BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0098.0034.p (505 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ADP99864.1| long-chain-fatty-acid-CoA ligase [Marinobacter ad... 307 3e-81 ref|ZP_01893709.1| AMP-dependent synthetase and ligase [Marinoba... 305 9e-81 ref|ZP_07715953.1| AMP-binding enzyme [Aeromicrobium marinum DSM... 300 6e-79 ref|NP_884300.1| putative long-chain fatty-acid--CoA ligase [Bor... 299 8e-79 ref|YP_285561.1| AMP-dependent synthetase and ligase [Dechloromo... 298 2e-78 >gb|ADP99864.1| long-chain-fatty-acid-CoA ligase [Marinobacter adhaerens HP15] Length = 603 Score = 307 bits (786), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 169/403 (41%), Positives = 237/403 (58%), Gaps = 15/403 (3%) Query: 39 ITIPQLLRQRATMHGEAMALREKDYGIWNPYSWRHYYDTARAVALGLLSLGIKPGDRIAI 98 +T+ Q+LR A +A+ALR+KD+GIW YSW+ YY AR LGL ++G++PG +AI Sbjct: 12 LTLTQMLRAHARERPDALALRQKDFGIWQAYSWQDYYRRARHFGLGLRAMGLEPGGHVAI 71 Query: 99 AGENTPEWFYADLGAQMIGAVAVGIYPTNPWVELQYIVKHSGARIVVTGDQEQTDKVLDA 158 EN EW +G ++ + VG+YPT+PW E+ Y+++HS A V+ DQEQTDKVL+A Sbjct: 72 ISENRVEWVIGQMGIGLVQGICVGVYPTSPWNEVAYVLEHSDAEFVICEDQEQTDKVLEA 131 Query: 159 MAGNGGLPDLEAIVCIDMKGLRHYRQSELMSFAALCERGRAYAQQNPEADATLDRLISQG 218 LP L+ + IDMKGLR+Y + +F + RGR + Q+P +D L+ Sbjct: 132 WP---DLPKLKHCIAIDMKGLRYYPEPP-SAFEDIEARGREFETQHPN---LVDELLDSQ 184 Query: 219 APDDVCILVYTSGTTGPPKGAMLTHRNLVYAAHAYARTVGIADKPFEAVSYLPLCHVAER 278 DD +++YTSG+TG PKGAM++ NL AA + AD+ ++SYLPLCHVAE+ Sbjct: 185 QMDDTALMIYTSGSTGRPKGAMISWHNLHAAAPGLIELLQ-ADEHGSSLSYLPLCHVAEQ 243 Query: 279 CYGTVTHLVLGGTVSFAESIDTVAINIREIAPTFFVGVPRIYEKLQQGFLFRLGESGRLR 338 + + + +G TVSF ES+ T+ ++REIAPTFF+GVPRI+EKL ++ E+GRLR Sbjct: 244 AFTNIAPVYVGSTVSFGESLRTIQEDLREIAPTFFLGVPRIWEKLHSSIYIKIQETGRLR 303 Query: 339 QSFTRACLAWG-RTLSDRRQTGNDNW--LDRAAYGLLYLLMFRNLQRHLGFAHSRHRLCA 395 Q A W R S +W ++ YG+ Y L+ R LQ +G L Sbjct: 304 Q----ALFNWAIRVCSPMATKPRADWNLKEKCLYGISYWLVLRALQNFIGLRRCSIALTG 359 Query: 396 GASISPETLRFFDIIGRPVSQGYGLTESGGVAFIQTESHHRIG 438 A IS L FF IG P+ + YG TES GVA Q R+G Sbjct: 360 AAPISTGILEFFRTIGIPLVEVYGQTESTGVATAQPVDDVRLG 402 >ref|ZP_01893709.1| AMP-dependent synthetase and ligase [Marinobacter algicola DG893] gb|EDM48279.1| AMP-dependent synthetase and ligase [Marinobacter algicola DG893] Length = 600 Score = 305 bits (782), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 170/395 (43%), Positives = 235/395 (59%), Gaps = 15/395 (3%) Query: 39 ITIPQLLRQRATMHGEAMALREKDYGIWNPYSWRHYYDTARAVALGLLSLGIKPGDRIAI 98 +T+ Q+LR A +A+ALR+KD+GIW YSWR YY+ AR LGL ++G++ G +AI Sbjct: 9 LTLTQMLRAHAQERPDALALRQKDFGIWQAYSWRAYYERARHFGLGLRAMGLEKGGHVAI 68 Query: 99 AGENTPEWFYADLGAQMIGAVAVGIYPTNPWVELQYIVKHSGARIVVTGDQEQTDKVLDA 158 EN EW A +G M+ + VG+YPT+PW E+ Y+++HS A IVV DQEQTDKVL+A Sbjct: 69 ISENRVEWVIAQMGIGMVRGICVGVYPTSPWKEVAYVLEHSDASIVVCEDQEQTDKVLEA 128 Query: 159 MAGNGGLPDLEAIVCIDMKGLRHYRQSELMSFAALCERGRAYAQQNPEADATLDRLISQG 218 LP L+ + IDMKGLR+Y + +F + RGRA+ + +P +D L+ Sbjct: 129 WP---QLPQLKHAIAIDMKGLRYYPEPP-AAFEDIEARGRAFEKDHP---GLVDELLDGQ 181 Query: 219 APDDVCILVYTSGTTGPPKGAMLTHRNLVYAAHAYARTVGIADKPFEAVSYLPLCHVAER 278 DD +++YTSG+TG PKGAM++ NL+ AA + AD+ ++SYLPLCHVAE+ Sbjct: 182 RMDDTALMIYTSGSTGRPKGAMISWGNLLAAAPGLIELLQ-ADERGSSLSYLPLCHVAEQ 240 Query: 279 CYGTVTHLVLGGTVSFAESIDTVAINIREIAPTFFVGVPRIYEKLQQGFLFRLGESGRLR 338 + + G +VSF ES+ TV ++REIAPTFF+GVPRI+EKL ++ E+GR+R Sbjct: 241 AVTNIAPVYAGSSVSFGESLRTVQEDLREIAPTFFLGVPRIWEKLHSSIYIKIQETGRVR 300 Query: 339 QSFTRACLAWG-RTLSDRRQTGNDNW--LDRAAYGLLYLLMFRNLQRHLGFAHSRHRLCA 395 RA W R S W ++ Y L Y L+FR LQ +G + Sbjct: 301 ----RALFNWAIRACSPMATKSRAQWSLKEKGLYSLCYWLVFRALQNFIGLRRCTIAMTG 356 Query: 396 GASISPETLRFFDIIGRPVSQGYGLTESGGVAFIQ 430 A IS L FF IG P+ + YG TES GVA Q Sbjct: 357 AAPISTGILEFFRTIGIPLLEVYGQTESTGVATAQ 391 >ref|ZP_07715953.1| AMP-binding enzyme [Aeromicrobium marinum DSM 15272] gb|EFQ84543.1| AMP-binding enzyme [Aeromicrobium marinum DSM 15272] Length = 614 Score = 300 bits (767), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 161/408 (39%), Positives = 238/408 (58%), Gaps = 17/408 (4%) Query: 40 TIPQLLRQRATMHGEAMALREKDYGIWNPYSWRHYYDTARAVALGLLSLGIKPGDRIAIA 99 T+P L+R RA + +A+REK +GIW +W Y++ + VA GLL+LG++PGDRIA+ Sbjct: 3 TLPWLVRDRAARMPDRIAMREKSFGIWEEVTWAQYWERSTLVAHGLLALGVEPGDRIAVH 62 Query: 100 GENTPEWFYADLGAQMIGAVAVGIYPTNPWVELQYIVKHSGARIVVTGDQEQTDKVLDAM 159 EN EW Y D+G + A VG+YPTNP E+ ++++ SG+R++ DQEQ DK LD + Sbjct: 63 SENRREWLYCDIGITSVRAATVGLYPTNPSPEVLHVLRDSGSRVLFAEDQEQLDKALDVI 122 Query: 160 AGNGGLPDLEAIVCIDMKGLR-HYRQSELMSFAALCERGRAYAQQNPEADATLDRLISQG 218 LP LE IV ++ +G++ HY L+S+ G + Q+P +LD + Sbjct: 123 ---DELPHLERIVYLEPRGIQGHYTDGRLLSWEEFVALGEQHRSQHP---GSLDERLEAV 176 Query: 219 APDDVCILVYTSGTTGPPKGAMLTHRNLVYAAHAYARTVGIADKPFE----AVSYLPLCH 274 P D+ LVYTSGTTGPPKGAMLT N+ + P +SYLPLCH Sbjct: 177 EPSDLATLVYTSGTTGPPKGAMLTQANITFVLDTLQGQSAFVSPPANEKDLVLSYLPLCH 236 Query: 275 VAERCYGTVTHLVLGGTVSFAESIDTVAINIREIAPTFFVGVPRIYEKLQQGFLFRLGES 334 VAER + T + +G V+FAESIDTV +N+RE+ PT GVPRI+EK+ G R+ + Sbjct: 237 VAERVFTTWFNAAVGTQVNFAESIDTVQLNLREVQPTLVFGVPRIWEKIAAGVQVRMAGA 296 Query: 335 GRLRQSFTRACL---AW-GRTLSDRRQTGNDNWLDRAAYGLLYLLMFRNLQRHLGFAHSR 390 R++++ + +W GRTL +R+ G R AY + +++++R L+ +G A +R Sbjct: 297 SRIKRANYAVWMKVASWIGRTLVERQ--GKHTVGTRIAYAVGWVMLYRPLRERIGLAKTR 354 Query: 391 HRLCAGASISPETLRFFDIIGRPVSQGYGLTESGGVAFIQTESHHRIG 438 + A ISPE L FF +G P+ + YG+TE+ +A R+G Sbjct: 355 YAASGAAPISPEVLEFFMGLGLPMFEVYGMTENSAIATANMAGRVRVG 402 >ref|NP_884300.1| putative long-chain fatty-acid--CoA ligase [Bordetella parapertussis 12822] ref|NP_888833.1| long-chain fatty-acid--CoA ligase [Bordetella bronchiseptica RB50] emb|CAE37342.1| putative long-chain fatty-acid--CoA ligase [Bordetella parapertussis] emb|CAE32786.1| putative long-chain fatty-acid--CoA ligase [Bordetella bronchiseptica RB50] Length = 621 Score = 299 bits (765), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 172/405 (42%), Positives = 242/405 (59%), Gaps = 13/405 (3%) Query: 38 AITIPQLLRQRATMHGEAMALREKDYGIWNPYSWRHYYDTARAVALGLLSLGIKPGDRIA 97 A+T+PQ+LR++A +ALR+KDYGIW P W Y+ A VALGL + G++PG ++A Sbjct: 20 ALTLPQMLREQARRQPARIALRQKDYGIWQPIDWATYWRRACQVALGLRAAGLEPGGKVA 79 Query: 98 IAGENTPEWFYADLGAQMIGAVAVGIYPTNPWVELQYIVKHSGARIVVTGDQEQTDKVLD 157 I EN EW A +GA ++GAV VG+Y T+P E+ Y+++H+ +VV DQEQTDKVL+ Sbjct: 80 IIAENRLEWLLAQMGAGVLGAVPVGVYCTSPAAEVGYVLEHAEVDMVVCEDQEQTDKVLE 139 Query: 158 AMAGNGGLPDLEAIVCIDMKGLRHYRQSE---LMSFAALCERGRAYAQQNPEADATLDRL 214 A LP L IV ++ KGLR++ ++E ++SFA L RG AQ A L++ Sbjct: 140 VAA---RLPGLRRIVVMETKGLRNHAEAERARMISFAELEARG---AQCGAAEQARLEQA 193 Query: 215 ISQGAPDDVCILVYTSGTTGPPKGAMLTHRNLVYAAHAYARTVGIADKPFEAVSYLPLCH 274 + DDV +++YTSG+TG PKGAM+++RN+ A A + + D +SYLPLCH Sbjct: 194 LDAQTLDDVGLMIYTSGSTGKPKGAMISYRNMRGVAPGIADRLDM-DAGSVHLSYLPLCH 252 Query: 275 VAERCYGTVTHLVLGGTVSFAESIDTVAINIREIAPTFFVGVPRIYEKLQQGFLFRLGES 334 VAE+ T + LG V+F ESI TV ++RE+APT F+GVPRI+EKL ++ ES Sbjct: 253 VAEQMLSTFVPVYLGSQVNFGESIRTVQEDLREVAPTVFLGVPRIWEKLHAAISIKMQES 312 Query: 335 GRLRQ-SFTRACLAWGRTLSDRRQTGNDNWLDRAAYGLLYLLMFRNLQRHLGFAHSRHRL 393 GRLR+ + RA L R W +R YG Y L+ R L +G + + Sbjct: 313 GRLRRWLYARALRGCAPFLELSR--ARYGWRERLVYGWHYALILRALHNFVGLRRVKVAM 370 Query: 394 CAGASISPETLRFFDIIGRPVSQGYGLTESGGVAFIQTESHHRIG 438 A I PE LR+F +G P+ + YGLTES G+ F Q R+G Sbjct: 371 TGAAPIPPEVLRYFRTLGIPLIEVYGLTESTGMIFGQHPDRVRVG 415 >ref|YP_285561.1| AMP-dependent synthetase and ligase [Dechloromonas aromatica RCB] gb|AAZ47091.1| AMP-dependent synthetase and ligase [Dechloromonas aromatica RCB] Length = 610 Score = 298 bits (762), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 160/407 (39%), Positives = 247/407 (60%), Gaps = 19/407 (4%) Query: 39 ITIPQLLRQRATMHGEAMALREKDYGIWNPYSWRHYYDTARAVALGLLSLGIKPGDRIAI 98 +T+PQ+LRQRA +A+R+KD+GIWNP SW YY A LGL +LG++PG I + Sbjct: 8 LTLPQMLRQRAQQQPNRVAIRQKDFGIWNPISWDDYYRRAGHFGLGLCALGVQPGGHIGV 67 Query: 99 AGENTPEWFYADLGAQMIGAVAVGIYPTNPWVELQYIVKHSGARIVVTGDQEQTDKVLDA 158 EN EW A +GA ++GAV VG+YPT+P E+ Y+++H+ +V+ DQEQ+DKVL A Sbjct: 68 ISENRVEWVLAQMGAGLVGAVTVGVYPTSPTNEVSYVLEHADIDVVICEDQEQSDKVLAA 127 Query: 159 MAGNGGLPDLEAIVCIDMKGLRH---YRQSELMSFAALCERGRAYAQQNPEADATLDRLI 215 + LP L IV ++ KGLR+ + ++++F+ + G Y + +D ++ Sbjct: 128 I---DSLPRLRKIVVMEEKGLRNTPAEFRDKVLAFSEVEALGVDYEAVHR---TLIDEVL 181 Query: 216 SQGAPDDVCILVYTSGTTGPPKGAMLTHRNLVYAAHAYARTVGIADKPFEAVSYLPLCHV 275 ++ + DD+ +++YTSG+TG PKGAM+++ N+ A +G+ D+ +SYLPLCHV Sbjct: 182 AKQSLDDIGLMIYTSGSTGKPKGAMISYGNIRAVAPGIIERLGL-DEHSRHLSYLPLCHV 240 Query: 276 AERCYGTVTHLVLGGTVSFAESIDTVAINIREIAPTFFVGVPRIYEKLQQGFLFRLGESG 335 AE+ T T + LG V+F ES+ TV ++RE+APT F+GVPRI+EKL R+ E+G Sbjct: 241 AEQMLTTFTPIYLGSQVNFGESLRTVQEDLREVAPTLFLGVPRIWEKLHASISIRIHETG 300 Query: 336 RLRQ-SFTR---ACLAWGRTLSDRRQTGNDNWLDRAAYGLLYLLMFRNLQRHLGFAHSRH 391 R R+ F + AC + +R G ++ +G Y+L+ R LQ ++G +++ Sbjct: 301 RFRRWLFDKALVACETFACKAVHQRTLG-----EKLTFGFYYVLILRALQNYIGLRNAKV 355 Query: 392 RLCAGASISPETLRFFDIIGRPVSQGYGLTESGGVAFIQTESHHRIG 438 L A ISP + FF +G P+ + YGLTES G+ F Q H ++G Sbjct: 356 ALTGAAPISPAIISFFRALGVPLVEVYGLTESSGMIFGQRLDHVQLG 402