BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0104.0062.p (637 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08628755.1| chloride channel protein [Bradyrhizobiaceae b... 607 e-171 ref|ZP_07025062.1| Cl- channel voltage-gated family protein [Afi... 534 e-149 ref|YP_002288308.1| voltage gated chloride channel family protei... 524 e-146 ref|YP_004633599.1| chloride channel protein [Oligotropha carbox... 523 e-146 ref|YP_002421772.1| chloride channel core [Methylobacterium chlo... 386 e-105 >ref|ZP_08628755.1| chloride channel protein [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08560.1| chloride channel protein [Bradyrhizobiaceae bacterium SG-6C] Length = 586 Score = 607 bits (1566), Expect = e-171, Method: Compositional matrix adjust. Identities = 333/461 (72%), Positives = 385/461 (83%) Query: 1 MTTTSSYLEAPRRLRAFVRAHETSLVVLAVLIGAIGGLVVAVMSGLVTVLHSVLFNLPHG 60 M + +LEAP+RLRAFVRA E+SL+V+A LIGA+ GL VA MS V +LH VLF++P G Sbjct: 1 MAPSGYFLEAPQRLRAFVRARESSLIVIAALIGAMAGLFVAAMSAAVELLHVVLFDIPMG 60 Query: 61 DRLSSQPSIDPVRALVVPTIGGLLLGFAFLLLLRVRPAREIDPIEANALYGGRMSFRGSV 120 RLS+ +DP+RA+VVPT+GGL+LG A L+LLR RP REIDPIEANAL+GG MSFRGS+ Sbjct: 61 ARLSAINQVDPLRAIVVPTLGGLVLGIALLVLLRWRPGREIDPIEANALHGGYMSFRGSI 120 Query: 121 IVALQTIWSSGVGGSVGLEAGYTQLSSGLAASLGRGFHLRRNDQRIMVGCGAAAAIAGAF 180 IVALQTIWSSGVGGSVGLEAGYTQL+SG+A+S+GR FHLRR DQRI+VGCGA+AAIAGAF Sbjct: 121 IVALQTIWSSGVGGSVGLEAGYTQLASGVASSIGRAFHLRRADQRILVGCGASAAIAGAF 180 Query: 181 SAPLAGAFYAFELVIGGYTPASLTPVGVAAVTGYFVAHAFEPLPLGVGVGTVGDVLGRDL 240 SAPLAGAFYAFELV+GGYTPASL P+GVAAVTGY V H F PL LGV + +G+V +DL Sbjct: 181 SAPLAGAFYAFELVLGGYTPASLAPIGVAAVTGYGVTHLFAPLSLGVSIRQIGEVTSQDL 240 Query: 241 AIAALLGMVAALTGIAIMRGVTLCEALLTKSRLWPPLRPALGGLAVGAMALLTPQVMSSG 300 AIAA+LG++AALTGIAIMRGV L + L+K RLW PLRPALGGLAVG MAL+TPQV++SG Sbjct: 241 AIAAVLGLLAALTGIAIMRGVALFDTALSKIRLWTPLRPALGGLAVGLMALVTPQVLASG 300 Query: 301 HGALRFAGIVAMPLAFIAGVFALKAVASIVSLGSGFRGGLFFATLFMGALGGRLFAAGVD 360 HGAL ++ MP+ + VF LKA+ASIVSLGSGFRGGLFFA+L +G+LGG LFAAGV Sbjct: 301 HGALYLNSLIKMPVTLMLMVFLLKALASIVSLGSGFRGGLFFASLLLGSLGGHLFAAGVS 360 Query: 361 IVWPGLNLDPNAYAVIGMSALSASVIGGPLTMSFIALESTGNLWLTTAVLVAVIISTTIT 420 V PGLNLD YAVIGMSALSAS+IGGPLTMSFIALE+TGNLWLTTAVL+AVI+S +T Sbjct: 361 AVLPGLNLDHGVYAVIGMSALSASIIGGPLTMSFIALEATGNLWLTTAVLIAVIVSMQVT 420 Query: 421 RELFGYSFATWRLHLRGETIRSAADRRRRYRLDPRSHGRQD 461 RELFGYSFATWR HLRGETIRSAAD L R R D Sbjct: 421 RELFGYSFATWRFHLRGETIRSAADVGWIRDLTVRRMMRTD 461 >ref|ZP_07025062.1| Cl- channel voltage-gated family protein [Afipia sp. 1NLS2] gb|EFI52204.1| Cl- channel voltage-gated family protein [Afipia sp. 1NLS2] Length = 588 Score = 534 bits (1375), Expect = e-149, Method: Compositional matrix adjust. Identities = 295/443 (66%), Positives = 364/443 (82%) Query: 3 TTSSYLEAPRRLRAFVRAHETSLVVLAVLIGAIGGLVVAVMSGLVTVLHSVLFNLPHGDR 62 T+ YLEAPRRLRA VRA E+SL+VLA ++G +GGL V +MS V +LH VLFN+PHG+R Sbjct: 5 TSKLYLEAPRRLRALVRARESSLIVLAAIVGILGGLFVVLMSRAVGLLHEVLFNIPHGER 64 Query: 63 LSSQPSIDPVRALVVPTIGGLLLGFAFLLLLRVRPAREIDPIEANALYGGRMSFRGSVIV 122 LSSQ ++P+RA+++PT+GGLL G + L L R RP RE+DPIEANAL+GG+MSFRGS+IV Sbjct: 65 LSSQSVLEPMRAVIMPTLGGLLFGLSLLALHRWRPGREVDPIEANALHGGKMSFRGSIIV 124 Query: 123 ALQTIWSSGVGGSVGLEAGYTQLSSGLAASLGRGFHLRRNDQRIMVGCGAAAAIAGAFSA 182 ALQTIWSSGVG SVG EAGYTQ+SSG A+ LG+ FHLRR+DQRI+VGCG+AAAIAGAF A Sbjct: 125 ALQTIWSSGVGASVGTEAGYTQISSGTASVLGQAFHLRRSDQRILVGCGSAAAIAGAFGA 184 Query: 183 PLAGAFYAFELVIGGYTPASLTPVGVAAVTGYFVAHAFEPLPLGVGVGTVGDVLGRDLAI 242 PLAGAFYAFEL++ GYTPASL P+GVA++TGY VAH P G+ + VG V +D+ I Sbjct: 185 PLAGAFYAFELIVSGYTPASLAPIGVASITGYAVAHQLAPTVPGIILSQVGTVTSQDIVI 244 Query: 243 AALLGMVAALTGIAIMRGVTLCEALLTKSRLWPPLRPALGGLAVGAMALLTPQVMSSGHG 302 A LG + AL GIA+MR V + + ++++SRLW PLRP +GGL +G AL PQVMSSGHG Sbjct: 245 AVALGFLCALAGIAVMRAVAVWDTVMSRSRLWLPLRPTIGGLVMGLFALQAPQVMSSGHG 304 Query: 303 ALRFAGIVAMPLAFIAGVFALKAVASIVSLGSGFRGGLFFATLFMGALGGRLFAAGVDIV 362 AL +V +P+ +A +FALK +AS++SLGSGFRGGLFF++L GALGG LFAA ++ + Sbjct: 305 ALHTTNVVTLPVLMVAFLFALKILASVISLGSGFRGGLFFSSLLSGALGGHLFAAAMNTL 364 Query: 363 WPGLNLDPNAYAVIGMSALSASVIGGPLTMSFIALESTGNLWLTTAVLVAVIISTTITRE 422 P L+LD + YAVIGMSALSA+V+GGPLTM+FIALE+TGNLWLTTAVL+AVI+ST +TRE Sbjct: 365 VPSLHLDISVYAVIGMSALSAAVVGGPLTMTFIALETTGNLWLTTAVLIAVIVSTQVTRE 424 Query: 423 LFGYSFATWRLHLRGETIRSAAD 445 LFGYSFATWR HLRGETIRSAAD Sbjct: 425 LFGYSFATWRFHLRGETIRSAAD 447 >ref|YP_002288308.1| voltage gated chloride channel family protein [Oligotropha carboxidovorans OM5] gb|ACI92443.1| voltage gated chloride channel family protein [Oligotropha carboxidovorans OM5] Length = 598 Score = 524 bits (1350), Expect = e-146, Method: Compositional matrix adjust. Identities = 302/439 (68%), Positives = 364/439 (82%) Query: 7 YLEAPRRLRAFVRAHETSLVVLAVLIGAIGGLVVAVMSGLVTVLHSVLFNLPHGDRLSSQ 66 YLEAPRRLRA VRA E+ L+VLA LIG GGL+VA+MS V+ LH LFNLPHG+RLSSQ Sbjct: 19 YLEAPRRLRALVRARESGLIVLAALIGIAGGLLVALMSHAVSFLHEALFNLPHGERLSSQ 78 Query: 67 PSIDPVRALVVPTIGGLLLGFAFLLLLRVRPAREIDPIEANALYGGRMSFRGSVIVALQT 126 ++P+RAL+VP++GGL+ G L L R RP RE+DPIEANAL+GG+MS RGS+IVALQT Sbjct: 79 SVLEPLRALLVPSLGGLIFGLGLLALHRWRPGREVDPIEANALHGGQMSLRGSIIVALQT 138 Query: 127 IWSSGVGGSVGLEAGYTQLSSGLAASLGRGFHLRRNDQRIMVGCGAAAAIAGAFSAPLAG 186 +WSSGVG SVG EAGYTQ+SSG+A+SLG+ FHLRR+DQRI++GCG+AAAIAGAF APLAG Sbjct: 139 VWSSGVGASVGTEAGYTQISSGVASSLGKAFHLRRSDQRILLGCGSAAAIAGAFGAPLAG 198 Query: 187 AFYAFELVIGGYTPASLTPVGVAAVTGYFVAHAFEPLPLGVGVGTVGDVLGRDLAIAALL 246 AFYAFEL+I GYTPASL P+GVAA+TGY VAH P G+ + VG+V +D+AIA L Sbjct: 199 AFYAFELIISGYTPASLAPIGVAAITGYAVAHQLAPTVPGIILSQVGEVTSQDIAIALAL 258 Query: 247 GMVAALTGIAIMRGVTLCEALLTKSRLWPPLRPALGGLAVGAMALLTPQVMSSGHGALRF 306 G+++AL GIA+MR V + + +T+ RLW PLRPA+GGL VG AL PQVMSSGHGAL Sbjct: 259 GLLSALVGIAVMRSVAIWDTAMTRMRLWLPLRPAIGGLIVGVFALHAPQVMSSGHGALHT 318 Query: 307 AGIVAMPLAFIAGVFALKAVASIVSLGSGFRGGLFFATLFMGALGGRLFAAGVDIVWPGL 366 +VA+P+ +A +FALK +ASIVSLGSGFRGGLFF++L GALGG LFAA ++ + P L Sbjct: 319 TNLVALPVLTVATLFALKILASIVSLGSGFRGGLFFSSLLSGALGGHLFAAAMNTLVPTL 378 Query: 367 NLDPNAYAVIGMSALSASVIGGPLTMSFIALESTGNLWLTTAVLVAVIISTTITRELFGY 426 +LD + YAVIGMSA SASV+GGPLTM+FIALE+TGNLWLTTAVL+AVI+ST +TRELFGY Sbjct: 379 HLDISVYAVIGMSAFSASVVGGPLTMAFIALETTGNLWLTTAVLIAVIVSTQVTRELFGY 438 Query: 427 SFATWRLHLRGETIRSAAD 445 SFATWR HLRGETIRSAAD Sbjct: 439 SFATWRFHLRGETIRSAAD 457 >ref|YP_004633599.1| chloride channel protein [Oligotropha carboxidovorans OM5] gb|AEI03781.1| chloride channel protein [Oligotropha carboxidovorans OM4] gb|AEI07358.1| chloride channel protein [Oligotropha carboxidovorans OM5] Length = 588 Score = 523 bits (1347), Expect = e-146, Method: Compositional matrix adjust. Identities = 302/439 (68%), Positives = 364/439 (82%) Query: 7 YLEAPRRLRAFVRAHETSLVVLAVLIGAIGGLVVAVMSGLVTVLHSVLFNLPHGDRLSSQ 66 YLEAPRRLRA VRA E+ L+VLA LIG GGL+VA+MS V+ LH LFNLPHG+RLSSQ Sbjct: 9 YLEAPRRLRALVRARESGLIVLAALIGIAGGLLVALMSHAVSFLHEALFNLPHGERLSSQ 68 Query: 67 PSIDPVRALVVPTIGGLLLGFAFLLLLRVRPAREIDPIEANALYGGRMSFRGSVIVALQT 126 ++P+RAL+VP++GGL+ G L L R RP RE+DPIEANAL+GG+MS RGS+IVALQT Sbjct: 69 SVLEPLRALLVPSLGGLIFGLGLLALHRWRPGREVDPIEANALHGGQMSLRGSIIVALQT 128 Query: 127 IWSSGVGGSVGLEAGYTQLSSGLAASLGRGFHLRRNDQRIMVGCGAAAAIAGAFSAPLAG 186 +WSSGVG SVG EAGYTQ+SSG+A+SLG+ FHLRR+DQRI++GCG+AAAIAGAF APLAG Sbjct: 129 VWSSGVGASVGTEAGYTQISSGVASSLGKAFHLRRSDQRILLGCGSAAAIAGAFGAPLAG 188 Query: 187 AFYAFELVIGGYTPASLTPVGVAAVTGYFVAHAFEPLPLGVGVGTVGDVLGRDLAIAALL 246 AFYAFEL+I GYTPASL P+GVAA+TGY VAH P G+ + VG+V +D+AIA L Sbjct: 189 AFYAFELIISGYTPASLAPIGVAAITGYAVAHQLAPTVPGIILSQVGEVTSQDIAIALAL 248 Query: 247 GMVAALTGIAIMRGVTLCEALLTKSRLWPPLRPALGGLAVGAMALLTPQVMSSGHGALRF 306 G+++AL GIA+MR V + + +T+ RLW PLRPA+GGL VG AL PQVMSSGHGAL Sbjct: 249 GLLSALVGIAVMRSVAIWDTAMTRMRLWLPLRPAIGGLIVGVFALHAPQVMSSGHGALHT 308 Query: 307 AGIVAMPLAFIAGVFALKAVASIVSLGSGFRGGLFFATLFMGALGGRLFAAGVDIVWPGL 366 +VA+P+ +A +FALK +ASIVSLGSGFRGGLFF++L GALGG LFAA ++ + P L Sbjct: 309 TNLVALPVLTVATLFALKILASIVSLGSGFRGGLFFSSLLSGALGGHLFAAAMNTLVPTL 368 Query: 367 NLDPNAYAVIGMSALSASVIGGPLTMSFIALESTGNLWLTTAVLVAVIISTTITRELFGY 426 +LD + YAVIGMSA SASV+GGPLTM+FIALE+TGNLWLTTAVL+AVI+ST +TRELFGY Sbjct: 369 HLDISVYAVIGMSAFSASVVGGPLTMAFIALETTGNLWLTTAVLIAVIVSTQVTRELFGY 428 Query: 427 SFATWRLHLRGETIRSAAD 445 SFATWR HLRGETIRSAAD Sbjct: 429 SFATWRFHLRGETIRSAAD 447 >ref|YP_002421772.1| chloride channel core [Methylobacterium chloromethanicum CM4] gb|ACK83844.1| Chloride channel core [Methylobacterium chloromethanicum CM4] Length = 614 Score = 386 bits (991), Expect = e-105, Method: Compositional matrix adjust. Identities = 245/473 (51%), Positives = 307/473 (64%), Gaps = 42/473 (8%) Query: 3 TTSSYLEAPRRLRAFVRAHETSLVVLAVLIGAIGGLVVAVMSGLVTVLHSVLFNLPHGDR 62 T + +++AP RLRA+VR +E +LV LA + G +GGLVV MSG L +L+ LP G R Sbjct: 9 TAAPFVQAPVRLRAWVRGNEIALVGLAAVTGCVGGLVVVAMSGASQFLREILYRLPAGQR 68 Query: 63 LSSQPSIDPVRALVVPTIGGLLLGFAFLLLL----------------RVRPARE-IDPIE 105 LSS + PT G L+G FL R + R +DPIE Sbjct: 69 LSSLTDL--------PTEG--LMGAPFLAPALGGAILGLLLLISTRGRGKAKRAAVDPIE 118 Query: 106 ANALYGGRMSFRGSVIVALQTIWSSGVGGSVGLEAGYTQLSSGLAASLGRGFHLRRNDQR 165 ANAL+GGRMS RGS+ VA Q + S+G G SVGLEAGYTQ++SG+A+ LG F +RR+D R Sbjct: 119 ANALHGGRMSLRGSLDVAAQNVVSNGCGASVGLEAGYTQVASGIASRLGIAFEMRRSDLR 178 Query: 166 IMVGCGAAAAIAGAFSAPLAGAFYAFELVIGGYTPASLTPVGVAAVTGYFVAHAFEPLPL 225 +VGCG+AAAIA AF +PL GAFYAFEL+IG YT A+L PV VAA+ G VA A P P Sbjct: 179 TLVGCGSAAAIAAAFGSPLTGAFYAFELIIGTYTIATLAPVVVAALCGSLVARALAPQPA 238 Query: 226 GVGVGTVGDVLGRDLAIAA-------LLGMVAALTGIAIMRGVTLCEALLTKSRLWPPLR 278 V VG + L ++ A+ A +LG+V A G+ +MRGVTL E SRL PLR Sbjct: 239 LVDVGALA--LAKNTALGAGDTLPCLVLGLVCAGLGVLLMRGVTLVEQGFRASRLPAPLR 296 Query: 279 PALGGLAVGAMAL-LTPQVMSSGHGALR--FAGIVAMPLAFIAGVFALKAVASIVSLGSG 335 P LGGL VG +AL TPQV+S+GHGAL A L +AG+ KA AS +S+GSG Sbjct: 297 PVLGGLCVGGLALATTPQVLSAGHGALHITLAPEATAGLGALAGLIVAKAAASAMSIGSG 356 Query: 336 FRGGLFFATLFMGALGGRLFAAGVDIVWPGLN---LDPNAYAVIGMSALSASVIGGPLTM 392 FRGGLFFA+LF+GAL GRLFA ++WPG L P YAVIGMSA + +++GGPLTM Sbjct: 357 FRGGLFFASLFLGALVGRLFAGLAPMIWPGFEGFGLAPVGYAVIGMSAFAVAIVGGPLTM 416 Query: 393 SFIALESTGNLWLTTAVLVAVIISTTITRELFGYSFATWRLHLRGETIRSAAD 445 +F+ALE TG+ +T VL +VI S+ R+ FGYSFATWR HLRGE+IRSA D Sbjct: 417 TFLALEMTGSFPVTGLVLASVIASSLTVRKTFGYSFATWRFHLRGESIRSAHD 469