BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0038.p (397 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_774243.1| benzoylformate decarboxylase [Bradyrhizobium ja... 606 e-171 ref|YP_001237761.1| hypothetical protein BBta_1644 [Bradyrhizobi... 573 e-161 ref|YP_001208015.1| putative thiamine pyrophosphate-requiring en... 538 e-151 ref|ZP_00052667.2| COG0028: Thiamine pyrophosphate-requiring enz... 246 7e-63 ref|YP_003068764.1| decarboxylase, Thiamine pyrophosphate-requir... 237 3e-60 >ref|NP_774243.1| benzoylformate decarboxylase [Bradyrhizobium japonicum USDA 110] dbj|BAC52868.1| mdlC [Bradyrhizobium japonicum USDA 110] Length = 553 Score = 606 bits (1563), Expect = e-171, Method: Compositional matrix adjust. Identities = 296/364 (81%), Positives = 329/364 (90%), Gaps = 5/364 (1%) Query: 1 MKNRITGRSAFLALLKDEGVTHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMAD 60 MKN+ITGRSAFLALLKDEG+THLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVA+AD Sbjct: 1 MKNKITGRSAFLALLKDEGITHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAIAD 60 Query: 61 GFSRASGKLVACNVHVAPGLGNAMGSLYNASFTGTPLILTAGQQEQGHGLTEPVLYGPLV 120 G+SRASGKLVACNVHVAPGLGNAMGSLYNA FTGTP+ILTAGQQEQGHGL EPVLYGPLV Sbjct: 61 GYSRASGKLVACNVHVAPGLGNAMGSLYNAQFTGTPMILTAGQQEQGHGLMEPVLYGPLV 120 Query: 121 QMATPLVKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFISLPGDILNAEAGIELGRSSR 180 +MA PLVKWAVEVTRLEDLPRIVRRAAK+A TPPTGPVFISLPGDILN+EAGI+LGRS+R Sbjct: 121 RMAEPLVKWAVEVTRLEDLPRIVRRAAKVAMTPPTGPVFISLPGDILNSEAGIDLGRSTR 180 Query: 181 IDTRVRPSEDSLKALAARILKAERPVIVVGDEIVKSDALREAADLAETLGCPAWQSPTPY 240 ID R +PS+++LKA AAR+LKAERPVIV DE+VKSDAL+EAA+LAE LG A+QS TPY Sbjct: 181 IDARTKPSDEALKAFAARLLKAERPVIVTMDEVVKSDALKEAAELAELLGAAAYQSSTPY 240 Query: 241 GSHFLSESPSFMGAIARLQKVARDVLAPHDLLIALGGDPLRMSVYSEVDPLPDGLSIVQV 300 GSHFLSESPSF+G +AR+QKVARD LAP+DLLIALGGDPLRMSVYSEVDPLPDGL I+QV Sbjct: 241 GSHFLSESPSFVGTLARVQKVARDTLAPYDLLIALGGDPLRMSVYSEVDPLPDGLPILQV 300 Query: 301 GLVDSDLARNYGAEIALKADVKETLRALVPALKEAGGGRRAPGRGSRRCRQETGRRSGRR 360 GLVD ++A+N+GAEIALKADVKETLRAL+P LKE GG A +R RQ + + Sbjct: 301 GLVDWEIAKNFGAEIALKADVKETLRALIPVLKEMGGAALA-----QRARQRLAELAPKN 355 Query: 361 WSSR 364 WS+R Sbjct: 356 WSAR 359 >ref|YP_001237761.1| hypothetical protein BBta_1644 [Bradyrhizobium sp. BTAi1] gb|ABQ33855.1| Putative Thiamine pyrophosphate-requiring enzyme [Bradyrhizobium sp. BTAi1] Length = 554 Score = 573 bits (1477), Expect = e-161, Method: Compositional matrix adjust. Identities = 279/369 (75%), Positives = 320/369 (86%), Gaps = 5/369 (1%) Query: 1 MKNRITGRSAFLALLKDEGVTHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMAD 60 MKNR TGRSAFLALLKDEG+THLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMAD Sbjct: 1 MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMAD 60 Query: 61 GFSRASGKLVACNVHVAPGLGNAMGSLYNASFTGTPLILTAGQQEQGHGLTEPVLYGPLV 120 GFSRASG+LVACNVHVAPGLGNAMGSLYNA FTGTP+ILTAGQQEQGHGL EP+LYGPLV Sbjct: 61 GFSRASGRLVACNVHVAPGLGNAMGSLYNAQFTGTPMILTAGQQEQGHGLMEPLLYGPLV 120 Query: 121 QMATPLVKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFISLPGDILNAEAGIELGRSSR 180 +MA PLVKWAVEVTRLEDLPRIVRRAAK+ATTPPTGPVFISLPGDILNAEAGI+LGRS+R Sbjct: 121 RMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFISLPGDILNAEAGIDLGRSTR 180 Query: 181 IDTRVRPSEDSLKALAARILKAERPVIVVGDEIVKSDALREAADLAETLGCPAWQSPTPY 240 +D RVRPS D+L+ALA RIL+A+RPVI+ GDEIVKSDAL+ AAD A LG P Q PY Sbjct: 181 VDARVRPSADALQALAQRILRAQRPVIITGDEIVKSDALQAAADFASALGAPVHQQSAPY 240 Query: 241 GSHFLSESPSFMGAIARLQKVARDVLAPHDLLIALGGDPLRMSVYSEVDPLPDGLSIVQV 300 G+HFLSE P ++G+++R+QK RD+LAPHDL+I LG DPLRMSV+SE++PLPDGL+IVQ+ Sbjct: 241 GAHFLSEHPCYIGSLSRIQKQVRDLLAPHDLMIVLGADPLRMSVHSEIEPLPDGLAIVQI 300 Query: 301 GLVDSDLARNYGAEIALKADVKETLRALVPALKEAGGGRRAPGRGSRRCRQETGRRSGRR 360 GLVD +L +N+GAEIA+KADV+ETL L P L+E GG A + + + R T + + Sbjct: 301 GLVDWELGKNFGAEIAIKADVRETLLQLTPLLRERGG---AALQATAQAR--TAELASKN 355 Query: 361 WSSRSRRRA 369 WS+R R A Sbjct: 356 WSARRRTLA 364 >ref|YP_001208015.1| putative thiamine pyrophosphate-requiring enzyme benzoylformate decarboxylase [Bradyrhizobium sp. ORS 278] emb|CAL79800.1| Putative Thiamine pyrophosphate-requiring enzyme; putative Benzoylformate decarboxylase [Bradyrhizobium sp. ORS 278] Length = 554 Score = 538 bits (1385), Expect = e-151, Method: Compositional matrix adjust. Identities = 273/366 (74%), Positives = 308/366 (84%), Gaps = 5/366 (1%) Query: 1 MKNRITGRSAFLALLKDEGVTHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMAD 60 MKNR TGRSAFLALLKDEG+THLFGNPGTTELP+MHALKDHPDLTYVMAMQESLVVA+AD Sbjct: 1 MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIAD 60 Query: 61 GFSRASGKLVACNVHVAPGLGNAMGSLYNASFTGTPLILTAGQQEQGHGLTEPVLYGPLV 120 GFSRASG+LVACNVHVAPGLGNAMGSLYNA FTGTP+ILTAGQQEQ HGL EP+LYGPLV Sbjct: 61 GFSRASGRLVACNVHVAPGLGNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLV 120 Query: 121 QMATPLVKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFISLPGDILNAEAGIELGRSSR 180 +MA PLVKWAVEVTRLEDLPRIVRRAAK+ATTPPTGPVFISLPGDILNAEAGIELGR++R Sbjct: 121 RMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFISLPGDILNAEAGIELGRATR 180 Query: 181 IDTRVRPSEDSLKALAARILKAERPVIVVGDEIVKSDALREAADLAETLGCPAWQSPTPY 240 +D RV PS +L+ALA RILKA+RPVI+ GDEIVKSDALR AAD A +G P Q PY Sbjct: 181 VDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY 240 Query: 241 GSHFLSESPSFMGAIARLQKVARDVLAPHDLLIALGGDPLRMSVYSEVDPLPDGLSIVQV 300 G+HFLSE P ++G+++R QK RD+LAPHDLLI LG DPLRMSV+SE +PLP+GL IVQ+ Sbjct: 241 GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQI 300 Query: 301 GLVDSDLARNYGAEIALKADVKETLRALVPALKEAGGGRRAPGRGSRRCRQETGRRSGRR 360 GLVD +L +N+GAEIALKADV+ETL L P L+ GG +R S + Sbjct: 301 GLVDWELGKNFGAEIALKADVRETLLQLTPLLRALGGSALQAAAQAR-----IAELSPKN 355 Query: 361 WSSRSR 366 WS+R R Sbjct: 356 WSARRR 361 >ref|ZP_00052667.2| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] [Magnetospirillum magnetotacticum MS-1] Length = 562 Score = 246 bits (627), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 142/349 (40%), Positives = 206/349 (59%), Gaps = 9/349 (2%) Query: 7 GRSAFLALLKDEGVTHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMADGFSRAS 66 G + +L+ EGV ++FGNPGTTELP++ AL D D+ YV+A+QE+ VAMADG+++A+ Sbjct: 14 GADVLVEVLRSEGVRYVFGNPGTTELPLIDALTDASDIAYVLALQEASAVAMADGYAQAA 73 Query: 67 GKLVACNVHVAPGLGNAMGSLYNASFTGTPLILTAGQQEQGHGLTEPVLYGPLVQMATPL 126 + N+H A GLG+ MG+L NA +GTPL++TAGQQ+ H +T+P+L+G LV +A+P Sbjct: 74 RRPGFLNLHTAGGLGHGMGNLLNAQISGTPLVVTAGQQDSRHVITDPLLFGDLVTIASPT 133 Query: 127 VKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFISLPGDILNAEAGIELGRSSRIDTRVR 186 VKWA EVT + LP ++RRA +T P+GPVF+SLP D++ A LG S ID R Sbjct: 134 VKWAREVTHADQLPVLLRRAFHDSTAAPSGPVFLSLPMDVMEARTTAPLGEISTIDH--R 191 Query: 187 PSEDSLKALAARI--LKAERPVIVVGDEIVKSDALREAADLAETLGCPAWQSPTPYGSHF 244 P SL LA R+ + R ++ GDE+ SDA +A LA+ L P + S P F Sbjct: 192 PVAGSLDRLADRLAAIPPGRLALIAGDEVDMSDAAAQAVRLADLLAAPVYGSSWPAHIPF 251 Query: 245 LSESPSFMGAIARLQKVARDVLAPHDLLIALGGDPLRMSVYSEVDPLPDGLSIVQVGLVD 304 S P +MG + ++L +D + ALGG L +Y+E +P G + Q+ Sbjct: 252 PSAHPLWMGNLPTKASGIAEILGRYDAVFALGGKSLITVLYTEGSAVPPGTQVFQLSADA 311 Query: 305 SDLARNYGAEIALKADVKETLRALVPALKEAGGGRRAP-GRGSRRCRQE 352 DL R Y +++ DV+ +L L+P L++ R AP R R E Sbjct: 312 RDLGRTYATQLSTVGDVRASLDVLIPLLED----RVAPRAEAYARLRSE 356 >ref|YP_003068764.1| decarboxylase, Thiamine pyrophosphate-requiring [Methylobacterium extorquens DM4] emb|CAX24908.1| Putative decarboxylase, Thiamine pyrophosphate-requiring [Methylobacterium extorquens DM4] Length = 562 Score = 237 bits (604), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 133/328 (40%), Positives = 194/328 (59%), Gaps = 2/328 (0%) Query: 7 GRSAFLALLKDEGVTHLFGNPGTTELPIMHALKDHPDLTYVMAMQESLVVAMADGFSRAS 66 G L +L+ EGV ++FGNPGTTELP++ AL D PD+ YV+A+QE+ VAMADG+++ + Sbjct: 14 GADVLLEVLRSEGVRYIFGNPGTTELPLIDALTDAPDIAYVLALQEASAVAMADGYAQGA 73 Query: 67 GKLVACNVHVAPGLGNAMGSLYNASFTGTPLILTAGQQEQGHGLTEPVLYGPLVQMATPL 126 + N+H A GLG+ MG+L NA +GTPL++TAGQQ+ H +T+P+L+G L +A P Sbjct: 74 RRPGFLNLHTAGGLGHGMGNLLNAQISGTPLVVTAGQQDSRHVITDPLLFGDLTTIAAPT 133 Query: 127 VKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFISLPGDILNAEAGIELGRSSRIDTR-V 185 VKWA EVT + LP ++RRA + P+GPVF+SLP D++ A LG S ID R V Sbjct: 134 VKWAREVTHADQLPVLLRRAFHDSAAAPSGPVFLSLPMDVMEAMTAAPLGEISTIDHRAV 193 Query: 186 RPSEDSLKALAARILKAERPVIVVGDEIVKSDALREAADLAETLGCPAWQSPTPYGSHFL 245 S D L A I R ++ GDE+ SDA +A LA+ L P + S P F Sbjct: 194 AGSLDRLADHLAGIAPG-RLALIAGDEVDASDASTQAVRLADLLAAPVYGSSWPAHIPFP 252 Query: 246 SESPSFMGAIARLQKVARDVLAPHDLLIALGGDPLRMSVYSEVDPLPDGLSIVQVGLVDS 305 + P +MG + ++L +D + ALGG L +Y+E +P G + Q+ Sbjct: 253 TAHPLWMGNLPTKASGIAEILGRYDAVFALGGKSLITVLYTEGSAVPPGTQVFQLSADAR 312 Query: 306 DLARNYGAEIALKADVKETLRALVPALK 333 DL R Y +++ +V+ +L L+P L+ Sbjct: 313 DLGRTYATQLSTVGNVRASLDVLLPLLE 340