BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0102.0006.p (391 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_767212.1| DNA topoisomerase I [Bradyrhizobium japonicum U... 667 0.0 ref|YP_001236532.1| hypothetical protein BBta_0334 [Bradyrhizobi... 613 e-173 ref|YP_001202546.1| putative -like DNA topoisomerase I [Bradyrhi... 612 e-173 ref|ZP_08627925.1| DNA topoisomerase IB [Bradyrhizobiaceae bacte... 517 e-144 ref|YP_001525200.1| topoisomerase IB [Azorhizobium caulinodans O... 334 2e-89 >ref|NP_767212.1| DNA topoisomerase I [Bradyrhizobium japonicum USDA 110] dbj|BAC45837.1| bll0572 [Bradyrhizobium japonicum USDA 110] Length = 389 Score = 667 bits (1722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 337/390 (86%), Positives = 354/390 (90%), Gaps = 5/390 (1%) Query: 1 MDQQNIAAVRPVSADPA-VALAKALGQWPKQTPRAKLRSPSTLKLDAPAAIRTTVEELAR 59 MDQQN+ +RP SADPA VALAKALGQWPK +L SP + APAA TVE LAR Sbjct: 2 MDQQNLGTMRPASADPAAVALAKALGQWPKPAVSGRL-SPKKVFDTAPAA---TVEALAR 57 Query: 60 DLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVRYSSDP 119 +LGLRLGDQNELTIRRIKRGK YSFVR NG+ +RDARTIRRLHAMAVPPAYR+VRYS+DP Sbjct: 58 ELGLRLGDQNELTIRRIKRGKGYSFVRPNGAHIRDARTIRRLHAMAVPPAYREVRYSADP 117 Query: 120 SSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLSGDEPTR 179 S+HLQAVGRDAAGRLQYRYHADWEKVRE RKAHRL KLV ALPKIRRKVSAFLSGDEPTR Sbjct: 118 STHLQAVGRDAAGRLQYRYHADWEKVREHRKAHRLEKLVGALPKIRRKVSAFLSGDEPTR 177 Query: 180 EFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKGGKAVR 239 EFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDD VLTFKAKGGKAVR Sbjct: 178 EFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDCFVLTFKAKGGKAVR 237 Query: 240 KECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKISLKDFRT 299 KECDAAKLVRAIGILRGVPGKRMFQY D G+VRA +TT VNAFLREIAGIKISLKDFRT Sbjct: 238 KECDAAKLVRAIGILRGVPGKRMFQYRDAYGIVRAVNTTQVNAFLREIAGIKISLKDFRT 297 Query: 300 LMASAVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTIVTAFED 359 LMASAVVVESLSRITPATSQRGRK+QVLDAIRAAAD+LSNTPAICRKSYVHDTIVTAFED Sbjct: 298 LMASAVVVESLSRITPATSQRGRKKQVLDAIRAAADKLSNTPAICRKSYVHDTIVTAFED 357 Query: 360 GILERFAATMGGYRTQSKREQLLAQVVMAA 389 GILERFAATM G R+Q++REQLLAQVV A Sbjct: 358 GILERFAATMKGQRSQARREQLLAQVVATA 387 >ref|YP_001236532.1| hypothetical protein BBta_0334 [Bradyrhizobium sp. BTAi1] gb|ABQ32626.1| hypothetical protein BBta_0334 [Bradyrhizobium sp. BTAi1] Length = 395 Score = 613 bits (1581), Expect = e-173, Method: Compositional matrix adjust. Identities = 314/397 (79%), Positives = 343/397 (86%), Gaps = 11/397 (2%) Query: 2 DQQNIAAVRPVSADPAVALAKALGQWPKQTPRAKLRSPSTLK-------LDAPAAIRTTV 54 DQQN + R AD VALAKALGQWP + PR KL TLK +A A +T+V Sbjct: 3 DQQNFESSRVGPADAEVALAKALGQWPPKPPRRKL----TLKDLAHTKAANAQANAKTSV 58 Query: 55 EELARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQVR 114 EELA+ +GL+LGDQ ELTIRRIKRGK+YSF+RANG+ +R A TIRRLHAMAVPPAYR VR Sbjct: 59 EELAQRIGLKLGDQTELTIRRIKRGKSYSFIRANGTPIRHAGTIRRLHAMAVPPAYRDVR 118 Query: 115 YSSDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLSG 174 Y+ DPS HLQAVG DAAGRLQYRYHADWEKVRE RKAHRLAKLV ALP+IRR VS L+G Sbjct: 119 YAPDPSLHLQAVGIDAAGRLQYRYHADWEKVREHRKAHRLAKLVGALPRIRRAVSKNLAG 178 Query: 175 DEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAKG 234 DEPTREFAL+AVIELIARTAIRPGNESYARLNGTRGATTLLKSNV LEDD LVLTFKAKG Sbjct: 179 DEPTREFALAAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVILEDDGLVLTFKAKG 238 Query: 235 GKAVRKECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKISL 294 GKAVRKECDAAKLVRA+GILRG+PG+RMFQY D GVVRA STT VNAFLREIAGIKISL Sbjct: 239 GKAVRKECDAAKLVRAVGILRGLPGRRMFQYRDAQGVVRAVSTTQVNAFLREIAGIKISL 298 Query: 295 KDFRTLMASAVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTIV 354 KDFRTLMASAV +E+LSRITPA S RGRK+Q+L+AIRAAAD+L+NTPAICRKSYVHDTIV Sbjct: 299 KDFRTLMASAVALETLSRITPAASARGRKKQILEAIRAAADELTNTPAICRKSYVHDTIV 358 Query: 355 TAFEDGILERFAATMGGYRTQSKREQLLAQVVMAAGA 391 TAFEDGILERFAATM G R+Q++REQLLAQVVMA A Sbjct: 359 TAFEDGILERFAATMKGQRSQTRREQLLAQVVMAVAA 395 >ref|YP_001202546.1| putative -like DNA topoisomerase I [Bradyrhizobium sp. ORS 278] emb|CAL74300.1| conserved hypothetical protein; putative eukaryotic-like DNA topoisomerase I [Bradyrhizobium sp. ORS 278] Length = 395 Score = 612 bits (1579), Expect = e-173, Method: Compositional matrix adjust. Identities = 310/398 (77%), Positives = 341/398 (85%), Gaps = 11/398 (2%) Query: 1 MDQQNIAAVRPVSADPAVALAKALGQWPKQTPRAKLRSPSTLK-------LDAPAAIRTT 53 MDQQN + R AD VALAKALGQWP + R KL TLK A A +TT Sbjct: 2 MDQQNFESPRVGPADAEVALAKALGQWPPKPGRRKL----TLKDLAHAKTAHAQANAKTT 57 Query: 54 VEELARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQV 113 VEELA+ +GL+LGDQ+ELTIRRIKRGK+YSF+RANG+++R A TIRRLHAMAVPPAYR+V Sbjct: 58 VEELAKRIGLKLGDQSELTIRRIKRGKSYSFIRANGTQIRHAGTIRRLHAMAVPPAYREV 117 Query: 114 RYSSDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLS 173 RY+ DPS HLQAVG DAAGRLQYRYHADWEKVRE RKAHRLAKLV ALP+IRR VS L+ Sbjct: 118 RYAPDPSLHLQAVGIDAAGRLQYRYHADWEKVREHRKAHRLAKLVGALPRIRRAVSKHLA 177 Query: 174 GDEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAK 233 GD PTREFAL+AVIELIARTAIRPG+ESYARLNGTRGA TLLKSNV LEDD LVLTFKAK Sbjct: 178 GDAPTREFALAAVIELIARTAIRPGSESYARLNGTRGAATLLKSNVLLEDDCLVLTFKAK 237 Query: 234 GGKAVRKECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKIS 293 GGKAVRKECDAAKLVRA+G+LRG+PG+RMFQY D GVVRA STT VNAFLREIAGIKIS Sbjct: 238 GGKAVRKECDAAKLVRAVGVLRGLPGRRMFQYRDAQGVVRAVSTTQVNAFLREIAGIKIS 297 Query: 294 LKDFRTLMASAVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTI 353 KDFRTLMASAV +E+LSRITPA S RGRK+Q+L+AIRAAAD+L+NTPAICRKSYVHDTI Sbjct: 298 AKDFRTLMASAVALETLSRITPAESARGRKKQILEAIRAAADELTNTPAICRKSYVHDTI 357 Query: 354 VTAFEDGILERFAATMGGYRTQSKREQLLAQVVMAAGA 391 VTAFEDGILERFAATM G R+Q+KREQLLAQVV A A Sbjct: 358 VTAFEDGILERFAATMKGQRSQTKREQLLAQVVTAVSA 395 >ref|ZP_08627925.1| DNA topoisomerase IB [Bradyrhizobiaceae bacterium SG-6C] gb|EGP09454.1| DNA topoisomerase IB [Bradyrhizobiaceae bacterium SG-6C] Length = 367 Score = 517 bits (1331), Expect = e-144, Method: Compositional matrix adjust. Identities = 250/340 (73%), Positives = 290/340 (85%) Query: 52 TTVEELARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYR 111 ++V L + LGL++GDQ E IRR+KRGK Y FVRA G VRDA TIRR +AMAVPPAY Sbjct: 28 SSVRRLTKKLGLKIGDQMEFDIRRVKRGKGYCFVRAGGKLVRDAATIRRFNAMAVPPAYV 87 Query: 112 QVRYSSDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAF 171 VRY+SDP SHLQAVGRDAAGRLQYRYH DWEKVRE RKA RLA+L+ ALPKIRR V+ Sbjct: 88 DVRYASDPRSHLQAVGRDAAGRLQYRYHPDWEKVRETRKARRLARLIEALPKIRRSVAQH 147 Query: 172 LSGDEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFK 231 L D PTREF L+AVIEL+ARTAIRPGNESYARLNGTRGATTLLKSNV +EDD +VL+F+ Sbjct: 148 LGNDAPTREFTLAAVIELVARTAIRPGNESYARLNGTRGATTLLKSNVMIEDDCIVLSFR 207 Query: 232 AKGGKAVRKECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIK 291 AKG KAVRKEC+AA+LVRA+ LR +PG+R+FQY D GVVR +T VNAFLREIAG++ Sbjct: 208 AKGNKAVRKECNAARLVRAVETLRALPGRRLFQYRDDHGVVRTVNTAQVNAFLREIAGLR 267 Query: 292 ISLKDFRTLMASAVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHD 351 ISLKDFRTLMASA V+ESLSR+ PA S RGR++QVL+A+RAAAD+LSNTPAICRKSYVHD Sbjct: 268 ISLKDFRTLMASAAVLESLSRMVPAESARGRRKQVLEAVRAAADELSNTPAICRKSYVHD 327 Query: 352 TIVTAFEDGILERFAATMGGYRTQSKREQLLAQVVMAAGA 391 TIVTAFEDGILERFAAT+ G R+ ++REQ+LA+VV+ A A Sbjct: 328 TIVTAFEDGILERFAATLKGCRSPARREQILAKVVLTAAA 367 >ref|YP_001525200.1| topoisomerase IB [Azorhizobium caulinodans ORS 571] dbj|BAF88282.1| topoisomerase IB [Azorhizobium caulinodans ORS 571] Length = 381 Score = 334 bits (856), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 174/333 (52%), Positives = 231/333 (69%) Query: 54 VEELARDLGLRLGDQNELTIRRIKRGKTYSFVRANGSRVRDARTIRRLHAMAVPPAYRQV 113 V+E+AR GL L DQ+ L + R K G+ Y + A+G+ + D T RL ++AVPPA++QV Sbjct: 29 VKEMARAAGLVLVDQSALRLTRRKAGRGYCYFDASGTLITDEPTRARLASLAVPPAHKQV 88 Query: 114 RYSSDPSSHLQAVGRDAAGRLQYRYHADWEKVREQRKAHRLAKLVAALPKIRRKVSAFLS 173 RY+ +P +HLQAVG DAAGRLQYRYH WE VRE+ KA RLA+ AL IRR V+ L Sbjct: 89 RYAENPRAHLQAVGYDAAGRLQYRYHPRWELVRERCKAERLARFAEALSTIRRAVARDLG 148 Query: 174 GDEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSLVLTFKAK 233 +R FALSAV+EL+ TAIR G E YAR GTRGA TLLKSN+ E+ L+L+F+AK Sbjct: 149 SPPGSRTFALSAVVELVTLTAIRAGEEGYARARGTRGAATLLKSNLRSEEGQLILSFRAK 208 Query: 234 GGKAVRKECDAAKLVRAIGILRGVPGKRMFQYYDRSGVVRAASTTAVNAFLREIAGIKIS 293 GG+ + + + + L +PG+R+FQY D +G VR VNA+L+E+AG+ +S Sbjct: 209 GGRRTVCQITDPRFIVIVSSLSALPGRRLFQYRDDAGAVRTVRAGDVNAYLKEVAGVPVS 268 Query: 294 LKDFRTLMASAVVVESLSRITPATSQRGRKRQVLDAIRAAADQLSNTPAICRKSYVHDTI 353 LKDFRTL+ASA V++ L+R PA+S R R QV+ AIRAAA+ L+NTPAICRKSYV + Sbjct: 269 LKDFRTLVASASVLDILARAEPASSARARNAQVVAAIRAAAETLNNTPAICRKSYVMPAV 328 Query: 354 VTAFEDGILERFAATMGGYRTQSKREQLLAQVV 386 V AFEDG L RFAAT+ R+ +R +LA+++ Sbjct: 329 VAAFEDGTLSRFAATLKRCRSPIRRAGILAELI 361