BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0097.0004.p (257 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08628874.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacter... 301 7e-80 ref|YP_002825150.1| 3-hydroxybutyryl-CoA dehydratase [Sinorhizob... 255 5e-66 gb|AEJ26832.1| Enoyl-CoA hydratase [Paracoccus denitrificans SD1] 244 8e-63 ref|ZP_03570338.1| enoyl-CoA hydratase [Burkholderia multivorans... 218 1e-54 ref|XP_003391442.1| PREDICTED: 3-hydroxypropionyl-coenzyme A deh... 184 9e-45 >ref|ZP_08628874.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C] gb|EGP08233.1| enoyl-CoA hydratase [Bradyrhizobiaceae bacterium SG-6C] Length = 257 Score = 301 bits (770), Expect = 7e-80, Method: Compositional matrix adjust. Identities = 155/254 (61%), Positives = 191/254 (75%) Query: 3 DIIVAKRDGIARVALNRSSARNAVTLAMWRELADIFSHFACDRDVRAVLLAGEGKDFSVG 62 DI VA +D IA V LNR + RNA+TLAMW+ + DIFS D +VRA++L G G++FSVG Sbjct: 3 DIDVAVQDRIAVVTLNRPAERNAMTLAMWKGIVDIFSRLGVDPEVRAIILTGAGRNFSVG 62 Query: 63 ADISEFDSIRDDKRQAADYEVAVDACSGAIAALGKPVVAVISGYCLGGGCHLALACDFRF 122 AD+SEF +R + ++ YEVAVDA S AIA+ KPV+AV+ GYCLGGGCHL++ACDFR+ Sbjct: 63 ADVSEFSKVRSNIAESKAYEVAVDASSDAIASAPKPVIAVLEGYCLGGGCHLSMACDFRY 122 Query: 123 GDRTATIGIPSAKLSIVYGVRSVQRLLSLTGVANAKRILYSADRYRAEQAMSMGLIDEIH 182 D +IGIP+AKLSIVYGVRS QRLL+L G+ANAKR+LY+A+R+RA A MGLID + Sbjct: 123 ADAETSIGIPAAKLSIVYGVRSTQRLLALVGLANAKRVLYAAERFRAGDAARMGLIDHVS 182 Query: 183 DDAPRAAERFLKQLADNAPLSIAGAKYMLNELSTGAGSLDLAMAQQFIDAASDSEDFREG 242 D AA+ +A APLS+AGAK +L LS GAG+LDL A IDAAS SED+ EG Sbjct: 183 TDPMSAAKALAADIAGLAPLSVAGAKRILTGLSMGAGALDLREADSLIDAASASEDYEEG 242 Query: 243 RRAFAEKRAPRFRG 256 RRAFAEKRAP FRG Sbjct: 243 RRAFAEKRAPVFRG 256 >ref|YP_002825150.1| 3-hydroxybutyryl-CoA dehydratase [Sinorhizobium fredii NGR234] gb|ACP24397.1| 3-hydroxybutyryl-CoA dehydratase [Sinorhizobium fredii NGR234] Length = 255 Score = 255 bits (651), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 131/256 (51%), Positives = 178/256 (69%), Gaps = 2/256 (0%) Query: 1 VKDIIVAKRDGIARVALNRSSARNAVTLAMWRELADIFSHFACDRDVRAVLLAGEGKDFS 60 + DI+V + G+A V L+R RNAVT AMWR+LA++F F D VR+++L G G DFS Sbjct: 1 MSDILVENKGGVAVVTLSRPGHRNAVTYAMWRQLAELFRAFDLDPSVRSIILTGAGDDFS 60 Query: 61 VGADISEFDSIRDDKRQAADYEVAVDACSGAIAALGKPVVAVISGYCLGGGCHLALACDF 120 GADISEFD +R + YE+AVDA + AI + KP +A + GYCLGG HLA++CDF Sbjct: 61 AGADISEFDVVRGGLEDSKSYELAVDAAADAIYQVSKPTIAALRGYCLGGAAHLAMSCDF 120 Query: 121 RFGDRTATIGIPSAKLSIVYGVRSVQRLLSLTGVANAKRILYSADRYRAEQAMSMGLIDE 180 R + A GIP+A+LSIVYGV + ++L +L GV AKRIL+SA R+ A +A+ +G ID+ Sbjct: 121 RVAENNAVFGIPAARLSIVYGVSATRKLYALVGVTEAKRILFSASRFDAGRALDIGFIDQ 180 Query: 181 IHDDAPRAAERFLKQLADNAPLSIAGAKYMLNELSTGAGSLDLAMAQQFIDAASDSEDFR 240 + ++A AA +LA+NAPL+I+GAK++LN + GA LD A A + ID AS SED+ Sbjct: 181 VAENALAAAHDRAAELAENAPLTISGAKFILNGCARGA--LDHAAAGRLIDEASASEDYI 238 Query: 241 EGRRAFAEKRAPRFRG 256 EGR+AF +KR PRF G Sbjct: 239 EGRQAFKDKRPPRFLG 254 >gb|AEJ26832.1| Enoyl-CoA hydratase [Paracoccus denitrificans SD1] Length = 257 Score = 244 bits (623), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 132/257 (51%), Positives = 170/257 (66%), Gaps = 2/257 (0%) Query: 1 VKDIIVAKRDGIARVALNRSSARNAVTLAMWRELADIFSHFACDRDVRAVLLAGEGKDFS 60 + DI+V +G+ARV LNR + RNA+T AMW L +F D V+AV+L G G DF+ Sbjct: 1 MTDILVNIEEGVARVTLNRPAQRNALTYAMWNGLDKVFGDLGRDPSVKAVVLTGAGTDFT 60 Query: 61 VGADISEFDSIRDDKRQAADYEVAVDACSGAIAALGKPVVAVISGYCLGGGCHLALACDF 120 GADI+EF IRDD QA YEVAVD AI K V+A GY LGGG H+A++ DF Sbjct: 61 AGADIAEFAEIRDDADQARAYEVAVDLGCDAILRCPKAVIAACKGYTLGGGMHVAMSADF 120 Query: 121 RFGDRTATIGIPSAKLSIVYGVRSVQRLLSLTGVANAKRILYSADRYRAEQAMSMGLIDE 180 RF + A GIP+A+LSIVYGV++ ++L++L G+ AKRILY R+ A A+ +GLID Sbjct: 121 RFAPQEAQFGIPAARLSIVYGVQATRKLMALVGLTEAKRILYGGRRFDAGHALKVGLIDR 180 Query: 181 IHDDAPRAAERFLKQLAD-NAPLSIAGAKYMLNELSTGAGSLDLAMAQQFIDAASDSEDF 239 I D A RF + LA +APL+I GAKY+LN + G G D A++ IDAA+ S D+ Sbjct: 181 ITADPLEEAIRFARDLATGSAPLTIGGAKYILNGAAIG-GGFDADEAERLIDAAAASADY 239 Query: 240 REGRRAFAEKRAPRFRG 256 REGR AFAEKR P+FRG Sbjct: 240 REGRAAFAEKRQPQFRG 256 >ref|ZP_03570338.1| enoyl-CoA hydratase [Burkholderia multivorans CGD2M] ref|ZP_03576982.1| enoyl-CoA hydratase [Burkholderia multivorans CGD2] gb|EEE08559.1| enoyl-CoA hydratase [Burkholderia multivorans CGD2] gb|EEE16245.1| enoyl-CoA hydratase [Burkholderia multivorans CGD2M] Length = 273 Score = 218 bits (554), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 119/246 (48%), Positives = 155/246 (63%) Query: 12 IARVALNRSSARNAVTLAMWRELADIFSHFACDRDVRAVLLAGEGKDFSVGADISEFDSI 71 IA V + R RNA+TL MWR LA + DVRAV+L G F GADI+EFD + Sbjct: 28 IATVTIARPGKRNAMTLDMWRHLAWLAEQLDGRDDVRAVVLTGSQDSFCAGADIAEFDEL 87 Query: 72 RDDKRQAADYEVAVDACSGAIAALGKPVVAVISGYCLGGGCHLALACDFRFGDRTATIGI 131 R + Q DY+ +A AI LGKPV+A I+G+C+GGGC+LALACDFRF AT I Sbjct: 88 RGNVTQVLDYDAIYEAGCRAIETLGKPVIAAINGHCIGGGCNLALACDFRFAVPQATFAI 147 Query: 132 PSAKLSIVYGVRSVQRLLSLTGVANAKRILYSADRYRAEQAMSMGLIDEIHDDAPRAAER 191 P+AKLS+VYG +RLL+L G A A+RIL++ R A A++ GLIDE+HDD AA + Sbjct: 148 PAAKLSVVYGAGGTRRLLNLVGPARARRILFTGARLSARDALNAGLIDEVHDDVYMAARQ 207 Query: 192 FLKQLADNAPLSIAGAKYMLNELSTGAGSLDLAMAQQFIDAASDSEDFREGRRAFAEKRA 251 K + +PLSI GAK +L L T G+ +A+ + A++S D+ E R AF EKR Sbjct: 208 CAKLMTAVSPLSIRGAKAILTALETATGNHVERVAEAAVRRAANSTDYVEARAAFREKRV 267 Query: 252 PRFRGA 257 PRF G+ Sbjct: 268 PRFCGS 273 >ref|XP_003391442.1| PREDICTED: 3-hydroxypropionyl-coenzyme A dehydratase-like [Amphimedon queenslandica] Length = 299 Score = 184 bits (468), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 100/256 (39%), Positives = 145/256 (56%), Gaps = 3/256 (1%) Query: 4 IIVAKRDGIARVALNRSSARNAVTLAMWRELADIFSHFACDRDVRAVLLAGEG-KDFSVG 62 II K G+ R+ LN+ + RNA++ +MW + + F D DVR V+++GEG + FS G Sbjct: 43 IIADKAQGVGRLRLNQPNKRNAISYSMWLGIVEAMKAFEEDPDVRTVVMSGEGGRAFSAG 102 Query: 63 ADISEFDSIRDDKRQAADYEVAVDACSGAIAALGKPVVAVISGYCLGGGCHLALACDFRF 122 ADISEF R Q Y A IA KPV+ I G+C+GGG +AL D R Sbjct: 103 ADISEFQKNRSTADQIEVYNHATHIAYQTIARFPKPVITRIEGFCVGGGMAVALCADLRI 162 Query: 123 GDRTATIGIPSAKLSIVYGVRSVQRLLSLTGVANAKRILYSADRYRAEQAMSMGLIDEI- 181 +T GIP+AKL + Y +Q L+ + G + AK IL++A R+ A +A MGL++ + Sbjct: 163 ATDDSTFGIPAAKLGLGYSHEHLQMLVDIVGPSFAKEILFTAKRFSAAEAQGMGLVNRVV 222 Query: 182 -HDDAPRAAERFLKQLADNAPLSIAGAKYMLNELSTGAGSLDLAMAQQFIDAASDSEDFR 240 + + + +ADNAPL++ K ++EL DL + ++ +DA SED++ Sbjct: 223 ARGEIEAFTFEYAQTIADNAPLTVRSCKTSISELMKDPDERDLLLCREMVDACFASEDYK 282 Query: 241 EGRRAFAEKRAPRFRG 256 EGR AF EKR PRFRG Sbjct: 283 EGRSAFVEKRRPRFRG 298