BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0014.p (506 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08630008.1| phenylacetic acid degradation protein PaaN, r... 610 e-172 ref|YP_779930.1| bifunctional aldehyde dehydrogenase/enoyl-CoA h... 585 e-165 ref|YP_530576.1| bifunctional aldehyde dehydrogenase/enoyl-CoA h... 585 e-165 ref|ZP_01013473.1| phenylacetic acid degradation protein PaaZ [M... 487 e-135 ref|ZP_05078897.1| phenylacetic acid degradation protein paaN [R... 478 e-133 >ref|ZP_08630008.1| phenylacetic acid degradation protein PaaN, ring-opening aldehyde dehydrogenase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP07377.1| phenylacetic acid degradation protein PaaN, ring-opening aldehyde dehydrogenase [Bradyrhizobiaceae bacterium SG-6C] Length = 677 Score = 610 bits (1572), Expect = e-172, Method: Compositional matrix adjust. Identities = 306/428 (71%), Positives = 337/428 (78%), Gaps = 27/428 (6%) Query: 1 MKLDSFVRGKWSGPGEPAAEIHSAVTGDVVAVTTGSDLDMREILAYGRAVGGKHLGKLTF 60 MKLDS+V G+W+ P E+ SAVTGDVVA LDMR ILA+ R GGK+L +LTF Sbjct: 1 MKLDSYVNGQWTAPSGDFTEVRSAVTGDVVAEAMTGGLDMRGILAFARDTGGKNLRRLTF 60 Query: 61 HQRAGLLKALADYLTKHKDRLYKLSFHAGATRTDAVIDVDGGIGTLLAYAGKGRRELPNS 120 HQRA +LK LA YLT+ KD+LYKLSF GATRTD +IDVDGGIGTL YAGKGRRELPNS Sbjct: 61 HQRADMLKKLAQYLTERKDQLYKLSFQTGATRTDNLIDVDGGIGTLFVYAGKGRRELPNS 120 Query: 121 KFLLDGNVEGLSKGGAFAGQHIVTPLHGVAVHINAFNFPCWGMLEKLAPALLAGVPVVTK 180 FLLDG VE + K G FAGQHI+T L GVAVHINAFNFPCWG+LEKLAPALLAGVPVV+K Sbjct: 121 TFLLDGAVEPMGKAGTFAGQHIITSLQGVAVHINAFNFPCWGLLEKLAPALLAGVPVVSK 180 Query: 181 PATVTSYVAYELVRIITESGVLPEGALQFIVGSTGDLLDHLTCQDVVSFTGSADTSQKLQ 240 PATVTSYVA+ L R+I ESG+LPEGA QFIVGSTGDL DHLTCQDVVSFTGSADTS+KLQ Sbjct: 181 PATVTSYVAHALARMIAESGILPEGAFQFIVGSTGDLFDHLTCQDVVSFTGSADTSEKLQ 240 Query: 241 RHPVIAREAVRFIAERDSLNAAILAPDAVPGSPEFDLFVKEVAKEMTVKAGQKCTAIRRA 300 RHPVIAREAVRF+AERDSLNAAILAPD+ PG+PEFDLF+KEVAKEMTVKAGQKCTAIRR Sbjct: 241 RHPVIAREAVRFVAERDSLNAAILAPDSGPGTPEFDLFIKEVAKEMTVKAGQKCTAIRRV 300 Query: 301 LVPSAQLDAVITALRDRLAKVTIGNPENDKVRMGPLVGLGQRSDVLAHVAKLRAEAELVA 360 L P + +DAVI ALR+RL KV IGNPE D VRMGP+VGL QR DVL HV LR EA+LVA Sbjct: 301 LAPMSHVDAVIGALRERLDKVVIGNPEQDNVRMGPVVGLEQRRDVLNHVKALRTEAQLVA 360 Query: 361 GDP---------------------------XATAVHEIEAFGPVSTVMGYDDLDQAIALT 393 GDP AT VH IEAFGPVSTVMGY DLD+A+ LT Sbjct: 361 GDPDNFDVQGADVVRGAFVPPILLHCANPAQATKVHSIEAFGPVSTVMGYSDLDEAVTLT 420 Query: 394 NRGGGRXI 401 NRGGG + Sbjct: 421 NRGGGSLV 428 >ref|YP_779930.1| bifunctional aldehyde dehydrogenase/enoyl-CoA hydratase [Rhodopseudomonas palustris BisA53] gb|ABJ04950.1| phenylacetic acid degradation protein paaN [Rhodopseudomonas palustris BisA53] Length = 699 Score = 585 bits (1509), Expect = e-165, Method: Compositional matrix adjust. Identities = 306/428 (71%), Positives = 344/428 (80%), Gaps = 27/428 (6%) Query: 1 MKLDSFVRGKWSGPGEPAAEIHSAVTGDVVAVTTGSDLDMREILAYGRAVGGKHLGKLTF 60 MKL+SFV G+W+ PG E+ AVTG+VVA T LDMR +LA+ R G L +LTF Sbjct: 25 MKLESFVGGQWTAPGRDRIELLGAVTGEVVAEATSGGLDMRALLAFARDKSGPALRRLTF 84 Query: 61 HQRAGLLKALADYLTKHKDRLYKLSFHAGATRTDAVIDVDGGIGTLLAYAGKGRRELPNS 120 HQRA LLK LA YL + KD+LY+LSF AG TRTD++IDVDGGIGTL+AYAGKGRRELPN+ Sbjct: 85 HQRAELLKKLAQYLAERKDQLYQLSFLAGCTRTDSLIDVDGGIGTLIAYAGKGRRELPNA 144 Query: 121 KFLLDGNVEGLSKGGAFAGQHIVTPLHGVAVHINAFNFPCWGMLEKLAPALLAGVPVVTK 180 FLLDG VE LSKGG+F G+HIVT LHGVAVHINAFNFPCWG+LEKLAPALLAGVPVV+K Sbjct: 145 TFLLDGGVEPLSKGGSFVGRHIVTSLHGVAVHINAFNFPCWGLLEKLAPALLAGVPVVSK 204 Query: 181 PATVTSYVAYELVRIITESGVLPEGALQFIVGSTGDLLDHLTCQDVVSFTGSADTSQKLQ 240 PATVTSYVA+ LVR+I ES +LP+GALQFIVGST DL DHLTCQDVVSFTGSA TS+KLQ Sbjct: 205 PATVTSYVAHALVRMIVESNILPDGALQFIVGSTDDLFDHLTCQDVVSFTGSAATSEKLQ 264 Query: 241 RHPVIAREAVRFIAERDSLNAAILAPDAVPGSPEFDLFVKEVAKEMTVKAGQKCTAIRRA 300 RHPVIAREAVRFIAERDSLNAAILAPDA PG+PEFDLFVKEVAKEMTVKAGQKCTAIRRA Sbjct: 265 RHPVIAREAVRFIAERDSLNAAILAPDAAPGTPEFDLFVKEVAKEMTVKAGQKCTAIRRA 324 Query: 301 LVPSAQLDAVITALRDRLAKVTIGNPENDKVRMGPLVGLGQRSDVLAHVAKLRAEAELVA 360 LVP+AQLDA I ALR+RL+KVT+GNPE + VRMGPLVGL QR DVL +A+LRAEA LVA Sbjct: 325 LVPAAQLDAAIAALRERLSKVTVGNPELETVRMGPLVGLDQRRDVLEQLARLRAEANLVA 384 Query: 361 GDPX---------------------------ATAVHEIEAFGPVSTVMGYDDLDQAIALT 393 GDP A AVHE+EAFGPV+T+M Y DL++AI L Sbjct: 385 GDPDHFAVEGADSSRGAFVPPLLLHCADPIRANAVHEVEAFGPVATIMPYRDLNEAIVLA 444 Query: 394 NRGGGRXI 401 NRGGG + Sbjct: 445 NRGGGSLV 452 >ref|YP_530576.1| bifunctional aldehyde dehydrogenase/enoyl-CoA hydratase [Rhodopseudomonas palustris BisB18] gb|ABD86257.1| Phenylacetic acid degradation protein paaN [Rhodopseudomonas palustris BisB18] Length = 674 Score = 585 bits (1507), Expect = e-165, Method: Compositional matrix adjust. Identities = 310/428 (72%), Positives = 341/428 (79%), Gaps = 28/428 (6%) Query: 1 MKLDSFVRGKWSGPGEPAAEIHSAVTGDVVAVTTGSDLDMREILAYGRAVGGKHLGKLTF 60 MKL+SFV+G+W G EI SAVTG+VVA T LD+R+ LAYGR GG HL +L+F Sbjct: 1 MKLESFVQGQWRA-GRDRIEIRSAVTGNVVAEATSGGLDLRQALAYGRDTGGAHLRRLSF 59 Query: 61 HQRAGLLKALADYLTKHKDRLYKLSFHAGATRTDAVIDVDGGIGTLLAYAGKGRRELPNS 120 HQRA LLK LA YL + K++LYKLSF GATR DA+IDVDGGIGTLL YAGKGRRELPN+ Sbjct: 60 HQRAELLKKLASYLGERKEQLYKLSFATGATRGDALIDVDGGIGTLLVYAGKGRRELPNA 119 Query: 121 KFLLDGNVEGLSKGGAFAGQHIVTPLHGVAVHINAFNFPCWGMLEKLAPALLAGVPVVTK 180 FLLDG VE LSKGG FAG+HIVTPL GVA+HINAFNFPCWG+LEKLAPALLAGVPV++K Sbjct: 120 TFLLDGAVEPLSKGGNFAGRHIVTPLRGVALHINAFNFPCWGLLEKLAPALLAGVPVISK 179 Query: 181 PATVTSYVAYELVRIITESGVLPEGALQFIVGSTGDLLDHLTCQDVVSFTGSADTSQKLQ 240 PATVT+YVA+ L R+I ES +LPEGALQF+VGST DL +HLTCQDVVSFTGSA TSQ LQ Sbjct: 180 PATVTAYVAHALARMIAESQILPEGALQFLVGSTDDLFEHLTCQDVVSFTGSAATSQALQ 239 Query: 241 RHPVIAREAVRFIAERDSLNAAILAPDAVPGSPEFDLFVKEVAKEMTVKAGQKCTAIRRA 300 RHPVIAREAVRFIAERDSLNAAILAPDA PG+PEFDLFVKEVAKEMTVKAGQKCTAIRRA Sbjct: 240 RHPVIAREAVRFIAERDSLNAAILAPDAAPGTPEFDLFVKEVAKEMTVKAGQKCTAIRRA 299 Query: 301 LVPSAQLDAVITALRDRLAKVTIGNPENDKVRMGPLVGLGQRSDVLAHVAKLRAEAELVA 360 L+P+A LDA I ALR RLA VTIGNPE D VRMGPLVGL QR DVLAHVA LR EAELVA Sbjct: 300 LLPAAHLDAAIAALRTRLASVTIGNPELDNVRMGPLVGLDQRRDVLAHVATLRQEAELVA 359 Query: 361 GDPX---------------------------ATAVHEIEAFGPVSTVMGYDDLDQAIALT 393 GDP ATAVHE+EAFGPV T+M Y DL +AI LT Sbjct: 360 GDPDNFEIEGADARRDAFLPPLLLHCADPARATAVHEVEAFGPVCTLMPYGDLAEAITLT 419 Query: 394 NRGGGRXI 401 NRGGG + Sbjct: 420 NRGGGSLV 427 >ref|ZP_01013473.1| phenylacetic acid degradation protein PaaZ [Maritimibacter alkaliphilus HTCC2654] gb|EAQ12715.1| phenylacetic acid degradation protein PaaZ [Rhodobacterales bacterium HTCC2654] Length = 678 Score = 487 bits (1254), Expect = e-135, Method: Compositional matrix adjust. Identities = 247/428 (57%), Positives = 297/428 (69%), Gaps = 27/428 (6%) Query: 1 MKLDSFVRGKWSGPGEPAAEIHSAVTGDVVAVTTGSDLDMREILAYGRAVGGKHLGKLTF 60 M + S+ G+W PG A I SAVTG+V+A LD++ +L + R VGG +L K+ F Sbjct: 6 MDVSSYAAGEWVKPGAGARNIASAVTGEVIATAGNDSLDVQAMLDHARNVGGPNLRKMGF 65 Query: 61 HQRAGLLKALADYLTKHKDRLYKLSFHAGATRTDAVIDVDGGIGTLLAYAGKGRRELPNS 120 H+RA +LKAL YLT+HK +LY +SF GAT+ D D+DGGIGTL +A KGRRELP+ Sbjct: 66 HERAKMLKALGQYLTEHKQKLYDISFATGATQGDHGFDIDGGIGTLYVFASKGRRELPDG 125 Query: 121 KFLLDGNVEGLSKGGAFAGQHIVTPLHGVAVHINAFNFPCWGMLEKLAPALLAGVPVVTK 180 LLDG+VE S+ G F GQHI TP+ GVAVHINA+NFP WGMLEKLAP LL G+P + K Sbjct: 126 HVLLDGDVEEFSRHGTFKGQHIYTPMQGVAVHINAYNFPVWGMLEKLAPTLLGGMPAIVK 185 Query: 181 PATVTSYVAYELVRIITESGVLPEGALQFIVGSTGDLLDHLTCQDVVSFTGSADTSQKLQ 240 PAT TSYV V+++ +SG+LPEGALQ + G TGD+LD LTCQDVVSFTGSADT+ KL+ Sbjct: 186 PATATSYVTELCVKLMLDSGLLPEGALQLVAGGTGDMLDRLTCQDVVSFTGSADTALKLR 245 Query: 241 RHPVIAREAVRFIAERDSLNAAILAPDAVPGSPEFDLFVKEVAKEMTVKAGQKCTAIRRA 300 P I + AVRF AE DSLN++IL PDA PG+PEFDLFVKEV +EMTVKAGQKCTAIRR Sbjct: 246 STPAILQNAVRFTAEADSLNSSILGPDAEPGTPEFDLFVKEVQREMTVKAGQKCTAIRRI 305 Query: 301 LVPSAQLDAVITALRDRLAKVTIGNPENDKVRMGPLVGLGQRSDVLAHVAKLRAEAELVA 360 L P + ++AVI ALR+RLAK IG+P N+ RMG LV GQR+DVL +A L EAE V Sbjct: 306 LAPESHVEAVIDALRERLAKNVIGDPANESTRMGALVSTGQRADVLEKIAILGTEAERVI 365 Query: 361 GDP---------------------------XATAVHEIEAFGPVSTVMGYDDLDQAIALT 393 GDP AT VHE EAFGPVST+MGY DLD AI L Sbjct: 366 GDPDNFEVHGADREKGAFLPPMLYHCANPDAATKVHETEAFGPVSTIMGYRDLDHAIDLA 425 Query: 394 NRGGGRXI 401 NRG G + Sbjct: 426 NRGQGSLV 433 >ref|ZP_05078897.1| phenylacetic acid degradation protein paaN [Rhodobacterales bacterium Y4I] gb|EDZ46876.1| phenylacetic acid degradation protein paaN [Rhodobacterales bacterium Y4I] Length = 676 Score = 478 bits (1231), Expect = e-133, Method: Compositional matrix adjust. Identities = 250/459 (54%), Positives = 307/459 (66%), Gaps = 33/459 (7%) Query: 1 MKLDSFVRGKWSGPGEPAAEIHSAVTGDVVAVTTGSDLDMREILAYGRAVGGKHLGKLTF 60 +++ SF G+W PG A I SA+TG+V+A LD++ +L Y R VGG +L KLTF Sbjct: 4 LEISSFAAGQWVAPGAGARNIASAITGEVIASAGNDALDVQAMLDYARTVGGPNLRKLTF 63 Query: 61 HQRAGLLKALADYLTKHKDRLYKLSFHAGATRTDAVIDVDGGIGTLLAYAGKGRRELPNS 120 H RA +LKALA +L +HK LY LSF+ GAT++D +ID+DGGIGT+ +A KGRRE+P+ Sbjct: 64 HDRARMLKALAGHLNQHKQALYDLSFNTGATQSDHMIDIDGGIGTMFVFASKGRREMPDG 123 Query: 121 KFLLDGNVEGLSKGGAFAGQHIVTPLHGVAVHINAFNFPCWGMLEKLAPALLAGVPVVTK 180 LDG+VE LS+ G F GQHI TPL GVAVHINAFNFP WGMLEKLAP LLAGVP + K Sbjct: 124 HVYLDGDVEQLSRNGTFLGQHICTPLRGVAVHINAFNFPVWGMLEKLAPTLLAGVPAIVK 183 Query: 181 PATVTSYVAYELVRIITESGVLPEGALQFIVGSTGDLLDHLTCQDVVSFTGSADTSQKLQ 240 PAT + YV V+++ +SG+LPEGALQ + G GD+LDHLT QDVVSFTGSA+T+ KL+ Sbjct: 184 PATNSCYVTELAVKLMLDSGILPEGALQLVSGGLGDMLDHLTMQDVVSFTGSANTALKLR 243 Query: 241 RHPVIAREAVRFIAERDSLNAAILAPDAVPGSPEFDLFVKEVAKEMTVKAGQKCTAIRRA 300 +PVI +VRF+AE+DSLNA+IL PDA PG+PEFDLFVKEV++EMT KAGQKCTAIRR Sbjct: 244 ANPVILENSVRFVAEQDSLNASILGPDAQPGTPEFDLFVKEVSREMTTKAGQKCTAIRRI 303 Query: 301 LVPSAQLDAVITALRDRLAKVTIGNPENDKVRMGPLVGLGQRSDVLAHVAKLRAEAELVA 360 + P AQ+DAVI AL RLAK IG+P + RMG LV Q+ DVL A + EAE V Sbjct: 304 IAPQAQVDAVIEALSARLAKTQIGDPRLETTRMGALVSNSQKRDVLEKAAIIGQEAERVF 363 Query: 361 GDP---------------------------XATAVHEIEAFGPVSTVMGYDDLDQAIALT 393 GDP A VH+ EAFGPVST+MGY DLD AI L Sbjct: 364 GDPENFSVDGADAEKGAFVPPMLFHCADPDNARRVHDTEAFGPVSTIMGYRDLDHAIDLA 423 Query: 394 NRGGGRXI-HLHARRPGCDR-----AGIWHRQFSRPRRD 426 NRG G + + P R AG +H + RD Sbjct: 424 NRGQGSLVASVITHDPAVAREVAIGAGAYHGRLYFNNRD 462