BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0097.0032.p (366 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001811919.1| type III effector Hrp-dependent outer [Burkh... 353 2e-95 ref|YP_001566657.1| Hrp-dependent type III effector protein [Del... 333 2e-89 ref|YP_001267183.1| type III effector Hrp-dependent outer [Pseud... 312 6e-83 ref|YP_003243071.1| Hrp-dependent type III effector protein [Pae... 239 7e-61 ref|ZP_02886747.1| type III effector Hrp-dependent protein [Burk... 239 7e-61 >ref|YP_001811919.1| type III effector Hrp-dependent outer [Burkholderia ambifaria MC40-6] gb|ACB67703.1| type III effector Hrp-dependent outer [Burkholderia ambifaria MC40-6] Length = 469 Score = 353 bits (906), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 176/338 (52%), Positives = 226/338 (66%) Query: 20 DPIPIVGGTPSLQRYCIFGNLFARSGTDGNIYRIDRHPIMSVHPVTPMNESDLAVHIGRQ 79 +P+PIV GTP+L+RYC FGNLFARSGTD YRIDRHPIMS HPVTPM E DL H+ Q Sbjct: 123 NPVPIVAGTPALKRYCAFGNLFARSGTDNKTYRIDRHPIMSAHPVTPMAEGDLLRHLALQ 182 Query: 80 AALEIEKLTLPFLDQDHARAAGRFAELAKCAPDAILLDSVNASQLTQVGRLIADRAAGNR 139 AL + LP L+ +++ ILLDSV LT+VGRL+ + A Sbjct: 183 TALPLSGFQLPNLEGTREEVDLAWSKAISTGDSGILLDSVTPRHLTEVGRLLDNYATAVG 242 Query: 140 SAFVVGPSGVEYALMQWWREAGIIPPPSESYGNPSPVDQVLAVSGSASSLTALQIDAAVR 199 S F +G SGVEYAL QWWRE G +P SY +P Q+LA+SGSAS L++ QIDAA+ Sbjct: 243 SVFTIGSSGVEYALTQWWRELGSLPETQRSYDRIAPTTQILAISGSASPLSSAQIDAAIT 302 Query: 200 AGFAEVPLDAADLLDPDKHGVVATQVVRSATSLLKEGRSVILHTARGPDDPRIGKMIEKF 259 AGFAE+ +DA L+ D A + +LL EGRSVI+HTARGP D RI +M+E F Sbjct: 303 AGFAEIAIDAKALVREDSWQRTAQNIAVRTIALLSEGRSVIMHTARGPSDTRIAEMLEYF 362 Query: 260 VGGGMSRELAKHEGGRNLGRKLGQLVSDILEAVPLKRLLLSGGDTSSQVAQLLDIDALEI 319 G++ + A+H+GGR LG++LG + +D+LEA L+RL+LSGGDTSSQV Q+L DALEI Sbjct: 363 TARGLTHDDARHQGGRLLGQRLGAIANDVLEATHLQRLVLSGGDTSSQVTQVLGPDALEI 422 Query: 320 AARVSPGVPLCRAGATRGRLDGLQIAFKGGQLGEEKFF 357 R++ G PLCR + + L LQ+A KGGQ+G++ FF Sbjct: 423 DGRLAVGAPLCRVISEKPHLRNLQLALKGGQMGDQNFF 460 >ref|YP_001566657.1| Hrp-dependent type III effector protein [Delftia acidovorans SPH-1] gb|ABX38272.1| type III effector Hrp-dependent outer [Delftia acidovorans SPH-1] Length = 469 Score = 333 bits (855), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 181/356 (50%), Positives = 225/356 (63%), Gaps = 5/356 (1%) Query: 14 KSVFGNDPIPIVGGTPSLQRYCIFGNLFARSGTDGNIYRIDRHPIMSVHPVTPMNESDLA 73 ++ FG+ IPIV P+L RYC FGNLFARSGTD ++RIDRHPIMSVHPVTPM+E+DLA Sbjct: 106 RTAFGDTAIPIVAANPALGRYCAFGNLFARSGTDDRVHRIDRHPIMSVHPVTPMHEADLA 165 Query: 74 VHIGRQAALEIEKLTLPFLDQDHARAAGRFAELAKCAPD-AILLDSVNASQLTQVGRLI- 131 H+ Q A+ + TLP L D AG + A+LLD LT+ GRL+ Sbjct: 166 RHLAGQTAMPVTGFTLPQLTLDRTEMAGELQRVVDATSGGAVLLDGTTTEHLTETGRLLD 225 Query: 132 --ADRAAGNRSA-FVVGPSGVEYALMQWWREAGIIPPPSESYGNPSPVDQVLAVSGSASS 188 A A G ++ F +G SG+EYAL QWW E G +P ++ V QVLAVSGSAS Sbjct: 226 GLAGAAQGRQTPLFCIGGSGLEYALTQWWHETGALPSIPTTHDRFDGVPQVLAVSGSASP 285 Query: 189 LTALQIDAAVRAGFAEVPLDAADLLDPDKHGVVATQVVRSATSLLKEGRSVILHTARGPD 248 L+ALQIDAAV AGF E+P+DAA L+ ++V A L+ G SV+LH+ARGP Sbjct: 286 LSALQIDAAVDAGFVELPVDAAALVAASDPAAELARLVDQALLALRAGHSVMLHSARGPK 345 Query: 249 DPRIGKMIEKFVGGGMSRELAKHEGGRNLGRKLGQLVSDILEAVPLKRLLLSGGDTSSQV 308 DPRI M+ G+SRE A+HEGGR LG + G LV +L A PLKRLLLSGGDTSS V Sbjct: 346 DPRIEAMLSALAKQGISREQARHEGGRLLGHRFGDLVDALLRAHPLKRLLLSGGDTSSHV 405 Query: 309 AQLLDIDALEIAARVSPGVPLCRAGATRGRLDGLQIAFKGGQLGEEKFFEHGWLGK 364 Q L AL + AR++PG PLCRA + L L+IA KGGQ+G+ +F LG Sbjct: 406 TQRLAPQALRVVARLAPGAPLCRAISREPHLAQLEIALKGGQMGQPDYFVKALLGT 461 >ref|YP_001267183.1| type III effector Hrp-dependent outer [Pseudomonas putida F1] gb|ABQ77999.1| type III effector Hrp-dependent outer [Pseudomonas putida F1] Length = 463 Score = 312 bits (799), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 155/336 (46%), Positives = 223/336 (66%), Gaps = 2/336 (0%) Query: 22 IPIVGGTPSLQRYCIFGNLFARSGTDGNIYRIDRHPIMSVHPVTPMNESDLAVHIGRQAA 81 IPI+ G P+L RYC+FGNL+A S TD ++RIDRHPIMS HP+TPM+E+DL++HIG+Q + Sbjct: 122 IPIIAGNPALGRYCVFGNLYALSKTDHKVHRIDRHPIMSAHPITPMHEADLSIHIGKQKS 181 Query: 82 LEIEKLTLPFLDQDHARAAGRFAELAKCAPDAILLDSVNASQLTQVGRLIADRAAGNRSA 141 L I K+ + L++ R + DA+L D + S +T G+++ + ++G Sbjct: 182 LSIGKVDIRQLEK--TAELSRLVDEMTQQHDAVLFDGTDDSHMTTAGQVLTELSSGAAPV 239 Query: 142 FVVGPSGVEYALMQWWREAGIIPPPSESYGNPSPVDQVLAVSGSASSLTALQIDAAVRAG 201 FVVG SGVEY L Q+WR AG+ PP + P DQVL +SGSAS L+ QI+ A+ G Sbjct: 240 FVVGSSGVEYGLTQYWRTAGVFPPRASLAVPLKPADQVLVISGSASPLSQSQIETAIDYG 299 Query: 202 FAEVPLDAADLLDPDKHGVVATQVVRSATSLLKEGRSVILHTARGPDDPRIGKMIEKFVG 261 F E+ +DAA L+ + VV +A S L+EGRSV++HTA+GP D RI +MIE + Sbjct: 300 FVELEVDAAALITESRKSAAIRDVVMAAVSHLEEGRSVLIHTAKGPCDSRIEQMIEAMLQ 359 Query: 262 GGMSRELAKHEGGRNLGRKLGQLVSDILEAVPLKRLLLSGGDTSSQVAQLLDIDALEIAA 321 G+ R+ A+ +GG+ L ++GQ+ +IL+A PLKRL++SGGDTSSQV ++L DAL I + Sbjct: 360 KGLPRDEARVQGGKQLAMEVGQVALEILKAFPLKRLVVSGGDTSSQVTKVLAPDALVIKS 419 Query: 322 RVSPGVPLCRAGATRGRLDGLQIAFKGGQLGEEKFF 357 +SPG PLC+ + L ++IA KGGQ+G+ +F Sbjct: 420 DISPGAPLCQMHSREPYLKDVEIALKGGQMGDRDYF 455 >ref|YP_003243071.1| Hrp-dependent type III effector protein [Paenibacillus sp. Y412MC10] gb|ACX65264.1| type III effector Hrp-dependent outers [Paenibacillus sp. Y412MC10] Length = 468 Score = 239 bits (609), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 138/345 (40%), Positives = 199/345 (57%), Gaps = 8/345 (2%) Query: 22 IPIVGGTPSLQRYCIFGNLFARSGTDGNIYRIDRHPIMSVHPVTPMNESDLAVHIGRQAA 81 +PI+ G P+L+RY +FG+ FA +G DG YR+DRHP MS HP+TPM ESDL +H+ Q Sbjct: 128 VPILVGVPALKRYTLFGHHFAAAGADGGTYRLDRHPTMSRHPITPMRESDLRLHLREQTD 187 Query: 82 LEIEKLTLPFLDQDHARAAGRFAE-LAKCAPDAILLDSVNASQLTQVGRLIADRAAGNRS 140 L + + LD + R L P A+L D ++ +L GRL+ R A R Sbjct: 188 LPVSLMEWTALDGVPEEVSARLEHRLEVGEPAALLFDVIDDPRLEAAGRLMW-REAEERG 246 Query: 141 A--FVVGPSGVEYALMQWWREAGIIPPPSESYGNPSPVDQVLAVSGSASSLTALQIDAAV 198 FV+G SGVEYAL W + G++ P DQ+L VSGS S +T QI A+ Sbjct: 247 TGLFVIGSSGVEYALAAHWAKEGLVDPGGARLQAAEQADQLLVVSGSCSPVTQSQIAWAL 306 Query: 199 RAGFAEVPLDAADLLDPDKHGVVATQVVRSATSLLKEGRSVILHTARGPDDPRIGKMIEK 258 + G+A + + A D L+P + +++ A +++GR++I+++A GP+DP I + E Sbjct: 307 KRGYAGIRVRARDWLEPGRADEARRSLLKQALPFVEQGRNIIVYSADGPEDPAIAALREA 366 Query: 259 FVGGGMSRELAKHEGGRNLGRKLGQLVSDILEAVPLKRLLLSGGDTSSQVAQLLDIDALE 318 GM + GR +G +LG+L SD+L A L+R+L++GGDTS V + L I AL Sbjct: 367 LNARGME----PADSGRLIGTQLGRLTSDLLRATGLRRILIAGGDTSGYVTRELGIYALS 422 Query: 319 IAARVSPGVPLCRAGATRGRLDGLQIAFKGGQLGEEKFFEHGWLG 363 + + PG PLCR + RLDGLQ+A KGGQ+G +FE LG Sbjct: 423 CLSAIVPGGPLCRCYSEDPRLDGLQLALKGGQVGGADYFERVRLG 467 >ref|ZP_02886747.1| type III effector Hrp-dependent protein [Burkholderia graminis C4D1M] gb|EDT07588.1| type III effector Hrp-dependent protein [Burkholderia graminis C4D1M] Length = 470 Score = 239 bits (609), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 144/337 (42%), Positives = 196/337 (58%), Gaps = 15/337 (4%) Query: 23 PIVGGTPSLQRYCIFGNLFARSGTDGNIYRIDRHPIMSVHPVTPMNESDLAVHIGRQAAL 82 P+V G P L+RY +FGNLFA DG YR+DRHP MS HPVTPMNE+DL +H+ RQ A Sbjct: 136 PMVIGAPRLKRYQMFGNLFA--AVDGVGYRLDRHPTMSRHPVTPMNEADLRLHLARQTAR 193 Query: 83 EIEKLTLPFLDQDHARAAGRFAELAKCAPD--AILLDSVNASQLTQVGRLIADRAAGNRS 140 IE + + L+ A A R L CAPD +L+D ++ L + GRL+ ++ Sbjct: 194 RIELIDM--LELRGADVATRVRAL--CAPDMPVVLIDVLDEETLAEAGRLVWEQR--GEG 247 Query: 141 AFVVGPSGVEYALMQWWREAGIIPPPSESYGNPSPVDQVLAVSGSASSLTALQIDAAVRA 200 F SG++YAL WR G++PP + S PV + AVSGS S +TA QI A Sbjct: 248 IFTASSSGLQYALAAHWRARGLLPP-TPSLPAADPVQAIAAVSGSCSPVTAAQIGWARAH 306 Query: 201 GFAEVPLDAADLLDPDKHGVVATQVVRSATSLLKEGRSVILHTARGPDDPRIGKMIEKFV 260 GF LD LD +VV +AT L G SVI+H+A GPDDP + F Sbjct: 307 GFHTERLDLPRALDSRDGAAEIERVVTAATQALTRGISVIVHSAEGPDDP----AVTGFD 362 Query: 261 GGGMSRELAKHEGGRNLGRKLGQLVSDILEAVPLKRLLLSGGDTSSQVAQLLDIDALEIA 320 + A+H+ R +GR L +++ +L++V L R++++GGD+S +VA +L IDAL + Sbjct: 363 AIASAAGFARHDAARKVGRALAEVMRRLLDSVELTRVVVAGGDSSGEVASVLGIDALSVM 422 Query: 321 ARVSPGVPLCRAGATRGRLDGLQIAFKGGQLGEEKFF 357 A + PG PLCRA + R DGLQI KGGQ+G+ FF Sbjct: 423 AGLVPGAPLCRAWSAEPRRDGLQIVLKGGQIGDATFF 459