BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0109.0011.p (475 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_772932.1| hemolysin secretion protein D [Bradyrhizobium j... 863 0.0 ref|ZP_07027188.1| type I secretion membrane fusion protein, Hly... 542 e-152 ref|YP_001208063.1| HlyD family secretion protein [Bradyrhizobiu... 436 e-120 ref|YP_003799067.1| putative type I secretion membrane fusion pr... 374 e-101 ref|YP_865881.1| HlyD family type I secretion membrane fusion pr... 357 2e-96 >ref|NP_772932.1| hemolysin secretion protein D [Bradyrhizobium japonicum USDA 110] dbj|BAC51557.1| hlyD [Bradyrhizobium japonicum USDA 110] Length = 513 Score = 863 bits (2229), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/475 (89%), Positives = 452/475 (95%), Gaps = 1/475 (0%) Query: 1 MTSASRNVVAFPRREPRRREHEIAFLPAALEIAESPPSPIGRAIGACIIAAFCIALLWAT 60 MTSA+RN+V FPR E RRREHEIAFLPAALEI ESPPSPIGRAIG IIA FC+ALLWA+ Sbjct: 40 MTSANRNIVPFPRAEVRRREHEIAFLPAALEITESPPSPIGRAIGGSIIAVFCVALLWAS 99 Query: 61 LGSVDIVASATGKIVPGGRTKLIQPFETGVVRAIRVRDGQSVKAGDVLIELDPTMTEADQ 120 LGSVDIVA+ATGKIVPGGRTKLIQPFETGVVRAI VRDGQSVKAGDVLIELDPTMTEADQ Sbjct: 100 LGSVDIVATATGKIVPGGRTKLIQPFETGVVRAIHVRDGQSVKAGDVLIELDPTMTEADQ 159 Query: 121 ERQKSDLLAAELDVARLRAALAADPLAAFRPPQNASANEIEMHRQFLISQRAEQSAKLSE 180 ERQ+SDL+AAELDVARLRAAL +DPLAAFR PQ AS E+EMHRQFLISQRAEQSAKL+E Sbjct: 160 ERQRSDLVAAELDVARLRAALTSDPLAAFRSPQGASVAEVEMHRQFLISQRAEQSAKLAE 219 Query: 181 IERQQGQKEAERATTSASVDKLEATIPVLKERVDIRKGLVEKALASKVVYLSEYQELVAM 240 IERQQGQKEAERATTSASV KL+ATIPVL+ERVDIRKGLV+KALASKV+YLSEYQELV + Sbjct: 220 IERQQGQKEAERATTSASVAKLQATIPVLQERVDIRKGLVDKALASKVIYLSEYQELVGL 279 Query: 241 QQDLVLQQSRLREADAAIALLKETKDKTAAEYRRATYDALAKAEQKAASAAQEVVKADRR 300 QQDLVLQQSRL EADAAIALLKETK++TAAEYRRATYDALAKAEQKAASAAQEVVKA+RR Sbjct: 280 QQDLVLQQSRLGEADAAIALLKETKERTAAEYRRATYDALAKAEQKAASAAQEVVKAERR 339 Query: 301 TKLQSLTAPVDGVVQQLAVHTVGGVVTPAQALAVVVPSESQLEIEAMLSNRDIGFVHPGQ 360 TKLQ LTAPVDGVVQQLAVHTVGGVVTPAQALA+VVPSESQLEIEAMLSNRDIGFVHPGQ Sbjct: 340 TKLQHLTAPVDGVVQQLAVHTVGGVVTPAQALAIVVPSESQLEIEAMLSNRDIGFVHPGQ 399 Query: 361 KAEIKVDTFNFTRYGLLHGDVLSVSTDAITRDKQQGGPNDRTLGAAQSSSEPKGQELEYA 420 KAEIKVDTFNFTRYGLL+GDVLSVSTDAITRD+ QGGPNDR G AQ SSEPKGQELEYA Sbjct: 400 KAEIKVDTFNFTRYGLLNGDVLSVSTDAITRDR-QGGPNDRASGTAQGSSEPKGQELEYA 458 Query: 421 ARVSLDRTHMQIEDKLVKLGPGMAVTVEIKTGTRRIISYLLSPLARYRQEALRER 475 AR+SLDRTHMQ+EDKLVKLGPGMAVTVEIKTG RRIISYLLSPLARY+QE+LRER Sbjct: 459 ARISLDRTHMQVEDKLVKLGPGMAVTVEIKTGARRIISYLLSPLARYKQESLRER 513 >ref|ZP_07027188.1| type I secretion membrane fusion protein, HlyD family [Afipia sp. 1NLS2] gb|EFI50944.1| type I secretion membrane fusion protein, HlyD family [Afipia sp. 1NLS2] Length = 431 Score = 542 bits (1397), Expect = e-152, Method: Compositional matrix adjust. Identities = 273/434 (62%), Positives = 338/434 (77%), Gaps = 6/434 (1%) Query: 44 IGACIIAAFCIALLWATLGSVDIVASATGKIVPGGRTKLIQPFETGVVRAIRVRDGQSVK 103 +GA IIA F +ALLWA+LG+VDIVA A GK++P GRTK+IQPFE GVVRAI + DG +VK Sbjct: 2 LGAAIIALFGVALLWASLGTVDIVAVAPGKVIPNGRTKIIQPFEAGVVRAIHIHDGSTVK 61 Query: 104 AGDVLIELDPTMTEADQERQKSDLLAAELDVARLRAALAA--DPLAAFRPPQNASANEIE 161 AGDVLIELDPT+ AD + + DL+AAELDVARLRAALA +P +F PPQ+A+ +E Sbjct: 62 AGDVLIELDPTINAADVKHMEIDLIAAELDVARLRAALANHDNPADSFTPPQDATKELVE 121 Query: 162 MHRQFLISQRAEQSAKLSEIERQQGQKEAERATTSASVDKLEATIPVLKERVDIRKGLVE 221 RQ+LISQ E AK++EI RQ QK AER T +A++ KLEATIP+L+ER +R+ L E Sbjct: 122 TQRQYLISQTQEHRAKIAEIGRQIAQKRAERDTIAANIAKLEATIPLLEERTKMREYLYE 181 Query: 222 KALASKVVYLSEYQELVAMQQDLVLQQSRLREADAAIALLKETKDKTAAEYRRATYDALA 281 L SK+ YLSE Q+LV Q + ++Q+SR +EADAAIA L ET+ + AEYRR D L+ Sbjct: 182 NKLGSKITYLSELQDLVGQQHETLVQKSREQEADAAIAALDETQTRAKAEYRRTLLDDLS 241 Query: 282 KAEQKAASAAQEVVKADRRTKLQSLTAPVDGVVQQLAVHTVGGVVTPAQALAVVVPSESQ 341 KAEQKA Q+V+KA ++ KLQ LTAP+DGVVQQLAVHT+GGVVTPAQ++AV+V ++ Sbjct: 242 KAEQKAGGLRQDVIKAKQKAKLQILTAPIDGVVQQLAVHTIGGVVTPAQSIAVIVATDGG 301 Query: 342 LEIEAMLSNRDIGFVHPGQKAEIKVDTFNFTRYGLLHGDVLSVSTDAITRDKQQGGPNDR 401 +EIEAM+SNRDIGFV PGQ AEIKVDTFNFTRYGLLHG ++SVS DAIT +K + P Sbjct: 302 VEIEAMVSNRDIGFVFPGQTAEIKVDTFNFTRYGLLHGHIISVSRDAITPEKSREQP--- 358 Query: 402 TLGAAQSSSEPKGQELEYAARVSLDRTHMQIEDKLVKLGPGMAVTVEIKTGTRRIISYLL 461 + ++EPK QEL YAAR++LDRT MQI+DKL+ L PGMAVT EI+TG+RRIISYLL Sbjct: 359 -MQPESPATEPKNQELTYAARIALDRTQMQIDDKLIPLSPGMAVTAEIRTGSRRIISYLL 417 Query: 462 SPLARYRQEALRER 475 SPLARYR + LRER Sbjct: 418 SPLARYRHDILRER 431 >ref|YP_001208063.1| HlyD family secretion protein [Bradyrhizobium sp. ORS 278] emb|CAL79848.1| Putative HlyD family secretion protein [Bradyrhizobium sp. ORS 278] Length = 469 Score = 436 bits (1121), Expect = e-120, Method: Compositional matrix adjust. Identities = 245/470 (52%), Positives = 317/470 (67%), Gaps = 17/470 (3%) Query: 17 RRREHEIAFLPAALEIAESPPSPIGRAIGACIIAAFCIALLWATLGSVDIVASATGKIVP 76 RRR FLPAALEI E+P SP GRA+ I+ F IA+ W+ +G VDI+A+A GKIVP Sbjct: 6 RRRNDAREFLPAALEIVETPASPAGRAVAGTIMLFFAIAVGWSIIGHVDIIAAAQGKIVP 65 Query: 77 GGRTKLIQPFETGVVRAIRVRDGQSVKAGDVLIELDPTMTEADQERQKSDLLAAELDVAR 136 GRTK IQP E GVV AI V+DG V+AGDVL+ELD T+TEA++ R L+ A LDVAR Sbjct: 66 TGRTKTIQPLEAGVVAAIHVQDGDKVRAGDVLVELDRTVTEAERHRVAQGLMQARLDVAR 125 Query: 137 L---RAALAADPLAAFRP---PQNASANEIEMHRQFLISQRAEQSAKLSEIERQQGQKEA 190 L R + A LAA RP P+ A +I R L +Q AEQ +KL+ ++Q QK A Sbjct: 126 LSVLRDSFA--DLAALRPLSVPEGAPPTDIARTRAALQAQAAEQLSKLASNQQQIAQKRA 183 Query: 191 ERATTSASVDKLEATIPVLKERVDIRKGLVEKALASKVVYLSEYQELVAMQQDLVLQQSR 250 E + A++ K++A +P L+E DIR+ E +++ ++ L+ Q + V+Q R Sbjct: 184 EVQSIEAAIAKIDAALPFLRETADIRRNAKEIQYGNQIAFIDAQSRLIDQQSERVVQTRR 243 Query: 251 LREADAAIALLKETKDKTAAEYRRATYDALAKAEQKAASAAQEVVKADRRTKLQSLTAPV 310 L E +AA L++ +T + + R L AE+KA Q++VKA+R+T Q L AP+ Sbjct: 244 LVEIEAARMALEQQLAQTRSGFERQVLSDLTDAEKKADELTQDLVKAERKTSEQLLRAPI 303 Query: 311 DGVVQQLAVHTVGGVVTPAQALAVVVPSESQLEIEAMLSNRDIGFVHPGQKAEIKVDTFN 370 DG VQQLA+HTVGGVVTPAQ L ++VP++SQLE E M+SNRDIGFV+ GQ AEIK+DTFN Sbjct: 304 DGTVQQLALHTVGGVVTPAQQLMLIVPADSQLETEVMISNRDIGFVNVGQPAEIKIDTFN 363 Query: 371 FTRYGLLHGDVLSVSTDAITRDKQQGGPNDR-----TLGAAQSSSEPKGQELEYAARVSL 425 FTRYGL+HG VLSVS D+I R+K P D+ GA +SEP GQE Y+ARVSL Sbjct: 364 FTRYGLVHGKVLSVSQDSIVREK----PADKQAGGKAAGALAETSEPAGQEFIYSARVSL 419 Query: 426 DRTHMQIEDKLVKLGPGMAVTVEIKTGTRRIISYLLSPLARYRQEALRER 475 D MQ+EDK+V L PGMAVTVEIKTGTRRII Y++SPL RY+ E+LR+R Sbjct: 420 DEKQMQVEDKMVALSPGMAVTVEIKTGTRRIIEYIMSPLLRYKHESLRDR 469 >ref|YP_003799067.1| putative type I secretion membrane fusion protein HlyD [Candidatus Nitrospira defluvii] emb|CBK43142.1| putative Type I secretion membrane fusion protein HlyD [Candidatus Nitrospira defluvii] Length = 463 Score = 374 bits (959), Expect = e-101, Method: Compositional matrix adjust. Identities = 208/453 (45%), Positives = 285/453 (62%), Gaps = 21/453 (4%) Query: 23 IAFLPAALEIAESPPSPIGRAIGACIIAAFCIALLWATLGSVDIVASATGKIVPGGRTKL 82 + FLPA LEI +PPSPIGRAI I+ F A+ W++L +DIVA A GK +P G +K Sbjct: 32 VEFLPAVLEIQHAPPSPIGRAILWTIMLVFTTAVTWSSLSHIDIVAVAPGKTIPSGHSKT 91 Query: 83 IQPFETGVVRAIRVRDGQSVKAGDVLIELDPTMTEADQERQKSDLLAAELDVARLRAALA 142 IQPFETGV+ +I V+DGQ+V+ G+VLIELD T AD++R ++ AA ++ ARLRA +A Sbjct: 92 IQPFETGVIASIHVQDGQTVRQGEVLIELDATQNGADRDRALNEYRAALVEAARLRALIA 151 Query: 143 ADPLAAFRPPQNASANEIEMHRQFLISQRAEQSAKLSEIERQQGQKEAERATTSASVDKL 202 + F P+ A I + +Q L Q AE SA++ GQ+ A T ++ +L Sbjct: 152 G--YSTFEVPEEADPQFILLQQQLLRDQVAEFSARVDASRHLIGQRRAALDATKDNLRRL 209 Query: 203 EATIPVLKERVDIRKGLVEKALASKVVYLSEYQELVAMQQDLVLQQSRLREADAAIALLK 262 EAT+P+ ER + L+ + SK+ YL Q+ + Q+ Q+S+LR+ AA+A + Sbjct: 210 EATVPIEAERAKAYRSLLAQQYVSKMDYLQFEQQRIDKAQEWAGQKSKLRQDQAALAEAE 269 Query: 263 ETKDKTAAEYRRATYDALAKAEQKAASAAQEVVKADRRTKLQSLTAPVDGVVQQLAVHTV 322 + +E++++ L+ E K AS QEV KA ++ LQ L +P+DGVVQQLAVHTV Sbjct: 270 QNYHALLSEFQQSKQAELSTTETKTASLLQEVRKAGQKIHLQKLVSPIDGVVQQLAVHTV 329 Query: 323 GGVVTPAQALAVVVPSESQLEIEAMLSNRDIGFVHPGQKAEIKVDTFNFTRYGLLHGDVL 382 GGVVTPAQ L +VVP + +E+EA N+DIGFV GQ EIKV+TF FT YG + G VL Sbjct: 330 GGVVTPAQPLLIVVPQDHPVEVEAQFENKDIGFVKEGQPVEIKVETFPFTLYGTIPGKVL 389 Query: 383 SVSTDAITRDKQQGGPNDRTLGAAQSSSEPKGQELEYAARVSLDRTHMQIEDKLVKLGPG 442 SVS DA+ DK +GG L YA R+S+DR MQ+E + + L PG Sbjct: 390 SVSGDAVPLDKDKGG-------------------LIYAGRISMDRATMQVEGRHIHLSPG 430 Query: 443 MAVTVEIKTGTRRIISYLLSPLARYRQEALRER 475 MAV VE+KTG RR+I YLLSPL ++R+E+LRER Sbjct: 431 MAVIVEVKTGQRRVIEYLLSPLLKFRRESLRER 463 >ref|YP_865881.1| HlyD family type I secretion membrane fusion protein [Magnetococcus sp. MC-1] gb|ABK44475.1| type I secretion membrane fusion protein, HlyD family [Magnetococcus sp. MC-1] Length = 468 Score = 357 bits (917), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 195/460 (42%), Positives = 280/460 (60%), Gaps = 25/460 (5%) Query: 20 EHEIAFLPAALEIAESPPSPIGRAIGACIIAAFCIALLWATLGSVDIVASATGKIVPGGR 79 +HE AF PA LE+ E PPSP GR I I+ +A+ W+ G +D+VA A GKI+P G Sbjct: 30 DHENAFKPAVLEVLERPPSPFGRLITWTIMLFCALAVGWSIWGQIDVVAVAQGKILPNGH 89 Query: 80 TKLIQPFETGVVRAIRVRDGQSVKAGDVLIELDPTMTEADQERQKSDLLAAELDVARLRA 139 K+IQP E GVVR I VR+G+ V G+VL+ LDPT TEAD R + +LL+A+LD+AR +A Sbjct: 90 VKVIQPLEIGVVREIPVREGEQVAEGEVLVILDPTSTEADLVRFEENLLSAQLDMARWQA 149 Query: 140 ALAAD----PLAAFRPPQNASANEIEMHRQFLISQRAEQSAKLSEIERQQGQKEAERATT 195 D P +R P A ++ R L A L I + ++ AE A+ Sbjct: 150 LAEWDQGEQPQILYRSPDGAPEGRVQKQRWLLKDTLLAHRASLQGIGHELKKQMAELASA 209 Query: 196 SASVDKLEATIPVLKERVDIRKGLVEKALASKVVYLSEYQELVAMQQDLVLQQSRLREAD 255 +V K + +P++ +R + +K L+EK LA++ +L QE V +Q+DL +Q+SRL E Sbjct: 210 KEAVRKYQQLLPLIAKRANAQKTLMEKKLAAQSHWLELEQERVELQRDLAIQKSRLAELL 269 Query: 256 AAIALLKETKDKTAAEYRRATYDALAKAEQKAASAAQEVVKADRRTKLQSLTAPVDGVVQ 315 A + + ++ +T A +R+ + ++EQ+ QE+ KA RR +LQ L +P+DGVVQ Sbjct: 270 AGLDVTRKRFAETEARFRKEVMEKYVESEQRVIDIEQELTKAARRNQLQVLRSPIDGVVQ 329 Query: 316 QLAVHTVGGVVTPAQALAVVVPSESQLEIEAMLSNRDIGFVHPGQKAEIKVDTFNFTRYG 375 L +HTVGGVV PAQ L +VP+ +E+EA L+N+DIGFV GQ EIK++TF FTRYG Sbjct: 330 NLVIHTVGGVVQPAQELMRIVPTSGGIEVEAYLANKDIGFVEEGQAVEIKLETFPFTRYG 389 Query: 376 LLHGDVLSVSTDAITRDKQQGGPNDRTLGAAQSSSEPKGQELEYAARVSLDRTHMQIEDK 435 L++G V +S DA+ ++ +G L YAAR+S+ + MQ+ ++ Sbjct: 390 LINGIVKKLSADAVEHEE---------MG------------LVYAARISMMKNTMQVGER 428 Query: 436 LVKLGPGMAVTVEIKTGTRRIISYLLSPLARYRQEALRER 475 LV + PGM+VT EIKTG RRII + LSP+ + QE LRER Sbjct: 429 LVNITPGMSVTAEIKTGKRRIIEFFLSPIQKSMQEGLRER 468