLRLLA TGL GAVVMTFDK RA TSAQDFIHH+LIGRLG++GI Sbjct: 64 FFSPNTPQFRLTDERAKLRLLAGTGLQGAVVMTFDKGRAQTSAQDFIHHELIGRLGVAGI 123 Query: 125 AVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHVDIEERPVSSSAIRMALAEGEIADA 184 AVGYDFHFGKGRVGSP+LL SEAP+LGIEVDVQ HVDI+ERPVSS+AIR ALAEG++A+A Sbjct: 124 AVGYDFHFGKGRVGSPTLLASEAPKLGIEVDVQPHVDIDERPVSSTAIREALAEGQVAEA 183 Query: 185 TTMLGGPWFVTGKVIHGEKRGRDLGYPTANIRLDKHCGLKHGIYAVRV--GKGSQR--FD 240 T MLGGPWFVT +VIHGEKRGRDLGYPTAN+RLD+ CGL+HGIYAVRV G+G+ R D Sbjct: 184 TAMLGGPWFVTSEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRVLID 243 Query: 241 GVASFGRRPTFDNGAPLLEAFLFDFKGDLYDAVLDVAFIGFIREELKFDTIEALIRQMDD 300 GVA+FGRRPTFDNGAPLLE FLFDFK LY VLD+AFIGFIREE KF +IEALIRQMDD Sbjct: 244 GVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDIAFIGFIREEAKFSSIEALIRQMDD 303 Query: 301 DSAKARAVLAAAPDAFPQLGVI 322 DSAKARA+L A FP+LG I Sbjct: 304 DSAKARALLQANAGLFPRLGEI 325 >ref|YP_001207934.1| riboflavin biosynthesis protein ribF/bifunctional flavokinase/FAD synthetase [Bradyrhizobium sp. ORS 278] emb|CAL79719.1| riboflavin biosynthesis protein ribF; bifunctional : flavokinase; FAD synthetase [Bradyrhizobium sp. ORS 278] Length = 326 Score = 498 bits (1282), Expect = e-139, Method: Compositional matrix adjust. Identities = 259/323 (80%), Positives = 281/323 (86%), Gaps = 4/323 (1%) Query: 5 FTVIRDNTPASAIPKGAVVAMGNFDGVHLGHRAVIGAALAMGRAHGVPALAVTFEPHPRS 64 F +IRD TP I KGAVVAMGNFDGVHLGHRAVI AA+ MGRA G PALA+TFEPHPR Sbjct: 4 FQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPHPRR 63 Query: 65 FFSPNTPQFRLTDETNKLRLLAATGLAGAVVMTFDKARAGTSAQDFIHHDLIGRLGISGI 124 FFSPNTPQFRLTDE KLRLLA GLAGAVVMTFDK RA TSAQDFIHHDLIGRLG+SGI Sbjct: 64 FFSPNTPQFRLTDEAAKLRLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGVSGI 123 Query: 125 AVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHVDIEERPVSSSAIRMALAEGEIADA 184 AVGYDFHFGKGRVGSPSLL SEAP+LGI V+VQ HVDI+ERPVSS+AIR ALAEG++A+A Sbjct: 124 AVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVDIDERPVSSTAIREALAEGQVAEA 183 Query: 185 TTMLGGPWFVTGKVIHGEKRGRDLGYPTANIRLDKHCGLKHGIYAVRV--GKGSQR--FD 240 T MLGGPWFVT +VIHGEKRGRDLGYPTAN+RLD+ CGL+HGIYAVRV G+G+ R D Sbjct: 184 TAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRVLID 243 Query: 241 GVASFGRRPTFDNGAPLLEAFLFDFKGDLYDAVLDVAFIGFIREELKFDTIEALIRQMDD 300 GVA+FGRRPTFDNGAPLLE FLFDFK LY VLDVAFIGFIREE +F +IEALIRQMDD Sbjct: 244 GVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQMDD 303 Query: 301 DSAKARAVLAAAPDAFPQLGVIG 323 DSA+ARAVLAA FP+LG IG Sbjct: 304 DSARARAVLAANAGLFPRLGEIG 326