BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0104.0068.p (328 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08627674.1| 2-nitropropane dioxygenase-like protein [Brad... 586 e-165 ref|YP_001412104.1| 2-nitropropane dioxygenase NPD [Parvibaculum... 535 e-150 gb|AAR05212.1| predicted dioxygenase [uncultured marine proteoba... 472 e-131 gb|ACN58901.1| dioxygenases related to 2-nitropropane dioxygenas... 434 e-120 ref|ZP_01617976.1| hypothetical protein GP2143_01890 [marine gam... 422 e-116 >ref|ZP_08627674.1| 2-nitropropane dioxygenase-like protein [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09578.1| 2-nitropropane dioxygenase-like protein [Bradyrhizobiaceae bacterium SG-6C] Length = 324 Score = 586 bits (1511), Expect = e-165, Method: Compositional matrix adjust. Identities = 284/320 (88%), Positives = 298/320 (93%) Query: 6 MTLPASLANKLELPVVGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAEKLDEWLTQIE 65 M LPASLA KLELPV+GSPLFIVSGPELVIAQCKAG+VGSFPALNARPAEKL EWLTQIE Sbjct: 1 MPLPASLAGKLELPVLGSPLFIVSGPELVIAQCKAGVVGSFPALNARPAEKLGEWLTQIE 60 Query: 66 NELGEYQALHPEKKVAPYAVNQICHASNDRLMRDMETCVKHKVPVIITSLRPPSEIVEAA 125 +ELGEYQA +P+KK+APYAVNQICHASNDRLM+DMETCVKHKVPVIITSLRPPSEIV+AA Sbjct: 61 DELGEYQANNPDKKIAPYAVNQICHASNDRLMKDMETCVKHKVPVIITSLRPPSEIVDAA 120 Query: 126 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFNGTIL 185 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWF GTIL Sbjct: 121 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFKGTIL 180 Query: 186 LSGAISDGWSIASALALGADLAYIGTRFIATEEANADIRYKQALTEYAAHDIVYSNLFTG 245 LSGAISDGW IASALALGAD+AY+GTRFIAT EANAD YK+ALTEYAAHD VY+NLFTG Sbjct: 181 LSGAISDGWGIASALALGADMAYMGTRFIATVEANADEAYKKALTEYAAHDTVYTNLFTG 240 Query: 246 VHGNYLGPSIAAAGLDPGNLPVADKTKMNFGSGGNMKAKAWRDIWGSGQGIGQISDAPPV 305 VHGNYLGPSIAAAGLDP NLPVADKTKMNFGSGGNMKAKAWRDIWG GQGIGQI+D P V Sbjct: 241 VHGNYLGPSIAAAGLDPANLPVADKTKMNFGSGGNMKAKAWRDIWGCGQGIGQITDVPSV 300 Query: 306 GELVARLKSEFVAARSDFLR 325 ELV RLK EF A DF + Sbjct: 301 AELVERLKGEFNDATGDFAK 320 >ref|YP_001412104.1| 2-nitropropane dioxygenase NPD [Parvibaculum lavamentivorans DS-1] gb|ABS62447.1| 2-nitropropane dioxygenase NPD [Parvibaculum lavamentivorans DS-1] Length = 335 Score = 535 bits (1377), Expect = e-150, Method: Compositional matrix adjust. Identities = 251/318 (78%), Positives = 283/318 (88%) Query: 6 MTLPASLANKLELPVVGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAEKLDEWLTQIE 65 M LP L N LE+PV+GSPLFIVSGPELVIAQCKAGIVGSFPALNARPA L+EW+T+I+ Sbjct: 1 MALPDILKNSLEIPVIGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAHVLEEWITRIK 60 Query: 66 NELGEYQALHPEKKVAPYAVNQICHASNDRLMRDMETCVKHKVPVIITSLRPPSEIVEAA 125 ELGEYQA HPEKKVAP+AVNQICHASNDRL D++ C K +VP+IITSLRPP+E+++AA Sbjct: 61 GELGEYQAKHPEKKVAPFAVNQICHASNDRLAHDIDVCTKLEVPIIITSLRPPAEVIDAA 120 Query: 126 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFNGTIL 185 HSYGG VFHDVINVKHA+KAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWF GT++ Sbjct: 121 HSYGGKVFHDVINVKHAKKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFKGTVI 180 Query: 186 LSGAISDGWSIASALALGADLAYIGTRFIATEEANADIRYKQALTEYAAHDIVYSNLFTG 245 LSGA+SDGW IA ALA+GADLAY+GTRF+AT EANA+ YK L +YAA DIVY+NLFTG Sbjct: 181 LSGALSDGWGIAGALAMGADLAYMGTRFVATAEANAEQAYKDHLIQYAADDIVYTNLFTG 240 Query: 246 VHGNYLGPSIAAAGLDPGNLPVADKTKMNFGSGGNMKAKAWRDIWGSGQGIGQISDAPPV 305 VHGNYL PS+AAAGLDP NLP ADK+KMNFGSGGNMK KAW+DIWGSGQGIGQI+DAPPV Sbjct: 241 VHGNYLAPSVAAAGLDPNNLPEADKSKMNFGSGGNMKQKAWKDIWGSGQGIGQITDAPPV 300 Query: 306 GELVARLKSEFVAARSDF 323 EL+ RLK E+V A +F Sbjct: 301 AELIDRLKREYVQACREF 318 >gb|AAR05212.1| predicted dioxygenase [uncultured marine proteobacterium ANT8C10] gb|AAR05240.1| predicted dioxygenase [uncultured marine proteobacterium ANT32C12] Length = 321 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 227/318 (71%), Positives = 274/318 (86%) Query: 6 MTLPASLANKLELPVVGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAEKLDEWLTQIE 65 M +P+ + + L +PV+GSPLF+VSGPELVIAQCKAGI+GSFPALNARP L EW+++I+ Sbjct: 1 MPIPSHIKDHLSIPVIGSPLFLVSGPELVIAQCKAGIIGSFPALNARPQHVLGEWISRIK 60 Query: 66 NELGEYQALHPEKKVAPYAVNQICHASNDRLMRDMETCVKHKVPVIITSLRPPSEIVEAA 125 EL EYQ +P+ KVAP+AVNQICHASN+RLM DM TCV+H+VP+IITSLRPP+E+V+AA Sbjct: 61 TELAEYQEQNPDAKVAPFAVNQICHASNNRLMEDMATCVEHEVPIIITSLRPPAELVQAA 120 Query: 126 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFNGTIL 185 HSYGG+V+HDVINV+HA+KAAEQGVDGLILVCAGAGGHAG LSPFAL+REVK+WF+GT++ Sbjct: 121 HSYGGLVYHDVINVRHAKKAAEQGVDGLILVCAGAGGHAGALSPFALLREVKEWFDGTVI 180 Query: 186 LSGAISDGWSIASALALGADLAYIGTRFIATEEANADIRYKQALTEYAAHDIVYSNLFTG 245 LSG+I DG+S+ASALALGAD AY+GTRFIAT+EANAD YKQ L E AA DIV S+LFTG Sbjct: 181 LSGSIGDGYSVASALALGADFAYMGTRFIATQEANADQGYKQMLIESAADDIVLSSLFTG 240 Query: 246 VHGNYLGPSIAAAGLDPGNLPVADKTKMNFGSGGNMKAKAWRDIWGSGQGIGQISDAPPV 305 + GNYL PSI G+D NLP ADK+ MNFGSGGN AKAW+DIWGSGQGIG I D P V Sbjct: 241 ISGNYLKPSIVKEGMDADNLPEADKSSMNFGSGGNTDAKAWKDIWGSGQGIGGIKDDPSV 300 Query: 306 GELVARLKSEFVAARSDF 323 +LV RLK+EF+ A+++F Sbjct: 301 ADLVTRLKAEFIEAKNEF 318 >gb|ACN58901.1| dioxygenases related to 2-nitropropane dioxygenase [uncultured bacterium BLR18] Length = 325 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 210/325 (64%), Positives = 259/325 (79%), Gaps = 2/325 (0%) Query: 1 MEDPKMTLPASLANKLELPVVGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAEKLDEW 60 ME +P L N L +PV+ SP+FI SGP+LV AQCK GIVGSFPALNARPAE LD W Sbjct: 1 MERTMHRIPEVLRN-LSMPVIASPMFIASGPKLVAAQCKTGIVGSFPALNARPAELLDTW 59 Query: 61 LTQIENELGEYQALHPEKKVAPYAVNQICHASNDRLMRDMETCVKHKVPVIITSLR-PPS 119 LT+++ EL YQA +P+ + P AVNQI H SN RL D+E CV+HKVP++I+SLR PP Sbjct: 60 LTELQAELAAYQAANPDAIIGPIAVNQIVHHSNSRLEHDIEVCVRHKVPIVISSLRAPPP 119 Query: 120 EIVEAAHSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQW 179 ++++A HSYGG+V HDVIN++HA+KA E GVDGLILV +GAGGHAGTLSPFALV EV+++ Sbjct: 120 DMLDAIHSYGGIVLHDVINIRHAQKALEAGVDGLILVASGAGGHAGTLSPFALVGEVRRF 179 Query: 180 FNGTILLSGAISDGWSIASALALGADLAYIGTRFIATEEANADIRYKQALTEYAAHDIVY 239 FNG + LSG+I+ G +I +A A+GAD AYIG+R++ATEEAN D Y+QA+ E +A D+VY Sbjct: 180 FNGPVALSGSIATGDAILAAQAMGADFAYIGSRWLATEEANVDDAYRQAILESSAADVVY 239 Query: 240 SNLFTGVHGNYLGPSIAAAGLDPGNLPVADKTKMNFGSGGNMKAKAWRDIWGSGQGIGQI 299 +NLFTGVHGNYL SI AAGLDP NLPVADKTKM+FGSGG KAKAWRDIWG+GQG+G + Sbjct: 240 TNLFTGVHGNYLKKSIEAAGLDPENLPVADKTKMDFGSGGGNKAKAWRDIWGAGQGVGLM 299 Query: 300 SDAPPVGELVARLKSEFVAARSDFL 324 DAP V E+ ARLK+E+ AAR L Sbjct: 300 HDAPSVAEVAARLKAEYQAARERLL 324 >ref|ZP_01617976.1| hypothetical protein GP2143_01890 [marine gamma proteobacterium HTCC2143] gb|EAW30255.1| hypothetical protein GP2143_01890 [marine gamma proteobacterium HTCC2143] Length = 322 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 201/316 (63%), Positives = 246/316 (77%) Query: 6 MTLPASLANKLELPVVGSPLFIVSGPELVIAQCKAGIVGSFPALNARPAEKLDEWLTQIE 65 M LP L KL +PVV SPLFI+S P+LVIAQCKAG+VGSFP+LNARP E LDEWL +I Sbjct: 1 MALPDILKGKLSIPVVASPLFIISSPDLVIAQCKAGVVGSFPSLNARPIEMLDEWLHKIT 60 Query: 66 NELGEYQALHPEKKVAPYAVNQICHASNDRLMRDMETCVKHKVPVIITSLRPPSEIVEAA 125 EL EY A +P+ AP+AVNQI H SN+RL D+E C K+KVP++ITSL E+ E Sbjct: 61 TELAEYDAQNPDNPSAPFAVNQIVHKSNNRLNEDVELCKKYKVPIVITSLGASVELNEEI 120 Query: 126 HSYGGVVFHDVINVKHARKAAEQGVDGLILVCAGAGGHAGTLSPFALVREVKQWFNGTIL 185 HSYGG+V HD+IN ARKA +G DGLI V +GAGGHAG+ SPFAL+ E+++WF+G +L Sbjct: 121 HSYGGIVLHDIINDFFARKAIAKGADGLIAVASGAGGHAGSTSPFALISEIREWFDGPLL 180 Query: 186 LSGAISDGWSIASALALGADLAYIGTRFIATEEANADIRYKQALTEYAAHDIVYSNLFTG 245 LSG+I+ G ++ +A A+GADLAYIG+ FIATEEANA+ YKQAL +YA+ DIVY+NLFTG Sbjct: 181 LSGSIATGDAVLAAQAMGADLAYIGSAFIATEEANAEQGYKQALVDYASSDIVYTNLFTG 240 Query: 246 VHGNYLGPSIAAAGLDPGNLPVADKTKMNFGSGGNMKAKAWRDIWGSGQGIGQISDAPPV 305 VHGNYL PSI AAG+DP NLPV+D +KMNFGSGG+ KAW+DIWG GQGIG I D Sbjct: 241 VHGNYLKPSIIAAGMDPDNLPVSDPSKMNFGSGGSSDKKAWKDIWGCGQGIGAIKDVLSA 300 Query: 306 GELVARLKSEFVAARS 321 GELV RLKSE+ A++ Sbjct: 301 GELVTRLKSEYDTAKA 316