BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0116.0015.p (325 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08629441.1| alcohol dehydrogenase [Bradyrhizobiaceae bact... 285 6e-75 ref|YP_629107.1| zinc-binding dehydrogenase family oxidoreductas... 239 4e-61 ref|YP_004381269.1| alcohol dehydrogenase [Pseudomonas mendocina... 234 2e-59 ref|YP_001188676.1| alcohol dehydrogenase [Pseudomonas mendocina... 229 5e-58 ref|YP_886510.1| trans-2-enoyl-CoA reductase [Mycobacterium smeg... 228 7e-58 >ref|ZP_08629441.1| alcohol dehydrogenase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07934.1| alcohol dehydrogenase [Bradyrhizobiaceae bacterium SG-6C] Length = 325 Score = 285 bits (730), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 152/322 (47%), Positives = 205/322 (63%) Query: 1 MKAVQVVAFGRAADVVKLNEVPDVGSPGPDEVVVAVEAAPINNSDFMIIAGRYGYLPSPP 60 M +VQ+ AFG+ DVV+ PD G+PG EVV+ +E +PIN SD +++ G YG P P Sbjct: 1 MHSVQINAFGKPWDVVETVATPDPGAPGAGEVVIDMEFSPINPSDLVLMRGLYGVKPKLP 60 Query: 61 ATLGIEGVGRVVATGSQVRNLKEGDRTLIPFTVPSWTERVKFTASWQRALPDNADVQQLS 120 A +G EGV RV GS V LKEGDR L P +W R K A ALP +AD QQLS Sbjct: 61 APVGSEGVARVTKAGSGVTGLKEGDRVLFPRGASTWVARSKVKADGLFALPASADPQQLS 120 Query: 121 MIGVNPATAYLLLTDFVKVPRGGWVIQNGANSATARAVIAIAKSLGLKTVNIVRREDVVD 180 M+ VNP TAYL+LT++V + +G WVIQ+ NSA RAVIAIA+ LG+ TV++VRR ++ Sbjct: 121 MLMVNPPTAYLMLTEYVALKKGDWVIQSAGNSAVGRAVIAIARKLGIHTVSVVRRPALIG 180 Query: 181 EVKSVGGDIVLLDGPDLAKRVARETGRAPIQLALDMVGGSSALNLMNCLAPKGVLVIYSA 240 E+K++G D+VL++GPDLAKRVA TG+A I LALD VGG + L +CLA G LV Y Sbjct: 181 ELKNLGADVVLVEGPDLAKRVAEATGKAKIMLALDSVGGPGLMALNDCLANGGTLVAYGV 240 Query: 241 MSGQPFSGSALSVIFNEVSVRGFWLGHWGKTATDQALAGMYDHLVPMVASGAISAPVVGA 300 MSG P IF +++++GFWL +W + + +A + + +A G I PV Sbjct: 241 MSGGPGPFFTAPNIFRDLTLKGFWLLNWFNRNSPERIAEVQKQMAAWIADGTIFTPVEAT 300 Query: 301 YRLEDFSQAIAQAAAFKGKVIF 322 Y L+ + AI+ AA GK++F Sbjct: 301 YSLDQSAAAISHAAKGTGKILF 322 >ref|YP_629107.1| zinc-binding dehydrogenase family oxidoreductase [Myxococcus xanthus DK 1622] gb|ABF92060.1| oxidoreductase, zinc-binding dehydrogenase family [Myxococcus xanthus DK 1622] Length = 328 Score = 239 bits (611), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 139/327 (42%), Positives = 192/327 (58%), Gaps = 4/327 (1%) Query: 1 MKAVQVVAFGRAADVVKLNEVPDVGSPGPDEVVVAVEAAPINNSDFMIIAGRYGYLPSPP 60 MKAV+ AFG+ VV++ E PDV + P E V V A PIN SD + ++G+YG LP P Sbjct: 1 MKAVRFSAFGQPLKVVEVVEQPDV-ALKPGEARVEVLATPINPSDILTLSGQYGQLPKLP 59 Query: 61 ATLGIEGVGRVVATGSQVRNLKEGDRTLIPFTVPSWTERVKFTASWQRALPDNADVQQLS 120 A G EGVGRVV +K GD +P +W + A +P D++Q + Sbjct: 60 AVPGNEGVGRVVEV-QDSSAVKVGDIVFLPLGAGTWCTHLVAPADSLLRVPPGTDLRQAA 118 Query: 121 MIGVNPATAYLLLTDFVKVPRGGWVIQNGANSATARAVIAIAKSLGLKTVNIVRREDVVD 180 M+ +NP TA LLL DF+ + G WVIQN ANSA R++I +AK G KT+N+VRRE++ Sbjct: 119 MLFINPPTADLLLRDFIALQPGEWVIQNAANSAVGRSIITLAKQAGFKTLNVVRREELAP 178 Query: 181 EVKSVGGDIVLLDGPDLAKRVARETGRAPIQLALDMVGGSSALNLMNCLAPKGVLVIYSA 240 E+ ++G D VLLD +L +RV TG A ++LA+D VGG S L + LA GV+V Y A Sbjct: 179 ELTALGADAVLLDSDELPERVREVTGGAKVRLAIDAVGGESTQRLGDALARGGVVVNYGA 238 Query: 241 MSGQPFSGSALSVIFNEVSVRGFWLGHWGKTATDQALAGMYDHLVPMVASGAISAPVVGA 300 MSG+ SA + IF ++++RGFWL W K + ++ L VA GA+ PV G Sbjct: 239 MSGKGPRLSAAATIFKDITLRGFWLVTWTKKTPREEQGALFARLAKQVAEGALHVPVEGT 298 Query: 301 YRLEDFSQAIAQA--AAFKGKVIFTPN 325 + LE +A+ +A GKV+ TPN Sbjct: 299 FPLESIQEALTRAMEGGRAGKVLLTPN 325 >ref|YP_004381269.1| alcohol dehydrogenase [Pseudomonas mendocina NK-01] gb|AEB59517.1| alcohol dehydrogenase [Pseudomonas mendocina NK-01] Length = 325 Score = 234 bits (597), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 137/301 (45%), Positives = 176/301 (58%), Gaps = 3/301 (0%) Query: 26 SPGPDEVVVAVEAAPINNSDFMIIAGRYGYLPSPPATLGIEGVGRVVATGSQVRNLKEGD 85 P +V V V AAPIN SD + + G YG LP PA G EGVG+V A G V N K G Sbjct: 26 EPAAGQVRVKVLAAPINPSDVLTLTGAYGMLPPLPAVGGNEGVGKVEALGEGVSNFKVGQ 85 Query: 86 RTLIPFTVPSWTERVKFTASWQRALPDNADVQQLSMIGVNPATAYLLLTDFVKVPRGGWV 145 L+P +W + A LPD AD QQL+M+ VNP TA LLL++FV + G WV Sbjct: 86 NVLLPVGCGTWVSAMNAPADKLIPLPD-ADPQQLAMLTVNPPTASLLLSEFVDLKPGDWV 144 Query: 146 IQNGANSATARAVIAIAKSLGLKTVNIVRREDVVDEVKSVGGDIVLLDGPDLAKRVARET 205 IQN ANS +I +AK G KT+N+VRRE V V++ GGD+VL+DGPDLAKRV T Sbjct: 145 IQNAANSGVGSYLIQLAKLRGFKTINVVRRESAVAAVEAEGGDLVLVDGPDLAKRVRAAT 204 Query: 206 GRAPIQLALDMVGGSSALNLMNCLAPKGVLVIYSAMSGQPFSGSALSVIFNEVSVRGFWL 265 G A ++L +D VGG S NL LA GVLV Y MSGQ S S +F +V++RGFWL Sbjct: 205 GGADVRLGIDAVGGVSTDNLAAVLANGGVLVNYGMMSGQACQVSPASFVFRDVTLRGFWL 264 Query: 266 GHWGKTATDQALAGMYDHLVPMVASGAISAPVVGAYRLEDFSQAIAQAAAFK--GKVIFT 323 W + A+ ++ LV ++ASG + V Y L A+A AA+ + GK++ Sbjct: 265 AKWFQQASPAQQMKVFGELVQLIASGKLKTRVAATYDLAHIKDAVAAAASGERDGKILLV 324 Query: 324 P 324 P Sbjct: 325 P 325 >ref|YP_001188676.1| alcohol dehydrogenase [Pseudomonas mendocina ymp] gb|ABP85944.1| Alcohol dehydrogenase GroES domain protein [Pseudomonas mendocina ymp] Length = 325 Score = 229 bits (584), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 134/301 (44%), Positives = 176/301 (58%), Gaps = 3/301 (0%) Query: 26 SPGPDEVVVAVEAAPINNSDFMIIAGRYGYLPSPPATLGIEGVGRVVATGSQVRNLKEGD 85 P +V V V AAPIN SD + + G YG LP PA G EGVGRV G V N K G Sbjct: 26 EPAAGQVRVKVLAAPINPSDVLTLTGAYGMLPPLPAIGGNEGVGRVEVLGEGVGNFKVGQ 85 Query: 86 RTLIPFTVPSWTERVKFTASWQRALPDNADVQQLSMIGVNPATAYLLLTDFVKVPRGGWV 145 L+P +W + A LPD AD QL+M+ VNP TA LLL++FV + G WV Sbjct: 86 TVLLPVGCGTWVTAMNAPADKLIPLPD-ADPLQLAMLTVNPPTASLLLSEFVDLKPGDWV 144 Query: 146 IQNGANSATARAVIAIAKSLGLKTVNIVRREDVVDEVKSVGGDIVLLDGPDLAKRVARET 205 IQN ANS +I +AK G KT+N+VRRE V V++ GGD+VL+DGPDLAKRV T Sbjct: 145 IQNAANSGVGSYLIQLAKLRGFKTINVVRRESAVAGVEAEGGDLVLVDGPDLAKRVRAAT 204 Query: 206 GRAPIQLALDMVGGSSALNLMNCLAPKGVLVIYSAMSGQPFSGSALSVIFNEVSVRGFWL 265 G A ++L +D VGG+S ++ LA GVLV Y MSG+ S S +F +V++RGFWL Sbjct: 205 GGAEVRLGIDAVGGASTDHIAATLANGGVLVNYGMMSGEACQVSPASFVFRDVTLRGFWL 264 Query: 266 GHWGKTATDQALAGMYDHLVPMVASGAISAPVVGAYRLEDFSQAIAQAAAFK--GKVIFT 323 W + A+ ++ LV ++ASG + V Y LE A+A AA+ + GK++ Sbjct: 265 AKWFQQASPAQQMKVFGELVQLIASGKLKTRVAATYDLEHIKDAVAAAASGERDGKILLV 324 Query: 324 P 324 P Sbjct: 325 P 325 >ref|YP_886510.1| trans-2-enoyl-CoA reductase [Mycobacterium smegmatis str. MC2 155] gb|ABK76225.1| trans-2-enoyl-CoA reductase [Mycobacterium smegmatis str. MC2 155] Length = 327 Score = 228 bits (582), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 130/327 (39%), Positives = 196/327 (59%), Gaps = 6/327 (1%) Query: 1 MKAVQVVAFGRAADVVKLNEVPDVGSPGPDEVVVAVEAAPINNSDFMIIAGRYGYLPSPP 60 MK + + FG D V+L + P+ + GP +V+V +EAA IN SD +++ G+Y P P Sbjct: 1 MKQLILTKFGDPEDTVRLVDTPEPVA-GPGKVLVRLEAAAINPSDLLLLKGKYLVHPELP 59 Query: 61 ATLGIEGVGRVVATGSQVRNLKEGDRTLI--PFTVPSWTERVKFTASWQRALPDNADVQQ 118 A +G EGVG V A G +V + G R ++ +T +W+E+V A +PD AD QQ Sbjct: 60 AGVGAEGVGIVEAVGPEVDSNLVGKRVIVLPTYTYGTWSEKVVAAAHDVIEVPD-ADPQQ 118 Query: 119 LSMIGVNPATAYLLLTDFVKVPRGGWVIQNGANSATARAVIAIAKSLGLKTVNIVRREDV 178 L+M+ +NP TA+LLL FV + G WV Q ANSA R V +A+ GLKT+NIVRRED Sbjct: 119 LAMLSINPPTAHLLLDRFVNLQVGDWVGQTAANSAVGRYVTTLARRRGLKTLNIVRREDA 178 Query: 179 VDEVKSVGGDIVLLDGPDLAKRVARETGRAPIQLALDMVGGSSALNLMNCLAPKGVLVIY 238 V++V++ GGD+V++ GP+LA + + G + L +D +GGS+A +L+ L G V Y Sbjct: 179 VEDVRAAGGDVVVVSGPNLADDIKQALGGDRLSLVIDPLGGSAASDLIGALEFGGTAVAY 238 Query: 239 SAMSGQPFSGSALSVIFNEVSVRGFWLGHWGKTATDQALAGMYDHLVPMVASGAISAPVV 298 ++++G P S S+ + EV + GFWLG+W + + D+L ++A G + APV Sbjct: 239 ASLTGTPMSVSSADLFGREVRLTGFWLGNWYASEPRHEIVDTLDYLARLIAEGVLHAPVE 298 Query: 299 GAYRLEDFSQAIAQAAAFK--GKVIFT 323 Y L+D+ +A A K GK++FT Sbjct: 299 ATYHLDDYLKAFTHARTAKRGGKILFT 325